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The map label for this gene is sigA [H]

Identifier: 15595116

GI number: 15595116

Start: 812439

End: 813239

Strand: Reverse

Name: sigA [H]

Synonym: BB0771

Alternate gene names: 15595116

Gene position: 813239-812439 (Counterclockwise)

Preceding gene: 15595117

Following gene: 15595115

Centisome position: 89.3

GC content: 27.97

Gene sequence:

>801_bases
ATGAACATATTTAGTAATGAGGATTTAAACATATATTTAAAATCAGTAAGAGAACACAAGCTAATTACTCACGAAGAAGA
AATCAAACTTGCAGGACAAATACAAAGAGGCAATGCAAAAGCAAAAAACAAGATGATAAATGCAAACTTGCGACTTGTTT
TAAAAATAATAAAAAGATATGCGGGTAAAGGGTTAAAAATTGAAGACTTAATTCAAGAAGGCAACTTGGGATTAATAAGA
GCTGCTGAAAAATATGACCCGAATAAAAATACCAAATTTTCAACTTATGCATCATTTTGGATTAAGCAATCACTACAAAG
AGCATTAAACACTAAAACCAGATTGGTAAAAGTCCCATACAGAAAAGAAAATCTAATACTACAAATAAATAAATATTTAA
CAGAAGAAGAAAAATCGCCCAAAAAAGAAGAAATAATGAAAAGATTCAACCTATCTCCTGCTCAGTATATAAAAATTATT
CCCTATCTTGAAAAAGAATATTCTCTGGACAAAGAAATAGAGGGATCTGAAAATTCAACACTCTTGAATCTATACGAGGA
TAATTCTTTTAACCCTGAAATTACCCTTGAACAAGATTCAACTCTAAAACATTTGAATTATATACTTGAAACAAAATTAA
ATGAAAAGGAAAGATACATAATTAAAAAAAGATATAACCTGGACAATAGTCCCAAAAAAAGCACCTTAAAAGATATTTCA
ACAGAACTTGGAATATCATCAGAAACTGTAAGACAGATTGAAAAAAGAGTTCTTAAAAAATTAAAAGAAGAAATAAATTA
A

Upstream 100 bases:

>100_bases
TAAAACCATTTTTAAATTAAATTGGCACAGTTTTTGCATGGAAATTAAGTAGTAAAAACTTAATCACAATATTCAAGAAA
GGGGAGAAAATATAATAACT

Downstream 100 bases:

>100_bases
CATTGACATTCATGACATGTTCTGGTCTACTTGTAAGTCAGTGGTCATGAATGTTTGTATTTTATATTAAAAAAAACAAG
TTTATTATTGTAATTTTTAT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MNIFSNEDLNIYLKSVREHKLITHEEEIKLAGQIQRGNAKAKNKMINANLRLVLKIIKRYAGKGLKIEDLIQEGNLGLIR
AAEKYDPNKNTKFSTYASFWIKQSLQRALNTKTRLVKVPYRKENLILQINKYLTEEEKSPKKEEIMKRFNLSPAQYIKII
PYLEKEYSLDKEIEGSENSTLLNLYEDNSFNPEITLEQDSTLKHLNYILETKLNEKERYIIKKRYNLDNSPKKSTLKDIS
TELGISSETVRQIEKRVLKKLKEEIN

Sequences:

>Translated_266_residues
MNIFSNEDLNIYLKSVREHKLITHEEEIKLAGQIQRGNAKAKNKMINANLRLVLKIIKRYAGKGLKIEDLIQEGNLGLIR
AAEKYDPNKNTKFSTYASFWIKQSLQRALNTKTRLVKVPYRKENLILQINKYLTEEEKSPKKEEIMKRFNLSPAQYIKII
PYLEKEYSLDKEIEGSENSTLLNLYEDNSFNPEITLEQDSTLKHLNYILETKLNEKERYIIKKRYNLDNSPKKSTLKDIS
TELGISSETVRQIEKRVLKKLKEEIN
>Mature_266_residues
MNIFSNEDLNIYLKSVREHKLITHEEEIKLAGQIQRGNAKAKNKMINANLRLVLKIIKRYAGKGLKIEDLIQEGNLGLIR
AAEKYDPNKNTKFSTYASFWIKQSLQRALNTKTRLVKVPYRKENLILQINKYLTEEEKSPKKEEIMKRFNLSPAQYIKII
PYLEKEYSLDKEIEGSENSTLLNLYEDNSFNPEITLEQDSTLKHLNYILETKLNEKERYIIKKRYNLDNSPKKSTLKDIS
TELGISSETVRQIEKRVLKKLKEEIN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=263, Percent_Identity=36.5019011406844, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789448, Length=257, Percent_Identity=29.1828793774319, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=27.112676056338, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31238; Mature: 31238

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFSNEDLNIYLKSVREHKLITHEEEIKLAGQIQRGNAKAKNKMINANLRLVLKIIKRY
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCHHHHHHHCCHHHHHHHHHHHH
AGKGLKIEDLIQEGNLGLIRAAEKYDPNKNTKFSTYASFWIKQSLQRALNTKTRLVKVPY
CCCCCCHHHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
RKENLILQINKYLTEEEKSPKKEEIMKRFNLSPAQYIKIIPYLEKEYSLDKEIEGSENST
CCCCEEEEEHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
LLNLYEDNSFNPEITLEQDSTLKHLNYILETKLNEKERYIIKKRYNLDNSPKKSTLKDIS
EEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
TELGISSETVRQIEKRVLKKLKEEIN
HHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIFSNEDLNIYLKSVREHKLITHEEEIKLAGQIQRGNAKAKNKMINANLRLVLKIIKRY
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCHHHHHHHCCHHHHHHHHHHHH
AGKGLKIEDLIQEGNLGLIRAAEKYDPNKNTKFSTYASFWIKQSLQRALNTKTRLVKVPY
CCCCCCHHHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
RKENLILQINKYLTEEEKSPKKEEIMKRFNLSPAQYIKIIPYLEKEYSLDKEIEGSENST
CCCCEEEEEHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
LLNLYEDNSFNPEITLEQDSTLKHLNYILETKLNEKERYIIKKRYNLDNSPKKSTLKDIS
EEEEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
TELGISSETVRQIEKRVLKKLKEEIN
HHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]