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The map label for this gene is truA

Identifier: 15836112

GI number: 15836112

Start: 669314

End: 670117

Strand: Direct

Name: truA

Synonym: CPj0580

Alternate gene names: 15836112

Gene position: 669314-670117 (Clockwise)

Preceding gene: 15836111

Following gene: 15836114

Centisome position: 54.57

GC content: 44.15

Gene sequence:

>804_bases
ATGACTAAAGTAGCTCTTCTTATTGCTTATCAAGGAACTGCCTATTCAGGCTGGCAACAACAACCGAATGACCTATCGAT
TCAGGAGGTTATTGAAAGTTCCCTAAAGAAAATTACTAAAACTCGCACTCCACTAATTGCCTCTGGGAGAACCGACGCAG
GCGTCCATGCCTACGGGCAAGTGGCGCATTTCCGAGCTCCTGATCACCCTCTATTTGCAAACGCGAACCTTACAAAAAAA
GCCCTCAATGCGATTCTCCCTAAAGATATTGTAATCAGAGATGTTGCTTTGTTTGATGATAATTTCCATGCACGCTATCT
TACCATTGCTAAAGAATATCGTTATTCCCTATCAAGACTTGCCAAACCTCTTCCCTGGCAGCGCCATTTCTGTTATACCC
CTCGCCACCCTTTTTCTACAGAGCTCATGCAGGAAGGTGCGAACCTGCTTATAGGAACTCATGACTTTGCCTCTTTTGCA
AATCATGGCAGGGACTATAACTCTACAGTACGAACGATCTATACCCTGGATATTGTAGATAAAGGAGATTCTCTCTCCAT
AATATGCAGAGGAAATGGCTTCCTTTATAAGATGGTACGGAATCTTGTAGGAGCCCTTTTAGATGTGGGGAAAGGAGCGT
ATCCACCTGAACATCTCCTAGATATCTTAGAACAGAAAAATCGTAGAGAAGGACCTTCGGCGGCTCCTGCCTACGGCCTT
TCTTTACACCACGTATGCTATTCCTCTCCCTACAATAACTTCTGTTGTGAGCAATGCTCTGTTAGCACGTCAAACGAAGG
ATAA

Upstream 100 bases:

>100_bases
CTGCTGAAATCATAGGCAAACCCTCGCAACTCGTTTTCAATAAGCATCCTCAAATCAAAATTTCCTACCCCGAAGATCTA
ACGATTGCCCAAGCCCTCCT

Downstream 100 bases:

>100_bases
GAGAAAAATTCTTTGCCTTTAAGTTCAGGGTAATCTTCAGGGGTGATCTCCGCCATCGAATTGATACATACTAAAGTTGC
TGGAATCTTGGAGAGCGCCC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTKVALLIAYQGTAYSGWQQQPNDLSIQEVIESSLKKITKTRTPLIASGRTDAGVHAYGQVAHFRAPDHPLFANANLTKK
ALNAILPKDIVIRDVALFDDNFHARYLTIAKEYRYSLSRLAKPLPWQRHFCYTPRHPFSTELMQEGANLLIGTHDFASFA
NHGRDYNSTVRTIYTLDIVDKGDSLSIICRGNGFLYKMVRNLVGALLDVGKGAYPPEHLLDILEQKNRREGPSAAPAYGL
SLHHVCYSSPYNNFCCEQCSVSTSNEG

Sequences:

>Translated_267_residues
MTKVALLIAYQGTAYSGWQQQPNDLSIQEVIESSLKKITKTRTPLIASGRTDAGVHAYGQVAHFRAPDHPLFANANLTKK
ALNAILPKDIVIRDVALFDDNFHARYLTIAKEYRYSLSRLAKPLPWQRHFCYTPRHPFSTELMQEGANLLIGTHDFASFA
NHGRDYNSTVRTIYTLDIVDKGDSLSIICRGNGFLYKMVRNLVGALLDVGKGAYPPEHLLDILEQKNRREGPSAAPAYGL
SLHHVCYSSPYNNFCCEQCSVSTSNEG
>Mature_266_residues
TKVALLIAYQGTAYSGWQQQPNDLSIQEVIESSLKKITKTRTPLIASGRTDAGVHAYGQVAHFRAPDHPLFANANLTKKA
LNAILPKDIVIRDVALFDDNFHARYLTIAKEYRYSLSRLAKPLPWQRHFCYTPRHPFSTELMQEGANLLIGTHDFASFAN
HGRDYNSTVRTIYTLDIVDKGDSLSIICRGNGFLYKMVRNLVGALLDVGKGAYPPEHLLDILEQKNRREGPSAAPAYGLS
LHHVCYSSPYNNFCCEQCSVSTSNEG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=289, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI199559785, Length=291, Percent_Identity=28.1786941580756, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=34.251968503937, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CHLPN (Q9Z7X4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72062
- PIR:   A86563
- PIR:   E81605
- RefSeq:   NP_224776.1
- RefSeq:   NP_300636.1
- RefSeq:   NP_444720.1
- RefSeq:   NP_876876.1
- ProteinModelPortal:   Q9Z7X4
- SMR:   Q9Z7X4
- GeneID:   1467283
- GeneID:   895457
- GeneID:   919338
- GeneID:   962976
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0168
- KEGG:   cpn:CPn0580
- KEGG:   cpt:CpB0605
- TIGR:   CP_0168
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q9Z7X4
- ProtClustDB:   PRK00021
- BioCyc:   CPNE115711:CP_0168-MONOMER
- BioCyc:   CPNE115713:CPN0580-MONOMER
- BioCyc:   CPNE138677:CPJ0580-MONOMER
- BioCyc:   CPNE182082:CPB0605-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29819; Mature: 29688

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVALLIAYQGTAYSGWQQQPNDLSIQEVIESSLKKITKTRTPLIASGRTDAGVHAYGQ
CCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHH
VAHFRAPDHPLFANANLTKKALNAILPKDIVIRDVALFDDNFHARYLTIAKEYRYSLSRL
HHCCCCCCCCEEECCCHHHHHHHHHCCCHHHHEEHEEECCCCCCHHHHHHHHHHHHHHHH
AKPLPWQRHFCYTPRHPFSTELMQEGANLLIGTHDFASFANHGRDYNSTVRTIYTLDIVD
CCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCEEEEEEEEEEE
KGDSLSIICRGNGFLYKMVRNLVGALLDVGKGAYPPEHLLDILEQKNRREGPSAAPAYGL
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
SLHHVCYSSPYNNFCCEQCSVSTSNEG
CHHHHHHCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
TKVALLIAYQGTAYSGWQQQPNDLSIQEVIESSLKKITKTRTPLIASGRTDAGVHAYGQ
CCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHH
VAHFRAPDHPLFANANLTKKALNAILPKDIVIRDVALFDDNFHARYLTIAKEYRYSLSRL
HHCCCCCCCCEEECCCHHHHHHHHHCCCHHHHEEHEEECCCCCCHHHHHHHHHHHHHHHH
AKPLPWQRHFCYTPRHPFSTELMQEGANLLIGTHDFASFANHGRDYNSTVRTIYTLDIVD
CCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCEEEEEEEEEEE
KGDSLSIICRGNGFLYKMVRNLVGALLDVGKGAYPPEHLLDILEQKNRREGPSAAPAYGL
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
SLHHVCYSSPYNNFCCEQCSVSTSNEG
CHHHHHHCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362