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The map label for this gene is dapF

Identifier: 15836050

GI number: 15836050

Start: 600221

End: 600994

Strand: Reverse

Name: dapF

Synonym: CPj0519

Alternate gene names: 15836050

Gene position: 600994-600221 (Counterclockwise)

Preceding gene: 15836051

Following gene: 15836049

Centisome position: 49.0

GC content: 43.67

Gene sequence:

>774_bases
ATGGCATTTTATTCTCCTTCAACGATCTCTAAATATTTTATCTATTCTGGAGCAGGAAATCGTTTCCTTCTTGGTGAAAC
ACTTCCTGAGGTTGAAGATGTTCGGTTCTTATGCCAAGAGACGAGGGTTGATGGTTTTTTATATTTAAAGCCCTCTTCTT
GTGCTGATGCGCAACTCATTATTTTTAATTCCGATGGATCACGTCCAACGATGTGTGGTAACGGCTTGCGTTGTGCGATT
GCTCACTTAGCTTCTCAGAAGGGAAAATCGGACATCTCTGTATCTACGGATAGTGGTCTATATTCAGGATATTTTTATTC
TTGGGATCGTGTGCTTGTAGATATGACTCTCGCAGATTGGAGAGCTTCTGTTCATCGATTGGAGTCGCGTCCTGATCCTC
TTCCCAAAGAGGTCGTTTGTATCCATACGGGAGTGCCTCATGCTGTCGTAATTCTTCCTGAGATTTCTACTTTAGATCTT
TCTATCTTAGGTCCTTTTCTTCGCTATCATCAGACCTTCTCTCCAGATGGGGTGAATGTCAATTTTGTTCAGATACTGGG
ACATTGCCAGTTGCGCGTTCGTACTTACGAACGTGGAGTCGAAGGGGAAACTGCAGCTTGTGGAACAGGGGCTCTAGCTT
CTGCTCTTGTTGTGTCAAACTCCTATGGATGGAAGGAGTCGATCCAAATCCATACTTGGGGTGGAGAGCTTATGACTGTG
AGTCAAAATAGGGGACGGGTATATCTTCAGGGCTCTGTAACTAGAGATTTATAA

Upstream 100 bases:

>100_bases
TATGTAGAGGCGACAAATCAACCTCGAGATATCATAGAAAAGGCTATCGATAGAGATATGTGGATGACAGCCAACGAAGC
TAAGGATTTTGGTTTATTGG

Downstream 100 bases:

>100_bases
TTAGATGTGATTTTTGATTTTGTCATGCAAGGATTTTAAAATCTTGTTTAGGGATAGATCTTGCTCTCTAACTGGGATTT
TTCTATAATCGTAATTTATG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAFYSPSTISKYFIYSGAGNRFLLGETLPEVEDVRFLCQETRVDGFLYLKPSSCADAQLIIFNSDGSRPTMCGNGLRCAI
AHLASQKGKSDISVSTDSGLYSGYFYSWDRVLVDMTLADWRASVHRLESRPDPLPKEVVCIHTGVPHAVVILPEISTLDL
SILGPFLRYHQTFSPDGVNVNFVQILGHCQLRVRTYERGVEGETAACGTGALASALVVSNSYGWKESIQIHTWGGELMTV
SQNRGRVYLQGSVTRDL

Sequences:

>Translated_257_residues
MAFYSPSTISKYFIYSGAGNRFLLGETLPEVEDVRFLCQETRVDGFLYLKPSSCADAQLIIFNSDGSRPTMCGNGLRCAI
AHLASQKGKSDISVSTDSGLYSGYFYSWDRVLVDMTLADWRASVHRLESRPDPLPKEVVCIHTGVPHAVVILPEISTLDL
SILGPFLRYHQTFSPDGVNVNFVQILGHCQLRVRTYERGVEGETAACGTGALASALVVSNSYGWKESIQIHTWGGELMTV
SQNRGRVYLQGSVTRDL
>Mature_256_residues
AFYSPSTISKYFIYSGAGNRFLLGETLPEVEDVRFLCQETRVDGFLYLKPSSCADAQLIIFNSDGSRPTMCGNGLRCAIA
HLASQKGKSDISVSTDSGLYSGYFYSWDRVLVDMTLADWRASVHRLESRPDPLPKEVVCIHTGVPHAVVILPEISTLDLS
ILGPFLRYHQTFSPDGVNVNFVQILGHCQLRVRTYERGVEGETAACGTGALASALVVSNSYGWKESIQIHTWGGELMTVS
QNRGRVYLQGSVTRDL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=194, Percent_Identity=32.9896907216495, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CHLPN (Q9Z833)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86555
- PIR:   F72067
- RefSeq:   NP_224715.1
- RefSeq:   NP_300574.1
- RefSeq:   NP_444785.1
- RefSeq:   NP_876812.1
- ProteinModelPortal:   Q9Z833
- SMR:   Q9Z833
- GeneID:   1467219
- GeneID:   895298
- GeneID:   919276
- GeneID:   963818
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0234
- KEGG:   cpn:CPn0519
- KEGG:   cpt:CpB0540
- TIGR:   CP_0234
- HOGENOM:   HBG399442
- OMA:   NCASVIA
- ProtClustDB:   PRK00450
- BioCyc:   CPNE115711:CP_0234-MONOMER
- BioCyc:   CPNE115713:CPN0519-MONOMER
- BioCyc:   CPNE138677:CPJ0519-MONOMER
- BioCyc:   CPNE182082:CPB0540-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 28323; Mature: 28192

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFYSPSTISKYFIYSGAGNRFLLGETLPEVEDVRFLCQETRVDGFLYLKPSSCADAQLI
CCEECCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
IFNSDGSRPTMCGNGLRCAIAHLASQKGKSDISVSTDSGLYSGYFYSWDRVLVDMTLADW
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEECEEEEECEEEEEEEHHHH
RASVHRLESRPDPLPKEVVCIHTGVPHAVVILPEISTLDLSILGPFLRYHQTFSPDGVNV
HHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCEEHHHHHHHHHHHHCCCCCCCCCE
NFVQILGHCQLRVRTYERGVEGETAACGTGALASALVVSNSYGWKESIQIHTWGGELMTV
EEEEEEECEEEEEEEECCCCCCCCCCCCCHHHHHEEEEECCCCCCEEEEEEEECCEEEEE
SQNRGRVYLQGSVTRDL
ECCCCEEEEEECCCCCC
>Mature Secondary Structure 
AFYSPSTISKYFIYSGAGNRFLLGETLPEVEDVRFLCQETRVDGFLYLKPSSCADAQLI
CEECCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
IFNSDGSRPTMCGNGLRCAIAHLASQKGKSDISVSTDSGLYSGYFYSWDRVLVDMTLADW
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEECEEEEECEEEEEEEHHHH
RASVHRLESRPDPLPKEVVCIHTGVPHAVVILPEISTLDLSILGPFLRYHQTFSPDGVNV
HHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCEEHHHHHHHHHHHHCCCCCCCCCE
NFVQILGHCQLRVRTYERGVEGETAACGTGALASALVVSNSYGWKESIQIHTWGGELMTV
EEEEEEECEEEEEEEECCCCCCCCCCCCCHHHHHEEEEECCCCCCEEEEEEEECCEEEEE
SQNRGRVYLQGSVTRDL
ECCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362