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The map label for this gene is dagA_2

Identifier: 15836409

GI number: 15836409

Start: 993465

End: 994841

Strand: Direct

Name: dagA_2

Synonym: CPj0876

Alternate gene names: 15836409

Gene position: 993465-994841 (Clockwise)

Preceding gene: 15836408

Following gene: 15836410

Centisome position: 81.0

GC content: 43.86

Gene sequence:

>1377_bases
ATGTTATATTTTATAGAACAGCTAAATAAACTGAGTACGTCGTTTTGTGTATTCCCTATGATCTTATTGTTAGGGGGGTT
CTTGACATGGAAATTACGCGGTTTACAGTTCCACGGGTTAAAGCTCGGCTTTAACTTGATGCTTCAAAATAAATTGGATG
ATAGTTCATCAAAAGCTAACGAAGTTTCTTCGTACGAAGCTGTAGCCGGAATCCTAGCAGGAAATTTTGGCACGGGAAAT
ATCGCCGGAATGGCTGTCGCCTTAGCTTGTGGAGGTCCAGGCGCCCTGGTCTGGGTCTGGCTTGCAGCCCTCCTCGGAGC
TATCGTCCAATATGCTGGCTCCTATTTAGGTTCAAAATATAGGAAACCTGAAGGAAATACAGGAGAATTTATAGGAGGAC
CCATAGCCTGCCTCGCTTTTGGCATGCGTAAAAAAATACTCGCAGGATTCTTTGCTTTATTCACTATCATGACAGCCTTC
TGTGCAGGAAACTGTGTTCAGGTAAGTTGTATCGTTCCTCTCTGTGCAGAAGGAACTCCAGGAAAACTCCTCGTTGGAAT
TCTACTAGCTCTCGTAGTGATCCCCGTGTTAGCAGGAGGAAATAACCGTATATTAAGATTCTCTGCTCGTGTGATTCCCT
TCATAGCAGGATTTTACTGTATTTCTTGCGGGATCATTCTCTTCCAACATGCCTCGGCTATTCTTCCCGCAATCAAACTG
ATATGCTCTTCAGCATTCGGCATTAAAGCCGGACTCGCTGGAATCGGAGGCTATACTCTTTCGCAAGTCATCTCTACAGG
GATTAACCGTGCTGTCATGGCTACAGATTGCGGCAGCGGAATGGTATCTATTTTGCAAGCAAATACAAAAAGCAAAAATC
CTGTTGTAGACGGACTCGTTACTCTAGTCCCCCCAGTCATTGTGATGGTTGTTTGCTCTATTACAATGCTCGTTCTAATT
GTCTCAGGAGCTTACAGCTCAGGAGCCCAAGGAACTCTAATGGTCATGAGTGCCTTTAAAAATAGCCTCGGCTCTCTAGG
TAGTGTGATTGTTATTCTCGCTATGGCCCTATTCGGATATACAACAATATTGACATGGTTTGCTTGCGCAGAAAAAAGTT
TACAATATATGATCCCAGGAAGACGAGCAAATCTATGGTTGAAGGCTATATACGTCTTGATCATTCCTCTAGGGGGTGTT
ATCGATATGCGTATGATTTGGGCCTTATCTGACACAGGTTTTTCTGGTATGGTCATTCTGAACTGCATAGCTCTAATCGC
CTTACTGAAAGATGTACTATCCACAAACCGCGATGTTGCTTTGCTTAAAGAACGCGAGTGCTCTGTTGCAGATCCTGTGC
GTAATCTAGATGCTTAA

Upstream 100 bases:

>100_bases
AACTAAGCTTCCTTTCCCCAAAACAATAGGGAAGGGAAGCCCAGAAGTTTTTCTCTTTGGCCTTCCTGAATTTTCATAAT
CAATCGCAACAGGAGAGACA

Downstream 100 bases:

>100_bases
AGGAGAAGAATCATGCAATTATTGTCCCCAGCGTTTGCTTATGGCGCCCCAATTCCTAAGAAGTATACATGCCAAGGCGC
AGGGATTTCCCCTCCCTTGA

Product: D-alanine/glycine permease

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MLYFIEQLNKLSTSFCVFPMILLLGGFLTWKLRGLQFHGLKLGFNLMLQNKLDDSSSKANEVSSYEAVAGILAGNFGTGN
IAGMAVALACGGPGALVWVWLAALLGAIVQYAGSYLGSKYRKPEGNTGEFIGGPIACLAFGMRKKILAGFFALFTIMTAF
CAGNCVQVSCIVPLCAEGTPGKLLVGILLALVVIPVLAGGNNRILRFSARVIPFIAGFYCISCGIILFQHASAILPAIKL
ICSSAFGIKAGLAGIGGYTLSQVISTGINRAVMATDCGSGMVSILQANTKSKNPVVDGLVTLVPPVIVMVVCSITMLVLI
VSGAYSSGAQGTLMVMSAFKNSLGSLGSVIVILAMALFGYTTILTWFACAEKSLQYMIPGRRANLWLKAIYVLIIPLGGV
IDMRMIWALSDTGFSGMVILNCIALIALLKDVLSTNRDVALLKERECSVADPVRNLDA

Sequences:

>Translated_458_residues
MLYFIEQLNKLSTSFCVFPMILLLGGFLTWKLRGLQFHGLKLGFNLMLQNKLDDSSSKANEVSSYEAVAGILAGNFGTGN
IAGMAVALACGGPGALVWVWLAALLGAIVQYAGSYLGSKYRKPEGNTGEFIGGPIACLAFGMRKKILAGFFALFTIMTAF
CAGNCVQVSCIVPLCAEGTPGKLLVGILLALVVIPVLAGGNNRILRFSARVIPFIAGFYCISCGIILFQHASAILPAIKL
ICSSAFGIKAGLAGIGGYTLSQVISTGINRAVMATDCGSGMVSILQANTKSKNPVVDGLVTLVPPVIVMVVCSITMLVLI
VSGAYSSGAQGTLMVMSAFKNSLGSLGSVIVILAMALFGYTTILTWFACAEKSLQYMIPGRRANLWLKAIYVLIIPLGGV
IDMRMIWALSDTGFSGMVILNCIALIALLKDVLSTNRDVALLKERECSVADPVRNLDA
>Mature_458_residues
MLYFIEQLNKLSTSFCVFPMILLLGGFLTWKLRGLQFHGLKLGFNLMLQNKLDDSSSKANEVSSYEAVAGILAGNFGTGN
IAGMAVALACGGPGALVWVWLAALLGAIVQYAGSYLGSKYRKPEGNTGEFIGGPIACLAFGMRKKILAGFFALFTIMTAF
CAGNCVQVSCIVPLCAEGTPGKLLVGILLALVVIPVLAGGNNRILRFSARVIPFIAGFYCISCGIILFQHASAILPAIKL
ICSSAFGIKAGLAGIGGYTLSQVISTGINRAVMATDCGSGMVSILQANTKSKNPVVDGLVTLVPPVIVMVVCSITMLVLI
VSGAYSSGAQGTLMVMSAFKNSLGSLGSVIVILAMALFGYTTILTWFACAEKSLQYMIPGRRANLWLKAIYVLIIPLGGV
IDMRMIWALSDTGFSGMVILNCIALIALLKDVLSTNRDVALLKERECSVADPVRNLDA

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=451, Percent_Identity=26.1640798226164, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48272; Mature: 48272

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYFIEQLNKLSTSFCVFPMILLLGGFLTWKLRGLQFHGLKLGFNLMLQNKLDDSSSKAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECHHEEEECCCCCCCCHHH
EVSSYEAVAGILAGNFGTGNIAGMAVALACGGPGALVWVWLAALLGAIVQYAGSYLGSKY
HHHHHHHHHHHHHCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKPEGNTGEFIGGPIACLAFGMRKKILAGFFALFTIMTAFCAGNCVQVSCIVPLCAEGTP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
GKLLVGILLALVVIPVLAGGNNRILRFSARVIPFIAGFYCISCGIILFQHASAILPAIKL
HHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICSSAFGIKAGLAGIGGYTLSQVISTGINRAVMATDCGSGMVSILQANTKSKNPVVDGLV
HHHCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHH
TLVPPVIVMVVCSITMLVLIVSGAYSSGAQGTLMVMSAFKNSLGSLGSVIVILAMALFGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTILTWFACAEKSLQYMIPGRRANLWLKAIYVLIIPLGGVIDMRMIWALSDTGFSGMVIL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCHHHHH
NCIALIALLKDVLSTNRDVALLKERECSVADPVRNLDA
HHHHHHHHHHHHHHCCCCEEEHHHCCCCHHCCHHCCCC
>Mature Secondary Structure
MLYFIEQLNKLSTSFCVFPMILLLGGFLTWKLRGLQFHGLKLGFNLMLQNKLDDSSSKAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECHHEEEECCCCCCCCHHH
EVSSYEAVAGILAGNFGTGNIAGMAVALACGGPGALVWVWLAALLGAIVQYAGSYLGSKY
HHHHHHHHHHHHHCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKPEGNTGEFIGGPIACLAFGMRKKILAGFFALFTIMTAFCAGNCVQVSCIVPLCAEGTP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
GKLLVGILLALVVIPVLAGGNNRILRFSARVIPFIAGFYCISCGIILFQHASAILPAIKL
HHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICSSAFGIKAGLAGIGGYTLSQVISTGINRAVMATDCGSGMVSILQANTKSKNPVVDGLV
HHHCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHH
TLVPPVIVMVVCSITMLVLIVSGAYSSGAQGTLMVMSAFKNSLGSLGSVIVILAMALFGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTILTWFACAEKSLQYMIPGRRANLWLKAIYVLIIPLGGVIDMRMIWALSDTGFSGMVIL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCHHHHH
NCIALIALLKDVLSTNRDVALLKERECSVADPVRNLDA
HHHHHHHHHHHHHHCCCCEEEHHHCCCCHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]