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The map label for this gene is gyrB

Identifier: 15594781

GI number: 15594781

Start: 454484

End: 456403

Strand: Reverse

Name: gyrB

Synonym: BB0436

Alternate gene names: 15594781

Gene position: 456403-454484 (Counterclockwise)

Preceding gene: 161511079

Following gene: 15594780

Centisome position: 50.11

GC content: 32.08

Gene sequence:

>1920_bases
ATGAATGGAAGGTTTATGAATTATGTTGCTAGTAACATTCAGGTCTTAAAAGGACTTGAGGCTGTTAGGAAAAGGCCTGG
CATGTATATAGGCTCAGTTTCTATTAATGGATTGCACCATTTGGTTTATGAGGTGGTTGACAATAGCATTGATGAGGCTT
TAGCTGGGTTTTGTGATAGAATAGATGTTATTATCAATTTAGATAATACTATAACTGTAATTGATAATGGGAGAGGTATT
CCTACCGATATTCATGAAGAGGAGGGTATTAGTGCCCTTGAACTTGTTTTAACAAAATTACATTCTGGTGGTAAGTTTAA
TAAAGGCACGTATAAAGTTTCTGGGGGACTTCATGGCGTTGGAATTTCGGTTGTAAATGCTCTATCTTCGTTTTTAGAGG
TTTATGTTAATAGAGATGGAAAAATTTTTAGGCAAACTTTTTCAAAAGGTATTCCGACTTCTAAAGTAGAAGTTGTGGGG
GAATCTTCTGTTACGGGGACTAAGGTTACTTTTTTGGCGGATTCTGAAATTTTTGAAACTTTAGATTATAATTTCGATGT
TCTTGAAAAAAGGCTTAAAGAGCTTGCTTTTTTAAACGATAAAATATACATTTCAATTGAAGATAAAAGAATTGGTAAAG
AAAAATCTTCAAAATTTTATTTTGAGGGTGGGATAAAATCTTTTGTAGATTATTTAACTAATGACAGCAAAGCTTTTCAA
TCAGAACCTTATTATATTGATGGTTTTATTAATGATGTTATTGTTAATGTGGGGCTTAAATGGACTGAAAGCTATTCTGA
CAACATTCTTTCTTTTGTTAATAACATTAATACAAGAGAAGGGGGAACTCATGTTATGGGATTTAGAAGTGGACTTACTA
AGGCCATGAATGAAGCTTTTAAAAATTCAAAAATAAGTAAAAAAGATATTCCAAATCTTACAGGAGATGATTTTAAAGAG
GGGCTTACAGCTGTTATTTCTGTCAAAGTACCAGAACCTCAATTTGAAGGTCAAACAAAAAGTAAGCTTGGTAATTCTGA
GATAAGAAAAATAGTTGAAGTTGTTGTATATGAACATTTATTGGAAATTATTAATTTAAATCCTTTAGAGATAGACACTA
TTCTTGGAAAAGCAATAAAAGCTGCTCGTGCTCGTGAAGCTGCAAGAAAAGCAAGAGAATCAGAAAGAAAAAAAAATGCA
TTTGAAAGCTTGGCATTGCCTGGAAAATTGGCTGATTGTACTTCTAAAAATCCTTTGGAAAGAGAAATCTATATTGTAGA
AGGTGATTCTGCTGGAGGAAGTGCTAAAATGGGTAGAAATAGATTTTTTCAGGCCATTTTGCCACTGTGGGGGAAAATGC
TTAATGTTGAGAAAACAAGAGAAGATAAGGTTATCACCAATGATAAGCTTATTCCCATAATTGCATCTCTTGGTGCAGGA
GTTGGTAAAACTTTTGATATTACAAAACTTCGTTATCACAAGATCATTATTATGGCAGATGCCGATGTTGATGGATCTCA
TATTAGAACTTTGCTTTTAGCTTTTTTCTTTAGATATATGAGAGATTTAATTGAAAATGGATATATATATATAGCCATGC
CTCCTCTTTATAAAATAAAGTATGACAATCGTATTTATTATTTTTATGAAGAGAAAGAAAAAGAAAAATTTTTAGATTCT
ATTGAAACTAAAAATCGCAATAGTATTTCTCTTCAGAGATATAAAGGGCTTGGGGAGATGAATCCAACGCAGCTTTGGGA
AACAACTATGGATCCTGCTAGAAGAAAAATGAGATTGATGAATATAGATGATGCTATTGAAGCTGAAAAAATTTTTGTTA
CTCTTATGGGAGATTTAGTTGAGCCCAGAAAAGAATTTATTGAACAGAATGCACTTAATGTAATTAATCTTGATGTGTAA

Upstream 100 bases:

>100_bases
TAAATTGTTTGTGAATTTTTAAAAGTTTTTGGTATTTCCTATGTAATAGATATTATTTTTTTTTTAATGTAGAATTTATC
TGTGTCTAGATTTTAATTAA

Downstream 100 bases:

>100_bases
TTGGAGCGTTAATGGCAGTTGGAGAAAATAAAGAACAAATATTAAATGTTAGGATAGAAGATGAAATAAAAACTTCTTAT
TTAAATTATGCAATGTCAGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MNGRFMNYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEVVDNSIDEALAGFCDRIDVIINLDNTITVIDNGRGI
PTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGVGISVVNALSSFLEVYVNRDGKIFRQTFSKGIPTSKVEVVG
ESSVTGTKVTFLADSEIFETLDYNFDVLEKRLKELAFLNDKIYISIEDKRIGKEKSSKFYFEGGIKSFVDYLTNDSKAFQ
SEPYYIDGFINDVIVNVGLKWTESYSDNILSFVNNINTREGGTHVMGFRSGLTKAMNEAFKNSKISKKDIPNLTGDDFKE
GLTAVISVKVPEPQFEGQTKSKLGNSEIRKIVEVVVYEHLLEIINLNPLEIDTILGKAIKAARAREAARKARESERKKNA
FESLALPGKLADCTSKNPLEREIYIVEGDSAGGSAKMGRNRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
VGKTFDITKLRYHKIIIMADADVDGSHIRTLLLAFFFRYMRDLIENGYIYIAMPPLYKIKYDNRIYYFYEEKEKEKFLDS
IETKNRNSISLQRYKGLGEMNPTQLWETTMDPARRKMRLMNIDDAIEAEKIFVTLMGDLVEPRKEFIEQNALNVINLDV

Sequences:

>Translated_639_residues
MNGRFMNYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEVVDNSIDEALAGFCDRIDVIINLDNTITVIDNGRGI
PTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGVGISVVNALSSFLEVYVNRDGKIFRQTFSKGIPTSKVEVVG
ESSVTGTKVTFLADSEIFETLDYNFDVLEKRLKELAFLNDKIYISIEDKRIGKEKSSKFYFEGGIKSFVDYLTNDSKAFQ
SEPYYIDGFINDVIVNVGLKWTESYSDNILSFVNNINTREGGTHVMGFRSGLTKAMNEAFKNSKISKKDIPNLTGDDFKE
GLTAVISVKVPEPQFEGQTKSKLGNSEIRKIVEVVVYEHLLEIINLNPLEIDTILGKAIKAARAREAARKARESERKKNA
FESLALPGKLADCTSKNPLEREIYIVEGDSAGGSAKMGRNRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
VGKTFDITKLRYHKIIIMADADVDGSHIRTLLLAFFFRYMRDLIENGYIYIAMPPLYKIKYDNRIYYFYEEKEKEKFLDS
IETKNRNSISLQRYKGLGEMNPTQLWETTMDPARRKMRLMNIDDAIEAEKIFVTLMGDLVEPRKEFIEQNALNVINLDV
>Mature_639_residues
MNGRFMNYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEVVDNSIDEALAGFCDRIDVIINLDNTITVIDNGRGI
PTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGVGISVVNALSSFLEVYVNRDGKIFRQTFSKGIPTSKVEVVG
ESSVTGTKVTFLADSEIFETLDYNFDVLEKRLKELAFLNDKIYISIEDKRIGKEKSSKFYFEGGIKSFVDYLTNDSKAFQ
SEPYYIDGFINDVIVNVGLKWTESYSDNILSFVNNINTREGGTHVMGFRSGLTKAMNEAFKNSKISKKDIPNLTGDDFKE
GLTAVISVKVPEPQFEGQTKSKLGNSEIRKIVEVVVYEHLLEIINLNPLEIDTILGKAIKAARAREAARKARESERKKNA
FESLALPGKLADCTSKNPLEREIYIVEGDSAGGSAKMGRNRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
VGKTFDITKLRYHKIIIMADADVDGSHIRTLLLAFFFRYMRDLIENGYIYIAMPPLYKIKYDNRIYYFYEEKEKEKFLDS
IETKNRNSISLQRYKGLGEMNPTQLWETTMDPARRKMRLMNIDDAIEAEKIFVTLMGDLVEPRKEFIEQNALNVINLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI19913408, Length=625, Percent_Identity=24.8, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI19913406, Length=642, Percent_Identity=23.3644859813084, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=51.7921146953405, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1789408, Length=601, Percent_Identity=41.7637271214642, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17535065, Length=640, Percent_Identity=25, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=606, Percent_Identity=26.2376237623762, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=197, Percent_Identity=27.9187817258883, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=618, Percent_Identity=27.0226537216829, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136538, Length=655, Percent_Identity=24.5801526717557, Blast_Score=136, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_BORBU (P33769)

Other databases:

- EMBL:   AE000783
- EMBL:   U04527
- EMBL:   AF017075
- EMBL:   Z12166
- PIR:   C70154
- RefSeq:   NP_212570.1
- ProteinModelPortal:   P33769
- EnsemblBacteria:   EBBORT00000008551
- GeneID:   1195281
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0436
- NMPDR:   fig|224326.1.peg.820
- TIGR:   BB_0436
- GeneTree:   EBGT00050000007199
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- PhylomeDB:   P33769
- ProtClustDB:   PRK05644
- BioCyc:   BBUR224326:BB_0436-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 72058; Mature: 72058

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGRFMNYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEVVDNSIDEALAGFCDR
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHCC
IDVIINLDNTITVIDNGRGIPTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGV
EEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHH
GISVVNALSSFLEVYVNRDGKIFRQTFSKGIPTSKVEVVGESSVTGTKVTFLADSEIFET
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECHHHHHH
LDYNFDVLEKRLKELAFLNDKIYISIEDKRIGKEKSSKFYFEGGIKSFVDYLTNDSKAFQ
HCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHC
SEPYYIDGFINDVIVNVGLKWTESYSDNILSFVNNINTREGGTHVMGFRSGLTKAMNEAF
CCCCEECHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
KNSKISKKDIPNLTGDDFKEGLTAVISVKVPEPQFEGQTKSKLGNSEIRKIVEVVVYEHL
HCCCCCHHCCCCCCCCHHHCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
LEIINLNPLEIDTILGKAIKAARAREAARKARESERKKNAFESLALPGKLADCTSKNPLE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
REIYIVEGDSAGGSAKMGRNRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHCCC
VGKTFDITKLRYHKIIIMADADVDGSHIRTLLLAFFFRYMRDLIENGYIYIAMPPLYKIK
CCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEE
YDNRIYYFYEEKEKEKFLDSIETKNRNSISLQRYKGLGEMNPTQLWETTMDPARRKMRLM
ECCEEEEEEECHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHHEECC
NIDDAIEAEKIFVTLMGDLVEPRKEFIEQNALNVINLDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MNGRFMNYVASNIQVLKGLEAVRKRPGMYIGSVSINGLHHLVYEVVDNSIDEALAGFCDR
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCHHHHHHHHHHCC
IDVIINLDNTITVIDNGRGIPTDIHEEEGISALELVLTKLHSGGKFNKGTYKVSGGLHGV
EEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHH
GISVVNALSSFLEVYVNRDGKIFRQTFSKGIPTSKVEVVGESSVTGTKVTFLADSEIFET
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECHHHHHH
LDYNFDVLEKRLKELAFLNDKIYISIEDKRIGKEKSSKFYFEGGIKSFVDYLTNDSKAFQ
HCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHC
SEPYYIDGFINDVIVNVGLKWTESYSDNILSFVNNINTREGGTHVMGFRSGLTKAMNEAF
CCCCEECHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
KNSKISKKDIPNLTGDDFKEGLTAVISVKVPEPQFEGQTKSKLGNSEIRKIVEVVVYEHL
HCCCCCHHCCCCCCCCHHHCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
LEIINLNPLEIDTILGKAIKAARAREAARKARESERKKNAFESLALPGKLADCTSKNPLE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
REIYIVEGDSAGGSAKMGRNRFFQAILPLWGKMLNVEKTREDKVITNDKLIPIIASLGAG
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHCCC
VGKTFDITKLRYHKIIIMADADVDGSHIRTLLLAFFFRYMRDLIENGYIYIAMPPLYKIK
CCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEE
YDNRIYYFYEEKEKEKFLDSIETKNRNSISLQRYKGLGEMNPTQLWETTMDPARRKMRLM
ECCEEEEEEECHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHHEECC
NIDDAIEAEKIFVTLMGDLVEPRKEFIEQNALNVINLDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 8359672; 8188609; 1490605