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The map label for this gene is gcsH

Identifier: 15835966

GI number: 15835966

Start: 476636

End: 476983

Strand: Reverse

Name: gcsH

Synonym: CPj0433

Alternate gene names: 15835966

Gene position: 476983-476636 (Counterclockwise)

Preceding gene: 15835967

Following gene: 15835965

Centisome position: 38.89

GC content: 41.95

Gene sequence:

>348_bases
ATGTGGTATTCTGATTATCATGTTTGGATTTTGCCCGTCCATGAGAGGGTGGTGCGCCTCGGGTTAACAGAAAAAATGCA
GAAAAATTTAGGAGCCATTCTCCATGTGGATTTACCTTCAGTAGGGAGTCTATGTAAAGAAGGTGAGGTTTTAGTCATTC
TGGAATCTTCTAAATCTGCTATAGAGGTGTTAAGTCCTGTATCAGGAGAGGTTATCGATATCAACCTTGATTTAGTGGAT
AATCCTCAGAAGATTAACGAAGCTCCAGAAGGTGAGGGATGGTTGGCTGTAGTCCGACTAGACCAGGACTGGGATCCTTC
TAATCTTTCTTTGATGGATGAAGAGTAA

Upstream 100 bases:

>100_bases
CTGCTAGCGTGGATAAACTATTTTTAGCTAAAAGTCAGCTAGATGAAGAACTTTTAGGATCCTATATGGAACGCTTTATA
GAACAGGGAGTCGTAAGGTG

Downstream 100 bases:

>100_bases
ATTTTTTATTAGATATACTCATTTTTTTCAGAAGATAAGAGGTATTTTTTTAAGGCTAAAACATTTAAAATTTATGTCTA
AGGTTTAAAAAATACATCAG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 115; Mature: 115

Protein sequence:

>115_residues
MWYSDYHVWILPVHERVVRLGLTEKMQKNLGAILHVDLPSVGSLCKEGEVLVILESSKSAIEVLSPVSGEVIDINLDLVD
NPQKINEAPEGEGWLAVVRLDQDWDPSNLSLMDEE

Sequences:

>Translated_115_residues
MWYSDYHVWILPVHERVVRLGLTEKMQKNLGAILHVDLPSVGSLCKEGEVLVILESSKSAIEVLSPVSGEVIDINLDLVD
NPQKINEAPEGEGWLAVVRLDQDWDPSNLSLMDEE
>Mature_115_residues
MWYSDYHVWILPVHERVVRLGLTEKMQKNLGAILHVDLPSVGSLCKEGEVLVILESSKSAIEVLSPVSGEVIDINLDLVD
NPQKINEAPEGEGWLAVVRLDQDWDPSNLSLMDEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=114, Percent_Identity=34.2105263157895, Blast_Score=63, Evalue=5e-11,
Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=34.2105263157895, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1789271, Length=97, Percent_Identity=35.0515463917526, Blast_Score=73, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17551294, Length=93, Percent_Identity=34.4086021505376, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319272, Length=100, Percent_Identity=38, Blast_Score=77, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=35.0877192982456, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CHLPN (Q9Z8B0)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C72079
- PIR:   G86544
- RefSeq:   NP_224633.1
- RefSeq:   NP_300490.1
- RefSeq:   NP_444869.1
- RefSeq:   NP_876723.1
- ProteinModelPortal:   Q9Z8B0
- SMR:   Q9Z8B0
- GeneID:   1467130
- GeneID:   894849
- GeneID:   919169
- GeneID:   963802
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0320
- KEGG:   cpn:CPn0433
- KEGG:   cpt:CpB0449
- TIGR:   CP_0320
- HOGENOM:   HBG693789
- OMA:   TPKELRY
- ProtClustDB:   PRK00624
- BioCyc:   CPNE115711:CP_0320-MONOMER
- BioCyc:   CPNE115713:CPN0433-MONOMER
- BioCyc:   CPNE138677:CPJ0433-MONOMER
- BioCyc:   CPNE182082:CPB0449-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017514
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR03077

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 12876; Mature: 12876

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYSDYHVWILPVHERVVRLGLTEKMQKNLGAILHVDLPSVGSLCKEGEVLVILESSKSA
CCCCCEEEEEEEHHHHHHHCCCHHHHHHCCCEEEEECCCCCHHHCCCCCEEEEECCCCCE
IEVLSPVSGEVIDINLDLVDNPQKINEAPEGEGWLAVVRLDQDWDPSNLSLMDEE
EEEECCCCCCEEEEEEEEECCCHHCCCCCCCCCEEEEEEECCCCCCCCCEEEECC
>Mature Secondary Structure
MWYSDYHVWILPVHERVVRLGLTEKMQKNLGAILHVDLPSVGSLCKEGEVLVILESSKSA
CCCCCEEEEEEEHHHHHHHCCCHHHHHHCCCEEEEECCCCCHHHCCCCCEEEEECCCCCE
IEVLSPVSGEVIDINLDLVDNPQKINEAPEGEGWLAVVRLDQDWDPSNLSLMDEE
EEEECCCCCCEEEEEEEEECCCHHCCCCCCCCCEEEEEEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362