Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 13357846

GI number: 13357846

Start: 322935

End: 324671

Strand: Direct

Name: aspS

Synonym: UU286

Alternate gene names: 13357846

Gene position: 322935-324671 (Clockwise)

Preceding gene: 13357845

Following gene: 13357847

Centisome position: 42.96

GC content: 27.35

Gene sequence:

>1737_bases
ATGAAAGTTTATTGTGGTTATATTGGTGAAGAACATTTAGAAAAAAATGTTATTTTAAATGGTTGAGTAAAAAAAGTTCG
TAAAATGGGTAATTTAGTTTTTGTTGACCTAAAAGATCGATTTGGAATTGTACAAATTTTTGCAACAAAAAGTGATGGTG
TTTTTAATGAATTAACACAACTTTCACGTGAGGATGTAATTAATGTTGAAGGTTTAGTTTTGTTGAGAAAAAACCCTAAT
AATGATTTAAAAACAGGTAGATTTGAAATTCATGTCAAAAAAATACTTATTTATTCAAAAGCAAAAACACCACCATTAAT
TATTGAAGATGAAACTGATGCAAATGAGGAAATTCGTTTTAGATATCGTTATTTAGATTTACGTCGTGATGTTAATTTAA
AAATTTTTGAATTGCGAAGTAAAGTTTACCAAGCTTTTAGAAATTACTTATATTCACAAGACTTTATTGAAACTGAAACT
CCTATTTTAGCAAAACCAACGCCAGAAGGTGCACGAGATTTTTATGTGCCAACAAGAACTAGAAAATTTTATGCTTTACC
ACAATCACCTCAAACTTTTAAACAATTATTAATGGTTGCGGGTTTTCAAAAATATTTTCAAATTACAAAATGTTTTCGTG
ATGAAGATTTAAGAAGCGATCGTCAACCAGAATTTACTCAAGTTGATATAGAATTATCATTTGCTGATGAAATAGAAATT
CAGACATTAATTGAAAATTTATTAAAATATGTTTTTAAACAAACCATTAATGTTGATTTAACAACTCCTTTTATGCGAAT
GAGTTATGAGCAAGCGATAAATGATTATGGTAGTGATAAACCAGATTTACGTTTTGATTTGAAAATTAAAACACTTAACA
CATATTTTGAAAATTCAAAAACATTATTTTTTCAAAAAGCACTTTTAAATAATCAAAGTATTCGTGCAATTTTAGTACCT
AATATTAATTTAAATAAAAAACAAGTACAAACTCTTGAAAAGTTTGCCAAAGACAAAGGTGCTAAAGGTTTAAGTTGAAT
AAGTATTAAAGATGAAAAAATTATAGATGGTTCGTTATTATCAATTCAAGAAGATCATATAATTTATAAAACGATTTTTA
AAGACTTTAATTTATCAACTGGATCAATTTTATTAGTTGCTGATACTTTTGATATTGCATCTCAAGCATTAGGTTTAGTA
CGTATAAATTTAGCATCTATTCTTAATTTAAAAAAACCCAATATTTTTAAATTTGTTTGAATTATTGATTGACCATTATA
TGAATATGATAATGAGGCTCAACGTTTTGTTGCTGCACACCATCCTTTCACAATGCCAACTTTAGAAACATTAAATACTT
TTGATGTAAATAAAAAAGACGCTAGAGGTCGTAGTTATGATATTGTTTTAAATGGTTATGAATTAGGTGGAGGATCAGTT
CGGATTATAGACCAACAAATTCAACGCCGTATGTTTAAATCAATTAATATGAGTGATGAAGAGGCTAATTTAAAATTCGG
TTTTTTACTAACTGCATTCGAATATGGTGTTCCGCCACATTGTGGAATTGCTTTGGGTTTAGATCGTTTGATGATGATTT
TAGTGAATAGTGAATATATTCGCGATGTTGTTGCATTTCCTAAGAATAACAACGGCGTTGATATGATGTTAGACGCACCA
TCTAACATGAATGATGAAGATTTAAAAGAATTAGGTTTAAAAATAAAAAATGATTAA

Upstream 100 bases:

>100_bases
AAAAAGAATGATCAGATAAAAAAATAATTTTAAAAGATTTACTTAATTTTAAACAAGATTTATTAAGTTTAGATGAAATT
AAAGGAATAAGGTAATAAAA

Downstream 100 bases:

>100_bases
TAAAAAGATAAAGATTAACCCAAATATAACATATTTTAAACAAAAAATTTCTAATTATCATGAGCATTTGTTAGTTTGAA
ATGATTTATATGATAAATTA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKVYCGYIGEEHLEKNVILNGWVKKVRKMGNLVFVDLKDRFGIVQIFATKSDGVFNELTQLSREDVINVEGLVLLRKNPN
NDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFRYRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETET
PILAKPTPEGARDFYVPTRTRKFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEI
QTLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKTLFFQKALLNNQSIRAILVP
NINLNKKQVQTLEKFAKDKGAKGLSWISIKDEKIIDGSLLSIQEDHIIYKTIFKDFNLSTGSILLVADTFDIASQALGLV
RINLASILNLKKPNIFKFVWIIDWPLYEYDNEAQRFVAAHHPFTMPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSV
RIIDQQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVVAFPKNNNGVDMMLDAP
SNMNDEDLKELGLKIKND

Sequences:

>Translated_578_residues
MKVYCGYIGEEHLEKNVILNG*VKKVRKMGNLVFVDLKDRFGIVQIFATKSDGVFNELTQLSREDVINVEGLVLLRKNPN
NDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFRYRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETET
PILAKPTPEGARDFYVPTRTRKFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEI
QTLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKTLFFQKALLNNQSIRAILVP
NINLNKKQVQTLEKFAKDKGAKGLS*ISIKDEKIIDGSLLSIQEDHIIYKTIFKDFNLSTGSILLVADTFDIASQALGLV
RINLASILNLKKPNIFKFV*IID*PLYEYDNEAQRFVAAHHPFTMPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSV
RIIDQQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVVAFPKNNNGVDMMLDAP
SNMNDEDLKELGLKIKND
>Mature_578_residues
MKVYCGYIGEEHLEKNVILNG*VKKVRKMGNLVFVDLKDRFGIVQIFATKSDGVFNELTQLSREDVINVEGLVLLRKNPN
NDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFRYRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETET
PILAKPTPEGARDFYVPTRTRKFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEI
QTLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKTLFFQKALLNNQSIRAILVP
NINLNKKQVQTLEKFAKDKGAKGLS*ISIKDEKIIDGSLLSIQEDHIIYKTIFKDFNLSTGSILLVADTFDIASQALGLV
RINLASILNLKKPNIFKFV*IID*PLYEYDNEAQRFVAAHHPFTMPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSV
RIIDQQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVVAFPKNNNGVDMMLDAP
SNMNDEDLKELGLKIKND

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=586, Percent_Identity=36.518771331058, Blast_Score=350, Evalue=3e-96,
Organism=Homo sapiens, GI45439306, Length=287, Percent_Identity=26.1324041811847, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI5031815, Length=275, Percent_Identity=26.9090909090909, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI194272210, Length=275, Percent_Identity=26.9090909090909, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=585, Percent_Identity=38.974358974359, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1789256, Length=284, Percent_Identity=28.169014084507, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1790571, Length=285, Percent_Identity=27.0175438596491, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32566633, Length=584, Percent_Identity=34.9315068493151, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17551876, Length=265, Percent_Identity=26.7924528301887, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=196, Percent_Identity=28.0612244897959, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17535925, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17535927, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=624, Percent_Identity=33.1730769230769, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6323011, Length=257, Percent_Identity=29.9610894941634, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24584738, Length=592, Percent_Identity=35.304054054054, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17136276, Length=251, Percent_Identity=27.8884462151394, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640849, Length=283, Percent_Identity=26.1484098939929, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640851, Length=287, Percent_Identity=25.7839721254355, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66191; Mature: 66191

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYCGYIGEEHLEKNVILNGVKKVRKMGNLVFVDLKDRFGIVQIFATKSDGVFNELTQL
CEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
SREDVINVEGLVLLRKNPNNDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFR
HHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEEEEECCCCCCEEEECCCCCCCCEEEE
YRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETETPILAKPTPEGARDFYVPTRTR
EEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
KFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEIQ
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEEEEECCCCHHH
TLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKT
HHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEHHHCCCCE
LFFQKALLNNQSIRAILVPNINLNKKQVQTLEKFAKDKGAKGLSISIKDEKIIDGSLLSI
EEEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCEEEE
QEDHIIYKTIFKDFNLSTGSILLVADTFDIASQALGLVRINLASILNLKKPNIFKFVIID
ECCCEEEEHHHHHCCCCCCCEEEEEECHHHHHHHHHHEEEEHHHHHCCCCCCEEEEEEEC
PLYEYDNEAQRFVAAHHPFTMPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSVRIID
CHHHCCCHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEEH
QQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVV
HHHHHHHHHHCCCCCCCCCEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
AFPKNNNGVDMMLDAPSNMNDEDLKELGLKIKND
HCCCCCCCEEEEEECCCCCCHHHHHHHCCEECCC
>Mature Secondary Structure
MKVYCGYIGEEHLEKNVILNGVKKVRKMGNLVFVDLKDRFGIVQIFATKSDGVFNELTQL
CEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
SREDVINVEGLVLLRKNPNNDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFR
HHHCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEEEEECCCCCCEEEECCCCCCCCEEEE
YRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETETPILAKPTPEGARDFYVPTRTR
EEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
KFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEIQ
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEEEEECCCCHHH
TLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKT
HHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEHHHCCCCE
LFFQKALLNNQSIRAILVPNINLNKKQVQTLEKFAKDKGAKGLSISIKDEKIIDGSLLSI
EEEEHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCEEEE
QEDHIIYKTIFKDFNLSTGSILLVADTFDIASQALGLVRINLASILNLKKPNIFKFVIID
ECCCEEEEHHHHHCCCCCCCEEEEEECHHHHHHHHHHEEEEHHHHHCCCCCCEEEEEEEC
PLYEYDNEAQRFVAAHHPFTMPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSVRIID
CHHHCCCHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCEEEEEH
QQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVV
HHHHHHHHHHCCCCCCCCCEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
AFPKNNNGVDMMLDAPSNMNDEDLKELGLKIKND
HCCCCCCCEEEEEECCCCCCHHHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]