Click here to switch to the map view.

The map label for this gene is oppA_2

Identifier: 15835731

GI number: 15835731

Start: 235554

End: 237137

Strand: Direct

Name: oppA_2

Synonym: CPj0196

Alternate gene names: 15835731

Gene position: 235554-237137 (Clockwise)

Preceding gene: 15835730

Following gene: 15835732

Centisome position: 19.2

GC content: 40.03

Gene sequence:

>1584_bases
ATGCTCCGTTTCTTCGCTGTATTTATATCAACTCTTTGGCTCATTACCTCAGGATGTTCCCCATCCCAATCCTCTAAAGG
AATTTTTGTGGTAAATATGAAGGAAATGCCACGCTCCTTGGATCCTGGAAAAACTCGTCTCATTGCAGACCAAACTCTAA
TGCGTCATCTATATGAAGGACTCGTCGAAGAACATTCCCAAAATGGAGAGATTAAACCAGCCCTTGCAGAAAGCTACACC
ATCTCCGAAGACGGGACTCGGTACACATTTAAAATCAAAAACATCCTTTGGAGTAACGGAGACCCTCTGACAGCTCAAGA
CTTTGTCTCCTCTTGGAAGGAAATCCTAAAGGAAGATGCGTCCTCCGTATATCTCTATGCGTTTTTACCTATCAAAAATG
CTCGGGCAATCTTTGATGATACTGAGTCTCCAGAAAATCTAGGAGTCCGAGCTTTAGATAAGCGTCATCTCGAAATTCAG
TTAGAAACTCCCTGCGCGCATTTCCTACATTTCTTGACTCTTCCTATTTTTTTCCCTGTTCATGAAACTCTGCGAAACTA
TAGCACCTCTTTTGAAGAGATGCCCATTACCTGCGGTGCTTTCCGCCCTGTGTCTCTAGAAAAAGGCCTGAGACTCCATC
TAGAGAAAAACCCTATGTACCATAATAAAAGCCGTGTGAAACTACATAAAATTATTGTACAGTTTATCTCAAACGCTAAC
ACTGCAGCCATTCTATTCAAACATAAGAAATTAGATTGGCAAGGACCTCCTTGGGGAGAACCTATCCCTCCAGAAATCTC
AGCTTCTCTACATCAAGATGACCAGCTCTTTTCTCTTCCGGGCGCTTCGACTACATGGTTACTCTTTAATATACAAAAAA
AACCTTGGAACAATGCTAAATTACGCAAGGCATTGAGCCTTGCAATAGACAAAGATATGTTAACCAAAGTGGTATACCAA
GGTCTTGCAGAACCTACAGATCATATCCTACATCCAAGACTTTATCCAGGGACCTATCCCGAACGGAAAAGACAAAACGA
AAGAATTCTTGAGGCTCAACAACTCTTTGAAGAAGCTCTAGACGAACTTCAAATGACACGCGAAGATCTAGAAAAGGAAA
CTTTGACTTTCTCAACCTTTTCTTTTTCTTACGGAAGGATTTGCCAAATGCTAAGAGAACAATGGAAGAAAGTCTTAAAA
TTTACTATCCCTATAGTAGGCCAAGAGTTTTTCACAATACAAAAAAACTTCCTAGAGGGGAACTATTCCCTAACCGTGAA
CCAATGGACCGCAGCATTTATTGATCCGATGTCTTATCTCATGATCTTTGCCAATCCTGGAGGAATTTCCCCCTATCACC
TCCAAGATTCACACTTTCAAACTCTTCTCATAAAGATCACTCAAGAACATAAAAAACACCTACGAAATCAGCTTATTATT
GAAGCCCTTGACTATTTAGAACACTGTCACATTCTCGAACCACTATGTCATCCAAATCTTCGAATTGCTTTGAACAAAAA
CATTAAAAACTTTAATCTTTTTGTTCGACGAACTTCAGACTTTCGTTTTATAGAAAAACTATAG

Upstream 100 bases:

>100_bases
TGGGTGCCTTTGTGGCACCTCGTTTCCTTCTGACTGCTCTTCTCTCTCTACTCAACCGCATCCCTAAAAGTTGAAATCTT
ATTCTAAAGAAAGGTCTTTT

Downstream 100 bases:

>100_bases
GAGAAAAAGTTTAGACTTAAAATTCGATAGTAAATTTATTAAAGTAATTTTTAAAATGTTTTCACGATGGATCACCCTCT
TTTTATTATTCATTAGCCTT

Product: oligopeptide binding protein

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MLRFFAVFISTLWLITSGCSPSQSSKGIFVVNMKEMPRSLDPGKTRLIADQTLMRHLYEGLVEEHSQNGEIKPALAESYT
ISEDGTRYTFKIKNILWSNGDPLTAQDFVSSWKEILKEDASSVYLYAFLPIKNARAIFDDTESPENLGVRALDKRHLEIQ
LETPCAHFLHFLTLPIFFPVHETLRNYSTSFEEMPITCGAFRPVSLEKGLRLHLEKNPMYHNKSRVKLHKIIVQFISNAN
TAAILFKHKKLDWQGPPWGEPIPPEISASLHQDDQLFSLPGASTTWLLFNIQKKPWNNAKLRKALSLAIDKDMLTKVVYQ
GLAEPTDHILHPRLYPGTYPERKRQNERILEAQQLFEEALDELQMTREDLEKETLTFSTFSFSYGRICQMLREQWKKVLK
FTIPIVGQEFFTIQKNFLEGNYSLTVNQWTAAFIDPMSYLMIFANPGGISPYHLQDSHFQTLLIKITQEHKKHLRNQLII
EALDYLEHCHILEPLCHPNLRIALNKNIKNFNLFVRRTSDFRFIEKL

Sequences:

>Translated_527_residues
MLRFFAVFISTLWLITSGCSPSQSSKGIFVVNMKEMPRSLDPGKTRLIADQTLMRHLYEGLVEEHSQNGEIKPALAESYT
ISEDGTRYTFKIKNILWSNGDPLTAQDFVSSWKEILKEDASSVYLYAFLPIKNARAIFDDTESPENLGVRALDKRHLEIQ
LETPCAHFLHFLTLPIFFPVHETLRNYSTSFEEMPITCGAFRPVSLEKGLRLHLEKNPMYHNKSRVKLHKIIVQFISNAN
TAAILFKHKKLDWQGPPWGEPIPPEISASLHQDDQLFSLPGASTTWLLFNIQKKPWNNAKLRKALSLAIDKDMLTKVVYQ
GLAEPTDHILHPRLYPGTYPERKRQNERILEAQQLFEEALDELQMTREDLEKETLTFSTFSFSYGRICQMLREQWKKVLK
FTIPIVGQEFFTIQKNFLEGNYSLTVNQWTAAFIDPMSYLMIFANPGGISPYHLQDSHFQTLLIKITQEHKKHLRNQLII
EALDYLEHCHILEPLCHPNLRIALNKNIKNFNLFVRRTSDFRFIEKL
>Mature_527_residues
MLRFFAVFISTLWLITSGCSPSQSSKGIFVVNMKEMPRSLDPGKTRLIADQTLMRHLYEGLVEEHSQNGEIKPALAESYT
ISEDGTRYTFKIKNILWSNGDPLTAQDFVSSWKEILKEDASSVYLYAFLPIKNARAIFDDTESPENLGVRALDKRHLEIQ
LETPCAHFLHFLTLPIFFPVHETLRNYSTSFEEMPITCGAFRPVSLEKGLRLHLEKNPMYHNKSRVKLHKIIVQFISNAN
TAAILFKHKKLDWQGPPWGEPIPPEISASLHQDDQLFSLPGASTTWLLFNIQKKPWNNAKLRKALSLAIDKDMLTKVVYQ
GLAEPTDHILHPRLYPGTYPERKRQNERILEAQQLFEEALDELQMTREDLEKETLTFSTFSFSYGRICQMLREQWKKVLK
FTIPIVGQEFFTIQKNFLEGNYSLTVNQWTAAFIDPMSYLMIFANPGGISPYHLQDSHFQTLLIKITQEHKKHLRNQLII
EALDYLEHCHILEPLCHPNLRIALNKNIKNFNLFVRRTSDFRFIEKL

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=499, Percent_Identity=27.2545090180361, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081878, Length=490, Percent_Identity=27.5510204081633, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787495, Length=488, Percent_Identity=24.5901639344262, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789966, Length=400, Percent_Identity=25.25, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787052, Length=311, Percent_Identity=23.7942122186495, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1787762, Length=538, Percent_Identity=22.3048327137546, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789887, Length=409, Percent_Identity=24.9388753056235, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787551, Length=327, Percent_Identity=24.1590214067278, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61167; Mature: 61167

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFFAVFISTLWLITSGCSPSQSSKGIFVVNMKEMPRSLDPGKTRLIADQTLMRHLYEG
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCCCCCCEEEHHHHHHHHHHHHH
LVEEHSQNGEIKPALAESYTISEDGTRYTFKIKNILWSNGDPLTAQDFVSSWKEILKEDA
HHHHCCCCCCCCCCHHCCEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
SSVYLYAFLPIKNARAIFDDTESPENLGVRALDKRHLEIQLETPCAHFLHFLTLPIFFPV
CCEEEEEEEECCCCCEECCCCCCCHHCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHH
HETLRNYSTSFEEMPITCGAFRPVSLEKGLRLHLEKNPMYHNKSRVKLHKIIVQFISNAN
HHHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
TAAILFKHKKLDWQGPPWGEPIPPEISASLHQDDQLFSLPGASTTWLLFNIQKKPWNNAK
EEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCEEECCCCCEEEEEEEEECCCCCCHH
LRKALSLAIDKDMLTKVVYQGLAEPTDHILHPRLYPGTYPERKRQNERILEAQQLFEEAL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DELQMTREDLEKETLTFSTFSFSYGRICQMLREQWKKVLKFTIPIVGQEFFTIQKNFLEG
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
NYSLTVNQWTAAFIDPMSYLMIFANPGGISPYHLQDSHFQTLLIKITQEHKKHLRNQLII
CEEEEEECHHHHHHCCCEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHH
EALDYLEHCHILEPLCHPNLRIALNKNIKNFNLFVRRTSDFRFIEKL
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCHHHHCC
>Mature Secondary Structure
MLRFFAVFISTLWLITSGCSPSQSSKGIFVVNMKEMPRSLDPGKTRLIADQTLMRHLYEG
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCCCCCCCEEEHHHHHHHHHHHHH
LVEEHSQNGEIKPALAESYTISEDGTRYTFKIKNILWSNGDPLTAQDFVSSWKEILKEDA
HHHHCCCCCCCCCCHHCCEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
SSVYLYAFLPIKNARAIFDDTESPENLGVRALDKRHLEIQLETPCAHFLHFLTLPIFFPV
CCEEEEEEEECCCCCEECCCCCCCHHCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHH
HETLRNYSTSFEEMPITCGAFRPVSLEKGLRLHLEKNPMYHNKSRVKLHKIIVQFISNAN
HHHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
TAAILFKHKKLDWQGPPWGEPIPPEISASLHQDDQLFSLPGASTTWLLFNIQKKPWNNAK
EEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCEEECCCCCEEEEEEEEECCCCCCHH
LRKALSLAIDKDMLTKVVYQGLAEPTDHILHPRLYPGTYPERKRQNERILEAQQLFEEAL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DELQMTREDLEKETLTFSTFSFSYGRICQMLREQWKKVLKFTIPIVGQEFFTIQKNFLEG
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
NYSLTVNQWTAAFIDPMSYLMIFANPGGISPYHLQDSHFQTLLIKITQEHKKHLRNQLII
CEEEEEECHHHHHHCCCEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHH
EALDYLEHCHILEPLCHPNLRIALNKNIKNFNLFVRRTSDFRFIEKL
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]