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The map label for this gene is gspE

Identifier: 15836349

GI number: 15836349

Start: 924937

End: 926427

Strand: Direct

Name: gspE

Synonym: CPj0816

Alternate gene names: 15836349

Gene position: 924937-926427 (Clockwise)

Preceding gene: 15836348

Following gene: 15836350

Centisome position: 75.41

GC content: 45.34

Gene sequence:

>1491_bases
ATGGCTGCTAGTATTTTATCTCAGGAGCTTTTGGATATCCTTCCTTATACTTTTTTAAAGAAACACTGTCTTCTCCCTAT
TGAAGAGAGTAGTGAGGCTATTACTATAGCCCATGCTACCGCGACTTCAGTCATTGCTCAAGATGAAGTCAAATTGTTAA
TAAAAAAGCCTGTGCGTTTCGTTCTAAAAGAGGAATCGGAGATTCTGCAGCGCTTACAGCAGCTCTACAGCAATCGGGAA
GGTAATGTTTCCGATATGTTGTTAACAATGAAAGAGGAAGATGGCACTACGATTTCGGAAGAAGAAGATCTTCTGGAGAC
TACGGATACGATCCCAGTCGTACGCTTGTTGAACTGGATTCTGAAAGAAGCGATTGAAGAGCGCGCTTCGGACATTCATT
TTGAGCCTTGTGAGGATTCTATGAGAATCCGCTACCGCATTGATGGTGTGCTTCACGATCGTCATTCCCCACCTTCCCAC
CTGCGTTCGGCATTAACCACTCGGCTTAAAGTCCTCGCAAAGATGGATATTGCGGAGCACCGTCTTCCTCAAGACGGGCG
TATTAAGATCCATATTGGTGGTCAGGAAGTGGACATGCGTGTCAGCACGGTTCCCGTGATTTATGGCGAGCGTGTTGTTC
TTCGTATTTTAGATAAGCGCAATGTCATTTTGGATATCGCGGGCTTGCATATGCCTAAGGGTACCGAAATACTCTTTAAA
GATACCATAACAGCTCCTGAAGGGATCCTTCTGGTTACAGGACCTACAGGCAGTGGGAAAACTACGACCCTCTACAGTGT
ATTACAAGAGCTTAAGGGACCTTTAACAAATATCATGACGATCGAAGATCCTCCAGAGTATAAACTGCCTGGAATTGCTC
AGATTGCTGTGAAGCCTAAAATTGGGCTGACTTTCGCACGAGGGTTACGGCATTTACTGCGTCAAGATCCCGATATCCTT
ATGGTCGGAGAAATCCGAGATCAGGAAACTGCAGAAATCGCAATACAAGCAGCATTGACTGGGCATTTGGTAGTGAGCAC
GCTCCATACGAATGACGCTATTTCTGCGATTCCCCGTCTTCTGGATATGGGGATAGAATCCTATTTGTTATCGGCAACGC
TCGTTGGCGTGGTTGCCCAGAGGCTGGTGCGAACCATTTGTCCCTATTGTAAGGTCGCTTATACTCCTGAGAATCAGGAA
AAATCTTTTCTAGCTTCTCTAGGGAAAGATACAGAAATGCCTTTATATCGGGGGCAAGGGTGCGTGCATTGTTTCCGTTC
CGGATATAAAGGAAGACAGGGAATTTACGAATTTTTACGCCCGAATACACTATTTCGTTCAGAAGTAGCCTCAAACCGCC
CCTATCATATTTTACGAGAAACTGCAGAACAAAACGGATTCTTACCGATCTTAGAGCACGGCATCGCTCTTGCTGTATCT
GGTGAGACTACCTTAGCAGAAGTCTTAAGAGTTACCAAGCGCTGTGATTAG

Upstream 100 bases:

>100_bases
TTAGCAGCTAGTGAGGCTGCAGCACGAGCAGCTCATAAAAAATTAGAGATGTTCCCGGCATCAGGAGTATCTTTATCTCA
GGTAGAGAGGCAAGAATACG

Downstream 100 bases:

>100_bases
GGAGGGCGGATGCCTCGATATCGGTATACATATTTAGATCCCAAAGAGCGAAGGAAACGAGGATATTTGGAAGCCCTTCA
TATACAAGAAGCTAGAGAAA

Product: general secretion protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MAASILSQELLDILPYTFLKKHCLLPIEESSEAITIAHATATSVIAQDEVKLLIKKPVRFVLKEESEILQRLQQLYSNRE
GNVSDMLLTMKEEDGTTISEEEDLLETTDTIPVVRLLNWILKEAIEERASDIHFEPCEDSMRIRYRIDGVLHDRHSPPSH
LRSALTTRLKVLAKMDIAEHRLPQDGRIKIHIGGQEVDMRVSTVPVIYGERVVLRILDKRNVILDIAGLHMPKGTEILFK
DTITAPEGILLVTGPTGSGKTTTLYSVLQELKGPLTNIMTIEDPPEYKLPGIAQIAVKPKIGLTFARGLRHLLRQDPDIL
MVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRLLDMGIESYLLSATLVGVVAQRLVRTICPYCKVAYTPENQE
KSFLASLGKDTEMPLYRGQGCVHCFRSGYKGRQGIYEFLRPNTLFRSEVASNRPYHILRETAEQNGFLPILEHGIALAVS
GETTLAEVLRVTKRCD

Sequences:

>Translated_496_residues
MAASILSQELLDILPYTFLKKHCLLPIEESSEAITIAHATATSVIAQDEVKLLIKKPVRFVLKEESEILQRLQQLYSNRE
GNVSDMLLTMKEEDGTTISEEEDLLETTDTIPVVRLLNWILKEAIEERASDIHFEPCEDSMRIRYRIDGVLHDRHSPPSH
LRSALTTRLKVLAKMDIAEHRLPQDGRIKIHIGGQEVDMRVSTVPVIYGERVVLRILDKRNVILDIAGLHMPKGTEILFK
DTITAPEGILLVTGPTGSGKTTTLYSVLQELKGPLTNIMTIEDPPEYKLPGIAQIAVKPKIGLTFARGLRHLLRQDPDIL
MVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRLLDMGIESYLLSATLVGVVAQRLVRTICPYCKVAYTPENQE
KSFLASLGKDTEMPLYRGQGCVHCFRSGYKGRQGIYEFLRPNTLFRSEVASNRPYHILRETAEQNGFLPILEHGIALAVS
GETTLAEVLRVTKRCD
>Mature_495_residues
AASILSQELLDILPYTFLKKHCLLPIEESSEAITIAHATATSVIAQDEVKLLIKKPVRFVLKEESEILQRLQQLYSNREG
NVSDMLLTMKEEDGTTISEEEDLLETTDTIPVVRLLNWILKEAIEERASDIHFEPCEDSMRIRYRIDGVLHDRHSPPSHL
RSALTTRLKVLAKMDIAEHRLPQDGRIKIHIGGQEVDMRVSTVPVIYGERVVLRILDKRNVILDIAGLHMPKGTEILFKD
TITAPEGILLVTGPTGSGKTTTLYSVLQELKGPLTNIMTIEDPPEYKLPGIAQIAVKPKIGLTFARGLRHLLRQDPDILM
VGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRLLDMGIESYLLSATLVGVVAQRLVRTICPYCKVAYTPENQEK
SFLASLGKDTEMPLYRGQGCVHCFRSGYKGRQGIYEFLRPNTLFRSEVASNRPYHILRETAEQNGFLPILEHGIALAVSG
ETTLAEVLRVTKRCD

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=405, Percent_Identity=48.1481481481481, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=44.9350649350649, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI87082188, Length=224, Percent_Identity=34.8214285714286, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55428; Mature: 55297

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASILSQELLDILPYTFLKKHCLLPIEESSEAITIAHATATSVIAQDEVKLLIKKPVRF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
VLKEESEILQRLQQLYSNREGNVSDMLLTMKEEDGTTISEEEDLLETTDTIPVVRLLNWI
HHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHH
LKEAIEERASDIHFEPCEDSMRIRYRIDGVLHDRHSPPSHLRSALTTRLKVLAKMDIAEH
HHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLPQDGRIKIHIGGQEVDMRVSTVPVIYGERVVLRILDKRNVILDIAGLHMPKGTEILFK
CCCCCCEEEEEECCCEEEEEEEEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCEEEEE
DTITAPEGILLVTGPTGSGKTTTLYSVLQELKGPLTNIMTIEDPPEYKLPGIAQIAVKPK
ECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHEEECCCCCCCCCCCEEEEEECCC
IGLTFARGLRHLLRQDPDILMVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRL
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHH
LDMGIESYLLSATLVGVVAQRLVRTICPYCKVAYTPENQEKSFLASLGKDTEMPLYRGQG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHCCCCCCCCCCCCCH
CVHCFRSGYKGRQGIYEFLRPNTLFRSEVASNRPYHILRETAEQNGFLPILEHGIALAVS
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCEEEEEC
GETTLAEVLRVTKRCD
CCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AASILSQELLDILPYTFLKKHCLLPIEESSEAITIAHATATSVIAQDEVKLLIKKPVRF
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
VLKEESEILQRLQQLYSNREGNVSDMLLTMKEEDGTTISEEEDLLETTDTIPVVRLLNWI
HHHHHHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHH
LKEAIEERASDIHFEPCEDSMRIRYRIDGVLHDRHSPPSHLRSALTTRLKVLAKMDIAEH
HHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RLPQDGRIKIHIGGQEVDMRVSTVPVIYGERVVLRILDKRNVILDIAGLHMPKGTEILFK
CCCCCCEEEEEECCCEEEEEEEEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCEEEEE
DTITAPEGILLVTGPTGSGKTTTLYSVLQELKGPLTNIMTIEDPPEYKLPGIAQIAVKPK
ECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHEEECCCCCCCCCCCEEEEEECCC
IGLTFARGLRHLLRQDPDILMVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRL
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHH
LDMGIESYLLSATLVGVVAQRLVRTICPYCKVAYTPENQEKSFLASLGKDTEMPLYRGQG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHCCCCCCCCCCCCCH
CVHCFRSGYKGRQGIYEFLRPNTLFRSEVASNRPYHILRETAEQNGFLPILEHGIALAVS
HHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCEEEEEC
GETTLAEVLRVTKRCD
CCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]