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The map label for this gene is gspF

Identifier: 15836350

GI number: 15836350

Start: 926437

End: 927612

Strand: Direct

Name: gspF

Synonym: CPj0817

Alternate gene names: 15836350

Gene position: 926437-927612 (Clockwise)

Preceding gene: 15836349

Following gene: 15836351

Centisome position: 75.53

GC content: 43.96

Gene sequence:

>1176_bases
ATGCCTCGATATCGGTATACATATTTAGATCCCAAAGAGCGAAGGAAACGAGGATATTTGGAAGCCCTTCATATACAAGA
AGCTAGAGAAAAGCTCGCCCAGGAAAATATCCAAGTTTTGGATATTCGTGAGGTCGCCTTACGAAGAATGAGCATTAAAA
GTACCGAGCTCATCGTGTTTACAAAACAGCTCCTCCTCCTCCTACGCTCTGGACTGCCGCTATATGAAAGCTTGGTATCT
CTCCGAGATCAGTATCATGAGCAGAAAATGGGACTTTTGCTCACATCGTTTATGGAAACTCTAAGATCGGGTGGGTCTTT
ATCTCAAGCTATGGCAGCACATCCGAATATCTTTGATCACTTTTATTGTAGTGGTGTCGCTGCTGGAGAAAGTGTGGGGA
ATCTCGAAGGGTGTCTGCAAAATATTATTGTTGTTCTGGAAGAGCGTGCGCAGATTACCAAGAAGATGGTCGGCGCATTA
AGTTATCCTTGTGTGTTGTTAGTATTTTCTTTTGCCGTGATGCTTTTCTTTTTGTTAGGAGTGATCCCTTCATTAAAAGA
GACCTTTGAAAATATGGAAGTCAAAGGACTAACAAAAATTGTTTTTGGAGTTAGCGACTGTCTCTCCGCATACCGGTATC
TATTTTTAGGATTTGCGAGTGCTTTGATTACCGTTGGAATTTTGATGCGCCATCGCATTCCCTGGAAAAAGATCCTAGAG
AAGCTCTTATTTGCTTTGCCAGGAACCAAGAAGTTTGTTGTTAAGGTAGCGGTGAATCGGTTTTGTTCCGTGGCATCGGC
AATCTTGAAGGGAGGGGGGACCCTAATCGAAGGTCTCGACTTGGGGTGTGACGCAATTCCCTATGACAGACTGAAGACCG
ATATGAGAGATATTGTTCAGGCTGTAATCGGTGGGGGATCTTTAAGTCAGGAGCTTGCTCAGCGCTCTTGGGTTCCCAAG
CTCGCTATAGGGATGATTGCTTTGGGAGAAGAGTCGGGGGATCTTGCCGACGTTTTAGGATATGTAGCCCACATTTATAA
TGAGGATACACAAAAAACGTTGGCTTCGATAACGTCGTGGTGTCAACCCGTGATTCTGATTTTTCTTGGTGGCCTGATCG
GTGTGATCATGTTGGCAATATTGATCCCACTCACAAGCAATATCCAAACATTATAA

Upstream 100 bases:

>100_bases
CTTACCGATCTTAGAGCACGGCATCGCTCTTGCTGTATCTGGTGAGACTACCTTAGCAGAAGTCTTAAGAGTTACCAAGC
GCTGTGATTAGGGAGGGCGG

Downstream 100 bases:

>100_bases
AGTGTGTACTCAGAGGAGTCGGTATGAAAAGACAAAAGAGAAAGCAGTCCATCACATTGATTGAGATGATGGTTGTAATC
ACCCTCATAGGGATTATTGG

Product: general secretion protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MPRYRYTYLDPKERRKRGYLEALHIQEAREKLAQENIQVLDIREVALRRMSIKSTELIVFTKQLLLLLRSGLPLYESLVS
LRDQYHEQKMGLLLTSFMETLRSGGSLSQAMAAHPNIFDHFYCSGVAAGESVGNLEGCLQNIIVVLEERAQITKKMVGAL
SYPCVLLVFSFAVMLFFLLGVIPSLKETFENMEVKGLTKIVFGVSDCLSAYRYLFLGFASALITVGILMRHRIPWKKILE
KLLFALPGTKKFVVKVAVNRFCSVASAILKGGGTLIEGLDLGCDAIPYDRLKTDMRDIVQAVIGGGSLSQELAQRSWVPK
LAIGMIALGEESGDLADVLGYVAHIYNEDTQKTLASITSWCQPVILIFLGGLIGVIMLAILIPLTSNIQTL

Sequences:

>Translated_391_residues
MPRYRYTYLDPKERRKRGYLEALHIQEAREKLAQENIQVLDIREVALRRMSIKSTELIVFTKQLLLLLRSGLPLYESLVS
LRDQYHEQKMGLLLTSFMETLRSGGSLSQAMAAHPNIFDHFYCSGVAAGESVGNLEGCLQNIIVVLEERAQITKKMVGAL
SYPCVLLVFSFAVMLFFLLGVIPSLKETFENMEVKGLTKIVFGVSDCLSAYRYLFLGFASALITVGILMRHRIPWKKILE
KLLFALPGTKKFVVKVAVNRFCSVASAILKGGGTLIEGLDLGCDAIPYDRLKTDMRDIVQAVIGGGSLSQELAQRSWVPK
LAIGMIALGEESGDLADVLGYVAHIYNEDTQKTLASITSWCQPVILIFLGGLIGVIMLAILIPLTSNIQTL
>Mature_390_residues
PRYRYTYLDPKERRKRGYLEALHIQEAREKLAQENIQVLDIREVALRRMSIKSTELIVFTKQLLLLLRSGLPLYESLVSL
RDQYHEQKMGLLLTSFMETLRSGGSLSQAMAAHPNIFDHFYCSGVAAGESVGNLEGCLQNIIVVLEERAQITKKMVGALS
YPCVLLVFSFAVMLFFLLGVIPSLKETFENMEVKGLTKIVFGVSDCLSAYRYLFLGFASALITVGILMRHRIPWKKILEK
LLFALPGTKKFVVKVAVNRFCSVASAILKGGGTLIEGLDLGCDAIPYDRLKTDMRDIVQAVIGGGSLSQELAQRSWVPKL
AIGMIALGEESGDLADVLGYVAHIYNEDTQKTLASITSWCQPVILIFLGGLIGVIMLAILIPLTSNIQTL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=397, Percent_Identity=27.2040302267003, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1786295, Length=356, Percent_Identity=23.5955056179775, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43368; Mature: 43237

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYRYTYLDPKERRKRGYLEALHIQEAREKLAQENIQVLDIREVALRRMSIKSTELIVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHH
TKQLLLLLRSGLPLYESLVSLRDQYHEQKMGLLLTSFMETLRSGGSLSQAMAAHPNIFDH
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHH
FYCSGVAAGESVGNLEGCLQNIIVVLEERAQITKKMVGALSYPCVLLVFSFAVMLFFLLG
HHHHCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIPSLKETFENMEVKGLTKIVFGVSDCLSAYRYLFLGFASALITVGILMRHRIPWKKILE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KLLFALPGTKKFVVKVAVNRFCSVASAILKGGGTLIEGLDLGCDAIPYDRLKTDMRDIVQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHH
AVIGGGSLSQELAQRSWVPKLAIGMIALGEESGDLADVLGYVAHIYNEDTQKTLASITSW
HHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
CQPVILIFLGGLIGVIMLAILIPLTSNIQTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PRYRYTYLDPKERRKRGYLEALHIQEAREKLAQENIQVLDIREVALRRMSIKSTELIVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]