Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 16752414

GI number: 16752414

Start: 121918

End: 123093

Strand: Direct

Name: Not Available

Synonym: CP0121

Alternate gene names: 16752414

Gene position: 121918-123093 (Clockwise)

Preceding gene: 16752413

Following gene: 16752415

Centisome position: 9.91

GC content: 37.16

Gene sequence:

>1176_bases
TTGCCGGCCAAGAAAAAGGCGCAGAGTGTAGTTTTAGGGAAGGAGAAAGGGATGTCAGATAACGCACACAATTTACTTTA
TGATAAGTTTGAACTGCCTGAAGCAGTTAAAATGTTGCCTGTTGAAGGGCTTCCAATAGACAAACATGCTCGCTTCATTG
CTGAGCCTCTAGAAAGAGGAATGGGCCACACTTTAGGAAATGCTCTAAGACGTGCTTTGCTTATTGGTTTAGAAGCTCCA
GCTATTATCTCGTTTGCTATGACAGGCGTACTTCATGAATATATGGCAATCGAAGGGGTTATTGAGGATGTAACTAACAT
CATTCTGAATTTAAAAGGTGCCCTATTAAAAAAGTACCCCATGCAGGATAGTTCTTTAGGAAGAACCACTCAGGTCTTAA
AAGCTTCAATTTCTATAGATGCTTCCGATTTAGCCGCAGCTAATGGACAAAAAGAAGTGACTCTACAAGATCTATTGCAA
GAAGGGGACTTTGAAGCCGTTAACCCAGATCAAGTCATTTTTACTGTTACTCAACCCATACAGTTGGAAGTCGTTCTACG
AATTGCTTTTGGTAGGGGATATACACCTTCTGAAAGGATTGTTTTGGAAGATAAGGGTGTTTATGAAATCGTTTTGGATG
CAGCCTTTTCTCCAGTCACTTTAGTCAACTACTTTGTAGAAGATACACGGGTGGGTCAGGATACAGATTTTGACCGTTTA
GTTTTAATAGTTGAAACAGATGGAAGAGTGACTCCGAAAGAAGCTCTGGCTTTTTCAACTCAAATTTTGACTAAACATTT
TTCCATTTTTGAAAATATGGATGAGAAGAAAATCGTATTTGAAGAAGCTATTTCTATTGAGAAAGAAAACAAAGATGATA
TTCTTCATAAGTTAATTTTAGGAATTAATGAAATAGAACTCTCAGTCAGATCAACAAATTGTTTGTCTAATGCAAATATT
GAGACTATTGGCGAACTTGTTATTATGCCTGAACCTCGATTGCTACAATTCAGAAATTTTGGAAAGAAATCACTGTGTGA
GATCAAGAATAAATTGAAAGAAATGAAGCTTGAATTAGGAATGGACCTAACGCAATTTGGCGTAGGTTTAGATAACGTAA
AAGAAAAAATGAAGTGGTATGCCGAAAAGATTCGGGCTAAAAATATAAAGGGATAG

Upstream 100 bases:

>100_bases
AGGGTACTGGAGCTGGGAGAGAGTCTGCTGTTCGTGCTTTGATATCTGCTGGTTTGGTAGTTTCTGTAATCCGTGATGAA
ACTCCTGTTCCTCATAATGG

Downstream 100 bases:

>100_bases
TAAGTAATGCAACACGCTAGAAAAAAATTTAGAGTTGGTCGTACTTCCTCGCATAATCGCTGTATGTTAGCTAACATGTT
AAAGTCTCTAATTCATTATG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MPAKKKAQSVVLGKEKGMSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAP
AIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQ
EGDFEAVNPDQVIFTVTQPIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRL
VLIVETDGRVTPKEALAFSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANI
ETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG

Sequences:

>Translated_391_residues
MPAKKKAQSVVLGKEKGMSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAP
AIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQ
EGDFEAVNPDQVIFTVTQPIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRL
VLIVETDGRVTPKEALAFSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANI
ETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG
>Mature_390_residues
PAKKKAQSVVLGKEKGMSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERGMGHTLGNALRRALLIGLEAPA
IISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYPMQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQE
GDFEAVNPDQVIFTVTQPIQLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRLV
LIVETDGRVTPKEALAFSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLILGINEIELSVRSTNCLSNANIE
TIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=38.5093167701863, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 43656; Mature: 43525

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKKKAQSVVLGKEKGMSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERG
CCCHHHHHHHEEECCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHC
MGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYP
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQPI
CCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCC
QLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRL
EEEEEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEE
VLIVETDGRVTPKEALAFSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLIL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
GINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELG
CCCCEEEEEECCCCCCCCCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHC
MDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG
CCHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PAKKKAQSVVLGKEKGMSDNAHNLLYDKFELPEAVKMLPVEGLPIDKHARFIAEPLERG
CCHHHHHHHEEECCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHC
MGHTLGNALRRALLIGLEAPAIISFAMTGVLHEYMAIEGVIEDVTNIILNLKGALLKKYP
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MQDSSLGRTTQVLKASISIDASDLAAANGQKEVTLQDLLQEGDFEAVNPDQVIFTVTQPI
CCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCC
QLEVVLRIAFGRGYTPSERIVLEDKGVYEIVLDAAFSPVTLVNYFVEDTRVGQDTDFDRL
EEEEEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEE
VLIVETDGRVTPKEALAFSTQILTKHFSIFENMDEKKIVFEEAISIEKENKDDILHKLIL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
GINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELG
CCCCEEEEEECCCCCCCCCHHHHCCEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHC
MDLTQFGVGLDNVKEKMKWYAEKIRAKNIKG
CCHHHHCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]