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The map label for this gene is ispF

Identifier: 15836078

GI number: 15836078

Start: 630981

End: 631511

Strand: Direct

Name: ispF

Synonym: CPj0547

Alternate gene names: 15836078

Gene position: 630981-631511 (Clockwise)

Preceding gene: 15836074

Following gene: 15836085

Centisome position: 51.44

GC content: 41.81

Gene sequence:

>531_bases
ATGGATAGAGACAATGAGGTTCCCCTGCCCAAGCCCAAATGGATTTACCGTACAGGTATTGGTCAAGACAGCCACCGCTT
TCTCCCAGAAAGTTCCACGAAGCCCTGCATTTTAGGTGGTATCATTTTTGATCATTGCCCAGGATTTCAGGCAAATTCCG
ATGGAGACATTATCTTTCATGCCATTTGTAATGCGATTTCCTCAGTAACTAATAAAATTATTTTAGGAAAGGTTGCTGAT
GAGCTTCTCCAAACACGGGGAATTACAGATAGTGGGATTTATCTTGAGGAGGCTTTAAAATCTCTGAAGCCTAATCAAAA
GATTTCTCATGTCGCTATTACGATTGAAGGAAGTCGACCTAAATTTCTCTGTAAGCTATCTGCATTACGTCAAAATATTG
CCCAGGTTATGAACTTAACACCTACGGATATTGGCATTACTGCGACTTCTGGAGAGGGTTTGAGTGACTTTGGCTGTGGA
GATGGCGTTCAGTGTTTCTGCGTCTTAACTGTGATGGAATACTGTGACTAA

Upstream 100 bases:

>100_bases
GAGAAAGTTTGACAAATTACTGGAATAATGCGTGAATTTTAGAAATTTTGTAGTATCTTCTGTGAAAGAAATTCTTAAAA
AAAACATATACCAGGTAGTT

Downstream 100 bases:

>100_bases
TAGACGTCAACAACGTAACGATGTTCCTTTCTGAGGGAGGCTAGAGTATCTTTTCCTAAGATTTCTTCTAAAGCGTGTTT
TACTTCTATGCCTAAGACTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDRDNEVPLPKPKWIYRTGIGQDSHRFLPESSTKPCILGGIIFDHCPGFQANSDGDIIFHAICNAISSVTNKIILGKVAD
ELLQTRGITDSGIYLEEALKSLKPNQKISHVAITIEGSRPKFLCKLSALRQNIAQVMNLTPTDIGITATSGEGLSDFGCG
DGVQCFCVLTVMEYCD

Sequences:

>Translated_176_residues
MDRDNEVPLPKPKWIYRTGIGQDSHRFLPESSTKPCILGGIIFDHCPGFQANSDGDIIFHAICNAISSVTNKIILGKVAD
ELLQTRGITDSGIYLEEALKSLKPNQKISHVAITIEGSRPKFLCKLSALRQNIAQVMNLTPTDIGITATSGEGLSDFGCG
DGVQCFCVLTVMEYCD
>Mature_176_residues
MDRDNEVPLPKPKWIYRTGIGQDSHRFLPESSTKPCILGGIIFDHCPGFQANSDGDIIFHAICNAISSVTNKIILGKVAD
ELLQTRGITDSGIYLEEALKSLKPNQKISHVAITIEGSRPKFLCKLSALRQNIAQVMNLTPTDIGITATSGEGLSDFGCG
DGVQCFCVLTVMEYCD

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CHLPN (Q9Z805)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D72065
- PIR:   G86558
- RefSeq:   NP_224743.1
- RefSeq:   NP_300602.1
- RefSeq:   NP_444756.1
- RefSeq:   NP_876840.2
- ProteinModelPortal:   Q9Z805
- SMR:   Q9Z805
- GeneID:   1467247
- GeneID:   895434
- GeneID:   919308
- GeneID:   963831
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0205
- KEGG:   cpn:CPn0547
- KEGG:   cpt:CpB0568
- TIGR:   CP_0205
- HOGENOM:   HBG630070
- OMA:   VTHRIIL
- ProtClustDB:   PRK00084
- BioCyc:   CPNE115711:CP_0205-MONOMER
- BioCyc:   CPNE115713:CPN0547-MONOMER
- BioCyc:   CPNE138677:CPJ0547-MONOMER
- BioCyc:   CPNE182082:CPB0568-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 19131; Mature: 19131

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRDNEVPLPKPKWIYRTGIGQDSHRFLPESSTKPCILGGIIFDHCPGFQANSDGDIIFH
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCHHH
AICNAISSVTNKIILGKVADELLQTRGITDSGIYLEEALKSLKPNQKISHVAITIEGSRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCC
KFLCKLSALRQNIAQVMNLTPTDIGITATSGEGLSDFGCGDGVQCFCVLTVMEYCD
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRDNEVPLPKPKWIYRTGIGQDSHRFLPESSTKPCILGGIIFDHCPGFQANSDGDIIFH
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCHHH
AICNAISSVTNKIILGKVADELLQTRGITDSGIYLEEALKSLKPNQKISHVAITIEGSRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCC
KFLCKLSALRQNIAQVMNLTPTDIGITATSGEGLSDFGCGDGVQCFCVLTVMEYCD
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362