Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 161511079

GI number: 161511079

Start: 465518

End: 467278

Strand: Reverse

Name: aspS [H]

Synonym: BB0446

Alternate gene names: 161511079

Gene position: 467278-465518 (Counterclockwise)

Preceding gene: 15594792

Following gene: 15594781

Centisome position: 51.31

GC content: 28.51

Gene sequence:

>1761_bases
ATGTTTAAAGTTATCAAATGTAATGAATTGAATGAAAAATTGATAGACAAAAAAGTTGAAATAAATGCTTGGGTAAAAAA
AATTAGACACCACGGAAAATTTATCTTTCTAAATATAAGAGACAGATATGAAAAAGCTCAAGTTCTGGTAAATGAAGAAA
AGCTTCTAAAGATCGCAGAAAAAATAAAACTTGAATATTGCATTAAAATTCAAGGACTGTTGATCAAAAGACCCCCCAAC
ATGATAAATGCAAATATGAAAACAGGACATTTTGAAATATTGGCAAAAAACATTGAAATTATCTCAAAGTGCAATGAATT
GCCATTTATGATAGAAGATGACAATAATGCAAGTGAAAACTCAAAACTTGAATACAGATACTTAGATTTAAGAAGAGATT
CCTTGAAAAATAAAATTATTTTAAGATGTCAGGCTACTCATCTTATTAGAAATTTTTTAATAAAAAGAAAATTTTTAGAG
CTAGAAACTCCAACTTTTGTAAAATCAACGCCAGAAGGTGCAAGAGATTTTGTAATCCCATCAAGGATTCACAAAGGATC
TTTTTATGCACTACCTCAATCTCCACAACTTTACAAACAACTCATAATGATAGCAGGATTTGACAAATACTTTCAAATAG
CCCGCTGCTATAGAGACGAAGATTCAAGAGGGGACAGACAACCAGAGTTCACCCAGCTCGATCTTGAAATGAGCTTTGTC
AAAAAAGAAAATATTTTTAAATTAATAGAAAATATGCTTTTTTTAATATTCAAAAATTGCATCAATATTAACCTACCTAA
AAAATTCAAAAAAATAACATACAAAAAGGCAATGAACAAATATGGAAGCGACAAACCAGATACTAGATTTGAACTTGAAT
TACAAGATATAAGTCGTAATCTAAAAAATTCAGAATTTAATATATTCAAAGATACTCTAAAAAACAAAGGTTCAATTAAA
ATTTTAATAGTAAAAGATAAAGCTGACAAGTTTTCAAGAGCAAAAATAAACAATTTAGAAGAAATTGCAAAGCTTTACAA
AACACAAGGGCTTTATTTTACAAAAATTGAAAACAATAAATTTTCCGGGGGAATTGCAAAATTTTTAAAAACAGAAGAAC
AGGAATTAATAAAAACCTATTCTTTAGAAAATAATGACATAATTTTCTTTACAGCTAATAATAACTGGGAAACTGCATGT
AAAGCAATGGGTCAAATTAGAATAAAAATTGCAAATGATCTTGGACTAATAGATGAAAATAAATTTGAATTTCTATGGGT
CTATGATTTTCCACTATTTGAATATGACGAAAATACAAAAACCTATTCACCAGCTCACCACATGTTCTCGCTTCCCAAAA
AGCAATATATTGCTAATTTAGAAAAAAATCCAAAAAAAACTATAGGTGAAATTTACGATCTTGTTTTAAATGGCGTAGAA
CTTGGCTCAGGTTCAATTAGAATACATAACAAAGAGCTTCAACAAAGAATTTTCAAAATAATAGGATTTCAAAAAGAAAA
ATCAGAAGATAGATTTGGATTTTTTCTAAAAGCATTAGAATATGGAGCTCCTAATCATGGTGGCATTGCTATTGGCATTG
ATAGACTAATAATGTTGATGACAAAATCAACTTCAATAAAAGATGTAATACTGTTTCCTAAAAATTCTTTTGCAGCAAGC
CCTCTTGATAATTCCCCCTCTAAAATCTCAAATGAACAACTCAAAGAACTGGGAATTAATATTGTTGATGGTGACAATTA
A

Upstream 100 bases:

>100_bases
AATTTTAGCATCTGTTGTGGGCAAAGTTGATCTGTACAAAAAAGAAATTTTAAATGCAAAAACAGATAAAGAAATTTATA
ATATAATAGTGAGCTAAGTT

Downstream 100 bases:

>100_bases
TCCATAATGCAAGCTCTGCCATATACCTTTAAAATTATAAAAATCAGCTACTCAACATTCCTTGGAATTTTTCAAGATAC
TGCTATTCTTTCAAGAAATT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MFKVIKCNELNEKLIDKKVEINAWVKKIRHHGKFIFLNIRDRYEKAQVLVNEEKLLKIAEKIKLEYCIKIQGLLIKRPPN
MINANMKTGHFEILAKNIEIISKCNELPFMIEDDNNASENSKLEYRYLDLRRDSLKNKIILRCQATHLIRNFLIKRKFLE
LETPTFVKSTPEGARDFVIPSRIHKGSFYALPQSPQLYKQLIMIAGFDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFV
KKENIFKLIENMLFLIFKNCININLPKKFKKITYKKAMNKYGSDKPDTRFELELQDISRNLKNSEFNIFKDTLKNKGSIK
ILIVKDKADKFSRAKINNLEEIAKLYKTQGLYFTKIENNKFSGGIAKFLKTEEQELIKTYSLENNDIIFFTANNNWETAC
KAMGQIRIKIANDLGLIDENKFEFLWVYDFPLFEYDENTKTYSPAHHMFSLPKKQYIANLEKNPKKTIGEIYDLVLNGVE
LGSGSIRIHNKELQQRIFKIIGFQKEKSEDRFGFFLKALEYGAPNHGGIAIGIDRLIMLMTKSTSIKDVILFPKNSFAAS
PLDNSPSKISNEQLKELGINIVDGDN

Sequences:

>Translated_586_residues
MFKVIKCNELNEKLIDKKVEINAWVKKIRHHGKFIFLNIRDRYEKAQVLVNEEKLLKIAEKIKLEYCIKIQGLLIKRPPN
MINANMKTGHFEILAKNIEIISKCNELPFMIEDDNNASENSKLEYRYLDLRRDSLKNKIILRCQATHLIRNFLIKRKFLE
LETPTFVKSTPEGARDFVIPSRIHKGSFYALPQSPQLYKQLIMIAGFDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFV
KKENIFKLIENMLFLIFKNCININLPKKFKKITYKKAMNKYGSDKPDTRFELELQDISRNLKNSEFNIFKDTLKNKGSIK
ILIVKDKADKFSRAKINNLEEIAKLYKTQGLYFTKIENNKFSGGIAKFLKTEEQELIKTYSLENNDIIFFTANNNWETAC
KAMGQIRIKIANDLGLIDENKFEFLWVYDFPLFEYDENTKTYSPAHHMFSLPKKQYIANLEKNPKKTIGEIYDLVLNGVE
LGSGSIRIHNKELQQRIFKIIGFQKEKSEDRFGFFLKALEYGAPNHGGIAIGIDRLIMLMTKSTSIKDVILFPKNSFAAS
PLDNSPSKISNEQLKELGINIVDGDN
>Mature_586_residues
MFKVIKCNELNEKLIDKKVEINAWVKKIRHHGKFIFLNIRDRYEKAQVLVNEEKLLKIAEKIKLEYCIKIQGLLIKRPPN
MINANMKTGHFEILAKNIEIISKCNELPFMIEDDNNASENSKLEYRYLDLRRDSLKNKIILRCQATHLIRNFLIKRKFLE
LETPTFVKSTPEGARDFVIPSRIHKGSFYALPQSPQLYKQLIMIAGFDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFV
KKENIFKLIENMLFLIFKNCININLPKKFKKITYKKAMNKYGSDKPDTRFELELQDISRNLKNSEFNIFKDTLKNKGSIK
ILIVKDKADKFSRAKINNLEEIAKLYKTQGLYFTKIENNKFSGGIAKFLKTEEQELIKTYSLENNDIIFFTANNNWETAC
KAMGQIRIKIANDLGLIDENKFEFLWVYDFPLFEYDENTKTYSPAHHMFSLPKKQYIANLEKNPKKTIGEIYDLVLNGVE
LGSGSIRIHNKELQQRIFKIIGFQKEKSEDRFGFFLKALEYGAPNHGGIAIGIDRLIMLMTKSTSIKDVILFPKNSFAAS
PLDNSPSKISNEQLKELGINIVDGDN

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=590, Percent_Identity=38.6440677966102, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI45439306, Length=331, Percent_Identity=25.0755287009063, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI5031815, Length=241, Percent_Identity=27.8008298755187, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI194272210, Length=241, Percent_Identity=27.8008298755187, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI226491502, Length=268, Percent_Identity=22.7611940298507, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=40.3747870528109, Blast_Score=480, Evalue=1e-136,
Organism=Escherichia coli, GI1789256, Length=301, Percent_Identity=25.9136212624585, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790571, Length=292, Percent_Identity=25, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=34.5575959933222, Blast_Score=341, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI17551876, Length=283, Percent_Identity=30.3886925795053, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71984122, Length=299, Percent_Identity=27.4247491638796, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=635, Percent_Identity=32.9133858267717, Blast_Score=266, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6323011, Length=293, Percent_Identity=30.3754266211604, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320242, Length=211, Percent_Identity=26.5402843601896, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24584738, Length=592, Percent_Identity=38.1756756756757, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136276, Length=278, Percent_Identity=28.4172661870504, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=25.0980392156863, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=25.0980392156863, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 68344; Mature: 68344

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVIKCNELNEKLIDKKVEINAWVKKIRHHGKFIFLNIRDRYEKAQVLVNEEKLLKIAE
CEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHEECHHHHHHHHH
KIKLEYCIKIQGLLIKRPPNMINANMKTGHFEILAKNIEIISKCNELPFMIEDDNNASEN
HHHHEEEEEEEEEEEECCCCCEECCCCCCCCEEHHHHHHHHHHCCCCCEEEECCCCCCCC
SKLEYRYLDLRRDSLKNKIILRCQATHLIRNFLIKRKFLELETPTFVKSTPEGARDFVIP
CEEEEEEEHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SRIHKGSFYALPQSPQLYKQLIMIAGFDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFV
CCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHH
KKENIFKLIENMLFLIFKNCININLPKKFKKITYKKAMNKYGSDKPDTRFELELQDISRN
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHH
LKNSEFNIFKDTLKNKGSIKILIVKDKADKFSRAKINNLEEIAKLYKTQGLYFTKIENNK
CCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHCCEEEEEECCCC
FSGGIAKFLKTEEQELIKTYSLENNDIIFFTANNNWETACKAMGQIRIKIANDLGLIDEN
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCEEEEEEECCCCCCCCC
KFEFLWVYDFPLFEYDENTKTYSPAHHMFSLPKKQYIANLEKNPKKTIGEIYDLVLNGVE
CEEEEEEEECCEEEECCCCCCCCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCEE
LGSGSIRIHNKELQQRIFKIIGFQKEKSEDRFGFFLKALEYGAPNHGGIAIGIDRLIMLM
ECCCEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHH
TKSTSIKDVILFPKNSFAASPLDNSPSKISNEQLKELGINIVDGDN
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCC
>Mature Secondary Structure
MFKVIKCNELNEKLIDKKVEINAWVKKIRHHGKFIFLNIRDRYEKAQVLVNEEKLLKIAE
CEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHEECHHHHHHHHH
KIKLEYCIKIQGLLIKRPPNMINANMKTGHFEILAKNIEIISKCNELPFMIEDDNNASEN
HHHHEEEEEEEEEEEECCCCCEECCCCCCCCEEHHHHHHHHHHCCCCCEEEECCCCCCCC
SKLEYRYLDLRRDSLKNKIILRCQATHLIRNFLIKRKFLELETPTFVKSTPEGARDFVIP
CEEEEEEEHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SRIHKGSFYALPQSPQLYKQLIMIAGFDKYFQIARCYRDEDSRGDRQPEFTQLDLEMSFV
CCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHH
KKENIFKLIENMLFLIFKNCININLPKKFKKITYKKAMNKYGSDKPDTRFELELQDISRN
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHH
LKNSEFNIFKDTLKNKGSIKILIVKDKADKFSRAKINNLEEIAKLYKTQGLYFTKIENNK
CCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHCCEEEEEECCCC
FSGGIAKFLKTEEQELIKTYSLENNDIIFFTANNNWETACKAMGQIRIKIANDLGLIDEN
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCEEEEEEECCCCCCCCC
KFEFLWVYDFPLFEYDENTKTYSPAHHMFSLPKKQYIANLEKNPKKTIGEIYDLVLNGVE
CEEEEEEEECCEEEECCCCCCCCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHCCEE
LGSGSIRIHNKELQQRIFKIIGFQKEKSEDRFGFFLKALEYGAPNHGGIAIGIDRLIMLM
ECCCEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHH
TKSTSIKDVILFPKNSFAASPLDNSPSKISNEQLKELGINIVDGDN
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA