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The map label for this gene is folD

Identifier: 15835870

GI number: 15835870

Start: 379439

End: 380299

Strand: Direct

Name: folD

Synonym: CPj0335

Alternate gene names: 15835870

Gene position: 379439-380299 (Clockwise)

Preceding gene: 15835869

Following gene: 15835871

Centisome position: 30.94

GC content: 46.46

Gene sequence:

>861_bases
ATGTTACTGAGAGGGATTCCTGCAGCTGAAAAAATCCTTCAGAGACTCAAAGAGGAAATCTCACAAAGTCCTACCTCTCC
GGGGCTTGCTGTGGTCCTGATTGGCAATGACCCCGCATCTGAGGTGTACGTTGGCATGAAAGTCAAAAAAGCTACAGAAA
TCGGAATTATCTCCAAAGCGCACAAGTTACCCTCTGACTCTACCCTCTCCTCAGTCCTTAAGCTCATAGAACGATTGAAT
CAAGATCCTAGCATCCACGGCATCCTCGTGCAACTTCCCTTGCCCAAACACTTGGACAGCGAAGTGATTCTCCAAGCGAT
CTCCCCAGACAAAGATGTGGACGGGCTTCACCCTGTGAACATGGGAAAGTTGCTCCTTGGAAATTTTGATGGACTTCTAC
CCTGCACTCCTGCAGGAATTATTGAACTCCTGAACTATTATGAAATTCCTCTTCGAGGCCGCCATGCCGCTATTGTAGGG
AGAAGCAACATCGTGGGGAAACCCTTAGCGGCCCTCATGATGCAAAAGCATCCTCAAACTAACTGTACAGTCACAGTTCT
TCATAGCCAGTCGGAAAACCTCCCAGAAATCTTAAAGACAGCTGATATCATTATTGCTGCTCTAGGAGCACCGCTTTTTA
TAAAGGAAACTATGGTAGCCCCACATGCTGTGATCGTAGATGTAGGAACAACAAGAGTCCCTGCAGACAATGCGAAAGGC
TATACTCTTCTTGGAGATGTAGATTTTAATAACGTTGTGACAAAATGCGCAGCAATCACTCCAGTTCCTGGAGGCGTTGG
TCCCATGACTGTCGCTATGCTCATGAGCAATACATGGCGATGTTACCAAAATTTTTCTTAG

Upstream 100 bases:

>100_bases
CTTTCTTAGATAATCTCATCTTCACTTCTGTAATCTATATACTTCCTTGCGCAATAAACTCAGGAATCCATAAAATGATA
TCTTTTTTTAGGAGATTGGT

Downstream 100 bases:

>100_bases
TTCTTTTATGTCTTGGACTCTGTTCATGCTCTCAAAAAACGACAACAATCGAAGGAGAGCAGATGACAATCTTCTATCGC
ATTGTTCTGGGAACCTCTTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLLRGIPAAEKILQRLKEEISQSPTSPGLAVVLIGNDPASEVYVGMKVKKATEIGIISKAHKLPSDSTLSSVLKLIERLN
QDPSIHGILVQLPLPKHLDSEVILQAISPDKDVDGLHPVNMGKLLLGNFDGLLPCTPAGIIELLNYYEIPLRGRHAAIVG
RSNIVGKPLAALMMQKHPQTNCTVTVLHSQSENLPEILKTADIIIAALGAPLFIKETMVAPHAVIVDVGTTRVPADNAKG
YTLLGDVDFNNVVTKCAAITPVPGGVGPMTVAMLMSNTWRCYQNFS

Sequences:

>Translated_286_residues
MLLRGIPAAEKILQRLKEEISQSPTSPGLAVVLIGNDPASEVYVGMKVKKATEIGIISKAHKLPSDSTLSSVLKLIERLN
QDPSIHGILVQLPLPKHLDSEVILQAISPDKDVDGLHPVNMGKLLLGNFDGLLPCTPAGIIELLNYYEIPLRGRHAAIVG
RSNIVGKPLAALMMQKHPQTNCTVTVLHSQSENLPEILKTADIIIAALGAPLFIKETMVAPHAVIVDVGTTRVPADNAKG
YTLLGDVDFNNVVTKCAAITPVPGGVGPMTVAMLMSNTWRCYQNFS
>Mature_286_residues
MLLRGIPAAEKILQRLKEEISQSPTSPGLAVVLIGNDPASEVYVGMKVKKATEIGIISKAHKLPSDSTLSSVLKLIERLN
QDPSIHGILVQLPLPKHLDSEVILQAISPDKDVDGLHPVNMGKLLLGNFDGLLPCTPAGIIELLNYYEIPLRGRHAAIVG
RSNIVGKPLAALMMQKHPQTNCTVTVLHSQSENLPEILKTADIIIAALGAPLFIKETMVAPHAVIVDVGTTRVPADNAKG
YTLLGDVDFNNVVTKCAAITPVPGGVGPMTVAMLMSNTWRCYQNFS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=41.296928327645, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI222418558, Length=277, Percent_Identity=41.5162454873646, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI94721354, Length=288, Percent_Identity=40.9722222222222, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786741, Length=270, Percent_Identity=42.962962962963, Blast_Score=221, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=38.0622837370242, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319558, Length=283, Percent_Identity=43.4628975265018, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321643, Length=264, Percent_Identity=44.6969696969697, Blast_Score=218, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6322933, Length=298, Percent_Identity=27.5167785234899, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=43.1506849315069, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=43.1506849315069, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=43.1506849315069, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=43.1506849315069, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=39.7260273972603, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=39.5904436860068, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CHLPN (Q9Z8K3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   E72090
- PIR:   G86532
- RefSeq:   NP_224540.1
- RefSeq:   NP_300394.1
- RefSeq:   NP_444971.1
- RefSeq:   NP_876620.1
- ProteinModelPortal:   Q9Z8K3
- SMR:   Q9Z8K3
- PHCI-2DPAGE:   Q9Z8K3
- GeneID:   1467027
- GeneID:   894869
- GeneID:   919109
- GeneID:   962815
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0423
- KEGG:   cpn:CPn0335
- KEGG:   cpt:CpB0344
- TIGR:   CP_0423
- HOGENOM:   HBG328751
- OMA:   IGKLCLD
- PhylomeDB:   Q9Z8K3
- ProtClustDB:   PRK14181
- BioCyc:   CPNE115711:CP_0423-MONOMER
- BioCyc:   CPNE115713:CPN0335-MONOMER
- BioCyc:   CPNE138677:CPJ0335-MONOMER
- BioCyc:   CPNE182082:CPB0344-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30632; Mature: 30632

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRGIPAAEKILQRLKEEISQSPTSPGLAVVLIGNDPASEVYVGMKVKKATEIGIISKA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEEEEECHHHHHHHHHH
HKLPSDSTLSSVLKLIERLNQDPSIHGILVQLPLPKHLDSEVILQAISPDKDVDGLHPVN
HCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCC
MGKLLLGNFDGLLPCTPAGIIELLNYYEIPLRGRHAAIVGRSNIVGKPLAALMMQKHPQT
CCHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCC
NCTVTVLHSQSENLPEILKTADIIIAALGAPLFIKETMVAPHAVIVDVGTTRVPADNAKG
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCEEEEEECCCCCCCCCCCCC
YTLLGDVDFNNVVTKCAAITPVPGGVGPMTVAMLMSNTWRCYQNFS
EEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MLLRGIPAAEKILQRLKEEISQSPTSPGLAVVLIGNDPASEVYVGMKVKKATEIGIISKA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEEEEECHHHHHHHHHH
HKLPSDSTLSSVLKLIERLNQDPSIHGILVQLPLPKHLDSEVILQAISPDKDVDGLHPVN
HCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCC
MGKLLLGNFDGLLPCTPAGIIELLNYYEIPLRGRHAAIVGRSNIVGKPLAALMMQKHPQT
CCHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCC
NCTVTVLHSQSENLPEILKTADIIIAALGAPLFIKETMVAPHAVIVDVGTTRVPADNAKG
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCEEEEEECCCCCCCCCCCCC
YTLLGDVDFNNVVTKCAAITPVPGGVGPMTVAMLMSNTWRCYQNFS
EEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362