Click here to switch to the map view.

The map label for this gene is hlyU

Identifier: 15004735

GI number: 15004735

Start: 31999

End: 32286

Strand: Direct

Name: hlyU

Synonym: CA_P0031

Alternate gene names: 15004735

Gene position: 31999-32286 (Clockwise)

Preceding gene: 15004734

Following gene: 15004736

Centisome position: 16.67

GC content: 29.86

Gene sequence:

>288_bases
ATGATTGATAATGCTAGGGAAATAGCAGAGTTACTAAAAGTTCTAGCTAATGAAAATAGATTAATGATTGTTTGTCATCT
TATTGATGCACCTATGACAGTAACTGAACTTCATGAAAAAATAAATACCTTGACACAATCAGCACTGTCTCAGCATTTAG
CTTTATTGAGAGCACATGGAATACTTAGCTCAGAGAAAAGGGGACTTTCTATTACCTATTCAATAAAGGATAATAGGATA
ACAAATGTAATTAAGGTTTTAAAAGAAAATTATTGTGATATAAAGTAA

Upstream 100 bases:

>100_bases
AAACAAAATATGATTTTTAATTATAGGTTGACAACCCCACAGTTCTTGGGATAACATTATTTTATAATATACTTATATAA
TGTTCCTAGGAGGAGTTTTA

Downstream 100 bases:

>100_bases
AGCATGCAAAACTTATAAAGTAATTGCATGCTTTTGCATTTTATATTGACAAAAGTATATTCTAGATTTATATTAGTATA
TAAGAAGATACTAATATAAA

Product: transcriptional activator HlyU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MIDNAREIAELLKVLANENRLMIVCHLIDAPMTVTELHEKINTLTQSALSQHLALLRAHGILSSEKRGLSITYSIKDNRI
TNVIKVLKENYCDIK

Sequences:

>Translated_95_residues
MIDNAREIAELLKVLANENRLMIVCHLIDAPMTVTELHEKINTLTQSALSQHLALLRAHGILSSEKRGLSITYSIKDNRI
TNVIKVLKENYCDIK
>Mature_95_residues
MIDNAREIAELLKVLANENRLMIVCHLIDAPMTVTELHEKINTLTQSALSQHLALLRAHGILSSEKRGLSITYSIKDNRI
TNVIKVLKENYCDIK

Specific function: Up-regulates the expression of the hemolysin gene, hlyA, and may promote expression of other virulence determinants in vivo. It may have both positive and negative regulator activities [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=89, Percent_Identity=38.2022471910112, Blast_Score=66, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 10753; Mature: 10753

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNAREIAELLKVLANENRLMIVCHLIDAPMTVTELHEKINTLTQSALSQHLALLRAHG
CCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ILSSEKRGLSITYSIKDNRITNVIKVLKENYCDIK
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIDNAREIAELLKVLANENRLMIVCHLIDAPMTVTELHEKINTLTQSALSQHLALLRAHG
CCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ILSSEKRGLSITYSIKDNRITNVIKVLKENYCDIK
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8231807; 9457888; 10952301 [H]