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Identifier: 16752380

GI number: 16752380

Start: 101258

End: 101728

Strand: Direct

Name: Not Available

Synonym: CP0087

Alternate gene names: 16752380

Gene position: 101258-101728 (Clockwise)

Preceding gene: 16752379

Following gene: 16752382

Centisome position: 8.23

GC content: 40.13

Gene sequence:

>471_bases
ATGAGAGTAGTTCTTCATTGTCCTGATATTCCACAGAATACTGGAAATATAGGTAGAACTTGTGTAGCCCTAGGCGCCGA
ACTCATTTTGGTTCGGCCCTTGGGCTTTTCTTTAGCCGATAAATTTGTCAAACGTGCAGGGATGGACTACTGGGACAAAC
TCCAATTGACAGTAGTGGATTCTATAGAAGAAGCACTACATGATGTCCCTGAAGATCAGATTTTTTGTCTTTCTACCAAA
GGTTCAGCATCTTATACTGAATTTTCCTTGCCATCTTCAGGCACCTACGTCTTTGGATCTGAATCCAAAGGTCTTCCAAA
AGAGATCTTAAAAAAATATTATAAAAATTGCCTGCGCATTCCCATGCAACAAGACATCCGTTCTCTAAATTTAGCTACCA
GTGTAGGGATCGTTTTATATGAAGTGGTAAGGCAGAAAACCGTAGCATTGCAAAAGAATCCTACGGTTTAA

Upstream 100 bases:

>100_bases
GACAACTTCCTCCAAACTCTTTATTAATTTTTGAAATTAACTTGATTCAGGCTTCAGCAGATGAAGTTGCTGCTGTACCC
CAAGAAGGAAATCAAGGTGA

Downstream 100 bases:

>100_bases
TATTGCAGCGTCTTTTTAAGCGTGCTTATTGATAAGATTGGTTAGGAATTCTTTATCCTTAAGACCTACGACCCGAGCCA
CCTCGTTCCCATCCTTAAAA

Product: spoU rRNA methylase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRVVLHCPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDKLQLTVVDSIEEALHDVPEDQIFCLSTK
GSASYTEFSLPSSGTYVFGSESKGLPKEILKKYYKNCLRIPMQQDIRSLNLATSVGIVLYEVVRQKTVALQKNPTV

Sequences:

>Translated_156_residues
MRVVLHCPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDKLQLTVVDSIEEALHDVPEDQIFCLSTK
GSASYTEFSLPSSGTYVFGSESKGLPKEILKKYYKNCLRIPMQQDIRSLNLATSVGIVLYEVVRQKTVALQKNPTV
>Mature_156_residues
MRVVLHCPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDKLQLTVVDSIEEALHDVPEDQIFCLSTK
GSASYTEFSLPSSGTYVFGSESKGLPKEILKKYYKNCLRIPMQQDIRSLNLATSVGIVLYEVVRQKTVALQKNPTV

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=146, Percent_Identity=37.6712328767123, Blast_Score=107, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17337; Mature: 17337

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVLHCPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDKLQLTVVD
CEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHH
SIEEALHDVPEDQIFCLSTKGSASYTEFSLPSSGTYVFGSESKGLPKEILKKYYKNCLRI
HHHHHHHHCCCCCEEEEECCCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCC
PMQQDIRSLNLATSVGIVLYEVVRQKTVALQKNPTV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRVVLHCPDIPQNTGNIGRTCVALGAELILVRPLGFSLADKFVKRAGMDYWDKLQLTVVD
CEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHH
SIEEALHDVPEDQIFCLSTKGSASYTEFSLPSSGTYVFGSESKGLPKEILKKYYKNCLRI
HHHHHHHHCCCCCEEEEECCCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCC
PMQQDIRSLNLATSVGIVLYEVVRQKTVALQKNPTV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]