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The map label for this gene is dnaG

Identifier: 15595055

GI number: 15595055

Start: 746339

End: 747910

Strand: Direct

Name: dnaG

Synonym: BB0710

Alternate gene names: 15595055

Gene position: 746339-747910 (Clockwise)

Preceding gene: 15595054

Following gene: 15595056

Centisome position: 81.95

GC content: 27.23

Gene sequence:

>1572_bases
ATGAAGTATTTACAAACTGTAGCTTCAATGAAAAGCAAATTTGATATTGTAGCTATTGTGGAGCAATATATTAAGCTTGT
TAAATCGGGATCTGCTTACAAAGGTCTTTGTCCTTTTCATGCTGAGAAGACTCCCTCTTTTTTTGTAAATCCTTTGCAAG
GATATTTTTATTGTTTTGGATGCAAAAAGGGTGGAGATGTTATTGGATTTTTAATGGATATGGAAAAAATCAATTACAAT
GATGCTCTTAAGATTTTATGCGAAAAATCCGGTATTCATTATGATGATTTAAAAATAAGTCGAGGAAGTGAAAATAAAAA
TGAAAATAAAGACATGGTTTCAAAAATTTACTCTTTGAATTCTCGGTTAATTAATACCATTAAATTTTTTTTAAGTAAAA
ACAAAAAAGCTTTAGATTATGTTTTAAAGAGTAGAGCAATATCTAAGGAAATTGTTGATTTATTTGAACTTGGTTATTTG
CCATTTAACTTTAAAAATGGTTTAGAGCTTCATGATTTTTTAGTTTCAAAAGGATACTCTTCTGAAGTACTTAGAAAAAG
TGGTTTGTTCTCGAAAACCAATCCCAAAGTTTCTATTTTATTTCAAAGATTAATTTTTCCAATTAAAGACTTTAAAGGAA
ACGTTGTTGGTTTTGGAGGTCGAGATTTAGATGGGAAAGGTTCTAAGTATATTAATTTAGGTGAAACTGAAGTTTTTAAA
AAAAGGGAGCTTCTTTATGGATTTTATGAGGGTTTTGAGGAGATTAAATCTACAAAATCAGTTATATTGGTAGAAGGGTA
TATAGATGTTCTTGCTTTTTTTACATCTGGGATTAAGAGAGCCGTATCTACTCTTGGCACTGCTTTTTCAAAAGAGCATT
TAGCTTTAATTCAAAGATATGCTGATGAGATAATATTTTCTTTTGATGGGGATGATGCTGGACTTTCTGCAACTTTAAAA
GCTTATCAAATTTGTTTGCCGTTTAATATCAATGTTAGTGTTGTTCGAATGGATTTTGGTACTGATCCTGCAGATGTTCT
TAAAAGTGAGGGTGTAGACTCCTTGCAAAAAATTTTAAATAATAGATGTGATGCTTTTGAATATCTTTTGGATGTTTATT
CTAATAAATATAATTTAAATAAAACTGTAGATTTAAATGCCATGATTAATTTATTTTTAAATTTGATAAATTTATCAAAA
GTAGATACTCAGAAAAAAATTTTTTTAGACAAGCTAAGCAATAAACTTGGTATTGGTGTGACAACTTTATTGAAAGATTA
TTACAGAATAAAAGAAAGATTTGTAGTTGACAATAATAAAAGAAATTTGTATGCTCATAATGATGATTCTTATGAGAGGT
ATCTAATAGTAGCTTTGTTGAAAAATTTTAGTTATTTTAGCATAGTAAGGCGCAATATTATTGATAGTGATTTAATTAAT
GTCGATGCCAGAAAAGTTTTTATGTGCTTTGAAAATTTATTTGAAAATAATAAAGATTTTTCATTAATGGATTTAAAAAA
AAATTTAAAGGATACCTATAAAGTTAGTGAATTTTTTTTGAAGAAATTTTAA

Upstream 100 bases:

>100_bases
ACTCTAAGTTGGGCACACATCAATTTTCATCAGAATATTCTTCACATCTTTTAGGGGCAAAAGATTATATTAAAAATTTT
ATTACTAAGGATTAAGAAGT

Downstream 100 bases:

>100_bases
ATTCTGAATTTGAAGTGGATGATGAGATGCTCATTCATATTTTACTTGCAATCAAGAGAAGAAAATTAGATTCTCGTGTT
TTGCTTTGCAAAAAAAGATA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MKYLQTVASMKSKFDIVAIVEQYIKLVKSGSAYKGLCPFHAEKTPSFFVNPLQGYFYCFGCKKGGDVIGFLMDMEKINYN
DALKILCEKSGIHYDDLKISRGSENKNENKDMVSKIYSLNSRLINTIKFFLSKNKKALDYVLKSRAISKEIVDLFELGYL
PFNFKNGLELHDFLVSKGYSSEVLRKSGLFSKTNPKVSILFQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLGETEVFK
KRELLYGFYEGFEEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTAFSKEHLALIQRYADEIIFSFDGDDAGLSATLK
AYQICLPFNINVSVVRMDFGTDPADVLKSEGVDSLQKILNNRCDAFEYLLDVYSNKYNLNKTVDLNAMINLFLNLINLSK
VDTQKKIFLDKLSNKLGIGVTTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFSIVRRNIIDSDLIN
VDARKVFMCFENLFENNKDFSLMDLKKNLKDTYKVSEFFLKKF

Sequences:

>Translated_523_residues
MKYLQTVASMKSKFDIVAIVEQYIKLVKSGSAYKGLCPFHAEKTPSFFVNPLQGYFYCFGCKKGGDVIGFLMDMEKINYN
DALKILCEKSGIHYDDLKISRGSENKNENKDMVSKIYSLNSRLINTIKFFLSKNKKALDYVLKSRAISKEIVDLFELGYL
PFNFKNGLELHDFLVSKGYSSEVLRKSGLFSKTNPKVSILFQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLGETEVFK
KRELLYGFYEGFEEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTAFSKEHLALIQRYADEIIFSFDGDDAGLSATLK
AYQICLPFNINVSVVRMDFGTDPADVLKSEGVDSLQKILNNRCDAFEYLLDVYSNKYNLNKTVDLNAMINLFLNLINLSK
VDTQKKIFLDKLSNKLGIGVTTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFSIVRRNIIDSDLIN
VDARKVFMCFENLFENNKDFSLMDLKKNLKDTYKVSEFFLKKF
>Mature_523_residues
MKYLQTVASMKSKFDIVAIVEQYIKLVKSGSAYKGLCPFHAEKTPSFFVNPLQGYFYCFGCKKGGDVIGFLMDMEKINYN
DALKILCEKSGIHYDDLKISRGSENKNENKDMVSKIYSLNSRLINTIKFFLSKNKKALDYVLKSRAISKEIVDLFELGYL
PFNFKNGLELHDFLVSKGYSSEVLRKSGLFSKTNPKVSILFQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLGETEVFK
KRELLYGFYEGFEEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTAFSKEHLALIQRYADEIIFSFDGDDAGLSATLK
AYQICLPFNINVSVVRMDFGTDPADVLKSEGVDSLQKILNNRCDAFEYLLDVYSNKYNLNKTVDLNAMINLFLNLINLSK
VDTQKKIFLDKLSNKLGIGVTTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFSIVRRNIIDSDLIN
VDARKVFMCFENLFENNKDFSLMDLKKNLKDTYKVSEFFLKKF

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=414, Percent_Identity=29.7101449275362, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_BORBU (O51653)

Other databases:

- EMBL:   AE000783
- PIR:   E70188
- RefSeq:   NP_212844.1
- ProteinModelPortal:   O51653
- SMR:   O51653
- EnsemblBacteria:   EBBORT00000009053
- GeneID:   1195564
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0710
- NMPDR:   fig|224326.1.peg.1094
- TIGR:   BB_0710
- GeneTree:   EBGT00050000007335
- HOGENOM:   HBG354484
- OMA:   ICLPFNI
- ProtClustDB:   CLSK507779
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 59981; Mature: 59981

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLQTVASMKSKFDIVAIVEQYIKLVKSGSAYKGLCPFHAEKTPSFFVNPLQGYFYCFG
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEE
CKKGGDVIGFLMDMEKINYNDALKILCEKSGIHYDDLKISRGSENKNENKDMVSKIYSLN
CCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEECCEEEECCCCCCCCCHHHHHHHHHHH
SRLINTIKFFLSKNKKALDYVLKSRAISKEIVDLFELGYLPFNFKNGLELHDFLVSKGYS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCH
SEVLRKSGLFSKTNPKVSILFQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLGETEVFK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEECCHHHHHH
KRELLYGFYEGFEEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTAFSKEHLALIQRY
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADEIIFSFDGDDAGLSATLKAYQICLPFNINVSVVRMDFGTDPADVLKSEGVDSLQKILN
HHHEEEEECCCCCCHHHEEEHEEEEEEECCCEEEEEEECCCCHHHHHHHCCHHHHHHHHH
NRCDAFEYLLDVYSNKYNLNKTVDLNAMINLFLNLINLSKVDTQKKIFLDKLSNKLGIGV
CCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHH
TTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFSIVRRNIIDSDLIN
HHHHHHHHHHHHHHEEECCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHHC
VDARKVFMCFENLFENNKDFSLMDLKKNLKDTYKVSEFFLKKF
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYLQTVASMKSKFDIVAIVEQYIKLVKSGSAYKGLCPFHAEKTPSFFVNPLQGYFYCFG
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEE
CKKGGDVIGFLMDMEKINYNDALKILCEKSGIHYDDLKISRGSENKNENKDMVSKIYSLN
CCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEECCEEEECCCCCCCCCHHHHHHHHHHH
SRLINTIKFFLSKNKKALDYVLKSRAISKEIVDLFELGYLPFNFKNGLELHDFLVSKGYS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCH
SEVLRKSGLFSKTNPKVSILFQRLIFPIKDFKGNVVGFGGRDLDGKGSKYINLGETEVFK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEECCHHHHHH
KRELLYGFYEGFEEIKSTKSVILVEGYIDVLAFFTSGIKRAVSTLGTAFSKEHLALIQRY
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADEIIFSFDGDDAGLSATLKAYQICLPFNINVSVVRMDFGTDPADVLKSEGVDSLQKILN
HHHEEEEECCCCCCHHHEEEHEEEEEEECCCEEEEEEECCCCHHHHHHHCCHHHHHHHHH
NRCDAFEYLLDVYSNKYNLNKTVDLNAMINLFLNLINLSKVDTQKKIFLDKLSNKLGIGV
CCCHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHH
TTLLKDYYRIKERFVVDNNKRNLYAHNDDSYERYLIVALLKNFSYFSIVRRNIIDSDLIN
HHHHHHHHHHHHHHEEECCCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHHC
VDARKVFMCFENLFENNKDFSLMDLKKNLKDTYKVSEFFLKKF
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685