Click here to switch to the map view.

The map label for this gene is yohI

Identifier: 15836301

GI number: 15836301

Start: 863480

End: 864487

Strand: Direct

Name: yohI

Synonym: CPj0768

Alternate gene names: 15836301

Gene position: 863480-864487 (Clockwise)

Preceding gene: 15836300

Following gene: 15836303

Centisome position: 70.4

GC content: 43.45

Gene sequence:

>1008_bases
ATGGCTGCTCCAATATTTATAAAAAATATTTTACTTCGTTCCTCTATAGTCTATGCTCCTCTAGCGGGATTTTCAGATTA
TCCCTACCGTTGCATGTCCGCATTGTATCAACCAGGGTTGATGTTTTGTGAAATGGTGAAAGTAGAAGGGATACTCTACG
CTCCTGAGCGTACTTCGAAGCTTCTAGATTATAATGAGAACATGCGTCCCATAGGAGCGCAGTTGTGCGGTAGTAATCCA
GAAACTAGTGGGGAGGCCGCTAAAATTTTAGAAGGCCTTGGTTTCGACCTTATAGACCTAAATTGTGGATGTCCTACAGA
TAAAATCACCAAAGATGGCAGTGGGTCAGGTCTTTTGAAGACGCCAGAGCTTATTGGGAGGATTTTAGATAAAATCATCA
ATAGCGTTTCCATTCCTGTAACAGTAAAAATTCGCTCGGGTTGGGATATGGAACATATCAACGTAGAGGATACGGTACGT
ATTATACGTGATGCTGGAGCTAGCGCAGTTTTTGTTCACGGGAGAACTCGTGCTCAGGGATACCACGGTCCTAGCAAGCA
AGAGTATATTTCTAGAGCCAAGGCTGCTGCAGGAAAAGAATTCCCAGTTTTTGGTAACGGAGATATTTTTTCTCCAGAAG
CTGCGCAAGCAATGCTAACTACAGGATGTGATGGTGTTCTGGTAGCTCGAGGAACCTTGGGAGCCCCTTGGATTGGAAAA
CAAATCCAAGACTATCTCACTACAGGAAGCTATGAGAAAATTCCCTTTATCAAAAGGAAAGCTGCGTTTCTGGAGCATAT
GCGCCTAGTAGAAGACTATTATCAAAGCGAAACGAAGTTCCTTTCAGAAACACGTAAATTATGTGGCCACTACCTAATTT
CCGCGGCTAAGGTGCGTTTTCTTCGTTCGTCTCTAGCAAAAGCGACATCCTACCAAGAAGTCTACCAGCTTGTGAATGAT
TACGAAGAAGCCGACGACTCGTCATTAGAGACCTTTGTTAAATGCTGA

Upstream 100 bases:

>100_bases
TAGATCCCAGTCCTTTTGTTGGTCTGCTTTGCCTTGGAATCCTTCCTTTTGTTATATTAAGAGTCCTACGTTTTATTATT
CTTAATATTTTTCATTCTCC

Downstream 100 bases:

>100_bases
CTTAGGTGTTTCGAAAGTTGGAACATATCGATAGGATTCGGACCAATGATCGTAGCTAAATTGTTATCAGGAACTAAAAG
TTTTTTATTTTCTGGTGCCT

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAAPIFIKNILLRSSIVYAPLAGFSDYPYRCMSALYQPGLMFCEMVKVEGILYAPERTSKLLDYNENMRPIGAQLCGSNP
ETSGEAAKILEGLGFDLIDLNCGCPTDKITKDGSGSGLLKTPELIGRILDKIINSVSIPVTVKIRSGWDMEHINVEDTVR
IIRDAGASAVFVHGRTRAQGYHGPSKQEYISRAKAAAGKEFPVFGNGDIFSPEAAQAMLTTGCDGVLVARGTLGAPWIGK
QIQDYLTTGSYEKIPFIKRKAAFLEHMRLVEDYYQSETKFLSETRKLCGHYLISAAKVRFLRSSLAKATSYQEVYQLVND
YEEADDSSLETFVKC

Sequences:

>Translated_335_residues
MAAPIFIKNILLRSSIVYAPLAGFSDYPYRCMSALYQPGLMFCEMVKVEGILYAPERTSKLLDYNENMRPIGAQLCGSNP
ETSGEAAKILEGLGFDLIDLNCGCPTDKITKDGSGSGLLKTPELIGRILDKIINSVSIPVTVKIRSGWDMEHINVEDTVR
IIRDAGASAVFVHGRTRAQGYHGPSKQEYISRAKAAAGKEFPVFGNGDIFSPEAAQAMLTTGCDGVLVARGTLGAPWIGK
QIQDYLTTGSYEKIPFIKRKAAFLEHMRLVEDYYQSETKFLSETRKLCGHYLISAAKVRFLRSSLAKATSYQEVYQLVND
YEEADDSSLETFVKC
>Mature_334_residues
AAPIFIKNILLRSSIVYAPLAGFSDYPYRCMSALYQPGLMFCEMVKVEGILYAPERTSKLLDYNENMRPIGAQLCGSNPE
TSGEAAKILEGLGFDLIDLNCGCPTDKITKDGSGSGLLKTPELIGRILDKIINSVSIPVTVKIRSGWDMEHINVEDTVRI
IRDAGASAVFVHGRTRAQGYHGPSKQEYISRAKAAAGKEFPVFGNGDIFSPEAAQAMLTTGCDGVLVARGTLGAPWIGKQ
IQDYLTTGSYEKIPFIKRKAAFLEHMRLVEDYYQSETKFLSETRKLCGHYLISAAKVRFLRSSLAKATSYQEVYQLVNDY
EEADDSSLETFVKC

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=286, Percent_Identity=29.7202797202797, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI239788462, Length=294, Percent_Identity=29.5918367346939, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=30.8370044052863, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=29.2887029288703, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI8923374, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789660, Length=265, Percent_Identity=34.3396226415094, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788462, Length=313, Percent_Identity=29.073482428115, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI145693211, Length=231, Percent_Identity=29.8701298701299, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=296, Percent_Identity=26.3513513513513, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=283, Percent_Identity=25.7950530035336, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=27.9279279279279, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=323, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=196, Percent_Identity=34.6938775510204, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=236, Percent_Identity=26.271186440678, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585320, Length=289, Percent_Identity=29.757785467128, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24580595, Length=259, Percent_Identity=27.7992277992278, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19920448, Length=259, Percent_Identity=27.7992277992278, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=27.2321428571429, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36947; Mature: 36816

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPIFIKNILLRSSIVYAPLAGFSDYPYRCMSALYQPGLMFCEMVKVEGILYAPERTSK
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCHHHHH
LLDYNENMRPIGAQLCGSNPETSGEAAKILEGLGFDLIDLNCGCPTDKITKDGSGSGLLK
HHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCC
TPELIGRILDKIINSVSIPVTVKIRSGWDMEHINVEDTVRIIRDAGASAVFVHGRTRAQG
CHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHHHHHHHHCCCCEEEEECCHHCCC
YHGPSKQEYISRAKAAAGKEFPVFGNGDIFSPEAAQAMLTTGCDGVLVARGTLGAPWIGK
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHH
QIQDYLTTGSYEKIPFIKRKAAFLEHMRLVEDYYQSETKFLSETRKLCGHYLISAAKVRF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRSSLAKATSYQEVYQLVNDYEEADDSSLETFVKC
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
AAPIFIKNILLRSSIVYAPLAGFSDYPYRCMSALYQPGLMFCEMVKVEGILYAPERTSK
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCHHHHH
LLDYNENMRPIGAQLCGSNPETSGEAAKILEGLGFDLIDLNCGCPTDKITKDGSGSGLLK
HHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCC
TPELIGRILDKIINSVSIPVTVKIRSGWDMEHINVEDTVRIIRDAGASAVFVHGRTRAQG
CHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHHHHHHHHCCCCEEEEECCHHCCC
YHGPSKQEYISRAKAAAGKEFPVFGNGDIFSPEAAQAMLTTGCDGVLVARGTLGAPWIGK
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHH
QIQDYLTTGSYEKIPFIKRKAAFLEHMRLVEDYYQSETKFLSETRKLCGHYLISAAKVRF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRSSLAKATSYQEVYQLVNDYEEADDSSLETFVKC
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA