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The map label for this gene is ispD

Identifier: 15836111

GI number: 15836111

Start: 668682

End: 669317

Strand: Direct

Name: ispD

Synonym: CPj0579

Alternate gene names: 15836111

Gene position: 668682-669317 (Clockwise)

Preceding gene: 15836110

Following gene: 15836112

Centisome position: 54.52

GC content: 43.24

Gene sequence:

>636_bases
ATGATTAAGTCTTCTCTAATACTTCTTAGTGGAGGACAAGGTACACGTTTTGGATCTAAAATTCCTAAGCAGTACCTCCC
TCTAAATGGAACTCCCTTAGTTCTTCACTCATTAAAGATACTCTCTTCTTTGCCACAAATTGCTGAGGTGATTGTTGTTT
GCGACCCCTCATATCAAGAAACCTTTCAAGAATATCCTGTCTCTTTTGCCATTCCTGGAGAGCGTCGCCAAGATTCTGTC
TTTTCAGGACTACAGCAAGTCTCCTATCCCTGGGTAATCATCCACGATGGAGCACGTCCTTTTATCTATCCCGACGAAAT
TCATGATTTATTAGAAACAGCAGAAAAGATCGGGGCGACAGCTCTAGCGTCTCCGATTCCCTATACCATAAAACAACGCA
ATCCTGTGCGCACTCTGGACCGAGACAATTTAGCAATAATTCATACCCCTCAGTGTATAAAAACGGAAATCCTCAGAGAG
GGTCTAGCTCTTGCAAAAGAAAAACAGCTCACACTGGTAGACGACATCGAAGCTGCTGAAATCATAGGCAAACCCTCGCA
ACTCGTTTTCAATAAGCATCCTCAAATCAAAATTTCCTACCCCGAAGATCTAACGATTGCCCAAGCCCTCCTATGA

Upstream 100 bases:

>100_bases
ACTAAGGAAGGAAAACAACTCTACGTAAACCGCGGTCTCGGCGGACTAAAAAGAATTCGCTTCTGCTCCCCTCCTGAAAT
CTGCTACATCACATGTTCCT

Downstream 100 bases:

>100_bases
CTAAAGTAGCTCTTCTTATTGCTTATCAAGGAACTGCCTATTCAGGCTGGCAACAACAACCGAATGACCTATCGATTCAG
GAGGTTATTGAAAGTTCCCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MIKSSLILLSGGQGTRFGSKIPKQYLPLNGTPLVLHSLKILSSLPQIAEVIVVCDPSYQETFQEYPVSFAIPGERRQDSV
FSGLQQVSYPWVIIHDGARPFIYPDEIHDLLETAEKIGATALASPIPYTIKQRNPVRTLDRDNLAIIHTPQCIKTEILRE
GLALAKEKQLTLVDDIEAAEIIGKPSQLVFNKHPQIKISYPEDLTIAQALL

Sequences:

>Translated_211_residues
MIKSSLILLSGGQGTRFGSKIPKQYLPLNGTPLVLHSLKILSSLPQIAEVIVVCDPSYQETFQEYPVSFAIPGERRQDSV
FSGLQQVSYPWVIIHDGARPFIYPDEIHDLLETAEKIGATALASPIPYTIKQRNPVRTLDRDNLAIIHTPQCIKTEILRE
GLALAKEKQLTLVDDIEAAEIIGKPSQLVFNKHPQIKISYPEDLTIAQALL
>Mature_211_residues
MIKSSLILLSGGQGTRFGSKIPKQYLPLNGTPLVLHSLKILSSLPQIAEVIVVCDPSYQETFQEYPVSFAIPGERRQDSV
FSGLQQVSYPWVIIHDGARPFIYPDEIHDLLETAEKIGATALASPIPYTIKQRNPVRTLDRDNLAIIHTPQCIKTEILRE
GLALAKEKQLTLVDDIEAAEIIGKPSQLVFNKHPQIKISYPEDLTIAQALL

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=216, Percent_Identity=31.4814814814815, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CHLPN (Q9Z7X5)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   H72061
- PIR:   H86562
- RefSeq:   NP_224775.1
- RefSeq:   NP_300635.1
- RefSeq:   NP_444721.1
- RefSeq:   NP_876875.1
- ProteinModelPortal:   Q9Z7X5
- SMR:   Q9Z7X5
- GeneID:   1467282
- GeneID:   895471
- GeneID:   919340
- GeneID:   963833
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0169
- KEGG:   cpn:CPn0579
- KEGG:   cpt:CpB0603
- TIGR:   CP_0169
- HOGENOM:   HBG672839
- OMA:   KQMLEIG
- PhylomeDB:   Q9Z7X5
- ProtClustDB:   PRK00155
- BioCyc:   CPNE115711:CP_0169-MONOMER
- BioCyc:   CPNE115713:CPN0579-MONOMER
- BioCyc:   CPNE138677:CPJ0579-MONOMER
- BioCyc:   CPNE182082:CPB0603-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23447; Mature: 23447

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSSLILLSGGQGTRFGSKIPKQYLPLNGTPLVLHSLKILSSLPQIAEVIVVCDPSYQE
CCCCCEEEEECCCCCCCHHHCCHHHCCCCCCHHHHHHHHHHHHCHHHHEEEEEECCCHHH
TFQEYPVSFAIPGERRQDSVFSGLQQVSYPWVIIHDGARPFIYPDEIHDLLETAEKIGAT
HHHHCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCHH
ALASPIPYTIKQRNPVRTLDRDNLAIIHTPQCIKTEILREGLALAKEKQLTLVDDIEAAE
HHCCCCCEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
IIGKPSQLVFNKHPQIKISYPEDLTIAQALL
HHCCCHHHEECCCCCEEEECCCCCHHHHHCC
>Mature Secondary Structure
MIKSSLILLSGGQGTRFGSKIPKQYLPLNGTPLVLHSLKILSSLPQIAEVIVVCDPSYQE
CCCCCEEEEECCCCCCCHHHCCHHHCCCCCCHHHHHHHHHHHHCHHHHEEEEEECCCHHH
TFQEYPVSFAIPGERRQDSVFSGLQQVSYPWVIIHDGARPFIYPDEIHDLLETAEKIGAT
HHHHCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCHH
ALASPIPYTIKQRNPVRTLDRDNLAIIHTPQCIKTEILREGLALAKEKQLTLVDDIEAAE
HHCCCCCEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
IIGKPSQLVFNKHPQIKISYPEDLTIAQALL
HHCCCHHHEECCCCCEEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362