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The map label for this gene is rpoA

Identifier: 15594847

GI number: 15594847

Start: 507811

End: 508848

Strand: Direct

Name: rpoA

Synonym: BB0502

Alternate gene names: 15594847

Gene position: 507811-508848 (Clockwise)

Preceding gene: 15594846

Following gene: 15594848

Centisome position: 55.76

GC content: 30.92

Gene sequence:

>1038_bases
ATGATGCTTGTGGAAAAATTTTTGAAAGATTTCACTATACCTGAAAAAATTGAATTTTTGAAAAGCCAAGGTGATGGGTC
TTATGGTAAATTTACGATATATCCTTTTGAAAGAGGCTTTGGAATTACTATAGGTAATACTTTAAGACGTGTGTTACTTT
CTTCTATTGAAGGGTATGCGATTACTGCTATGAGAGTTCAGTCTAACAATAAAGACTCTTCGTCAAAGGTTGTTTCAAGT
GAATTTGATTTGATTCCTGGAGTTTCTGAAGATACTCTTGAGATTATTGCTAATATTAAAAATATTCATTTGAAACTTGG
AGAAGGAGAGCAAAGAAAGACAATAAGCTTTAGTGTTAGCGGTAAAGACACCAATGTTTTAAAAGCTTCTCATTTTGAAA
GAGATGGAGTTGAGGTTTTTAATAAAGATTTAGTTATAGCTACTTTATCACATGATGTTAATTTAGATCTTGAATTTCAA
ATTAATTATGGTAGGGGCTATGTGTCTTCTGAGCAAAATTCTAAGTATTTAGAAGAGGTTAATGTTATTGCTTTAGATTC
TATATTTTCTCCTATAGAGAAAGTTTCGTATTCTGTAGAAGATACTAGGGTTGGTCAAAGGTCAGATTATGACAAGCTTG
TAATGGAAATTTGGACTACAGGTGTGATTTCTGCCAAAGATGCAATAAAAAAGGCTGCATCAATAGTAAGAGAGTTTTTG
TTTCCTCTTGTTGATTTTGAAGACAATGTTAATACATCTTTTGAGAAATCAAAATCAGAAAGTTCTAACTTGCTTGATAT
GAGTATTGAGAAATTGAATTTGTCAGTCAGATCTTTAAATTGTTTGGCCAAAGAAAATGTTAGGACTTTAGGAGAACTTA
TTAGTAAAAACGCAGAAGAGCTTTCTAAAGCTAGAAATTTTGGGAAAAAAAGTTTGGAAGAGATAATCGAAAAACTTGGT
TCTTATCGATTATATTTAGGAATGTCTAAAGAAGATGCTCTATCTGTATTGAGTAAGAATGTTAAAATATCTGAATAA

Upstream 100 bases:

>100_bases
TTCGATTGGTATGACTGTAAAATCAATTTCAGATATTACTCCCATTCCTCATAATGGATGCAGACCGAAAAAAACCAGAC
GAGTTTAGCTAGAAGGAGTT

Downstream 100 bases:

>100_bases
AAGGAGAGTTTAGGCTATTTCATGAAAACAAAATTGGGTTTTAATAGATTAAGTAGGAAGTCTAGTCACAGGAGAGCGCT
TTTAAAAAATATGGTAATTT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MMLVEKFLKDFTIPEKIEFLKSQGDGSYGKFTIYPFERGFGITIGNTLRRVLLSSIEGYAITAMRVQSNNKDSSSKVVSS
EFDLIPGVSEDTLEIIANIKNIHLKLGEGEQRKTISFSVSGKDTNVLKASHFERDGVEVFNKDLVIATLSHDVNLDLEFQ
INYGRGYVSSEQNSKYLEEVNVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIVREFL
FPLVDFEDNVNTSFEKSKSESSNLLDMSIEKLNLSVRSLNCLAKENVRTLGELISKNAEELSKARNFGKKSLEEIIEKLG
SYRLYLGMSKEDALSVLSKNVKISE

Sequences:

>Translated_345_residues
MMLVEKFLKDFTIPEKIEFLKSQGDGSYGKFTIYPFERGFGITIGNTLRRVLLSSIEGYAITAMRVQSNNKDSSSKVVSS
EFDLIPGVSEDTLEIIANIKNIHLKLGEGEQRKTISFSVSGKDTNVLKASHFERDGVEVFNKDLVIATLSHDVNLDLEFQ
INYGRGYVSSEQNSKYLEEVNVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIVREFL
FPLVDFEDNVNTSFEKSKSESSNLLDMSIEKLNLSVRSLNCLAKENVRTLGELISKNAEELSKARNFGKKSLEEIIEKLG
SYRLYLGMSKEDALSVLSKNVKISE
>Mature_345_residues
MMLVEKFLKDFTIPEKIEFLKSQGDGSYGKFTIYPFERGFGITIGNTLRRVLLSSIEGYAITAMRVQSNNKDSSSKVVSS
EFDLIPGVSEDTLEIIANIKNIHLKLGEGEQRKTISFSVSGKDTNVLKASHFERDGVEVFNKDLVIATLSHDVNLDLEFQ
INYGRGYVSSEQNSKYLEEVNVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIVREFL
FPLVDFEDNVNTSFEKSKSESSNLLDMSIEKLNLSVRSLNCLAKENVRTLGELISKNAEELSKARNFGKKSLEEIIEKLG
SYRLYLGMSKEDALSVLSKNVKISE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=326, Percent_Identity=35.8895705521472, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_BORBU (O51455)

Other databases:

- EMBL:   AE000783
- PIR:   E70162
- RefSeq:   NP_212636.1
- ProteinModelPortal:   O51455
- EnsemblBacteria:   EBBORT00000008418
- GeneID:   1195348
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0502
- NMPDR:   fig|224326.1.peg.886
- TIGR:   BB_0502
- GeneTree:   EBGT00050000007583
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   BBUR224326:BB_0502-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38685; Mature: 38685

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLVEKFLKDFTIPEKIEFLKSQGDGSYGKFTIYPFERGFGITIGNTLRRVLLSSIEGYA
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCEE
ITAMRVQSNNKDSSSKVVSSEFDLIPGVSEDTLEIIANIKNIHLKLGEGEQRKTISFSVS
EEEEEEECCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEC
GKDTNVLKASHFERDGVEVFNKDLVIATLSHDVNLDLEFQINYGRGYVSSEQNSKYLEEV
CCCCCEEECCCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHH
NVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIVREFL
HHEEHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FPLVDFEDNVNTSFEKSKSESSNLLDMSIEKLNLSVRSLNCLAKENVRTLGELISKNAEE
HHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSKARNFGKKSLEEIIEKLGSYRLYLGMSKEDALSVLSKNVKISE
HHHHHHCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMLVEKFLKDFTIPEKIEFLKSQGDGSYGKFTIYPFERGFGITIGNTLRRVLLSSIEGYA
CHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCEE
ITAMRVQSNNKDSSSKVVSSEFDLIPGVSEDTLEIIANIKNIHLKLGEGEQRKTISFSVS
EEEEEEECCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEC
GKDTNVLKASHFERDGVEVFNKDLVIATLSHDVNLDLEFQINYGRGYVSSEQNSKYLEEV
CCCCCEEECCCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHH
NVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIVREFL
HHEEHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FPLVDFEDNVNTSFEKSKSESSNLLDMSIEKLNLSVRSLNCLAKENVRTLGELISKNAEE
HHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LSKARNFGKKSLEEIIEKLGSYRLYLGMSKEDALSVLSKNVKISE
HHHHHHCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685