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The map label for this gene is guaA

Identifier: 15835706

GI number: 15835706

Start: 210769

End: 212058

Strand: Reverse

Name: guaA

Synonym: CPj0171

Alternate gene names: 15835706

Gene position: 212058-210769 (Counterclockwise)

Preceding gene: 15835707

Following gene: 15835705

Centisome position: 17.29

GC content: 41.16

Gene sequence:

>1290_bases
TTGCAGAGTGCAAGGAGACATTTGAACACCATATTTATTCTAGATTTTGGATCTCAATATACTTATGTATTAGCAAAGCA
AGTGCGGAAGTTATTTGTATATTGCGAAGTTCTTCCCTGGAATATCTCTGTGCAATGTTTAAAAGAAAGAGCGCCTTTGG
GGATCATTCTCTCAGGAGGTCCTCACTCTGTCTATGAAAACAAGGCTCCACATTTAGATCCTGAAATCTATAAACTTGGC
ATTCCAATTCTAGCTATTTGCTATGGCATGCAGCTTATGGCTAGAGATTTTGGAGGGACTGTAAGCCCTGGTGTAGGAGA
ATTTGGATATACGCCCATCCATCTGTATCCTTGTGAGCTCTTCAAACACATCGTCGACTGCGAATCTCTAGACACAGAGA
TTCGGATGAGCCATCGGGATCATGTTACGACAATTCCTGAAGGATTTAATGTAATCGCATCCACCTCACAATGCTCGATC
TCAGGAATAGAAAATACCAAACAACGGTTGTACGGGCTGCAATTTCATCCCGAGGTTTCTGACTCCACTCCAACGGGAAA
TAAGATTCTAGAAACTTTTGTTCAAGAGATCTGTTCTGCTCCCACACTATGGAATCCCTTGTATATTCAGCAAGACCTTG
TAAGTAAAATTCAAGATACCGTTATTGAAGTATTTGATGAAGTCGCTCAGTCATTAGACGTACAATGGTTAGCTCAAGGA
ACCATCTACTCAGATGTTATTGAGTCCTCACGCTCTGGACATGCCTCCGAAGTAATAAAATCACATCATAATGTAGGGGG
GCTTCCAAAAAATCTTAAGCTGAAGTTAGTCGAGCCCTTACGTTATTTATTTAAAGATGAAGTTCGAATTTTAGGAGAAG
CCCTAGGACTTTCTAGCTATCTCTTGGACAGGCATCCTTTTCCTGGACCTGGCTTGACAATTCGTGTGATTGGAGAGATC
CTTCCTGAATATCTAGCCATTTTACGACGGGCGGACCTCATCTTTATAGAAGAGCTTAGGAAAGCAAAACTCTACGATAA
AATAAGCCAAGCCTTTGCTCTATTTCTTCCTATAAAATCAGTATCTGTAAAAGGAGATTGTAGAAGCTATGGTTATACCA
TAGCATTACGTGCTGTAGAATCTACAGATTTCATGACAGGACGATGGGCCTACCTTCCATGCGATGTTCTCAGTTCTTGC
TCATCGCGAATTATTAATGAAATACCCGAGGTAAGCCGAGTGGTCTATGATATTTCTGACAAGCCACCAGCAACTATAGA
ATGGGAATAG

Upstream 100 bases:

>100_bases
TTAAAAACTAAGGCTTCCTTTGTTCGAATTACTGAATCTGGAAGAGCTGAAAGTCATATTCATAATATTTACAAAGTTCA
ACCAACCTTAAATTATTAAG

Downstream 100 bases:

>100_bases
CCACCATAGGGGTAAATCAAAGACTCGTTATTTAGTAACATCATTAGAATGTTCGTAAAAATCATATCATTAGAGGAGAA
AAGTATAACACTTGCACAGT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQSARRHLNTIFILDFGSQYTYVLAKQVRKLFVYCEVLPWNISVQCLKERAPLGIILSGGPHSVYENKAPHLDPEIYKLG
IPILAICYGMQLMARDFGGTVSPGVGEFGYTPIHLYPCELFKHIVDCESLDTEIRMSHRDHVTTIPEGFNVIASTSQCSI
SGIENTKQRLYGLQFHPEVSDSTPTGNKILETFVQEICSAPTLWNPLYIQQDLVSKIQDTVIEVFDEVAQSLDVQWLAQG
TIYSDVIESSRSGHASEVIKSHHNVGGLPKNLKLKLVEPLRYLFKDEVRILGEALGLSSYLLDRHPFPGPGLTIRVIGEI
LPEYLAILRRADLIFIEELRKAKLYDKISQAFALFLPIKSVSVKGDCRSYGYTIALRAVESTDFMTGRWAYLPCDVLSSC
SSRIINEIPEVSRVVYDISDKPPATIEWE

Sequences:

>Translated_429_residues
MQSARRHLNTIFILDFGSQYTYVLAKQVRKLFVYCEVLPWNISVQCLKERAPLGIILSGGPHSVYENKAPHLDPEIYKLG
IPILAICYGMQLMARDFGGTVSPGVGEFGYTPIHLYPCELFKHIVDCESLDTEIRMSHRDHVTTIPEGFNVIASTSQCSI
SGIENTKQRLYGLQFHPEVSDSTPTGNKILETFVQEICSAPTLWNPLYIQQDLVSKIQDTVIEVFDEVAQSLDVQWLAQG
TIYSDVIESSRSGHASEVIKSHHNVGGLPKNLKLKLVEPLRYLFKDEVRILGEALGLSSYLLDRHPFPGPGLTIRVIGEI
LPEYLAILRRADLIFIEELRKAKLYDKISQAFALFLPIKSVSVKGDCRSYGYTIALRAVESTDFMTGRWAYLPCDVLSSC
SSRIINEIPEVSRVVYDISDKPPATIEWE
>Mature_429_residues
MQSARRHLNTIFILDFGSQYTYVLAKQVRKLFVYCEVLPWNISVQCLKERAPLGIILSGGPHSVYENKAPHLDPEIYKLG
IPILAICYGMQLMARDFGGTVSPGVGEFGYTPIHLYPCELFKHIVDCESLDTEIRMSHRDHVTTIPEGFNVIASTSQCSI
SGIENTKQRLYGLQFHPEVSDSTPTGNKILETFVQEICSAPTLWNPLYIQQDLVSKIQDTVIEVFDEVAQSLDVQWLAQG
TIYSDVIESSRSGHASEVIKSHHNVGGLPKNLKLKLVEPLRYLFKDEVRILGEALGLSSYLLDRHPFPGPGLTIRVIGEI
LPEYLAILRRADLIFIEELRKAKLYDKISQAFALFLPIKSVSVKGDCRSYGYTIALRAVESTDFMTGRWAYLPCDVLSSC
SSRIINEIPEVSRVVYDISDKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=280, Percent_Identity=33.9285714285714, Blast_Score=142, Evalue=8e-34,
Organism=Escherichia coli, GI1788854, Length=210, Percent_Identity=66.1904761904762, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI133901714, Length=219, Percent_Identity=39.2694063926941, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71992717, Length=219, Percent_Identity=39.2694063926941, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71992710, Length=219, Percent_Identity=39.2694063926941, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323873, Length=219, Percent_Identity=56.1643835616438, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI281365319, Length=284, Percent_Identity=33.4507042253521, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_CHLPN (Q9Z913)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72109
- PIR:   C86512
- RefSeq:   NP_224379.1
- RefSeq:   NP_300230.1
- RefSeq:   NP_445141.1
- RefSeq:   NP_876449.1
- ProteinModelPortal:   Q9Z913
- SMR:   Q9Z913
- GeneID:   1466856
- GeneID:   895050
- GeneID:   918965
- GeneID:   963335
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0599
- KEGG:   cpn:CPn0171
- KEGG:   cpt:CpB0173
- TIGR:   CP_0599
- HOGENOM:   HBG391775
- OMA:   PSTIEPC
- ProtClustDB:   CLSK2565923
- BioCyc:   CPNE115711:CP_0599-MONOMER
- BioCyc:   CPNE115713:CPN0171-MONOMER
- BioCyc:   CPNE138677:CPJ0171-MONOMER
- BioCyc:   CPNE182082:CPB0173-MONOMER
- BRENDA:   6.3.5.2
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C

EC number: =6.3.5.2

Molecular weight: Translated: 48243; Mature: 48243

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 87-87 ACT_SITE 176-176 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSARRHLNTIFILDFGSQYTYVLAKQVRKLFVYCEVLPWNISVQCLKERAPLGIILSGG
CCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCCEEEEECCC
PHSVYENKAPHLDPEIYKLGIPILAICYGMQLMARDFGGTVSPGVGEFGYTPIHLYPCEL
CCCHHCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHH
FKHIVDCESLDTEIRMSHRDHVTTIPEGFNVIASTSQCSISGIENTKQRLYGLQFHPEVS
HHHHHCHHHCCHHHHHCCCCCCCCCCCCCEEEECCCCCEECCCHHHHHHHCCCEECCCCC
DSTPTGNKILETFVQEICSAPTLWNPLYIQQDLVSKIQDTVIEVFDEVAQSLDVQWLAQG
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TIYSDVIESSRSGHASEVIKSHHNVGGLPKNLKLKLVEPLRYLFKDEVRILGEALGLSSY
CHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLDRHPFPGPGLTIRVIGEILPEYLAILRRADLIFIEELRKAKLYDKISQAFALFLPIKS
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVKGDCRSYGYTIALRAVESTDFMTGRWAYLPCDVLSSCSSRIINEIPEVSRVVYDISD
CEECCCHHHCCEEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
KPPATIEWE
CCCCCEECC
>Mature Secondary Structure
MQSARRHLNTIFILDFGSQYTYVLAKQVRKLFVYCEVLPWNISVQCLKERAPLGIILSGG
CCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCCEEEEECCC
PHSVYENKAPHLDPEIYKLGIPILAICYGMQLMARDFGGTVSPGVGEFGYTPIHLYPCEL
CCCHHCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHH
FKHIVDCESLDTEIRMSHRDHVTTIPEGFNVIASTSQCSISGIENTKQRLYGLQFHPEVS
HHHHHCHHHCCHHHHHCCCCCCCCCCCCCEEEECCCCCEECCCHHHHHHHCCCEECCCCC
DSTPTGNKILETFVQEICSAPTLWNPLYIQQDLVSKIQDTVIEVFDEVAQSLDVQWLAQG
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TIYSDVIESSRSGHASEVIKSHHNVGGLPKNLKLKLVEPLRYLFKDEVRILGEALGLSSY
CHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLDRHPFPGPGLTIRVIGEILPEYLAILRRADLIFIEELRKAKLYDKISQAFALFLPIKS
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVKGDCRSYGYTIALRAVESTDFMTGRWAYLPCDVLSSCSSRIINEIPEVSRVVYDISD
CEECCCHHHCCEEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
KPPATIEWE
CCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362