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The map label for this gene is yccK [H]

Identifier: 15594873

GI number: 15594873

Start: 538328

End: 539275

Strand: Reverse

Name: yccK [H]

Synonym: BB0528

Alternate gene names: 15594873

Gene position: 539275-538328 (Counterclockwise)

Preceding gene: 15594875

Following gene: 15594870

Centisome position: 59.21

GC content: 26.48

Gene sequence:

>948_bases
GTGAATAATCTTAAAGACAAGATAAATACTTATAGCAAATTAATTTTAGGGTCTTGGCAATTCGGAGGAGGATATTTTAA
GCAAGTCGAAAAAGAAACTGCTAAAAAAATATTAAAAAAAGCATATGATCACGGGATCAGAAATATTGATACTGCAAGAG
CTTATGGAAATGGAATTTCGGAAAAAATAATTGGCGAAATAATAGAAAAAGATCCAACAATAAGAGAAAATATTTTAATT
GCAAGTAAATGCTACCCAATGGAAATTTCAGAATATAGAGAGAACTTTAATGAAAGTCTTAAAAATTTAAAAACTGATTA
TATAGATATTTACTATATACACTGGCCTAAAGCCGATTTTGACCTAAGACCAATCGCATCATTTCTTGAAGAAATGAGAG
TAAAAGGAAGAATAAAATATGTAGGCGTAAGTAATTTTGAAATATCACACATGGAAAGCATAAAAAAAGTTTGCAAAATT
GACGTAAACCAAATAGGATACAACCCCTTATTTAGAAATAAAGAAAAAGATGTAATTCCTTACTGTGAAGACAACAATAT
TGCCACCATATCATATTCAACAATTGCTCAAGGACTTTTATCTAAAGCTAATATAAAAGACAAAAACAAATTTAATGATA
TTAGAACAGAAAAATTGATACTTTTCAAAAAAGAAATTTGGCCTTATACTTTGAAAACCATAAATAAACTTGAAGAGATA
GCAAAGATAAATAACTTAACAATTTTAGAATTAACATATTCATGGCTTAAAAAAACAAAATTAAGTGGATTTATAGTGGG
CTTTAGCAAGGAAAATTATGTAGAATCAAACGTAAATTCATTTAAAGCAGAAATTAATGATAAAGTATATGAAGAGATTA
CATCAATTTTAGATAATTTCAATCACCAAACAAAAAACTTCCCAAATTTATTTAACAAAAAAATTTAA

Upstream 100 bases:

>100_bases
AAACTATAAAATTTTATTTATAAAACTTTTAAAATAAGACATACTTAAATCATCATTAAAACAAAAATACCAATTTATCG
TAAAATAAAAAGGAGAATCT

Downstream 100 bases:

>100_bases
AACTTTAAAAACAAATATTTCAATTAACAAACTTAAAGTCAATAGAATTTCCTAAAATTCTAACGCCTTCTACAGTTAAA
TGAATATTAAAAATAAACTC

Product: aldose reductase

Products: aldose; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNNLKDKINTYSKLILGSWQFGGGYFKQVEKETAKKILKKAYDHGIRNIDTARAYGNGISEKIIGEIIEKDPTIRENILI
ASKCYPMEISEYRENFNESLKNLKTDYIDIYYIHWPKADFDLRPIASFLEEMRVKGRIKYVGVSNFEISHMESIKKVCKI
DVNQIGYNPLFRNKEKDVIPYCEDNNIATISYSTIAQGLLSKANIKDKNKFNDIRTEKLILFKKEIWPYTLKTINKLEEI
AKINNLTILELTYSWLKKTKLSGFIVGFSKENYVESNVNSFKAEINDKVYEEITSILDNFNHQTKNFPNLFNKKI

Sequences:

>Translated_315_residues
MNNLKDKINTYSKLILGSWQFGGGYFKQVEKETAKKILKKAYDHGIRNIDTARAYGNGISEKIIGEIIEKDPTIRENILI
ASKCYPMEISEYRENFNESLKNLKTDYIDIYYIHWPKADFDLRPIASFLEEMRVKGRIKYVGVSNFEISHMESIKKVCKI
DVNQIGYNPLFRNKEKDVIPYCEDNNIATISYSTIAQGLLSKANIKDKNKFNDIRTEKLILFKKEIWPYTLKTINKLEEI
AKINNLTILELTYSWLKKTKLSGFIVGFSKENYVESNVNSFKAEINDKVYEEITSILDNFNHQTKNFPNLFNKKI
>Mature_315_residues
MNNLKDKINTYSKLILGSWQFGGGYFKQVEKETAKKILKKAYDHGIRNIDTARAYGNGISEKIIGEIIEKDPTIRENILI
ASKCYPMEISEYRENFNESLKNLKTDYIDIYYIHWPKADFDLRPIASFLEEMRVKGRIKYVGVSNFEISHMESIKKVCKI
DVNQIGYNPLFRNKEKDVIPYCEDNNIATISYSTIAQGLLSKANIKDKNKFNDIRTEKLILFKKEIWPYTLKTINKLEEI
AKINNLTILELTYSWLKKTKLSGFIVGFSKENYVESNVNSFKAEINDKVYEEITSILDNFNHQTKNFPNLFNKKI

Specific function: Unknown

COG id: COG0667

COG function: function code C; Predicted oxidoreductases (related to aryl-alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase 2 family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=235, Percent_Identity=31.063829787234, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4504825, Length=327, Percent_Identity=23.5474006116208, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27436969, Length=327, Percent_Identity=23.5474006116208, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27436964, Length=327, Percent_Identity=24.1590214067278, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI27436966, Length=327, Percent_Identity=24.1590214067278, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27436962, Length=327, Percent_Identity=24.1590214067278, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI291291012, Length=212, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310109922, Length=196, Percent_Identity=30.1020408163265, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI5453543, Length=215, Percent_Identity=28.8372093023256, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1788070, Length=307, Percent_Identity=28.9902280130293, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788081, Length=253, Percent_Identity=26.0869565217391, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081735, Length=291, Percent_Identity=26.1168384879725, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082198, Length=193, Percent_Identity=32.1243523316062, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789375, Length=292, Percent_Identity=25, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789199, Length=342, Percent_Identity=23.6842105263158, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786400, Length=186, Percent_Identity=31.1827956989247, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI48994888, Length=290, Percent_Identity=19.6551724137931, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17552492, Length=193, Percent_Identity=35.2331606217617, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17561300, Length=193, Percent_Identity=35.7512953367876, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17561298, Length=199, Percent_Identity=34.1708542713568, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538386, Length=310, Percent_Identity=25.4838709677419, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562292, Length=221, Percent_Identity=25.7918552036199, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71998625, Length=191, Percent_Identity=28.2722513089005, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17566692, Length=196, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325169, Length=319, Percent_Identity=25.705329153605, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323998, Length=323, Percent_Identity=24.1486068111455, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320079, Length=231, Percent_Identity=26.4069264069264, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319958, Length=302, Percent_Identity=23.841059602649, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319951, Length=229, Percent_Identity=26.6375545851528, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24657054, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646155, Length=301, Percent_Identity=23.2558139534884, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: 1.1.1.21

Molecular weight: Translated: 36755; Mature: 36755

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKDKINTYSKLILGSWQFGGGYFKQVEKETAKKILKKAYDHGIRNIDTARAYGNGIS
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHH
EKIIGEIIEKDPTIRENILIASKCYPMEISEYRENFNESLKNLKTDYIDIYYIHWPKADF
HHHHHHHHHCCCCHHHCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
DLRPIASFLEEMRVKGRIKYVGVSNFEISHMESIKKVCKIDVNQIGYNPLFRNKEKDVIP
CHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
YCEDNNIATISYSTIAQGLLSKANIKDKNKFNDIRTEKLILFKKEIWPYTLKTINKLEEI
EECCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHH
AKINNLTILELTYSWLKKTKLSGFIVGFSKENYVESNVNSFKAEINDKVYEEITSILDNF
HHHCCCEEEEHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NHQTKNFPNLFNKKI
CCCCCCCCHHHCCCC
>Mature Secondary Structure
MNNLKDKINTYSKLILGSWQFGGGYFKQVEKETAKKILKKAYDHGIRNIDTARAYGNGIS
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHH
EKIIGEIIEKDPTIRENILIASKCYPMEISEYRENFNESLKNLKTDYIDIYYIHWPKADF
HHHHHHHHHCCCCHHHCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
DLRPIASFLEEMRVKGRIKYVGVSNFEISHMESIKKVCKIDVNQIGYNPLFRNKEKDVIP
CHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
YCEDNNIATISYSTIAQGLLSKANIKDKNKFNDIRTEKLILFKKEIWPYTLKTINKLEEI
EECCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHH
AKINNLTILELTYSWLKKTKLSGFIVGFSKENYVESNVNSFKAEINDKVYEEITSILDNF
HHHCCCEEEEHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NHQTKNFPNLFNKKI
CCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: alditol; NAD+; NADP+

Specific reaction: alditol + NAD(P)+ = aldose + NAD(P)H + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 9106203 [H]