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The map label for this gene is pfkA_1

Identifier: 15835695

GI number: 15835695

Start: 201780

End: 203414

Strand: Reverse

Name: pfkA_1

Synonym: CPj0160

Alternate gene names: 15835695

Gene position: 203414-201780 (Counterclockwise)

Preceding gene: 15835696

Following gene: 15835694

Centisome position: 16.58

GC content: 39.51

Gene sequence:

>1635_bases
GTGGAACTTCTCTCGTTAAATAAAAGTTATTTTGAAATCCAACGCCTTCGCTACCGCCCTGAGATTCTTACTTTATTAGA
AACGATACGTTCGAAACATATCCAAGAAACTTCGTCTCCCCCTTCTCCCCCTCCAGAGTTGCAAAAACACATTCCCAATC
TATGTCGGATCCCTGAAGTTTCTATTTATACTGAACAGGAAACGTCATCAAAACCATTAAAGATTGGGGTTTTACTATCA
GGAGGACAAGCTCCTGGGGGGCATAATGTCGTTATTGGTCTTTTTGATGCTTTACGAGTATTCAATCCCAAGACTCGCTT
ATTTGGATTCATCAAAGGACCTTTAGGGCTTACTCGTGGGCTCTATAAAGATCTGGATATCTCCGTAATCTATGATTATT
ACAACATGGGAGGGTTCGACATGCTCTCCTCAAGCCGAGAAAAAATTAAAACTGAAGAGCAGAAAAAAAACATCCTCAAC
ACAGTCAAGCAACTAAAGTTAGACGGACTACTCATTATAGGAGGAAATAATTCCAATACGGACACTGCGATGCTTGCAGA
ATATTTCCTTGCTCACAACTGCAAAACATCCGTGATTGGAGTCCCTAAAACTATAGATGGTGATCTTAAGAACTGTTGGA
TTGAAACCTCGTTAGGTTTTCATACTTCTTGTCGCACGTACTCAGAAATGATTGGGAATCTTGCTAAAGATGCTCTTTCT
GCAAAAAAATACCACCATTTCATCCGCCTTATGGGGCAGCAGGCCTCTTATACTACTTTAGAATGCGGATTGCAGACCCT
CCCTAATATAGCTTTAATTAGCGAACTTATTGCCACTAGGAAAATCTCTTTAAAGCAACTGAGTGAACAGCTTGCTTTAG
GTTTGGTACGCCGCTATAAATCTGGAAAAAACTATAGTACTGTGTTAATCCCAGAGGGACTGATTGAGCATATTTTCGAT
ACACGCAAGCTTATAGATGAACTCAATGTTTTACTCGCCAATGGAGATTCTTCTATTGAAAAAATTCTTTCCAAGCTCTC
TCCAGAGACATTAAAAACATTTCACTTGTTTCCTAAGGATATTGCGAACCAACTTCTTCTAGCTCGAGATTCTCATGGAA
ATGTTAGAGTTTCAAAAATTGCTACAGAAGAGCTGCTTGCAGTAATGGTAAAGAAGGAAATCGAAAAGATAAAACCCCAT
ATGGAATTCCATTCTGTATCGCATTTTTTTGGTTATGAAGCACGAGCAGGATTCCCTTCCAACTTTGATTGTAATTATGG
AATCGCTTTAGGGATCATTTCTGCGTTATTTCTGGTCCGACAAAAGACTGGTTATATGATTACAATCAATAATCTTGCTC
AATCTTATACTGAATGGCAAGGGGGAGCCACTCCATTATATAAGATGATGCACTTAGAAAATCGTTGTGGGACAGAGACT
CCTGTTATTAAAACAGATTCTGTAGACCCAAAATCCCCTGCTGTTCAACATTTACTTCAACAAAGTGATTCTTGTTTGGT
TGAAGATTTATACCGTTTTCCAGGCCCTTTACAGTACTTCGGGAAAGAGGAGCTTATAGATCAACGACCTTTGACCCTGC
TTTGGGAAAATCAAACGCATTCGCCTCTACTCTGA

Upstream 100 bases:

>100_bases
TTATCCCGATGTCGATCACGCGTTTCCTTTTGCCGAATCTTCGGCTCTTTCGGATCTCACGCAATGGTTAAAAAGAGAAT
TAACTTCTGGGGAATAAACT

Downstream 100 bases:

>100_bases
CAACCTGTCCATTCCTATAAGAATATTCTAAGCTAGATGCAAAGGAACGGCTCTTTTGTGTTAAGATTTCTTAAGCTTTA
AAAAAACATCTATATTAAAA

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEVSIYTEQETSSKPLKIGVLLS
GGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRGLYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILN
TVKQLKLDGLLIIGGNNSNTDTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYKSGKNYSTVLIPEGLIEHIFD
TRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKDIANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPH
MEFHSVSHFFGYEARAGFPSNFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTHSPLL

Sequences:

>Translated_544_residues
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEVSIYTEQETSSKPLKIGVLLS
GGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRGLYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILN
TVKQLKLDGLLIIGGNNSNTDTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYKSGKNYSTVLIPEGLIEHIFD
TRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKDIANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPH
MEFHSVSHFFGYEARAGFPSNFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTHSPLL
>Mature_544_residues
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEVSIYTEQETSSKPLKIGVLLS
GGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRGLYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILN
TVKQLKLDGLLIIGGNNSNTDTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYKSGKNYSTVLIPEGLIEHIFD
TRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKDIANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPH
MEFHSVSHFFGYEARAGFPSNFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTHSPLL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=251, Percent_Identity=27.0916334661355, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 61203; Mature: 61203

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEV
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHCCCCC
SIYTEQETSSKPLKIGVLLSGGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRG
EEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHCCCHHHHHEEECCCHHHHHH
LYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILNTVKQLKLDGLLIIGGNNSNT
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
HHHHHHHHHHHCCCCCCEEECCCCCCCCHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHH
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYK
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKNYSTVLIPEGLIEHIFDTRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKD
CCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCHHHHHHHHCCHHH
IANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPHMEFHSVSHFFGYEARAGFPS
HHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
NFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTH
CEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCEEEECCCCC
SPLL
CCCC
>Mature Secondary Structure
MELLSLNKSYFEIQRLRYRPEILTLLETIRSKHIQETSSPPSPPPELQKHIPNLCRIPEV
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHCCCCC
SIYTEQETSSKPLKIGVLLSGGQAPGGHNVVIGLFDALRVFNPKTRLFGFIKGPLGLTRG
EEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHCCCHHHHHEEECCCHHHHHH
LYKDLDISVIYDYYNMGGFDMLSSSREKIKTEEQKKNILNTVKQLKLDGLLIIGGNNSNT
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DTAMLAEYFLAHNCKTSVIGVPKTIDGDLKNCWIETSLGFHTSCRTYSEMIGNLAKDALS
HHHHHHHHHHHCCCCCCEEECCCCCCCCHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHH
AKKYHHFIRLMGQQASYTTLECGLQTLPNIALISELIATRKISLKQLSEQLALGLVRRYK
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKNYSTVLIPEGLIEHIFDTRKLIDELNVLLANGDSSIEKILSKLSPETLKTFHLFPKD
CCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCHHHHHHHHCCHHH
IANQLLLARDSHGNVRVSKIATEELLAVMVKKEIEKIKPHMEFHSVSHFFGYEARAGFPS
HHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
NFDCNYGIALGIISALFLVRQKTGYMITINNLAQSYTEWQGGATPLYKMMHLENRCGTET
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
PVIKTDSVDPKSPAVQHLLQQSDSCLVEDLYRFPGPLQYFGKEELIDQRPLTLLWENQTH
CEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCEEEECCCCC
SPLL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA