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The map label for this gene is aspS

Identifier: 16752378

GI number: 16752378

Start: 98662

End: 100416

Strand: Direct

Name: aspS

Synonym: CP0085

Alternate gene names: 16752378

Gene position: 98662-100416 (Clockwise)

Preceding gene: 16752377

Following gene: 16752379

Centisome position: 8.02

GC content: 40.85

Gene sequence:

>1755_bases
ATGAAATACAGAACACACCGTTGTAATGAACTCACAAGTAATCATATAGGTGAGAATGTTCAATTGGCAGGATGGGTGCA
CCGTTATCGTAATCATGGGGGTGTTGTCTTCATAGATTTGCGAGATCGTTTTGGTATTACTCAAATTGTGTGCCGTGAAG
ATGAGCAACCAGAACTGCACCAACGTTTGGATGCTGTGCGTTCTGAATGGGTTCTTTCTGTACGAGGGAAAGTATGTCCA
CGTCTTGCAGGAATGGAAAATCCTAACTTAGCAACAGGACATATTGAAGTTGAAGTTGCAAGCTTTGAAGTGCTATCCAA
GTCGCAAAATCTCCCTTTTTCCATTGCCGATGACCACATCAATGTGAATGAGGAGCTGCGTTTAGAGTATCGTTATCTAG
ATATGCGTCGCGGGGATATTATTGAGAAGTTGCTTTGTCGTCATCAGGTCATGCTTGCTTGCCGCAACTTTATGGATGCT
CAAGGATTCACAGAAATCGTGACCCCTGTATTAGGAAAATCCACTCCTGAAGGTGCTAGAGATTATCTTGTTCCCTCTAG
AATCTATCCTGGAAAATTTTATGCTCTACCGCAATCACCACAACTTTTTAAGCAGCTTTTAATGGTTGGAGGCTTGGATC
GTTATTTCCAAATCGCCACGTGCTTTAGAGACGAAGATTTGCGAGCAGATCGTCAACCTGAATTTGCTCAAATAGATATT
GAAATGAGCTTTGGGGATACCCAGGACCTACTTCCCATTATAGAACAATTGGTCGCAACACTGTTTGCTACGCAAGGTAT
AGAGATTCCTTTGCCTTTGGCTAAAATGACCTATCAAGAAGCTAAGGATTCCTATGGTACAGATAAACCAGATTTAAGAT
TCGATCTTAAATTAAAAGATTGTCGAGACTATGCAAAACGTTCCTCATTCTCTATTTTCTTAGATCAGTTGGCTCACGGA
GGTACGATCAAAGGTTTTTGTGTTCCAGGTGGGGCTACTATGTCCCGCAAGCAACTCGATGGTTATACCGAGTTTGTAAA
GCGTTATGGGGCTATGGGGCTTGTCTGGATTAAAAATCAAGAGGGGAAAGTTGCTTCCAATATTGCTAAATTTATGGATG
AAGAGGTTTTCCACGAACTCTTTGCTTATTTTGATGCCAAAGATCAGGATATTTTGCTCTTGATAGCAGCTCCTGAATCA
GTAGCGAATCAGTCTCTCGATCATCTGCGTAGATTGATCGCAAAAGAGCGCGAATTGTATAGCGACAATCAATATAACTT
TGTTTGGATTACAGATTTCCCTCTTTTCTCTTTAGAAGATGGAAAAATTGTGGCGGAACACCATCCGTTTACAGCACCTT
TGGAGGAGGATATTCCTCTGTTAGAAACAGATCCTCTAGCAGTGCGTTCGTCAAGCTATGACTTGGTTTTAAATGGATAT
GAAATTGCTTCGGGATCTCAAAGAATTCATAACCCAGACTTGCAAAGTCAAATATTTACTATTTTAAAAATAAGTCCTGA
GAGCATCCAAGAAAAATTTGGATTCTTTATCAAAGCTTTGAGTTTTGGAACCCCACCTCATCTAGGGATTGCCTTAGGGT
TAGATCGATTGGTTATGGTTTTAACAGCAGCAGAGAGCATTCGCGAGGTCATCGCGTTTCCTAAAACACAGAAAGCATCG
GATCTTATGATGAATGCTCCCTCAGAAATTATGTCTTCCCAATTAAAAGAGCTAAGTATTAAGGTAGCTTTTTGA

Upstream 100 bases:

>100_bases
GGTGAGCGAGAGTTAATTTCTCAACAGTTAGTTATTAAAAATATGTCTCTGCGTAAAGAATTCTTTGGAACAAAAGAAGA
AGTAGAGCAAAGGTTGTTAT

Downstream 100 bases:

>100_bases
TATAAGTTGTAATTGCTTCGGGCATGTAGCATAGTCGTTGCCTTAAGATCAAAGATAGGAGAAGGAAAATGAACAGACGG
TGGAATTTAGTTTTAGCAAC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MKYRTHRCNELTSNHIGENVQLAGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEQPELHQRLDAVRSEWVLSVRGKVCP
RLAGMENPNLATGHIEVEVASFEVLSKSQNLPFSIADDHINVNEELRLEYRYLDMRRGDIIEKLLCRHQVMLACRNFMDA
QGFTEIVTPVLGKSTPEGARDYLVPSRIYPGKFYALPQSPQLFKQLLMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
EMSFGDTQDLLPIIEQLVATLFATQGIEIPLPLAKMTYQEAKDSYGTDKPDLRFDLKLKDCRDYAKRSSFSIFLDQLAHG
GTIKGFCVPGGATMSRKQLDGYTEFVKRYGAMGLVWIKNQEGKVASNIAKFMDEEVFHELFAYFDAKDQDILLLIAAPES
VANQSLDHLRRLIAKERELYSDNQYNFVWITDFPLFSLEDGKIVAEHHPFTAPLEEDIPLLETDPLAVRSSSYDLVLNGY
EIASGSQRIHNPDLQSQIFTILKISPESIQEKFGFFIKALSFGTPPHLGIALGLDRLVMVLTAAESIREVIAFPKTQKAS
DLMMNAPSEIMSSQLKELSIKVAF

Sequences:

>Translated_584_residues
MKYRTHRCNELTSNHIGENVQLAGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEQPELHQRLDAVRSEWVLSVRGKVCP
RLAGMENPNLATGHIEVEVASFEVLSKSQNLPFSIADDHINVNEELRLEYRYLDMRRGDIIEKLLCRHQVMLACRNFMDA
QGFTEIVTPVLGKSTPEGARDYLVPSRIYPGKFYALPQSPQLFKQLLMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
EMSFGDTQDLLPIIEQLVATLFATQGIEIPLPLAKMTYQEAKDSYGTDKPDLRFDLKLKDCRDYAKRSSFSIFLDQLAHG
GTIKGFCVPGGATMSRKQLDGYTEFVKRYGAMGLVWIKNQEGKVASNIAKFMDEEVFHELFAYFDAKDQDILLLIAAPES
VANQSLDHLRRLIAKERELYSDNQYNFVWITDFPLFSLEDGKIVAEHHPFTAPLEEDIPLLETDPLAVRSSSYDLVLNGY
EIASGSQRIHNPDLQSQIFTILKISPESIQEKFGFFIKALSFGTPPHLGIALGLDRLVMVLTAAESIREVIAFPKTQKAS
DLMMNAPSEIMSSQLKELSIKVAF
>Mature_584_residues
MKYRTHRCNELTSNHIGENVQLAGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEQPELHQRLDAVRSEWVLSVRGKVCP
RLAGMENPNLATGHIEVEVASFEVLSKSQNLPFSIADDHINVNEELRLEYRYLDMRRGDIIEKLLCRHQVMLACRNFMDA
QGFTEIVTPVLGKSTPEGARDYLVPSRIYPGKFYALPQSPQLFKQLLMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
EMSFGDTQDLLPIIEQLVATLFATQGIEIPLPLAKMTYQEAKDSYGTDKPDLRFDLKLKDCRDYAKRSSFSIFLDQLAHG
GTIKGFCVPGGATMSRKQLDGYTEFVKRYGAMGLVWIKNQEGKVASNIAKFMDEEVFHELFAYFDAKDQDILLLIAAPES
VANQSLDHLRRLIAKERELYSDNQYNFVWITDFPLFSLEDGKIVAEHHPFTAPLEEDIPLLETDPLAVRSSSYDLVLNGY
EIASGSQRIHNPDLQSQIFTILKISPESIQEKFGFFIKALSFGTPPHLGIALGLDRLVMVLTAAESIREVIAFPKTQKAS
DLMMNAPSEIMSSQLKELSIKVAF

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=41.9191919191919, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI5031815, Length=257, Percent_Identity=26.8482490272374, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI194272210, Length=257, Percent_Identity=26.8482490272374, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI45439306, Length=254, Percent_Identity=26.3779527559055, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=44.7278911564626, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1790571, Length=304, Percent_Identity=27.6315789473684, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789256, Length=292, Percent_Identity=27.0547945205479, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=33.8333333333333, Blast_Score=309, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=23.9285714285714, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=348, Percent_Identity=27.5862068965517, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994340, Length=256, Percent_Identity=23.046875, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=256, Percent_Identity=23.046875, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=256, Percent_Identity=23.046875, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=642, Percent_Identity=33.1775700934579, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6321807, Length=248, Percent_Identity=26.6129032258064, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=25, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323011, Length=261, Percent_Identity=24.904214559387, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24584738, Length=592, Percent_Identity=37.1621621621622, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24640849, Length=264, Percent_Identity=28.4090909090909, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640851, Length=264, Percent_Identity=28.4090909090909, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17136276, Length=249, Percent_Identity=26.9076305220884, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CHLPN (Q9Z7P2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86573
- PIR:   G72052
- RefSeq:   NP_224858.1
- RefSeq:   NP_300718.1
- RefSeq:   NP_444637.1
- RefSeq:   NP_876960.1
- ProteinModelPortal:   Q9Z7P2
- SMR:   Q9Z7P2
- PHCI-2DPAGE:   Q9Z7P2
- GeneID:   1467367
- GeneID:   895247
- GeneID:   919397
- GeneID:   963774
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0085
- KEGG:   cpn:CPn0662
- KEGG:   cpt:CpB0688
- TIGR:   CP_0085
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q9Z7P2
- ProtClustDB:   PRK00476
- BioCyc:   CPNE115711:CP_0085-MONOMER
- BioCyc:   CPNE115713:CPN0662-MONOMER
- BioCyc:   CPNE138677:CPJ0662-MONOMER
- BioCyc:   CPNE182082:CPB0688-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66346; Mature: 66346

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRTHRCNELTSNHIGENVQLAGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEQPELH
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHHH
QRLDAVRSEWVLSVRGKVCPRLAGMENPNLATGHIEVEVASFEVLSKSQNLPFSIADDHI
HHHHHHHHHHEEECCCCCCHHHCCCCCCCEEECEEEEEEEHHHHHHCCCCCCEEEECCCC
NVNEELRLEYRYLDMRRGDIIEKLLCRHQVMLACRNFMDAQGFTEIVTPVLGKSTPEGAR
CCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
DYLVPSRIYPGKFYALPQSPQLFKQLLMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
HCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFGDTQDLLPIIEQLVATLFATQGIEIPLPLAKMTYQEAKDSYGTDKPDLRFDLKLKD
EEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHH
CRDYAKRSSFSIFLDQLAHGGTIKGFCVPGGATMSRKQLDGYTEFVKRYGAMGLVWIKNQ
HHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECC
EGKVASNIAKFMDEEVFHELFAYFDAKDQDILLLIAAPESVANQSLDHLRRLIAKERELY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHC
SDNQYNFVWITDFPLFSLEDGKIVAEHHPFTAPLEEDIPLLETDPLAVRSSSYDLVLNGY
CCCCEEEEEEECCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECE
EIASGSQRIHNPDLQSQIFTILKISPESIQEKFGFFIKALSFGTPPHLGIALGLDRLVMV
EECCCCCCCCCCCHHHHHEEHEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LTAAESIREVIAFPKTQKASDLMMNAPSEIMSSQLKELSIKVAF
HHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MKYRTHRCNELTSNHIGENVQLAGWVHRYRNHGGVVFIDLRDRFGITQIVCREDEQPELH
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHHH
QRLDAVRSEWVLSVRGKVCPRLAGMENPNLATGHIEVEVASFEVLSKSQNLPFSIADDHI
HHHHHHHHHHEEECCCCCCHHHCCCCCCCEEECEEEEEEEHHHHHHCCCCCCEEEECCCC
NVNEELRLEYRYLDMRRGDIIEKLLCRHQVMLACRNFMDAQGFTEIVTPVLGKSTPEGAR
CCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
DYLVPSRIYPGKFYALPQSPQLFKQLLMVGGLDRYFQIATCFRDEDLRADRQPEFAQIDI
HCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFGDTQDLLPIIEQLVATLFATQGIEIPLPLAKMTYQEAKDSYGTDKPDLRFDLKLKD
EEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHH
CRDYAKRSSFSIFLDQLAHGGTIKGFCVPGGATMSRKQLDGYTEFVKRYGAMGLVWIKNQ
HHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECC
EGKVASNIAKFMDEEVFHELFAYFDAKDQDILLLIAAPESVANQSLDHLRRLIAKERELY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHC
SDNQYNFVWITDFPLFSLEDGKIVAEHHPFTAPLEEDIPLLETDPLAVRSSSYDLVLNGY
CCCCEEEEEEECCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECE
EIASGSQRIHNPDLQSQIFTILKISPESIQEKFGFFIKALSFGTPPHLGIALGLDRLVMV
EECCCCCCCCCCCHHHHHEEHEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LTAAESIREVIAFPKTQKASDLMMNAPSEIMSSQLKELSIKVAF
HHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362