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The map label for this gene is cysJ

Identifier: 15836079

GI number: 15836079

Start: 631508

End: 632551

Strand: Reverse

Name: cysJ

Synonym: CPj0548

Alternate gene names: 15836079

Gene position: 632551-631508 (Counterclockwise)

Preceding gene: 15836080

Following gene: 15836077

Centisome position: 51.57

GC content: 40.13

Gene sequence:

>1044_bases
ATGTACCTACAAGAAAAGTTTAAGGCTCAGCAAGTTCCTCTAGTATTGCGTGAGCTACTTTCTTGTTCCGATTCCATTAA
CGATAGCGATCCCATCTACCGCATGGTCTTTGATAGCAATGATACGACTATATCCTATAAAGTAGGCGATGCTTTAGGAG
TCTTACCAGAGAATTCTAAAGAGGTCTCCGAACACGTTCTCCAGCTTCTAGGCTATTCCCCAACGACCCTTGTCAACGTA
AAAAAAACTTCTGAAAAAGTTTCTGCCCAAAAATTTATCCAAGGCTATGTTGATCTTGATAAAATTCCAGCAAAGCTAAA
TTCCTTTTTCCCAGACAAAGATCCTAAGATTACACTGTATGATGCCATTCAAGAGTACCGCCCTCAGATCCCAATAGAAC
TCTTCGCTGAGAGTGTGTTTCCTTTATTGCCTCGTTTCTACTCTATAGCCTCCTCACCAGATCTACATCCCAAAAGTATA
GAGCTATTGGTAAAACACGTTTCCTATCCTGGAAAATATCAAAAACGCTTCGGAGTTTGCTCGTCCTTTCTATGTAGCGA
ACTACAGGTCAACGATTCTGCCTACATATTCGTACAACCTACAAAACACTTCACTCTATCTACACAGACTGAAGGGAAAC
CCTTAGTTATGATCGGAGCTGGAACAGGAATCGCTCCTTATAAAGCCTTCCTAGAGGAACGCCTTTTCAATAAAGACCCA
GGAAATAATTTACTATTTTTTGGTGAGCGCAAAGAAAAAGTGAACTTCTATTATCGAGAATTCTGGAACCATGCAGAAGA
GGAAGGAAAACTAAAACTCTTTTTAGCCTTCTCAAGAGAGAGAGACCAAAAAGTCTATGTTCAAGATCTTCTTAGGATTC
AAAAAGATGAGGTAAGAAAGGCCTATGAAGAGGGAGGGTTTTTCTTTGTCTGTGGACGTAAAGTCTTAGGCATAGAAGTA
AAACACGCTTTAGAAGAAATCTTAGGAAAAGATACTCTAGCCTCCCTCAGAAAGGAACATCGTTACGTTGTTGACGTCTA
TTAG

Upstream 100 bases:

>100_bases
ATCCCACAGGAAAAACTATCGATGCTTTGAAAATGTTAGCACTTCCTGCAGGAGTTGATATTAAAATCAAAGCGGCTTAA
TTCAAGCTTGTCTATAAAAG

Downstream 100 bases:

>100_bases
TCACAGTATTCCATCACAGTTAAGACGCAGAAACACTGAACGCCATCTCCACAGCCAAAGTCACTCAAACCCTCTCCAGA
AGTCGCAGTAATGCCAATAT

Product: sulfite reductase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MYLQEKFKAQQVPLVLRELLSCSDSINDSDPIYRMVFDSNDTTISYKVGDALGVLPENSKEVSEHVLQLLGYSPTTLVNV
KKTSEKVSAQKFIQGYVDLDKIPAKLNSFFPDKDPKITLYDAIQEYRPQIPIELFAESVFPLLPRFYSIASSPDLHPKSI
ELLVKHVSYPGKYQKRFGVCSSFLCSELQVNDSAYIFVQPTKHFTLSTQTEGKPLVMIGAGTGIAPYKAFLEERLFNKDP
GNNLLFFGERKEKVNFYYREFWNHAEEEGKLKLFLAFSRERDQKVYVQDLLRIQKDEVRKAYEEGGFFFVCGRKVLGIEV
KHALEEILGKDTLASLRKEHRYVVDVY

Sequences:

>Translated_347_residues
MYLQEKFKAQQVPLVLRELLSCSDSINDSDPIYRMVFDSNDTTISYKVGDALGVLPENSKEVSEHVLQLLGYSPTTLVNV
KKTSEKVSAQKFIQGYVDLDKIPAKLNSFFPDKDPKITLYDAIQEYRPQIPIELFAESVFPLLPRFYSIASSPDLHPKSI
ELLVKHVSYPGKYQKRFGVCSSFLCSELQVNDSAYIFVQPTKHFTLSTQTEGKPLVMIGAGTGIAPYKAFLEERLFNKDP
GNNLLFFGERKEKVNFYYREFWNHAEEEGKLKLFLAFSRERDQKVYVQDLLRIQKDEVRKAYEEGGFFFVCGRKVLGIEV
KHALEEILGKDTLASLRKEHRYVVDVY
>Mature_347_residues
MYLQEKFKAQQVPLVLRELLSCSDSINDSDPIYRMVFDSNDTTISYKVGDALGVLPENSKEVSEHVLQLLGYSPTTLVNV
KKTSEKVSAQKFIQGYVDLDKIPAKLNSFFPDKDPKITLYDAIQEYRPQIPIELFAESVFPLLPRFYSIASSPDLHPKSI
ELLVKHVSYPGKYQKRFGVCSSFLCSELQVNDSAYIFVQPTKHFTLSTQTEGKPLVMIGAGTGIAPYKAFLEERLFNKDP
GNNLLFFGERKEKVNFYYREFWNHAEEEGKLKLFLAFSRERDQKVYVQDLLRIQKDEVRKAYEEGGFFFVCGRKVLGIEV
KHALEEILGKDTLASLRKEHRYVVDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI10835173, Length=385, Percent_Identity=27.5324675324675, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI7657393, Length=369, Percent_Identity=26.8292682926829, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI221316707, Length=302, Percent_Identity=28.1456953642384, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI221316705, Length=378, Percent_Identity=25.9259259259259, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI221316709, Length=369, Percent_Identity=26.2872628726287, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI127139033, Length=380, Percent_Identity=25.7894736842105, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40254422, Length=225, Percent_Identity=32.8888888888889, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI24041029, Length=236, Percent_Identity=33.4745762711864, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI169790958, Length=427, Percent_Identity=27.4004683840749, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI169790956, Length=427, Percent_Identity=27.1662763466042, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789123, Length=373, Percent_Identity=31.0991957104558, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17554134, Length=387, Percent_Identity=26.6149870801034, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17566446, Length=346, Percent_Identity=26.878612716763, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321143, Length=369, Percent_Identity=27.9132791327913, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321832, Length=421, Percent_Identity=24.9406175771971, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325305, Length=302, Percent_Identity=23.1788079470199, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583543, Length=374, Percent_Identity=29.6791443850267, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI78706876, Length=374, Percent_Identity=29.6791443850267, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI78706872, Length=374, Percent_Identity=29.6791443850267, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24582192, Length=371, Percent_Identity=26.9541778975741, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137192, Length=371, Percent_Identity=26.9541778975741, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24660907, Length=366, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24660903, Length=366, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 39890; Mature: 39890

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLQEKFKAQQVPLVLRELLSCSDSINDSDPIYRMVFDSNDTTISYKVGDALGVLPENSK
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEECCEEECCCCCHH
EVSEHVLQLLGYSPTTLVNVKKTSEKVSAQKFIQGYVDLDKIPAKLNSFFPDKDPKITLY
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEHH
DAIQEYRPQIPIELFAESVFPLLPRFYSIASSPDLHPKSIELLVKHVSYPGKYQKRFGVC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
SSFLCSELQVNDSAYIFVQPTKHFTLSTQTEGKPLVMIGAGTGIAPYKAFLEERLFNKDP
HHHHHHHHEECCCEEEEEECCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
GNNLLFFGERKEKVNFYYREFWNHAEEEGKLKLFLAFSRERDQKVYVQDLLRIQKDEVRK
CCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
AYEEGGFFFVCGRKVLGIEVKHALEEILGKDTLASLRKEHRYVVDVY
HHHCCCEEEEECCEEEHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MYLQEKFKAQQVPLVLRELLSCSDSINDSDPIYRMVFDSNDTTISYKVGDALGVLPENSK
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEECCEEECCCCCHH
EVSEHVLQLLGYSPTTLVNVKKTSEKVSAQKFIQGYVDLDKIPAKLNSFFPDKDPKITLY
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEHH
DAIQEYRPQIPIELFAESVFPLLPRFYSIASSPDLHPKSIELLVKHVSYPGKYQKRFGVC
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
SSFLCSELQVNDSAYIFVQPTKHFTLSTQTEGKPLVMIGAGTGIAPYKAFLEERLFNKDP
HHHHHHHHEECCCEEEEEECCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
GNNLLFFGERKEKVNFYYREFWNHAEEEGKLKLFLAFSRERDQKVYVQDLLRIQKDEVRK
CCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
AYEEGGFFFVCGRKVLGIEVKHALEEILGKDTLASLRKEHRYVVDVY
HHHCCCEEEEECCEEEHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA