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The map label for this gene is fabI

Identifier: 15835939

GI number: 15835939

Start: 451678

End: 452577

Strand: Direct

Name: fabI

Synonym: CPj0406

Alternate gene names: 15835939

Gene position: 451678-452577 (Clockwise)

Preceding gene: 15835936

Following gene: 15835941

Centisome position: 36.82

GC content: 43.78

Gene sequence:

>900_bases
ATGCTAAAGATTGATCTAACAGGAAAGGTAGCATTTGTTGCGGGCATTGGTGATGACCAAGGATATGGCTGGGGTATTGC
TAAACTTCTTGCAGAAGCAGGAGCTACGATTATTGTAGGAACATGGGTACCGATTTACAAAATTTTCTCTCAGTCTTGGG
AATTAGGAAAATTCAATGAATCTAGAAAATTATCGAATGGCACTCTCTTAGAGATTGCTAAGATCTATCCCATGGACGCA
AGTTTTGATAGCCCTGAAGATGTTCCTGAAGATATTGCTGAAAATAAACGTTACAAGGGCATTACGGGATTCACGATATC
AGAAGTCGCAGAACAGGTAAAAAAAGATTTTGGTCATATTGACATTCTTGTCCACTCGCTGGCAAATAGTCCTGAAATTT
CTAAGTCTCTATTAGAAACATCAAGAAAAGGTTACTTAGCGGCTCTCAGTGCCTCTAGTTATTCTTTTGTTAGCCTTCTC
TCTCACTTTGGAAGTATCATGAACCGTGGTGGATCGACAATATCGCTCACCTATTTGGCTTCTATGCGCGCTGTTCCTGG
ATACGGAGGGGGCATGAGTTCGGCAAAAGCAGCTTTGGAAAGTGACACCAAAACTCTTGCTTGGGAAGCGGGACGCCGTT
GGGGCATACGTGTCAATACCATCTCTGCAGGACCTTTAGCAAGCCGAGCTGGAAAAGCAATTGGTTTTATTGAAAGAATG
GTAGACTATTACCAAGAGTGGGCGCCTATTCCCGAGGCTATGAATGCCGAGCAGGTGGGTGCCGTTGCAGCTTTCTTAGC
ATCACCTCTAGCTTCAGCAATTACTGGTGAGACCTTATACGTAGATCACGGAGCCAATGTGATGGGAATTGGTCCTGAGA
TGTTCCCTAAAGACTCATAA

Upstream 100 bases:

>100_bases
GAGGCAGGATCTCCCCTGTTGAGGCACCAAAAGGTTTTCTTAATAATCGAAAGATTTGTTACTCTCCTTCCAATAAGCTT
CAAAAGTATAAGGCGGATTC

Downstream 100 bases:

>100_bases
GGTCGTCATAATAGCGGACACCAGCTTCCCAAGCTGAAAGAATGCCATTCTTATCTGCTGGGGGAGCTAGAAAGTCCGCA
TGAACGTGCATCTCTTCAGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLKIDLTGKVAFVAGIGDDQGYGWGIAKLLAEAGATIIVGTWVPIYKIFSQSWELGKFNESRKLSNGTLLEIAKIYPMDA
SFDSPEDVPEDIAENKRYKGITGFTISEVAEQVKKDFGHIDILVHSLANSPEISKSLLETSRKGYLAALSASSYSFVSLL
SHFGSIMNRGGSTISLTYLASMRAVPGYGGGMSSAKAALESDTKTLAWEAGRRWGIRVNTISAGPLASRAGKAIGFIERM
VDYYQEWAPIPEAMNAEQVGAVAAFLASPLASAITGETLYVDHGANVMGIGPEMFPKDS

Sequences:

>Translated_299_residues
MLKIDLTGKVAFVAGIGDDQGYGWGIAKLLAEAGATIIVGTWVPIYKIFSQSWELGKFNESRKLSNGTLLEIAKIYPMDA
SFDSPEDVPEDIAENKRYKGITGFTISEVAEQVKKDFGHIDILVHSLANSPEISKSLLETSRKGYLAALSASSYSFVSLL
SHFGSIMNRGGSTISLTYLASMRAVPGYGGGMSSAKAALESDTKTLAWEAGRRWGIRVNTISAGPLASRAGKAIGFIERM
VDYYQEWAPIPEAMNAEQVGAVAAFLASPLASAITGETLYVDHGANVMGIGPEMFPKDS
>Mature_299_residues
MLKIDLTGKVAFVAGIGDDQGYGWGIAKLLAEAGATIIVGTWVPIYKIFSQSWELGKFNESRKLSNGTLLEIAKIYPMDA
SFDSPEDVPEDIAENKRYKGITGFTISEVAEQVKKDFGHIDILVHSLANSPEISKSLLETSRKGYLAALSASSYSFVSLL
SHFGSIMNRGGSTISLTYLASMRAVPGYGGGMSSAKAALESDTKTLAWEAGRRWGIRVNTISAGPLASRAGKAIGFIERM
VDYYQEWAPIPEAMNAEQVGAVAAFLASPLASAITGETLYVDHGANVMGIGPEMFPKDS

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=285, Percent_Identity=29.8245614035088, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 31990; Mature: 31990

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIDLTGKVAFVAGIGDDQGYGWGIAKLLAEAGATIIVGTWVPIYKIFSQSWELGKFNE
CEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCCC
SRKLSNGTLLEIAKIYPMDASFDSPEDVPEDIAENKRYKGITGFTISEVAEQVKKDFGHI
CCCCCCCCEEEEHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHH
DILVHSLANSPEISKSLLETSRKGYLAALSASSYSFVSLLSHFGSIMNRGGSTISLTYLA
HHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
SMRAVPGYGGGMSSAKAALESDTKTLAWEAGRRWGIRVNTISAGPLASRAGKAIGFIERM
HHHCCCCCCCCHHHHHHHHHCCCHHEEHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
VDYYQEWAPIPEAMNAEQVGAVAAFLASPLASAITGETLYVDHGANVMGIGPEMFPKDS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure
MLKIDLTGKVAFVAGIGDDQGYGWGIAKLLAEAGATIIVGTWVPIYKIFSQSWELGKFNE
CEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCCC
SRKLSNGTLLEIAKIYPMDASFDSPEDVPEDIAENKRYKGITGFTISEVAEQVKKDFGHI
CCCCCCCCEEEEHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHH
DILVHSLANSPEISKSLLETSRKGYLAALSASSYSFVSLLSHFGSIMNRGGSTISLTYLA
HHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
SMRAVPGYGGGMSSAKAALESDTKTLAWEAGRRWGIRVNTISAGPLASRAGKAIGFIERM
HHHCCCCCCCCHHHHHHHHHCCCHHEEHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
VDYYQEWAPIPEAMNAEQVGAVAAFLASPLASAITGETLYVDHGANVMGIGPEMFPKDS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]