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The map label for this gene is murF

Identifier: 15836432

GI number: 15836432

Start: 1030196

End: 1031539

Strand: Direct

Name: murF

Synonym: CPj0899

Alternate gene names: 15836432

Gene position: 1030196-1031539 (Clockwise)

Preceding gene: 15836429

Following gene: 15836433

Centisome position: 83.99

GC content: 41.74

Gene sequence:

>1344_bases
ATGCGAGCTATGTTGCTTGAAGATTGGGTCTCTCTGATGCTATCAGATGTCTCTTGTCCGAAGTGTGATAAAAAAATTAC
AGGATTTGCTATAGATAGCCAGAAAGTGCAGCCTGGGGATCTATTTTTTGCCCTTCCTGGAAATGCAACAGACGGTCATC
AATTTTTAAAACATGCCGCAACCGCAGGAGCTGTTGCCGCCGTGGTTTCCCATGACTACCAAGGAGATAGCTTTGGTCTA
GAATTGATCCGTGTTGATGATACGAAATCTGCTTTGCAAGAAGCAGGATCCAATCAATGCAATTTATTCCAAGGCACCCT
TGTTGGAATCACAGGATCTGTAGGGAAAACAACAACCAAAGAATTCTCAAAAACAATCCTAAGCTCCATCTATAAAACTC
ACGCAAGCCCTAAAAGTTATAATTCACAGTTGACGGTACCTTTAAGCCTCTTGATGGCGGAAGGGGACGAAGATGTGATG
ATTTTAGAGATGGGGGTCTCTGAACCAGGAAATATGCAAGATCTCCTTCGGATCGTCCAGCCAGAGATCGCAGTGATCAC
GCATATTAATGACCAACATGCGATGCATTTCCCCCAAGGCATCCAAGAGATCCTAAAAGAAAAAAGCTATATTCTACAAA
AAAGTAAACTACAACTGCTCCCCAAAGATTCTCCATATTACCTAGATTTAAGGTCCTGTTCTCCTACTGCCGAAAAATTT
TCTTTTTCTTTTAACGATCCCCTTGCAGATTTCTGCTACAAGGCAATTAGTGGAGACTCTGTAGTCATCCAAACTCCTGA
AGAAAATTATTGTCTTCCGATAGCTTTTTCTTACAAGCCTGCATATACCAACTTATTAATTGCTGTAGCACTCTCTTGGA
TCTTAGAAGTTCCAGAAGAGGGAGTGATACGCTCTCTACCTGAACTGAAGTTGCCTCCTATGCGCTTTGAACATAGCATG
AGAAATGGAATGCAGGTCATCAACGATGCATACAATGCATGTCCAGAAGCTATGATCGCTGCTCTCGATGCTCTACCTTT
ACCAAGTGATGGAGGGAAAATCATCTTAATTTTAGGCCATATGGCTGAATTAGGCAGGTATTCAGAAGAAGGACATGCTT
TAGTAGCTGAAAAAGCAGCTTCTCGAGGAGATATGATATTTTTTATTGGGGAAAAGTGGATCCCAGTTCAAAGTGTTTTA
AAAAGCTATTCTTGTGAAGTGAGCTTTTTCTCCTCAGCTCAAGATGTTAAAGACATTTTGAAGCAAGTGGCACGCTATGG
GGACGTGATTCTACTGAAAGGTTCTCGTGCCCTCGCTCTCGAATCCTTATTAGCTTGTTTTTAA

Upstream 100 bases:

>100_bases
TCTTGTCTGCATTTTAAATATAGTACTTTTTAGTGTAGGTCCATCCTTCTGGTAGGGAGTTCAGACTTTCTAAAACCATA
GATGCTGTAGGCAAAATTAT

Downstream 100 bases:

>100_bases
TTTCTTAGGAGCAAGTATGATCCCCTTAATTCCAATGTTTCTAAAACAGTCTCTATTTTTTTCTTTGGCTTTGACAGGGA
TGACCACCCTTGTGCTTACA

Product: muramoyl-DAP ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MRAMLLEDWVSLMLSDVSCPKCDKKITGFAIDSQKVQPGDLFFALPGNATDGHQFLKHAATAGAVAAVVSHDYQGDSFGL
ELIRVDDTKSALQEAGSNQCNLFQGTLVGITGSVGKTTTKEFSKTILSSIYKTHASPKSYNSQLTVPLSLLMAEGDEDVM
ILEMGVSEPGNMQDLLRIVQPEIAVITHINDQHAMHFPQGIQEILKEKSYILQKSKLQLLPKDSPYYLDLRSCSPTAEKF
SFSFNDPLADFCYKAISGDSVVIQTPEENYCLPIAFSYKPAYTNLLIAVALSWILEVPEEGVIRSLPELKLPPMRFEHSM
RNGMQVINDAYNACPEAMIAALDALPLPSDGGKIILILGHMAELGRYSEEGHALVAEKAASRGDMIFFIGEKWIPVQSVL
KSYSCEVSFFSSAQDVKDILKQVARYGDVILLKGSRALALESLLACF

Sequences:

>Translated_447_residues
MRAMLLEDWVSLMLSDVSCPKCDKKITGFAIDSQKVQPGDLFFALPGNATDGHQFLKHAATAGAVAAVVSHDYQGDSFGL
ELIRVDDTKSALQEAGSNQCNLFQGTLVGITGSVGKTTTKEFSKTILSSIYKTHASPKSYNSQLTVPLSLLMAEGDEDVM
ILEMGVSEPGNMQDLLRIVQPEIAVITHINDQHAMHFPQGIQEILKEKSYILQKSKLQLLPKDSPYYLDLRSCSPTAEKF
SFSFNDPLADFCYKAISGDSVVIQTPEENYCLPIAFSYKPAYTNLLIAVALSWILEVPEEGVIRSLPELKLPPMRFEHSM
RNGMQVINDAYNACPEAMIAALDALPLPSDGGKIILILGHMAELGRYSEEGHALVAEKAASRGDMIFFIGEKWIPVQSVL
KSYSCEVSFFSSAQDVKDILKQVARYGDVILLKGSRALALESLLACF
>Mature_447_residues
MRAMLLEDWVSLMLSDVSCPKCDKKITGFAIDSQKVQPGDLFFALPGNATDGHQFLKHAATAGAVAAVVSHDYQGDSFGL
ELIRVDDTKSALQEAGSNQCNLFQGTLVGITGSVGKTTTKEFSKTILSSIYKTHASPKSYNSQLTVPLSLLMAEGDEDVM
ILEMGVSEPGNMQDLLRIVQPEIAVITHINDQHAMHFPQGIQEILKEKSYILQKSKLQLLPKDSPYYLDLRSCSPTAEKF
SFSFNDPLADFCYKAISGDSVVIQTPEENYCLPIAFSYKPAYTNLLIAVALSWILEVPEEGVIRSLPELKLPPMRFEHSM
RNGMQVINDAYNACPEAMIAALDALPLPSDGGKIILILGHMAELGRYSEEGHALVAEKAASRGDMIFFIGEKWIPVQSVL
KSYSCEVSFFSSAQDVKDILKQVARYGDVILLKGSRALALESLLACF

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=437, Percent_Identity=27.4599542334096, Blast_Score=118, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49017; Mature: 49017

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMLLEDWVSLMLSDVSCPKCDKKITGFAIDSQKVQPGDLFFALPGNATDGHQFLKHAA
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHH
TAGAVAAVVSHDYQGDSFGLELIRVDDTKSALQEAGSNQCNLFQGTLVGITGSVGKTTTK
HHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEECEEEEEECCCCCCHHH
EFSKTILSSIYKTHASPKSYNSQLTVPLSLLMAEGDEDVMILEMGVSEPGNMQDLLRIVQ
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
PEIAVITHINDQHAMHFPQGIQEILKEKSYILQKSKLQLLPKDSPYYLDLRSCSPTAEKF
CCEEEEEECCCCCCHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHC
SFSFNDPLADFCYKAISGDSVVIQTPEENYCLPIAFSYKPAYTNLLIAVALSWILEVPEE
EECCCCCHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCC
GVIRSLPELKLPPMRFEHSMRNGMQVINDAYNACPEAMIAALDALPLPSDGGKIILILGH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEEECC
MAELGRYSEEGHALVAEKAASRGDMIFFIGEKWIPVQSVLKSYSCEVSFFSSAQDVKDIL
HHHHCCCCCCCCEEHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCHHHHHHHH
KQVARYGDVILLKGSRALALESLLACF
HHHHHCCCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MRAMLLEDWVSLMLSDVSCPKCDKKITGFAIDSQKVQPGDLFFALPGNATDGHQFLKHAA
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHH
TAGAVAAVVSHDYQGDSFGLELIRVDDTKSALQEAGSNQCNLFQGTLVGITGSVGKTTTK
HHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEECEEEEEECCCCCCHHH
EFSKTILSSIYKTHASPKSYNSQLTVPLSLLMAEGDEDVMILEMGVSEPGNMQDLLRIVQ
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
PEIAVITHINDQHAMHFPQGIQEILKEKSYILQKSKLQLLPKDSPYYLDLRSCSPTAEKF
CCEEEEEECCCCCCHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHC
SFSFNDPLADFCYKAISGDSVVIQTPEENYCLPIAFSYKPAYTNLLIAVALSWILEVPEE
EECCCCCHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCC
GVIRSLPELKLPPMRFEHSMRNGMQVINDAYNACPEAMIAALDALPLPSDGGKIILILGH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEEEECC
MAELGRYSEEGHALVAEKAASRGDMIFFIGEKWIPVQSVLKSYSCEVSFFSSAQDVKDIL
HHHHCCCCCCCCEEHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCHHHHHHHH
KQVARYGDVILLKGSRALALESLLACF
HHHHHCCCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]