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The map label for this gene is tolB

Identifier: 16752259

GI number: 16752259

Start: 1190378

End: 1191673

Strand: Direct

Name: tolB

Synonym: CP1090

Alternate gene names: 16752259

Gene position: 1190378-1191673 (Clockwise)

Preceding gene: 16752258

Following gene: 16752260

Centisome position: 96.79

GC content: 42.13

Gene sequence:

>1296_bases
ATGTTACGGCAACTATGCTTCCAAGTTTTTTTCTTTTGCTTCGCATCGCTAGTCTATGCTGAAGAATTAGAAGTTGTTGT
CCGTTCCGAACATATCACGCTCCCTATTGAGGTCTCTTGCCAGACCGATACGAAAGATCCAAAAATACAGAAATACCTCA
GCTCGCTAACGGAGATATTTTGCAAGGACATTGCCCTAGGAGATTGTCTACAACCCACAGCGGCTTCTAAAGAATCGTCA
TCTCCTTTAGCAATATCTTTACGGTTGCATGTACCTCAGCTATCTGTAGTGCTTTTACAGTCTTCAAAAACTCCTCAAAC
CTTATGTTCTTTTACTATTTCTCAAAATCTTTCTGTAGATCGTCAAAAAATCCATCACGCTGCTGATACAGTTCATTACG
CCCTCACAGGGATTCCTGGAATCAGTGCTGGGAAAATTGTTTTTGCTCTAAGTTCTTTAGGAAAAGATCAAAAGCTCAAG
CAAGGAGAATTATGGACTACAGATTACGATGGGAAAAACCTCGCCCCTTTAACCACAGAATGTTCGCTCTCTATAACTCC
AAAATGGGTGGGTGTGGGATCAAATTTTCCCTATCTCTATGTTTCGTATAAGTATGGTGTGCCTAAAATTTTTCTTGGTT
CCCTAGAGAACACTGAAGGTAAAAAAGTCCTTCCGTTAAAAGGCAACCAACTCATGCCTACGTTTTCTCCAAGAAAAAAG
CTTTTAGCTTTCGTTGCTGATACGTATGGAAATCCTGATTTATTTATTCAACCGTTCTCACTAACTTCAGGACCTATGGG
TCGCCCACGTCGCCTCCTTAATGAGAATTTCGGGACTCAAGGGAATCCCTCCTTCAACCCTGAAGGATCCCAGCTTGTCT
TTATATCGAACAAAGACGGCCGTCCGCGTCTTTATATTATGTCCCTCGATCCTGAACCCCAAGCACCTCGCTTGCTGACA
AAAAAATACAGAAATAGCAGTTGCCCTGCATGGTCTCCAGATGGTAAAAAAATAGCCTTCTGCTCTGTAATTAAAGGGGT
GCGACAAATTTGTATTTACGATCTCTCCTCTGGAGAGGATTACCAACTCACTACGTCTCCCACAAATAAAGAGAGTCCTT
CTTGGGCTATAGACAGCCGTCATCTTGTCTTTAGTGCGGGGAATGCTGAAGAATCAGAGTTATATTTAATCAGTCTAGTC
ACCAAAAAAACTAACAAAATTGCTATAGGAGTAGGAGAAAAACGGTTCCCCTCCTGGGGTGCTTTCCCTCAGCAACCGAT
AAAGAGAACACTATGA

Upstream 100 bases:

>100_bases
TTTCTCGAAAAATACAAAGTCTCGAAAAATATATCTTTTCATATTAAACTGGTCAGTAATGAATCCTAACGCTCTATCCC
TAGGGTGAGGAGATATCGGC

Downstream 100 bases:

>100_bases
ATATACATTCCCTATGGAAACTTTGTACTTTATTGGCTTTACTTGCATTGCCAGCATGTAGCCTTTCCCCTAATTATGGC
TGGGAGGATTCCTGTAATAC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MLRQLCFQVFFFCFASLVYAEELEVVVRSEHITLPIEVSCQTDTKDPKIQKYLSSLTEIFCKDIALGDCLQPTAASKESS
SPLAISLRLHVPQLSVVLLQSSKTPQTLCSFTISQNLSVDRQKIHHAADTVHYALTGIPGISAGKIVFALSSLGKDQKLK
QGELWTTDYDGKNLAPLTTECSLSITPKWVGVGSNFPYLYVSYKYGVPKIFLGSLENTEGKKVLPLKGNQLMPTFSPRKK
LLAFVADTYGNPDLFIQPFSLTSGPMGRPRRLLNENFGTQGNPSFNPEGSQLVFISNKDGRPRLYIMSLDPEPQAPRLLT
KKYRNSSCPAWSPDGKKIAFCSVIKGVRQICIYDLSSGEDYQLTTSPTNKESPSWAIDSRHLVFSAGNAEESELYLISLV
TKKTNKIAIGVGEKRFPSWGAFPQQPIKRTL

Sequences:

>Translated_431_residues
MLRQLCFQVFFFCFASLVYAEELEVVVRSEHITLPIEVSCQTDTKDPKIQKYLSSLTEIFCKDIALGDCLQPTAASKESS
SPLAISLRLHVPQLSVVLLQSSKTPQTLCSFTISQNLSVDRQKIHHAADTVHYALTGIPGISAGKIVFALSSLGKDQKLK
QGELWTTDYDGKNLAPLTTECSLSITPKWVGVGSNFPYLYVSYKYGVPKIFLGSLENTEGKKVLPLKGNQLMPTFSPRKK
LLAFVADTYGNPDLFIQPFSLTSGPMGRPRRLLNENFGTQGNPSFNPEGSQLVFISNKDGRPRLYIMSLDPEPQAPRLLT
KKYRNSSCPAWSPDGKKIAFCSVIKGVRQICIYDLSSGEDYQLTTSPTNKESPSWAIDSRHLVFSAGNAEESELYLISLV
TKKTNKIAIGVGEKRFPSWGAFPQQPIKRTL
>Mature_431_residues
MLRQLCFQVFFFCFASLVYAEELEVVVRSEHITLPIEVSCQTDTKDPKIQKYLSSLTEIFCKDIALGDCLQPTAASKESS
SPLAISLRLHVPQLSVVLLQSSKTPQTLCSFTISQNLSVDRQKIHHAADTVHYALTGIPGISAGKIVFALSSLGKDQKLK
QGELWTTDYDGKNLAPLTTECSLSITPKWVGVGSNFPYLYVSYKYGVPKIFLGSLENTEGKKVLPLKGNQLMPTFSPRKK
LLAFVADTYGNPDLFIQPFSLTSGPMGRPRRLLNENFGTQGNPSFNPEGSQLVFISNKDGRPRLYIMSLDPEPQAPRLLT
KKYRNSSCPAWSPDGKKIAFCSVIKGVRQICIYDLSSGEDYQLTTSPTNKESPSWAIDSRHLVFSAGNAEESELYLISLV
TKKTNKIAIGVGEKRFPSWGAFPQQPIKRTL

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=299, Percent_Identity=26.4214046822742, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_CHLPN (Q9Z7C4)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72037
- PIR:   D86588
- RefSeq:   NP_224977.1
- RefSeq:   NP_300839.1
- RefSeq:   NP_445627.1
- RefSeq:   NP_877082.1
- ProteinModelPortal:   Q9Z7C4
- GeneID:   1467489
- GeneID:   895117
- GeneID:   919553
- GeneID:   963578
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP1090
- KEGG:   cpn:CPn0782
- KEGG:   cpt:CpB0810
- TIGR:   CP_1090
- HOGENOM:   HBG415163
- OMA:   LKDNTES
- PhylomeDB:   Q9Z7C4
- ProtClustDB:   PRK01029
- BioCyc:   CPNE115711:CP_1090-MONOMER
- BioCyc:   CPNE115713:CPN0782-MONOMER
- BioCyc:   CPNE138677:CPJ0782-MONOMER
- BioCyc:   CPNE182082:CPB0810-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR015943
- Gene3D:   G3DSA:2.130.10.10

Pfam domain/function: PF07676 PD40

EC number: NA

Molecular weight: Translated: 47761; Mature: 47761

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQLCFQVFFFCFASLVYAEELEVVVRSEHITLPIEVSCQTDTKDPKIQKYLSSLTEIF
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHHH
CKDIALGDCLQPTAASKESSSPLAISLRLHVPQLSVVLLQSSKTPQTLCSFTISQNLSVD
HHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCHHHHHEEHHHCCCCHH
RQKIHHAADTVHYALTGIPGISAGKIVFALSSLGKDQKLKQGELWTTDYDGKNLAPLTTE
HHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEECE
CSLSITPKWVGVGSNFPYLYVSYKYGVPKIFLGSLENTEGKKVLPLKGNQLMPTFSPRKK
EEEEECCEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEECCCEECCCCCHHHH
LLAFVADTYGNPDLFIQPFSLTSGPMGRPRRLLNENFGTQGNPSFNPEGSQLVFISNKDG
HHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCC
RPRLYIMSLDPEPQAPRLLTKKYRNSSCPAWSPDGKKIAFCSVIKGVRQICIYDLSSGED
CCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHEEEEECCCCCC
YQLTTSPTNKESPSWAIDSRHLVFSAGNAEESELYLISLVTKKTNKIAIGVGEKRFPSWG
EEEEECCCCCCCCCEEECCCEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCC
AFPQQPIKRTL
CCCCCHHHHCC
>Mature Secondary Structure
MLRQLCFQVFFFCFASLVYAEELEVVVRSEHITLPIEVSCQTDTKDPKIQKYLSSLTEIF
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHHH
CKDIALGDCLQPTAASKESSSPLAISLRLHVPQLSVVLLQSSKTPQTLCSFTISQNLSVD
HHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCHHHHHEEHHHCCCCHH
RQKIHHAADTVHYALTGIPGISAGKIVFALSSLGKDQKLKQGELWTTDYDGKNLAPLTTE
HHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEECE
CSLSITPKWVGVGSNFPYLYVSYKYGVPKIFLGSLENTEGKKVLPLKGNQLMPTFSPRKK
EEEEECCEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEECCCEECCCCCHHHH
LLAFVADTYGNPDLFIQPFSLTSGPMGRPRRLLNENFGTQGNPSFNPEGSQLVFISNKDG
HHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCC
RPRLYIMSLDPEPQAPRLLTKKYRNSSCPAWSPDGKKIAFCSVIKGVRQICIYDLSSGED
CCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHEEEEECCCCCC
YQLTTSPTNKESPSWAIDSRHLVFSAGNAEESELYLISLVTKKTNKIAIGVGEKRFPSWG
EEEEECCCCCCCCCEEECCCEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCC
AFPQQPIKRTL
CCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362