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The map label for this gene is cafE

Identifier: 15836492

GI number: 15836492

Start: 1094145

End: 1095692

Strand: Reverse

Name: cafE

Synonym: CPj0959

Alternate gene names: 15836492

Gene position: 1095692-1094145 (Counterclockwise)

Preceding gene: 15836497

Following gene: 15836491

Centisome position: 89.33

GC content: 38.31

Gene sequence:

>1548_bases
ATGGAAAATGAAATTTTACTCAACATAGAATCGAAAGAAATTCGCTATGCCCATCTGAAAAATGGTCAACTTTTTGATCT
AACTATAGAGAGAAAGAAAGTTCGTCAGCTCAAAGGCAATATTTATCGAGGTCGTGTTACCAACATTCTGAGAAATATCC
AATCTGCGTTTATTAATATTGATGAGAGAGAGAATGGTTTCATTCATATCTCTGATATTTTAGAGAATTCGAAGAAGTTT
GAACAGATGTTCGATATGGATGTAGATGCTCTTCCCGAAGAAGCTTCTGAAGCTCCTCTTCTTTCTTCTGAGGAAGCTCC
CATTGAAGAATTTCTCAAATTAGACAGCCCCGTTCTCGTTCAGGTAGTCAAAGAGCCTATTGGAAGTAAGGGAGCTCGCT
TAACTTCTAACATCTCCATTCCGGGACGTTATTTGGTACTTTTACCAAACTCACCTCACCGAGGTGTTTCTCGAAAAATT
GAAGATCCCCATATGAGAGAGCAGTTAAAACAGCTCATTCGTTCTTTTGAAATGCCTCAAGACATGGGTTTGATTTGCCG
TACAGCGAGCACTACAGCCTCTACTGAAGCTCTCATTAATGAGGCCCACGACTTGTTACTCACTTGGAAAACAATTTTAG
AAAAATTCTATTCCACAGAGCAACCCTGCTTGCTTTATTCTGAGACGGACATACTAAAAAAAGCTGTGATTACTTGTATC
GATAAGAACTACAAGCGACTCCTTATTGATGATTATGCTACGTATCAAAAGTGCAAACATATGTTAAAAAAATACTCTCC
GGATGCTTCCATCAAGATAGAGTATTATCGGGATTCTATTCCGATGTTTGAGCGTTTCAATATAGAGAAAGAAATTGATA
AGGCGACAAGAAGAAAAATTTGGCTGTCCAGCGGTGGTTATTTGTTTTTTGACAAAACAGAAGCCATGCACACTATCGAC
GTAAATTCGGGAAGAAGCACACAGTTAGAAAGTGGAGTTGAAGAAACTCTGGTCCAAATCAACTTAGAAGCAGCTGAAGA
AATTGCCAGACAGTTGCGTTTACGTAATGTAGGCGGATTAGTGATTATTGACTTTATCGATATGAAATCTCGTAAGAACC
AGCGACGTGTTTTGGAACGATTGAAAGAACATATGAAATACGATGCTGCTCGCTGTACTATTTTAAGCATGAGTGAATTT
GGTCTTGTTGAGATGACCAGACAGAGAAATCGGGAATCTCTAATGCAGACATTATTCACTCTATGTCGCTACTGCAGCGG
CAACGCCATTATTAAAACACCTGAAAGTGTGGTCATCGAGATTGAGAGGGATTTGAAAAAGGTTATTAATCACAAAGAAC
ACTCTCATCTTTGTCTTGTTGTGCATCCAGAAATTGCAAGCTATATGAAACAAGAAAACGATGACAATGAAATGATCAAT
TTGGCTAAGCAATTAAAAGCTAAACTTCAGATCAATACATCGGACTCGGTTCATTTAAACCACTACCAATTCTTCTCTCT
CATTACAGGGGAAAGTATAGATTTATAG

Upstream 100 bases:

>100_bases
TCACGAGCAGTTAGAAATTTAAGTTTCGACGTCTAGAAACCACTCTTTTCCCAAACAGAATAAGTAAGCTCAAGGATATG
GTATAGGCACAAGGAAAGTC

Downstream 100 bases:

>100_bases
GGCAATATACATGCAGTTTTCTAGGTATTTACGCTATGCTTTTGATAACCAGTATCTCCCAGAGCCTTTATATCAGAAAT
TTTCTGTTTTTCATCAGAAT

Product: axial filament protein

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MENEILLNIESKEIRYAHLKNGQLFDLTIERKKVRQLKGNIYRGRVTNILRNIQSAFINIDERENGFIHISDILENSKKF
EQMFDMDVDALPEEASEAPLLSSEEAPIEEFLKLDSPVLVQVVKEPIGSKGARLTSNISIPGRYLVLLPNSPHRGVSRKI
EDPHMREQLKQLIRSFEMPQDMGLICRTASTTASTEALINEAHDLLLTWKTILEKFYSTEQPCLLYSETDILKKAVITCI
DKNYKRLLIDDYATYQKCKHMLKKYSPDASIKIEYYRDSIPMFERFNIEKEIDKATRRKIWLSSGGYLFFDKTEAMHTID
VNSGRSTQLESGVEETLVQINLEAAEEIARQLRLRNVGGLVIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEF
GLVEMTRQRNRESLMQTLFTLCRYCSGNAIIKTPESVVIEIERDLKKVINHKEHSHLCLVVHPEIASYMKQENDDNEMIN
LAKQLKAKLQINTSDSVHLNHYQFFSLITGESIDL

Sequences:

>Translated_515_residues
MENEILLNIESKEIRYAHLKNGQLFDLTIERKKVRQLKGNIYRGRVTNILRNIQSAFINIDERENGFIHISDILENSKKF
EQMFDMDVDALPEEASEAPLLSSEEAPIEEFLKLDSPVLVQVVKEPIGSKGARLTSNISIPGRYLVLLPNSPHRGVSRKI
EDPHMREQLKQLIRSFEMPQDMGLICRTASTTASTEALINEAHDLLLTWKTILEKFYSTEQPCLLYSETDILKKAVITCI
DKNYKRLLIDDYATYQKCKHMLKKYSPDASIKIEYYRDSIPMFERFNIEKEIDKATRRKIWLSSGGYLFFDKTEAMHTID
VNSGRSTQLESGVEETLVQINLEAAEEIARQLRLRNVGGLVIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEF
GLVEMTRQRNRESLMQTLFTLCRYCSGNAIIKTPESVVIEIERDLKKVINHKEHSHLCLVVHPEIASYMKQENDDNEMIN
LAKQLKAKLQINTSDSVHLNHYQFFSLITGESIDL
>Mature_515_residues
MENEILLNIESKEIRYAHLKNGQLFDLTIERKKVRQLKGNIYRGRVTNILRNIQSAFINIDERENGFIHISDILENSKKF
EQMFDMDVDALPEEASEAPLLSSEEAPIEEFLKLDSPVLVQVVKEPIGSKGARLTSNISIPGRYLVLLPNSPHRGVSRKI
EDPHMREQLKQLIRSFEMPQDMGLICRTASTTASTEALINEAHDLLLTWKTILEKFYSTEQPCLLYSETDILKKAVITCI
DKNYKRLLIDDYATYQKCKHMLKKYSPDASIKIEYYRDSIPMFERFNIEKEIDKATRRKIWLSSGGYLFFDKTEAMHTID
VNSGRSTQLESGVEETLVQINLEAAEEIARQLRLRNVGGLVIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEF
GLVEMTRQRNRESLMQTLFTLCRYCSGNAIIKTPESVVIEIERDLKKVINHKEHSHLCLVVHPEIASYMKQENDDNEMIN
LAKQLKAKLQINTSDSVHLNHYQFFSLITGESIDL

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=493, Percent_Identity=34.6855983772819, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1787325, Length=513, Percent_Identity=35.0877192982456, Blast_Score=283, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 59611; Mature: 59611

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEILLNIESKEIRYAHLKNGQLFDLTIERKKVRQLKGNIYRGRVTNILRNIQSAFINI
CCCCEEEEECCCCEEEEEECCCEEEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHEEC
DERENGFIHISDILENSKKFEQMFDMDVDALPEEASEAPLLSSEEAPIEEFLKLDSPVLV
CCCCCCEEEEHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHH
QVVKEPIGSKGARLTSNISIPGRYLVLLPNSPHRGVSRKIEDPHMREQLKQLIRSFEMPQ
HHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DMGLICRTASTTASTEALINEAHDLLLTWKTILEKFYSTEQPCLLYSETDILKKAVITCI
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHH
DKNYKRLLIDDYATYQKCKHMLKKYSPDASIKIEYYRDSIPMFERFNIEKEIDKATRRKI
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHEE
WLSSGGYLFFDKTEAMHTIDVNSGRSTQLESGVEETLVQINLEAAEEIARQLRLRNVGGL
EEECCCEEEEECCCCEEEEECCCCCCCHHHCCHHHHHHEEEHHHHHHHHHHHHHHCCCCE
VIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEFGLVEMTRQRNRESLMQTLFT
EEEEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LCRYCSGNAIIKTPESVVIEIERDLKKVINHKEHSHLCLVVHPEIASYMKQENDDNEMIN
HHHHHCCCEEEECCCHHEEEEHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCHHHHH
LAKQLKAKLQINTSDSVHLNHYQFFSLITGESIDL
HHHHHHHEEEECCCCCEEECCEEEEEEECCCCCCC
>Mature Secondary Structure
MENEILLNIESKEIRYAHLKNGQLFDLTIERKKVRQLKGNIYRGRVTNILRNIQSAFINI
CCCCEEEEECCCCEEEEEECCCEEEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHEEC
DERENGFIHISDILENSKKFEQMFDMDVDALPEEASEAPLLSSEEAPIEEFLKLDSPVLV
CCCCCCEEEEHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHH
QVVKEPIGSKGARLTSNISIPGRYLVLLPNSPHRGVSRKIEDPHMREQLKQLIRSFEMPQ
HHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DMGLICRTASTTASTEALINEAHDLLLTWKTILEKFYSTEQPCLLYSETDILKKAVITCI
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHH
DKNYKRLLIDDYATYQKCKHMLKKYSPDASIKIEYYRDSIPMFERFNIEKEIDKATRRKI
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHEE
WLSSGGYLFFDKTEAMHTIDVNSGRSTQLESGVEETLVQINLEAAEEIARQLRLRNVGGL
EEECCCEEEEECCCCEEEEECCCCCCCHHHCCHHHHHHEEEHHHHHHHHHHHHHHCCCCE
VIIDFIDMKSRKNQRRVLERLKEHMKYDAARCTILSMSEFGLVEMTRQRNRESLMQTLFT
EEEEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LCRYCSGNAIIKTPESVVIEIERDLKKVINHKEHSHLCLVVHPEIASYMKQENDDNEMIN
HHHHHCCCEEEECCCHHEEEEHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCHHHHH
LAKQLKAKLQINTSDSVHLNHYQFFSLITGESIDL
HHHHHHHEEEECCCCCEEECCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]