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The map label for this gene is dacF

Identifier: 15836204

GI number: 15836204

Start: 753063

End: 754373

Strand: Direct

Name: dacF

Synonym: CPj0672

Alternate gene names: 15836204

Gene position: 753063-754373 (Clockwise)

Preceding gene: 15836202

Following gene: 15836205

Centisome position: 61.4

GC content: 44.09

Gene sequence:

>1311_bases
ATGAAAAGACCTTTTTTTACCTATCTATGCATCATCTTCTACGGATCTTGTGCATCGTTATCTTTACATGCAGGACTCTC
TTTCCCAGAAGTACGTGGAGCTACGGCTGCTGTTGTCCATGCCGACTCTGGGAAGGTATTCTATGATAAAGACATAGATG
CTGTAATCTATCCTGCCAGCATGACGAAAATCGCAACTGCCCTCTTTATCCTAAAGCACTATCCCACAGTCCTCGATACT
CTCATCAAAGTCAAACAAGATGCGATCGCTTCCATCACTCCGCAAGCAAAAAAACAATCAGGATATCGTAGTCCTCCCCA
CTGGTTAGAAACTGATGGATCTACAATACAGCTCCATCTTCGAGAAGAGCTTTTAGGGTGGGACCTGTTCCACGCCTTAC
TGGTCTGTTCTGCTAATGATGCTGCGAATGTCTTAGCTATGGCATGTTGCGGATCTGTAGAGAAGTTTATGGATAAGCTG
AACTTCTTCTTAAAAGAAGAAATCGGCTGCACTCATACCCATTTTAATAATCCCCATGGGTTACATCATCCGAATCACTA
TACTACAACCCGTGATCTTATTAGCATCATGCGTTGCGCTCTGAAAGAACCTCCATTTCGAGGGGTCATCTCCACGACAA
GCTATAAAATAGGGGCTACAAACCTGCATGGCGAACGGATCCTATCCCCAACAAACAAATTGCTTCTTCCTGGGTCTACC
TACCACTATCCCCCAGCTTTAGGAGGGAAAACAGGGACCACCAAGACTGCAGGGAAAAATCTAATTATGGCTGCTGAAAA
AAATAACCGCCTCTTGGTAACGATCGCAACGGGCTATTCGGGTCCTGTGAGTGATCTCTACCAAGATGTCATTGCTCTAT
GTGAAACGGTATTTAACGAGCCGCTATTAAGAAAAGAGCTCGTCCCCCCCTCCGACTGTCTCCAATTAGAAATAGCGAAT
CTTGGGAAGCTTTCTTGCCCTCTTCCTGAGGGACTCTACTATGACTTCTATGCCTCCGAAGATCGCGAACCTCTTTCTGT
ATCTTTTATTGCACATGCGGACGCCTTCCCTATTGAACAAGGAGATCTTCTTGGTCATTGGGTTTTTTATGACGATGAAG
GCAAGAAAATTTCTTCCCAGCCTTTCTATGCCCCTTGTCGTTTTGAGCGCACTATCAAGCCTTGGAAACTCTATATGAAA
CGTGTCTTCACATCGTATAGAACCTATATGTCTATAACCATGCTGCTCATGTATTTTCGCATCCGCAAGCACCGCAAGTA
TAAAAATTTAAAACACTATTCTAAAATCTAA

Upstream 100 bases:

>100_bases
CTCTGATTTATGGTAATTCTTTATTTTCAGAGCCGTCAAGTCCTTTCTATTCTGTTGAATTTCCTAATAACGTAAGTAAT
AAACAATCAAAAGTCCGCAT

Downstream 100 bases:

>100_bases
CTTTTTCTTTTAATTTATAAAAAACCAAAGGTTTATGTAAGATTTGCGCTTTTCAATCCAACAAGAATCCCTTGTGCGCA
CATTACTTTGCTGTTTACTT

Product: D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKRPFFTYLCIIFYGSCASLSLHAGLSFPEVRGATAAVVHADSGKVFYDKDIDAVIYPASMTKIATALFILKHYPTVLDT
LIKVKQDAIASITPQAKKQSGYRSPPHWLETDGSTIQLHLREELLGWDLFHALLVCSANDAANVLAMACCGSVEKFMDKL
NFFLKEEIGCTHTHFNNPHGLHHPNHYTTTRDLISIMRCALKEPPFRGVISTTSYKIGATNLHGERILSPTNKLLLPGST
YHYPPALGGKTGTTKTAGKNLIMAAEKNNRLLVTIATGYSGPVSDLYQDVIALCETVFNEPLLRKELVPPSDCLQLEIAN
LGKLSCPLPEGLYYDFYASEDREPLSVSFIAHADAFPIEQGDLLGHWVFYDDEGKKISSQPFYAPCRFERTIKPWKLYMK
RVFTSYRTYMSITMLLMYFRIRKHRKYKNLKHYSKI

Sequences:

>Translated_436_residues
MKRPFFTYLCIIFYGSCASLSLHAGLSFPEVRGATAAVVHADSGKVFYDKDIDAVIYPASMTKIATALFILKHYPTVLDT
LIKVKQDAIASITPQAKKQSGYRSPPHWLETDGSTIQLHLREELLGWDLFHALLVCSANDAANVLAMACCGSVEKFMDKL
NFFLKEEIGCTHTHFNNPHGLHHPNHYTTTRDLISIMRCALKEPPFRGVISTTSYKIGATNLHGERILSPTNKLLLPGST
YHYPPALGGKTGTTKTAGKNLIMAAEKNNRLLVTIATGYSGPVSDLYQDVIALCETVFNEPLLRKELVPPSDCLQLEIAN
LGKLSCPLPEGLYYDFYASEDREPLSVSFIAHADAFPIEQGDLLGHWVFYDDEGKKISSQPFYAPCRFERTIKPWKLYMK
RVFTSYRTYMSITMLLMYFRIRKHRKYKNLKHYSKI
>Mature_436_residues
MKRPFFTYLCIIFYGSCASLSLHAGLSFPEVRGATAAVVHADSGKVFYDKDIDAVIYPASMTKIATALFILKHYPTVLDT
LIKVKQDAIASITPQAKKQSGYRSPPHWLETDGSTIQLHLREELLGWDLFHALLVCSANDAANVLAMACCGSVEKFMDKL
NFFLKEEIGCTHTHFNNPHGLHHPNHYTTTRDLISIMRCALKEPPFRGVISTTSYKIGATNLHGERILSPTNKLLLPGST
YHYPPALGGKTGTTKTAGKNLIMAAEKNNRLLVTIATGYSGPVSDLYQDVIALCETVFNEPLLRKELVPPSDCLQLEIAN
LGKLSCPLPEGLYYDFYASEDREPLSVSFIAHADAFPIEQGDLLGHWVFYDDEGKKISSQPFYAPCRFERTIKPWKLYMK
RVFTSYRTYMSITMLLMYFRIRKHRKYKNLKHYSKI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=245, Percent_Identity=28.9795918367347, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786851, Length=285, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082022, Length=254, Percent_Identity=25.5905511811024, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49152; Mature: 49152

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPFFTYLCIIFYGSCASLSLHAGLSFPEVRGATAAVVHADSGKVFYDKDIDAVIYPAS
CCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEEECCCCEEEECHH
MTKIATALFILKHYPTVLDTLIKVKQDAIASITPQAKKQSGYRSPPHWLETDGSTIQLHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEE
REELLGWDLFHALLVCSANDAANVLAMACCGSVEKFMDKLNFFLKEEIGCTHTHFNNPHG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LHHPNHYTTTRDLISIMRCALKEPPFRGVISTTSYKIGATNLHGERILSPTNKLLLPGST
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCHHCCCCCCEEECCCC
YHYPPALGGKTGTTKTAGKNLIMAAEKNNRLLVTIATGYSGPVSDLYQDVIALCETVFNE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
PLLRKELVPPSDCLQLEIANLGKLSCPLPEGLYYDFYASEDREPLSVSFIAHADAFPIEQ
CHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCC
GDLLGHWVFYDDEGKKISSQPFYAPCRFERTIKPWKLYMKRVFTSYRTYMSITMLLMYFR
CCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRKHRKYKNLKHYSKI
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRPFFTYLCIIFYGSCASLSLHAGLSFPEVRGATAAVVHADSGKVFYDKDIDAVIYPAS
CCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEEECCCCEEEECHH
MTKIATALFILKHYPTVLDTLIKVKQDAIASITPQAKKQSGYRSPPHWLETDGSTIQLHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEE
REELLGWDLFHALLVCSANDAANVLAMACCGSVEKFMDKLNFFLKEEIGCTHTHFNNPHG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LHHPNHYTTTRDLISIMRCALKEPPFRGVISTTSYKIGATNLHGERILSPTNKLLLPGST
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCHHCCCCCCEEECCCC
YHYPPALGGKTGTTKTAGKNLIMAAEKNNRLLVTIATGYSGPVSDLYQDVIALCETVFNE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
PLLRKELVPPSDCLQLEIANLGKLSCPLPEGLYYDFYASEDREPLSVSFIAHADAFPIEQ
CHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCC
GDLLGHWVFYDDEGKKISSQPFYAPCRFERTIKPWKLYMKRVFTSYRTYMSITMLLMYFR
CCEEEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRKHRKYKNLKHYSKI
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]