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Identifier: 16752679

GI number: 16752679

Start: 434580

End: 435818

Strand: Direct

Name: Not Available

Synonym: CP0397

Alternate gene names: 16752679

Gene position: 434580-435818 (Clockwise)

Preceding gene: 16752677

Following gene: 16752680

Centisome position: 35.34

GC content: 41.65

Gene sequence:

>1239_bases
ATGCAATCCTGGTTACAATCTTTACAAGAGCGAAATATTTTAGAGAATTTTACCGCAGGTTTGGAATCCGTAGAGGGACC
TATCGCCGCTTATTTAGGATTTGATCCTACCGCACCTGCTCTACATATTGGTCATTGGATTGGGATTTGTTTCTTGAAGA
GACTCGCTGCTCTGGGGATTACCCCCATAGCTTTAGTCGGGGGAGCCACAGGTATGGTTGGAGATCCCTCAGGGAAACAG
AGCGAGAGATCGTTACTTCAGACAAGTGAAGTTTTTGATAACAGTCAAAAGATCACGGCGTGTCTCCAGCGCTATCTTCC
CGGGGTGACTCTTGTAAATAATGCAGACTGGTTGCAGGAGATCTCCCTGATTGATTTCTTAAGGGATATAGGAAAACACT
TTCGTTTAGGCCAAATGCTAGTGAAAGATACAATAAAGCAGCGGGTGCATTCTGATGAAGGAATTAGCTATACCGAGTTT
AGCTATTTAATCCTGCAATCCTATGATTTTTATCACTTATTTAAAAATTATGGCACGATCTTGCAGTGCGGTGGTAGCGA
TCAGTGGGGGAATATTACTTCAGGAATCGATTTTATTCGCCGTAAAGGGTTGGGTCAGGCCTACGGCCTTACCTATCCTT
TATTAACGAATGCTCAGGGGAAAAAAATAGGGAAAACAGAGTCGGGAACTGTATGGCTCGATTCAGATTTAACCTCTCCT
TTTGAGCTGTACCAATACTTACTCCGTTTGCCCGATGATACCATCCCTAAAATTGCTCGTACGTTAACTTTATTGAGCAA
TGAAGAAATTCAAGATATTGATAGGCGTGTACAGACGGATCCAGTTGCAGTGAAGGAATTTGTAGCCCAAGATATCTTAA
GTGCTATTCATGGAGATCTAGGGCTTGAAGAGGCTCTTTCTGTAACTCGTAGCATGCATCCAGGGAATCTTTCATCCTTA
TCGGAAAAAGATTTTCATGAATTGTTTGCAGGAGGGATGGGGGCCTCATTGGATAAATCCGAGGTGTTAGGGAAACGTTG
GTTAGACCTATTTCTTGTTTTGGGACTATGTAAATCTAAAGGGGAAATTCGAAGGCTAATTGAACAAAAAGGGGTATATA
TTAATAATGTGCCCATCGCTAATGAGCATAGTGTTTGTGAAGAACAAGACATCTGTTATGGTCACTATGTGTTGTTGGCT
CAAGGTAAAAAACGAAAGCTTGTTCTATATTTAAATTAG

Upstream 100 bases:

>100_bases
GTACAATCTTTTCTTTATTGTTAGATAGGTGGTGCCTTGCATATAAGCTTTAGGATCGATAAGATAGAGCTTGGCAATTC
TTAATTATGTACGATCACTC

Downstream 100 bases:

>100_bases
TTTAAGGAGGCTAGGTAGCTTTGCAAACGAATATTGGTCTTATTGGCTTAGCTGTCATGGGGAAAAATCTTGTCTTAAAC
ATGATAGATCATGGTTTTTC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGATGMVGDPSGKQ
SERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEF
SYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGTVWLDSDLTSP
FELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTRSMHPGNLSSL
SEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLA
QGKKRKLVLYLN

Sequences:

>Translated_412_residues
MQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGATGMVGDPSGKQ
SERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEF
SYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGTVWLDSDLTSP
FELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTRSMHPGNLSSL
SEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLA
QGKKRKLVLYLN
>Mature_412_residues
MQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGITPIALVGGATGMVGDPSGKQ
SERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQEISLIDFLRDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEF
SYLILQSYDFYHLFKNYGTILQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGTVWLDSDLTSP
FELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDLGLEEALSVTRSMHPGNLSSL
SEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSKGEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLA
QGKKRKLVLYLN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=404, Percent_Identity=36.1386138613861, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1787925, Length=414, Percent_Identity=40.5797101449275, Blast_Score=297, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI25145802, Length=319, Percent_Identity=40.4388714733542, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI25145805, Length=252, Percent_Identity=38.0952380952381, Blast_Score=154, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6325160, Length=381, Percent_Identity=32.0209973753281, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI20130383, Length=386, Percent_Identity=35.4922279792746, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CHLPN (Q9Z8I2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   D72088
- PIR:   G81581
- PIR:   G86535
- RefSeq:   NP_224561.1
- RefSeq:   NP_300418.1
- RefSeq:   NP_444946.1
- RefSeq:   NP_876644.1
- ProteinModelPortal:   Q9Z8I2
- SMR:   Q9Z8I2
- GeneID:   1467051
- GeneID:   895054
- GeneID:   919130
- GeneID:   962834
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0397
- KEGG:   cpn:CPn0361
- KEGG:   cpt:CpB0370
- TIGR:   CP_0397
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- PhylomeDB:   Q9Z8I2
- ProtClustDB:   PRK05912
- BioCyc:   CPNE115711:CP_0397-MONOMER
- BioCyc:   CPNE115713:CPN0361-MONOMER
- BioCyc:   CPNE138677:CPJ0361-MONOMER
- BioCyc:   CPNE182082:CPB0370-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45915; Mature: 45915

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 31-31 BINDING 162-162 BINDING 166-166 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
TPIALVGGATGMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQE
CHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCHHHHHH
ISLIDFLRDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEFSYLILQSYDFYHLFKNYGTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCE
LQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGTVWLDSDLTSP
EECCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCH
FELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDL
HHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GLEEALSVTRSMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSK
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCH
GEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLAQGKKRKLVLYLN
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEC
>Mature Secondary Structure
MQSWLQSLQERNILENFTAGLESVEGPIAAYLGFDPTAPALHIGHWIGICFLKRLAALGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
TPIALVGGATGMVGDPSGKQSERSLLQTSEVFDNSQKITACLQRYLPGVTLVNNADWLQE
CHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCHHHHHH
ISLIDFLRDIGKHFRLGQMLVKDTIKQRVHSDEGISYTEFSYLILQSYDFYHLFKNYGTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCE
LQCGGSDQWGNITSGIDFIRRKGLGQAYGLTYPLLTNAQGKKIGKTESGTVWLDSDLTSP
EECCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCH
FELYQYLLRLPDDTIPKIARTLTLLSNEEIQDIDRRVQTDPVAVKEFVAQDILSAIHGDL
HHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GLEEALSVTRSMHPGNLSSLSEKDFHELFAGGMGASLDKSEVLGKRWLDLFLVLGLCKSK
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCH
GEIRRLIEQKGVYINNVPIANEHSVCEEQDICYGHYVLLAQGKKRKLVLYLN
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362