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The map label for this gene is nusA

Identifier: 15835851

GI number: 15835851

Start: 358442

End: 359746

Strand: Direct

Name: nusA

Synonym: CPj0316

Alternate gene names: 15835851

Gene position: 358442-359746 (Clockwise)

Preceding gene: 15835850

Following gene: 15835852

Centisome position: 29.22

GC content: 37.16

Gene sequence:

>1305_bases
ATGAATAAAAATCTTGTAGCTATTTTTGACTACATGGAGAAAGAAAAAGGGATTCAGCGCTCTACTATTATAGGAGCTAT
CGAATCTGCTTTAAAAATTGCTGCTAAAAAAACCTTAAGAGATGACGCGAACATATCTGTAAACATTAATTCTCGTACTG
GTGACATCGAAGTCTTTTGTGAAAAGGAAATAGTAGAAATTTGTCAGAATCCTAGCAAAGAAATTCCTTTAGATAAAGCC
AGAGAATACGATCCGGACTGTCAGATTGGTCAGTACATGGATGTCCCTTTTGTTTCTGATAATTTTGGAAGAATAGCTGC
TCACGCAGCACGACAAATTATCGGTCAAAAGCTAAGACATGCTGAGAGAGACGTTATTTATGAAGAATATCGCCATCGCG
TAAATGAAACTTTATCTGGTGTTGTCAAACGTTTTGCTAAAGGTTCTAATTTAATTATTGACTTAGGAAAAGTTGAAGCA
ATTCTTCCTACCCGGTTTTATCCTAAAACAGAAAAACATAAGATCGGTGATAAAATTTACGCCCTACTCTATGAAGTTCA
AGAGTCTGAAAATGGTGGAGCGGAAGTTATCCTCAGTCGTAGTCACGCAGAATTTGTTAAACAATTATTTATTCAAGAAG
TCCCAGAACTAGAAGAAGGTTCTGTGGAGATTGTTAAGATAGCTCGTGAAGCTGGGTACCGCACGAAACTAGCTGTAAGA
TCGTCAGACCCTAAAACTGATCCTGTTGGAGCTTTTGTAGGTATGCGAGGTTCTCGAGTAAAAAATATCATTCGAGAATT
GAACGATGAGAAAATTGACATTGTCAATTACTCCCCCGTCTCTACAGAGTTATTACAGAATCTTCTTTATCCAATAGAAA
TCCAAAAGATTGCTATTTTAGAAGACGACAAAGTGATTGCAATTGTCGTTAATGATGCAGACTACGCTACTGTTATTGGT
AAACGAGGAATTAACGCTCGTTTAATTAGCCACATTCTAGACTACGAGCTCGAAGTACAACGTATGAGTGAGTACAATAA
GTTGCTAGAAATTCAACGCCTTCAATTAGCAGAATTCGATAGTCCGCACTTAGATCAACCCTTAGAAATGGAAGGGATTA
GTAAGCTAGTCATCCAAAATTTAGAACATGCTGGATATGACACAATTAGAAGAGTATTATTAGCGAGTGCTAATGATCTG
GCATCTGTTCCTGGGATCAGTTTAGAGCTTGCTTATAAGATCCTTGAGCAAGTCAGCAAATATGGAGAAAGTAAAGTTGA
CGAAAAACCTGAAATTGAAGATTAA

Upstream 100 bases:

>100_bases
CATCTAATGCTGGTAATGCAGAGGATCGTATTATTTAGTTCTAAATAATACGTCCTTAATTTAGCTATTTACTGATTTCC
TTATTTACAAGAGGAGTATA

Downstream 100 bases:

>100_bases
GAATGCTCAATTAACGAAAGCCGCGGGGCTGGATAAGCTAAAACAAAAACTTGCCCAAGCAGGATCTTCTGAAGCTAAAT
CTTCTTCAGAAAAACCTTCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNKNLVAIFDYMEKEKGIQRSTIIGAIESALKIAAKKTLRDDANISVNINSRTGDIEVFCEKEIVEICQNPSKEIPLDKA
REYDPDCQIGQYMDVPFVSDNFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRVNETLSGVVKRFAKGSNLIIDLGKVEA
ILPTRFYPKTEKHKIGDKIYALLYEVQESENGGAEVILSRSHAEFVKQLFIQEVPELEEGSVEIVKIAREAGYRTKLAVR
SSDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVSTELLQNLLYPIEIQKIAILEDDKVIAIVVNDADYATVIG
KRGINARLISHILDYELEVQRMSEYNKLLEIQRLQLAEFDSPHLDQPLEMEGISKLVIQNLEHAGYDTIRRVLLASANDL
ASVPGISLELAYKILEQVSKYGESKVDEKPEIED

Sequences:

>Translated_434_residues
MNKNLVAIFDYMEKEKGIQRSTIIGAIESALKIAAKKTLRDDANISVNINSRTGDIEVFCEKEIVEICQNPSKEIPLDKA
REYDPDCQIGQYMDVPFVSDNFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRVNETLSGVVKRFAKGSNLIIDLGKVEA
ILPTRFYPKTEKHKIGDKIYALLYEVQESENGGAEVILSRSHAEFVKQLFIQEVPELEEGSVEIVKIAREAGYRTKLAVR
SSDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVSTELLQNLLYPIEIQKIAILEDDKVIAIVVNDADYATVIG
KRGINARLISHILDYELEVQRMSEYNKLLEIQRLQLAEFDSPHLDQPLEMEGISKLVIQNLEHAGYDTIRRVLLASANDL
ASVPGISLELAYKILEQVSKYGESKVDEKPEIED
>Mature_434_residues
MNKNLVAIFDYMEKEKGIQRSTIIGAIESALKIAAKKTLRDDANISVNINSRTGDIEVFCEKEIVEICQNPSKEIPLDKA
REYDPDCQIGQYMDVPFVSDNFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRVNETLSGVVKRFAKGSNLIIDLGKVEA
ILPTRFYPKTEKHKIGDKIYALLYEVQESENGGAEVILSRSHAEFVKQLFIQEVPELEEGSVEIVKIAREAGYRTKLAVR
SSDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVSTELLQNLLYPIEIQKIAILEDDKVIAIVVNDADYATVIG
KRGINARLISHILDYELEVQRMSEYNKLLEIQRLQLAEFDSPHLDQPLEMEGISKLVIQNLEHAGYDTIRRVLLASANDL
ASVPGISLELAYKILEQVSKYGESKVDEKPEIED

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=473, Percent_Identity=30.6553911205074, Blast_Score=218, Evalue=5e-58,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 49038; Mature: 49038

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNLVAIFDYMEKEKGIQRSTIIGAIESALKIAAKKTLRDDANISVNINSRTGDIEVFC
CCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
EKEIVEICQNPSKEIPLDKAREYDPDCQIGQYMDVPFVSDNFGRIAAHAARQIIGQKLRH
CHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AERDVIYEEYRHRVNETLSGVVKRFAKGSNLIIDLGKVEAILPTRFYPKTEKHKIGDKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEECCCCCCCCCHHHHHHHHHH
ALLYEVQESENGGAEVILSRSHAEFVKQLFIQEVPELEEGSVEIVKIAREAGYRTKLAVR
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEE
SSDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVSTELLQNLLYPIEIQKIAIL
CCCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEEE
EDDKVIAIVVNDADYATVIGKRGINARLISHILDYELEVQRMSEYNKLLEIQRLQLAEFD
ECCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
SPHLDQPLEMEGISKLVIQNLEHAGYDTIRRVLLASANDLASVPGISLELAYKILEQVSK
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHH
YGESKVDEKPEIED
HCCCCCCCCCCCCC
>Mature Secondary Structure
MNKNLVAIFDYMEKEKGIQRSTIIGAIESALKIAAKKTLRDDANISVNINSRTGDIEVFC
CCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
EKEIVEICQNPSKEIPLDKAREYDPDCQIGQYMDVPFVSDNFGRIAAHAARQIIGQKLRH
CHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AERDVIYEEYRHRVNETLSGVVKRFAKGSNLIIDLGKVEAILPTRFYPKTEKHKIGDKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEECCCCCCCCCHHHHHHHHHH
ALLYEVQESENGGAEVILSRSHAEFVKQLFIQEVPELEEGSVEIVKIAREAGYRTKLAVR
HHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEE
SSDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVSTELLQNLLYPIEIQKIAIL
CCCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEEE
EDDKVIAIVVNDADYATVIGKRGINARLISHILDYELEVQRMSEYNKLLEIQRLQLAEFD
ECCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
SPHLDQPLEMEGISKLVIQNLEHAGYDTIRRVLLASANDLASVPGISLELAYKILEQVSK
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHH
YGESKVDEKPEIED
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]