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The map label for this gene is dnaG

Identifier: 15836475

GI number: 15836475

Start: 1075334

End: 1077106

Strand: Direct

Name: dnaG

Synonym: CPj0942

Alternate gene names: 15836475

Gene position: 1075334-1077106 (Clockwise)

Preceding gene: 15836472

Following gene: 15836476

Centisome position: 87.67

GC content: 40.27

Gene sequence:

>1773_bases
ATGTATACGGAAGAGAGCTTAGATAACCTGAGACACAGTATAGATATTGTGGATGTCCTTTCGGAACACATCCATTTGAA
GCGCTCCGGTGCAACATACAAGGCATGTTGTCCTTTTCATACAGAGAAAACACCTTCGTTCATAGTCAATCCTGCGGGTG
CACACTACCACTGCTTTGGATGTGGTGCACATGGAGATGCCATTGGCTTCCTCATGCAGCACTTGGGATACTCTTTTACT
GAAGCCATTTTGGTATTATCTAAAAAATTTCAAGTAGACCTAGTTCTCCAACCCAAGGATTCCGGATACACCCCTCCTCA
AGGACTGAAAGAAGAATTACGTCACATCAACAGTGAAGCTGAAACTTTTTTCCGTTATTGCTTGTATCACCTTCCAGAAG
CGAGACACGCTTTGCAGTATTTATACCATCGGGGATTTTCTCCAGATACAATCGATCGATTCCATTTGGGTTATGGACCA
GAACAATCTCTTTTTCTACAAGCCATGGAAGAAAGGAAAATCTCACAAGAACAACTGCATACTGCAGGGTTTTTTGGGAA
TAAATGGTTTTTGTTTGCACGAAGAATCATCTTTCCTGTCCACGATGCGCTAGGACATACCATTGGATTTTCCGCTAGGA
AATTTTTAGAAAACTCCCAAGGGGGTAAGTATGTCAACACTCCAGAAACTCCTATATTCAAAAAGTCCAGGATCCTCTTT
GGGTTAAATTTTTCACGTAGGAGAATAGCCAAAGAAAAAAAAGTCATCCTAGTGGAAGGACAGGCCGATTGCCTGCAAAT
GATAGATTCAGGATTTAATTGTACAGTGGCAGCTCAAGGTACAGCATTTACAGAAGAACATGTGAAAGAGTTAAGTAAAT
TAGGAGTTTTAAAAGTCTTCCTACTCTTCGACAGTGATGAAGCAGGAAATAAAGCAGCATTACGTGTTGGGGATCTTTGT
CAAACTGCTCAGATGTCCGTATTTGTCTGTAAGCTACCACAAGGCCATGATCCCGATTCTTTTCTTATGCAACGAGGAAG
CTCAGGACTCATTGCTTTATTAGAGCAAAGCCAAGACTATCTTACGTTTTTGATCAGTGAAAAAATGAGTTCTTACCCGA
AGTTTGGCCCTAGAGAAAAGGCTCTTCTAGTTGAAGAAGCGATTCGTCAGATCAAGCATTGGGGGAGTCCTATTCTTGTA
TATGAGCATTTAAAACAACTAGCCTCCTTAATGATGGTTCCAGAAGACATGGTATTGTCTTTAGCAAACCCTCAGGTAAC
AGCCGAACCACAAAATATTCCCATAAAACAAAAAGTTCCCAAGATACATCCTCATATTGTGATGGAAACAGATATCTTGC
GTTGTATGCTTTTTTGTGGATCCAATACTAAAATTCTCTACACAGCGCAATTCTACTTTGTCCCGGAGGATTTCAAACAT
CCCGAATGTAGGAAATTGTTTGCGTTTATGATTTCCTATTACGAAAAATATCGGAAAAATGTTCCCTTTGATGAAGCCTG
TCAGGTACTTTCTGATTCTCAGATTCTTCAACTGTTAACCAAGCGGCGCCTAAACACAGAAGCTCTCGATACTATCTTCG
TACAATCTCTTCAAAAAATGGCAGATAGGAGATGGCGAGAGCAATGCAAACCTCTCTCTCTTAACCAAAATATTCAGGAT
AAAAAGCTTGAGATTTTGGAAGACTATGTTCAATTGCGTAAAGATAGAACAATAATCACACTTCTAGATCCAGAAAGTGA
GCTCATTCCTTAA

Upstream 100 bases:

>100_bases
TAATAATTGGGGAGAGTCTCTTTATTTTATAGAGCCTCAGTCTTTTTAAACTAAGAAATCTGTAAGTTAAAATTGCAGCA
TAACCAAGTTGCGTACCGCT

Downstream 100 bases:

>100_bases
CCCAGCTCTTCTTTTGTAAAGAATTCTTGTTTTTCTAAATCCCCCGGATTTCTTAGATGTTTCCTAATTTCCTTGGAGGA
GGAACCTGCAATGATGTGCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MYTEESLDNLRHSIDIVDVLSEHIHLKRSGATYKACCPFHTEKTPSFIVNPAGAHYHCFGCGAHGDAIGFLMQHLGYSFT
EAILVLSKKFQVDLVLQPKDSGYTPPQGLKEELRHINSEAETFFRYCLYHLPEARHALQYLYHRGFSPDTIDRFHLGYGP
EQSLFLQAMEERKISQEQLHTAGFFGNKWFLFARRIIFPVHDALGHTIGFSARKFLENSQGGKYVNTPETPIFKKSRILF
GLNFSRRRIAKEKKVILVEGQADCLQMIDSGFNCTVAAQGTAFTEEHVKELSKLGVLKVFLLFDSDEAGNKAALRVGDLC
QTAQMSVFVCKLPQGHDPDSFLMQRGSSGLIALLEQSQDYLTFLISEKMSSYPKFGPREKALLVEEAIRQIKHWGSPILV
YEHLKQLASLMMVPEDMVLSLANPQVTAEPQNIPIKQKVPKIHPHIVMETDILRCMLFCGSNTKILYTAQFYFVPEDFKH
PECRKLFAFMISYYEKYRKNVPFDEACQVLSDSQILQLLTKRRLNTEALDTIFVQSLQKMADRRWREQCKPLSLNQNIQD
KKLEILEDYVQLRKDRTIITLLDPESELIP

Sequences:

>Translated_590_residues
MYTEESLDNLRHSIDIVDVLSEHIHLKRSGATYKACCPFHTEKTPSFIVNPAGAHYHCFGCGAHGDAIGFLMQHLGYSFT
EAILVLSKKFQVDLVLQPKDSGYTPPQGLKEELRHINSEAETFFRYCLYHLPEARHALQYLYHRGFSPDTIDRFHLGYGP
EQSLFLQAMEERKISQEQLHTAGFFGNKWFLFARRIIFPVHDALGHTIGFSARKFLENSQGGKYVNTPETPIFKKSRILF
GLNFSRRRIAKEKKVILVEGQADCLQMIDSGFNCTVAAQGTAFTEEHVKELSKLGVLKVFLLFDSDEAGNKAALRVGDLC
QTAQMSVFVCKLPQGHDPDSFLMQRGSSGLIALLEQSQDYLTFLISEKMSSYPKFGPREKALLVEEAIRQIKHWGSPILV
YEHLKQLASLMMVPEDMVLSLANPQVTAEPQNIPIKQKVPKIHPHIVMETDILRCMLFCGSNTKILYTAQFYFVPEDFKH
PECRKLFAFMISYYEKYRKNVPFDEACQVLSDSQILQLLTKRRLNTEALDTIFVQSLQKMADRRWREQCKPLSLNQNIQD
KKLEILEDYVQLRKDRTIITLLDPESELIP
>Mature_590_residues
MYTEESLDNLRHSIDIVDVLSEHIHLKRSGATYKACCPFHTEKTPSFIVNPAGAHYHCFGCGAHGDAIGFLMQHLGYSFT
EAILVLSKKFQVDLVLQPKDSGYTPPQGLKEELRHINSEAETFFRYCLYHLPEARHALQYLYHRGFSPDTIDRFHLGYGP
EQSLFLQAMEERKISQEQLHTAGFFGNKWFLFARRIIFPVHDALGHTIGFSARKFLENSQGGKYVNTPETPIFKKSRILF
GLNFSRRRIAKEKKVILVEGQADCLQMIDSGFNCTVAAQGTAFTEEHVKELSKLGVLKVFLLFDSDEAGNKAALRVGDLC
QTAQMSVFVCKLPQGHDPDSFLMQRGSSGLIALLEQSQDYLTFLISEKMSSYPKFGPREKALLVEEAIRQIKHWGSPILV
YEHLKQLASLMMVPEDMVLSLANPQVTAEPQNIPIKQKVPKIHPHIVMETDILRCMLFCGSNTKILYTAQFYFVPEDFKH
PECRKLFAFMISYYEKYRKNVPFDEACQVLSDSQILQLLTKRRLNTEALDTIFVQSLQKMADRRWREQCKPLSLNQNIQD
KKLEILEDYVQLRKDRTIITLLDPESELIP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=443, Percent_Identity=31.1512415349887, Blast_Score=208, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_CHLPN (Q9Z6W4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A81525
- PIR:   D86608
- PIR:   E72015
- RefSeq:   NP_225137.1
- RefSeq:   NP_300999.1
- RefSeq:   NP_445456.1
- RefSeq:   NP_877249.1
- ProteinModelPortal:   Q9Z6W4
- SMR:   Q9Z6W4
- GeneID:   1467656
- GeneID:   894685
- GeneID:   919704
- GeneID:   963379
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0919
- KEGG:   cpn:CPn0942
- KEGG:   cpt:CpB0977
- TIGR:   CP_0919
- HOGENOM:   HBG413863
- OMA:   QAIQYID
- ProtClustDB:   PRK05667
- BioCyc:   CPNE115711:CP_0919-MONOMER
- BioCyc:   CPNE115713:CPN0942-MONOMER
- BioCyc:   CPNE138677:CPJ0942-MONOMER
- BioCyc:   CPNE182082:CPB0977-MONOMER
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 67643; Mature: 67643

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTEESLDNLRHSIDIVDVLSEHIHLKRSGATYKACCPFHTEKTPSFIVNPAGAHYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCEEECCCCCEEEEEE
CGAHGDAIGFLMQHLGYSFTEAILVLSKKFQVDLVLQPKDSGYTPPQGLKEELRHINSEA
CCCCHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCHHH
ETFFRYCLYHLPEARHALQYLYHRGFSPDTIDRFHLGYGPEQSLFLQAMEERKISQEQLH
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHHCCCHHHHH
TAGFFGNKWFLFARRIIFPVHDALGHTIGFSARKFLENSQGGKYVNTPETPIFKKSRILF
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEECCEEEE
GLNFSRRRIAKEKKVILVEGQADCLQMIDSGFNCTVAAQGTAFTEEHVKELSKLGVLKVF
ECCHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEEEE
LLFDSDEAGNKAALRVGDLCQTAQMSVFVCKLPQGHDPDSFLMQRGSSGLIALLEQSQDY
EEECCCCCCCCHHEEHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCHHH
LTFLISEKMSSYPKFGPREKALLVEEAIRQIKHWGSPILVYEHLKQLASLMMVPEDMVLS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHE
LANPQVTAEPQNIPIKQKVPKIHPHIVMETDILRCMLFCGSNTKILYTAQFYFVPEDFKH
ECCCCEECCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCC
PECRKLFAFMISYYEKYRKNVPFDEACQVLSDSQILQLLTKRRLNTEALDTIFVQSLQKM
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ADRRWREQCKPLSLNQNIQDKKLEILEDYVQLRKDRTIITLLDPESELIP
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MYTEESLDNLRHSIDIVDVLSEHIHLKRSGATYKACCPFHTEKTPSFIVNPAGAHYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCEEECCCCCEEEEEE
CGAHGDAIGFLMQHLGYSFTEAILVLSKKFQVDLVLQPKDSGYTPPQGLKEELRHINSEA
CCCCHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCHHH
ETFFRYCLYHLPEARHALQYLYHRGFSPDTIDRFHLGYGPEQSLFLQAMEERKISQEQLH
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHHCCCHHHHH
TAGFFGNKWFLFARRIIFPVHDALGHTIGFSARKFLENSQGGKYVNTPETPIFKKSRILF
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEECCEEEE
GLNFSRRRIAKEKKVILVEGQADCLQMIDSGFNCTVAAQGTAFTEEHVKELSKLGVLKVF
ECCHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEEEE
LLFDSDEAGNKAALRVGDLCQTAQMSVFVCKLPQGHDPDSFLMQRGSSGLIALLEQSQDY
EEECCCCCCCCHHEEHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCHHH
LTFLISEKMSSYPKFGPREKALLVEEAIRQIKHWGSPILVYEHLKQLASLMMVPEDMVLS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHE
LANPQVTAEPQNIPIKQKVPKIHPHIVMETDILRCMLFCGSNTKILYTAQFYFVPEDFKH
ECCCCEECCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCC
PECRKLFAFMISYYEKYRKNVPFDEACQVLSDSQILQLLTKRRLNTEALDTIFVQSLQKM
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ADRRWREQCKPLSLNQNIQDKKLEILEDYVQLRKDRTIITLLDPESELIP
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362