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The map label for this gene is pyk

Identifier: 15835632

GI number: 15835632

Start: 124226

End: 125680

Strand: Direct

Name: pyk

Synonym: CPj0097

Alternate gene names: 15835632

Gene position: 124226-125680 (Clockwise)

Preceding gene: 161353805

Following gene: 15835635

Centisome position: 10.13

GC content: 42.54

Gene sequence:

>1455_bases
ATGATCACACGCACTAAAATTATTTGCACTATAGGGCCAGCAACGAATAGTCCAGAGATGTTAGCAAAACTTCTAGATGC
TGGGATGAACGTAGCAAGATTAAATTTCAGTCATGGGAGTCACGAAACTCATGGACAGGCTATTGGATTTCTCAAGGAGT
TAAGGGAGCAGAAGCGGGTTCCTTTAGCAATTATGCTAGATACTAAGGGGCCTGAAATTCGTTTAGGGAATATTCCTCAG
CCAATTTCGGTTTCTCAGGGACAAAAGCTTCGTCTGGTAAGTAGTGATATCGATGGGAGTGCTGAAGGGGGAGTGTCTCT
CTATCCTAAGGGGATATTTCCCTTTGTTCCTGAGGGTGCTGATGTTTTAATAGATGATGGCTACATTCATGCTGTTGTTG
TCTCTTCAGAGGCTGATTCTTTAGAATTAGAGTTTATGAACAGTGGCCTTCTCAAGTCTCATAAATCTTTGAGTATCCGA
GGTGTTGATGTTGCTCTTCCCTTTATGACAGAGAAAGATATTGCGGATCTTAAGTTTGGGGTAGAGCAGAATATGGATGT
GAATGCTGCATCTTTTGTGCGCTACGGTGAAGATATTGAAACTATGCGCAAGTGTTTAGCAGACTTAGGCAATCCTAAGA
TGCCCATCATTGCAAAAATAGAAAATCGTTTAGGGGTAGAAAATTTCTCTAAGATTGCCAAGCTTGCGGATGGAATTATG
ATTGCTAGAGGAGATTTAGGAATCGAGCTTTCTGTCGTTGAAGTCCCAAATTTGCAAAAGATGATGGCTAAGGTTTCTAG
AGAAACAGGTCACTTCTGTGTGACTGCAACGCAGATGCTAGAATCTATGATTCGCAATGTCTTACCTACACGAGCTGAAG
TCTCTGATATTGCCAATGCAATTTATGATGGTTCTTCAGCAGTGATGTTGTCAGGGGAAACTGCATCTGGAGCCCATCCC
GTGGCTGCCGTGAAAATCATGCGTTCTGTGATTTTAGAAACAGAAAAGAATCTCTCCCATGATTCATTCTTAAAATTAGA
CGATAGCAATAGCGCTCTTCAGGTGTCCCCCTATCTCTCAGCCATTGGATTGGCAGGCATTCAGATTGCAGAAAGGGCAG
ACGCCAAAGCTCTTATTGTTTATACAGAATCAGGAAGTTCTCCGATGTTTCTCTCTAAATATCGTCCGAAATTCCCTATC
ATTGCCGTGACTCCAAGCACTTCTGTTTACTATCGCCTAGCTTTGGAATGGGGGGTCTATCCTATGCTTACCCAGGAAAG
TGATCGCGCTGTATGGAGACATCAGGCCTGTATTTATGGCATAGAACAGGGCATTCTCTCTAATTATGATCGGATTCTTG
TGCTTAGCAGAGGAGCCTGTATGGAAGAAACAAATAATCTTACCCTGACAATAGTGAATGATATTTTGACTGGGTCGGAA
TTTCCTGAAACCTAG

Upstream 100 bases:

>100_bases
GTTTGAGTTAATAAAGAGAATTTTTTAATATTTTTTCAAAAAAGAATATAAAATATTTCATTATACCATGAGTTTTTCAT
TGAATAGACAATAGGACAGT

Downstream 100 bases:

>100_bases
AATTTTTGCTTTAAAATCCAGGACTTCGCAAATTTTTCGAGAATATACAGATGTTTTCGTAAATATGAATTAGGGCTTTT
ACTCCACTGTAAGTCAGGGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MITRTKIICTIGPATNSPEMLAKLLDAGMNVARLNFSHGSHETHGQAIGFLKELREQKRVPLAIMLDTKGPEIRLGNIPQ
PISVSQGQKLRLVSSDIDGSAEGGVSLYPKGIFPFVPEGADVLIDDGYIHAVVVSSEADSLELEFMNSGLLKSHKSLSIR
GVDVALPFMTEKDIADLKFGVEQNMDVNAASFVRYGEDIETMRKCLADLGNPKMPIIAKIENRLGVENFSKIAKLADGIM
IARGDLGIELSVVEVPNLQKMMAKVSRETGHFCVTATQMLESMIRNVLPTRAEVSDIANAIYDGSSAVMLSGETASGAHP
VAAVKIMRSVILETEKNLSHDSFLKLDDSNSALQVSPYLSAIGLAGIQIAERADAKALIVYTESGSSPMFLSKYRPKFPI
IAVTPSTSVYYRLALEWGVYPMLTQESDRAVWRHQACIYGIEQGILSNYDRILVLSRGACMEETNNLTLTIVNDILTGSE
FPET

Sequences:

>Translated_484_residues
MITRTKIICTIGPATNSPEMLAKLLDAGMNVARLNFSHGSHETHGQAIGFLKELREQKRVPLAIMLDTKGPEIRLGNIPQ
PISVSQGQKLRLVSSDIDGSAEGGVSLYPKGIFPFVPEGADVLIDDGYIHAVVVSSEADSLELEFMNSGLLKSHKSLSIR
GVDVALPFMTEKDIADLKFGVEQNMDVNAASFVRYGEDIETMRKCLADLGNPKMPIIAKIENRLGVENFSKIAKLADGIM
IARGDLGIELSVVEVPNLQKMMAKVSRETGHFCVTATQMLESMIRNVLPTRAEVSDIANAIYDGSSAVMLSGETASGAHP
VAAVKIMRSVILETEKNLSHDSFLKLDDSNSALQVSPYLSAIGLAGIQIAERADAKALIVYTESGSSPMFLSKYRPKFPI
IAVTPSTSVYYRLALEWGVYPMLTQESDRAVWRHQACIYGIEQGILSNYDRILVLSRGACMEETNNLTLTIVNDILTGSE
FPET
>Mature_484_residues
MITRTKIICTIGPATNSPEMLAKLLDAGMNVARLNFSHGSHETHGQAIGFLKELREQKRVPLAIMLDTKGPEIRLGNIPQ
PISVSQGQKLRLVSSDIDGSAEGGVSLYPKGIFPFVPEGADVLIDDGYIHAVVVSSEADSLELEFMNSGLLKSHKSLSIR
GVDVALPFMTEKDIADLKFGVEQNMDVNAASFVRYGEDIETMRKCLADLGNPKMPIIAKIENRLGVENFSKIAKLADGIM
IARGDLGIELSVVEVPNLQKMMAKVSRETGHFCVTATQMLESMIRNVLPTRAEVSDIANAIYDGSSAVMLSGETASGAHP
VAAVKIMRSVILETEKNLSHDSFLKLDDSNSALQVSPYLSAIGLAGIQIAERADAKALIVYTESGSSPMFLSKYRPKFPI
IAVTPSTSVYYRLALEWGVYPMLTQESDRAVWRHQACIYGIEQGILSNYDRILVLSRGACMEETNNLTLTIVNDILTGSE
FPET

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=466, Percent_Identity=41.4163090128755, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI10835121, Length=466, Percent_Identity=41.4163090128755, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI33286422, Length=471, Percent_Identity=39.0658174097665, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI33286420, Length=471, Percent_Identity=39.0658174097665, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI33286418, Length=471, Percent_Identity=38.6411889596603, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI310128732, Length=286, Percent_Identity=41.958041958042, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI310128730, Length=286, Percent_Identity=41.958041958042, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI310128736, Length=234, Percent_Identity=41.8803418803419, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI310128734, Length=234, Percent_Identity=41.8803418803419, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=40, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1787965, Length=428, Percent_Identity=42.0560747663551, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=33.195020746888, Blast_Score=216, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17544584, Length=435, Percent_Identity=39.5402298850575, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=472, Percent_Identity=38.9830508474576, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=472, Percent_Identity=38.9830508474576, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=472, Percent_Identity=38.9830508474576, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=472, Percent_Identity=38.9830508474576, Blast_Score=275, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=463, Percent_Identity=39.0928725701944, Blast_Score=282, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6324923, Length=497, Percent_Identity=37.2233400402414, Blast_Score=270, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24648964, Length=470, Percent_Identity=39.1489361702128, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI28571814, Length=470, Percent_Identity=39.1489361702128, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=39.3719806763285, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24581235, Length=429, Percent_Identity=31.7016317016317, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24646914, Length=268, Percent_Identity=35.0746268656716, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52681; Mature: 52681

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRTKIICTIGPATNSPEMLAKLLDAGMNVARLNFSHGSHETHGQAIGFLKELREQKRV
CCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
PLAIMLDTKGPEIRLGNIPQPISVSQGQKLRLVSSDIDGSAEGGVSLYPKGIFPFVPEGA
CEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCC
DVLIDDGYIHAVVVSSEADSLELEFMNSGLLKSHKSLSIRGVDVALPFMTEKDIADLKFG
EEEEECCEEEEEEEECCCCCEEEEECCCCHHHCCCCEEEEEEEEEECCCCCCCHHHHHCC
VEQNMDVNAASFVRYGEDIETMRKCLADLGNPKMPIIAKIENRLGVENFSKIAKLADGIM
CCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHCCEE
IARGDLGIELSVVEVPNLQKMMAKVSRETGHFCVTATQMLESMIRNVLPTRAEVSDIANA
EEECCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHH
IYDGSSAVMLSGETASGAHPVAAVKIMRSVILETEKNLSHDSFLKLDDSNSALQVSPYLS
HCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECHHHH
AIGLAGIQIAERADAKALIVYTESGSSPMFLSKYRPKFPIIAVTPSTSVYYRLALEWGVY
HHHHCCEEEHHCCCCCEEEEEECCCCCCEEECCCCCCCCEEEECCCCCEEEEEEEECCCC
PMLTQESDRAVWRHQACIYGIEQGILSNYDRILVLSRGACMEETNNLTLTIVNDILTGSE
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEHHHCCCC
FPET
CCCC
>Mature Secondary Structure
MITRTKIICTIGPATNSPEMLAKLLDAGMNVARLNFSHGSHETHGQAIGFLKELREQKRV
CCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
PLAIMLDTKGPEIRLGNIPQPISVSQGQKLRLVSSDIDGSAEGGVSLYPKGIFPFVPEGA
CEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCC
DVLIDDGYIHAVVVSSEADSLELEFMNSGLLKSHKSLSIRGVDVALPFMTEKDIADLKFG
EEEEECCEEEEEEEECCCCCEEEEECCCCHHHCCCCEEEEEEEEEECCCCCCCHHHHHCC
VEQNMDVNAASFVRYGEDIETMRKCLADLGNPKMPIIAKIENRLGVENFSKIAKLADGIM
CCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHCCEE
IARGDLGIELSVVEVPNLQKMMAKVSRETGHFCVTATQMLESMIRNVLPTRAEVSDIANA
EEECCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHH
IYDGSSAVMLSGETASGAHPVAAVKIMRSVILETEKNLSHDSFLKLDDSNSALQVSPYLS
HCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECHHHH
AIGLAGIQIAERADAKALIVYTESGSSPMFLSKYRPKFPIIAVTPSTSVYYRLALEWGVY
HHHHCCEEEHHCCCCCEEEEEECCCCCCEEECCCCCCCCEEEECCCCCEEEEEEEECCCC
PMLTQESDRAVWRHQACIYGIEQGILSNYDRILVLSRGACMEETNNLTLTIVNDILTGSE
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEEHHHCCCC
FPET
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]