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The map label for this gene is rnhB

Identifier: 15835655

GI number: 15835655

Start: 150234

End: 150878

Strand: Direct

Name: rnhB

Synonym: CPj0119

Alternate gene names: 15835655

Gene position: 150234-150878 (Clockwise)

Preceding gene: 15835654

Following gene: 15835656

Centisome position: 12.25

GC content: 40.31

Gene sequence:

>645_bases
ATGAATACTTCTATTTCTGAAATTCAGCGTTTTCTTTCTATGATTGCTTTTGAGAAAGAGCTCGTCTCAGAAGATTTTAG
TGTCGTCGCTGGAATAGATGAAGCTGGAAGAGGGCCACTGGCAGGTCCCGTAGTTGCTAGTGCCTGTATTTTACCTAAGG
GAAAGGTATTTCCTGGAGTAAATGATAGTAAGAAGCTATCTCCTAAACAACGAGCCCAAGTTCGGGATGCTTTGATGCAA
GATCCTGAGGTCTGTTTTGGTATAGGCGTAATTTCTGTAGAGAGGATAGATCAAGTTAACATTTTAGAAGCCACTAAAGA
GGCTATGCTTCAAGCAATATCTTCTTTACCGATATCTCCAGATATTCTTCTTGTGGATGGTCTTTATTTACCCCATGACA
TTCCTTGTAAGAAAATCATTCAAGGAGATGCTAAATCTGCATCCATAGCGGCGGCTTCTATTTTAGCAAAAGAACATCGT
GATGATTTGATGTTACAACTACACAGGCTCTATCCTGAATATGGATTTGATAGACATAAGGGATACGGAACTTCCTTGCA
TGTAGAAGCAATACGACGTTATGGTCCCAGTCCCTGCCATAGGAAGAGCTTTTCTCCAATAAAGCAAATGTGTGCTATTG
TATGA

Upstream 100 bases:

>100_bases
GCTAAAGTTAAAGAGTTTGTAGGACCTAGATCTTCAAAGAAATAGTCTGTAGCAAGACTTCATATTGTCTTATTTTGATT
TTTATAATCTATAGTAGCTT

Downstream 100 bases:

>100_bases
ATAAGATCCTAGTTGACTCTCCTTTTTCTCCAGATCACCAGAAGTGCTGTCCTAAGCTTTTTACAATTAGTGCTCCTGCT
GGAGTTGGAAAGACAACACT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNTSISEIQRFLSMIAFEKELVSEDFSVVAGIDEAGRGPLAGPVVASACILPKGKVFPGVNDSKKLSPKQRAQVRDALMQ
DPEVCFGIGVISVERIDQVNILEATKEAMLQAISSLPISPDILLVDGLYLPHDIPCKKIIQGDAKSASIAAASILAKEHR
DDLMLQLHRLYPEYGFDRHKGYGTSLHVEAIRRYGPSPCHRKSFSPIKQMCAIV

Sequences:

>Translated_214_residues
MNTSISEIQRFLSMIAFEKELVSEDFSVVAGIDEAGRGPLAGPVVASACILPKGKVFPGVNDSKKLSPKQRAQVRDALMQ
DPEVCFGIGVISVERIDQVNILEATKEAMLQAISSLPISPDILLVDGLYLPHDIPCKKIIQGDAKSASIAAASILAKEHR
DDLMLQLHRLYPEYGFDRHKGYGTSLHVEAIRRYGPSPCHRKSFSPIKQMCAIV
>Mature_214_residues
MNTSISEIQRFLSMIAFEKELVSEDFSVVAGIDEAGRGPLAGPVVASACILPKGKVFPGVNDSKKLSPKQRAQVRDALMQ
DPEVCFGIGVISVERIDQVNILEATKEAMLQAISSLPISPDILLVDGLYLPHDIPCKKIIQGDAKSASIAAASILAKEHR
DDLMLQLHRLYPEYGFDRHKGYGTSLHVEAIRRYGPSPCHRKSFSPIKQMCAIV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=195, Percent_Identity=40.5128205128205, Blast_Score=151, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CHLPN (Q9Z962)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   E72117
- PIR:   H86505
- RefSeq:   NP_224327.1
- RefSeq:   NP_300179.1
- RefSeq:   NP_445196.1
- RefSeq:   NP_876396.1
- ProteinModelPortal:   Q9Z962
- SMR:   Q9Z962
- GeneID:   1466803
- GeneID:   894649
- GeneID:   918907
- GeneID:   963710
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0654
- KEGG:   cpn:CPn0119
- KEGG:   cpt:CpB0120
- TIGR:   CP_0654
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   CPNE115711:CP_0654-MONOMER
- BioCyc:   CPNE115713:CPN0119-MONOMER
- BioCyc:   CPNE138677:CPJ0119-MONOMER
- BioCyc:   CPNE182082:CPB0120-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23411; Mature: 23411

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSISEIQRFLSMIAFEKELVSEDFSVVAGIDEAGRGPLAGPVVASACILPKGKVFPGV
CCCCHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
NDSKKLSPKQRAQVRDALMQDPEVCFGIGVISVERIDQVNILEATKEAMLQAISSLPISP
CCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
DILLVDGLYLPHDIPCKKIIQGDAKSASIAAASILAKEHRDDLMLQLHRLYPEYGFDRHK
CEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GYGTSLHVEAIRRYGPSPCHRKSFSPIKQMCAIV
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNTSISEIQRFLSMIAFEKELVSEDFSVVAGIDEAGRGPLAGPVVASACILPKGKVFPGV
CCCCHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
NDSKKLSPKQRAQVRDALMQDPEVCFGIGVISVERIDQVNILEATKEAMLQAISSLPISP
CCCCCCCHHHHHHHHHHHHCCCCHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
DILLVDGLYLPHDIPCKKIIQGDAKSASIAAASILAKEHRDDLMLQLHRLYPEYGFDRHK
CEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GYGTSLHVEAIRRYGPSPCHRKSFSPIKQMCAIV
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362