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The map label for this gene is gyrA

Identifier: 15594780

GI number: 15594780

Start: 452040

End: 454472

Strand: Reverse

Name: gyrA

Synonym: BB0435

Alternate gene names: 15594780

Gene position: 454472-452040 (Counterclockwise)

Preceding gene: 15594781

Following gene: 15594779

Centisome position: 49.9

GC content: 30.91

Gene sequence:

>2433_bases
ATGGCAGTTGGAGAAAATAAAGAACAAATATTAAATGTTAGGATAGAAGATGAAATAAAAACTTCTTATTTAAATTATGC
AATGTCAGTTATTGTTTCTAGAGCTCTTCCAGACGTAAGAGATGGTCTTAAACCAGTTCACAGGAGAATACTTTATTCTA
TGTATGAGATGGGACTTCGTTCTGATAAGGCTTTTAAAAAAGCTGGTAGAATAGTGGGAGATGTTCTTGGGAAATATCAT
CCTCATGGAGATCAATCAATTTATGATGCTCTTGTAAGACTTGCTCAGGATTTTTCGCTTAGATATCCCGTAATACGGGG
ACAGGGAAATTTTGGATCTATTGACGGAGATCCCCCCGCTGCTATGAGATATACTGAAGCTAAAATGGAAAAAATAACTG
AATATATTGTTAAGGATATAGACAAAGAGACTGTTAATTTTAAGTCTAATTATGACGATTCTTTAAGTGAGCCTGAGATT
ATGCCGTCATCATTTCCATTTCTTTTGGTAAATGGCTCTAGTGGAATTGCTGTTGGAATGGCTACTAATATGGCACCTCA
TAATTTAAGAGAAATTTGTGATGCCATTGTTTACATGCTAGATAATGAGAATGCTTCTATATTTGATTTGCTTAAAATAG
TTAAAGGGCCTGATTTCCCAACTTTTGGAGAGATTGTTTATAATGATAATTTAATTAAAGCATACAAAACTGGCAAGGGA
AGTGTTGTTATTAGGGCAAGATATCATATTGAAGAAAGAGCAGAAGATAGAAATGCTATAATTGTTACAGAAATACCTTA
TACGGTAAATAAATCTGCACTTCTTATGAAAGTTGCGCTTTTAGCAAAAGAAGAAAAGCTAGAAGGACTTTTAGATATAA
GAGATGAATCTGATCGAGAAGGTATTAGGATAGTTCTTGAAGTTAAAAGAGGATTTGATCCTCATGTTATTATGAATTTG
CTTTATGAATATACTGAATTTAAAAAGCATTTTAGTATAAATAATTTAGCCCTTGTTAATGGTATTCCCAAACAGTTAAA
TTTAGAAGAATTGTTATTTGAATTTATTGAGCATAGAAAAAATATTATCGAAAGACGTATTGAATTTGACTTGAGAAAGG
CAAAAGAGAAAGCACATGTTCTTGAGGGATTAAATATTGCTTTAAATAATATAGATGAGGTTATTAAGATTATTAAATCA
TCTAAATTAGCAAAAGATGCAAGGGAGAGGCTTGTTTCGAATTTTGGTCTTTCAGAGATTCAGGCCAATTCAGTTCTTGA
TATGAGGTTACAAAAACTTACAGCCCTTGAGATTTTTAAGCTTGAAGAGGAGCTTAATATACTGTTAAGCTTAATAAAAG
ATTATGAAGATATTCTCTTGAATCCAGTAAGGATTATTAATATTATAAGAGAAGAAACTATTAATTTAGGTTTGAAATTT
GGCGATGAACGTCGAACTAAAATAATTTATGATGAGGAGGTTTTAAAAACTAGTATGTCGGATTTAATGCAAAAAGAAAA
TATTGTTGTTATGCTTACAAAGAAAGGTTTCCTTAAAAGACTTTCACAAAATGAGTATAAATTGCAAGGTACGGGAGGAA
AAGGACTAAGTTCGTTTGATCTAAATGATGGAGATGAGATTGTTATTGCTTTGTGTGTCAATACTCATGATTATTTATTT
ATGATTTCAAATGAAGGAAAGCTTTATTTAATCAATGCTTATGAAATAAAAGATTCTTCAAGAGCTTCAAAAGGTCAGAA
TATTAGTGAGCTTATTAATTTAGGAGATCAAGAAGAAATATTAACTATTAAGAATAGTAAAGATTTAACTGATGATGCTT
ATTTATTGCTTACAACTGCAAGTGGAAAGATAGCTAGATTCGAATCTACAGATTTTAAAGCAGTAAAGTCACGAGGTGTT
ATTGTTATTAAACTGAATGATAAAGATTTTGTTACAAGTGCAGAGATTGTTTTTAAGGATGAAAAAGTAATTTGTCTTTC
TAAAAAGGGTAGTGCATTTATATTTAATTCAAGGGATGTTAGGCTTACTAATAGAGGTACCCAAGGTGTTTGTGGAATGA
AATTAAAAGAAGGTGATTTGTTTGTTAAAGTTTTATCGGTTAAAGAAAATCCTTATCTTTTGATTGTTTCTGAAAATGGG
TATGGAAAAAGGTTAAACATGTCTAAAATATCTGAGCTTAAAAGAGGAGCCACTGGTTATACTAGTTATAAAAAATCTGA
TAAAAAAGCGGGTAGTGTTGTTGATGCTATAGCAGTTTCAGAGGATGATGAAATCTTGCTTGTAAGTAAACGTTCAAAAG
CTTTAAGAACAGTAGCTGGAAAAGTATCTGAACAAGGCAAAGATGCTAGAGGAATTCAAGTATTATTTCTTGATAATGAC
AGCTTGGTTTCTGTTTCAAAATTTATTAAATAA

Upstream 100 bases:

>100_bases
TTTTTGTTACTCTTATGGGAGATTTAGTTGAGCCCAGAAAAGAATTTATTGAACAGAATGCACTTAATGTAATTAATCTT
GATGTGTAATTGGAGCGTTA

Downstream 100 bases:

>100_bases
AGAATTGGTTTTTCTTGTTTAAGAATGTTCCACGTGGAACATTCTTTTTTTTTATTCTTAAATTGTAATTCAATATTATA
ACCTGGTAAAATTTTGTGTT

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MAVGENKEQILNVRIEDEIKTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMYEMGLRSDKAFKKAGRIVGDVLGKYH
PHGDQSIYDALVRLAQDFSLRYPVIRGQGNFGSIDGDPPAAMRYTEAKMEKITEYIVKDIDKETVNFKSNYDDSLSEPEI
MPSSFPFLLVNGSSGIAVGMATNMAPHNLREICDAIVYMLDNENASIFDLLKIVKGPDFPTFGEIVYNDNLIKAYKTGKG
SVVIRARYHIEERAEDRNAIIVTEIPYTVNKSALLMKVALLAKEEKLEGLLDIRDESDREGIRIVLEVKRGFDPHVIMNL
LYEYTEFKKHFSINNLALVNGIPKQLNLEELLFEFIEHRKNIIERRIEFDLRKAKEKAHVLEGLNIALNNIDEVIKIIKS
SKLAKDARERLVSNFGLSEIQANSVLDMRLQKLTALEIFKLEEELNILLSLIKDYEDILLNPVRIINIIREETINLGLKF
GDERRTKIIYDEEVLKTSMSDLMQKENIVVMLTKKGFLKRLSQNEYKLQGTGGKGLSSFDLNDGDEIVIALCVNTHDYLF
MISNEGKLYLINAYEIKDSSRASKGQNISELINLGDQEEILTIKNSKDLTDDAYLLLTTASGKIARFESTDFKAVKSRGV
IVIKLNDKDFVTSAEIVFKDEKVICLSKKGSAFIFNSRDVRLTNRGTQGVCGMKLKEGDLFVKVLSVKENPYLLIVSENG
YGKRLNMSKISELKRGATGYTSYKKSDKKAGSVVDAIAVSEDDEILLVSKRSKALRTVAGKVSEQGKDARGIQVLFLDND
SLVSVSKFIK

Sequences:

>Translated_810_residues
MAVGENKEQILNVRIEDEIKTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMYEMGLRSDKAFKKAGRIVGDVLGKYH
PHGDQSIYDALVRLAQDFSLRYPVIRGQGNFGSIDGDPPAAMRYTEAKMEKITEYIVKDIDKETVNFKSNYDDSLSEPEI
MPSSFPFLLVNGSSGIAVGMATNMAPHNLREICDAIVYMLDNENASIFDLLKIVKGPDFPTFGEIVYNDNLIKAYKTGKG
SVVIRARYHIEERAEDRNAIIVTEIPYTVNKSALLMKVALLAKEEKLEGLLDIRDESDREGIRIVLEVKRGFDPHVIMNL
LYEYTEFKKHFSINNLALVNGIPKQLNLEELLFEFIEHRKNIIERRIEFDLRKAKEKAHVLEGLNIALNNIDEVIKIIKS
SKLAKDARERLVSNFGLSEIQANSVLDMRLQKLTALEIFKLEEELNILLSLIKDYEDILLNPVRIINIIREETINLGLKF
GDERRTKIIYDEEVLKTSMSDLMQKENIVVMLTKKGFLKRLSQNEYKLQGTGGKGLSSFDLNDGDEIVIALCVNTHDYLF
MISNEGKLYLINAYEIKDSSRASKGQNISELINLGDQEEILTIKNSKDLTDDAYLLLTTASGKIARFESTDFKAVKSRGV
IVIKLNDKDFVTSAEIVFKDEKVICLSKKGSAFIFNSRDVRLTNRGTQGVCGMKLKEGDLFVKVLSVKENPYLLIVSENG
YGKRLNMSKISELKRGATGYTSYKKSDKKAGSVVDAIAVSEDDEILLVSKRSKALRTVAGKVSEQGKDARGIQVLFLDND
SLVSVSKFIK
>Mature_809_residues
AVGENKEQILNVRIEDEIKTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMYEMGLRSDKAFKKAGRIVGDVLGKYHP
HGDQSIYDALVRLAQDFSLRYPVIRGQGNFGSIDGDPPAAMRYTEAKMEKITEYIVKDIDKETVNFKSNYDDSLSEPEIM
PSSFPFLLVNGSSGIAVGMATNMAPHNLREICDAIVYMLDNENASIFDLLKIVKGPDFPTFGEIVYNDNLIKAYKTGKGS
VVIRARYHIEERAEDRNAIIVTEIPYTVNKSALLMKVALLAKEEKLEGLLDIRDESDREGIRIVLEVKRGFDPHVIMNLL
YEYTEFKKHFSINNLALVNGIPKQLNLEELLFEFIEHRKNIIERRIEFDLRKAKEKAHVLEGLNIALNNIDEVIKIIKSS
KLAKDARERLVSNFGLSEIQANSVLDMRLQKLTALEIFKLEEELNILLSLIKDYEDILLNPVRIINIIREETINLGLKFG
DERRTKIIYDEEVLKTSMSDLMQKENIVVMLTKKGFLKRLSQNEYKLQGTGGKGLSSFDLNDGDEIVIALCVNTHDYLFM
ISNEGKLYLINAYEIKDSSRASKGQNISELINLGDQEEILTIKNSKDLTDDAYLLLTTASGKIARFESTDFKAVKSRGVI
VIKLNDKDFVTSAEIVFKDEKVICLSKKGSAFIFNSRDVRLTNRGTQGVCGMKLKEGDLFVKVLSVKENPYLLIVSENGY
GKRLNMSKISELKRGATGYTSYKKSDKKAGSVVDAIAVSEDDEILLVSKRSKALRTVAGKVSEQGKDARGIQVLFLDNDS
LVSVSKFIK

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family

Homologues:

Organism=Homo sapiens, GI19913408, Length=275, Percent_Identity=26.5454545454545, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI19913406, Length=177, Percent_Identity=30.5084745762712, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788562, Length=819, Percent_Identity=40.9035409035409, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=675, Percent_Identity=34.0740740740741, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=310, Percent_Identity=25.4838709677419, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI212645657, Length=322, Percent_Identity=24.8447204968944, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI212645845, Length=451, Percent_Identity=25.7206208425721, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115534553, Length=172, Percent_Identity=29.0697674418605, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=366, Percent_Identity=25.1366120218579, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17136538, Length=180, Percent_Identity=29.4444444444444, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRA_BORBU (O51396)

Other databases:

- EMBL:   AE000783
- EMBL:   Z12165
- PIR:   B70154
- RefSeq:   NP_212569.1
- PDB:   1SUU
- PDBsum:   1SUU
- ProteinModelPortal:   O51396
- EnsemblBacteria:   EBBORT00000009021
- GeneID:   1195280
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0435
- NMPDR:   fig|224326.1.peg.819
- TIGR:   BB_0435
- GeneTree:   EBGT00050000007271
- HOGENOM:   HBG635183
- OMA:   QRENVIV
- ProtClustDB:   PRK05560
- BioCyc:   BBUR224326:BB_0435-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760
- Gene3D:   G3DSA:3.30.1360.40
- Gene3D:   G3DSA:3.90.199.10
- Gene3D:   G3DSA:1.10.268.10
- SMART:   SM00434
- TIGRFAMs:   TIGR01063

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 91381; Mature: 91250

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: ACT_SITE 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGENKEQILNVRIEDEIKTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMYEMGLR
CCCCCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
SDKAFKKAGRIVGDVLGKYHPHGDQSIYDALVRLAQDFSLRYPVIRGQGNFGSIDGDPPA
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCH
AMRYTEAKMEKITEYIVKDIDKETVNFKSNYDDSLSEPEIMPSSFPFLLVNGSSGIAVGM
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEE
ATNMAPHNLREICDAIVYMLDNENASIFDLLKIVKGPDFPTFGEIVYNDNLIKAYKTGKG
ECCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCC
SVVIRARYHIEERAEDRNAIIVTEIPYTVNKSALLMKVALLAKEEKLEGLLDIRDESDRE
CEEEEEEECHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCC
GIRIVLEVKRGFDPHVIMNLLYEYTEFKKHFSINNLALVNGIPKQLNLEELLFEFIEHRK
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
NIIERRIEFDLRKAKEKAHVLEGLNIALNNIDEVIKIIKSSKLAKDARERLVSNFGLSEI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QANSVLDMRLQKLTALEIFKLEEELNILLSLIKDYEDILLNPVRIINIIREETINLGLKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEE
GDERRTKIIYDEEVLKTSMSDLMQKENIVVMLTKKGFLKRLSQNEYKLQGTGGKGLSSFD
CCCCCEEEEECHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCEEEEECCCCCCCCCC
LNDGDEIVIALCVNTHDYLFMISNEGKLYLINAYEIKDSSRASKGQNISELINLGDQEEI
CCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCCCCCE
LTIKNSKDLTDDAYLLLTTASGKIARFESTDFKAVKSRGVIVIKLNDKDFVTSAEIVFKD
EEEECCCCCCCCEEEEEEECCCCEEEECCCCHHHHHCCCEEEEEECCCCCEEEEEEEEEC
EKVICLSKKGSAFIFNSRDVRLTNRGTQGVCGMKLKEGDLFVKVLSVKENPYLLIVSENG
CEEEEEECCCCEEEECCCCEEEECCCCCCCCCEEEECCCEEEEEEEECCCCEEEEEECCC
YGKRLNMSKISELKRGATGYTSYKKSDKKAGSVVDAIAVSEDDEILLVSKRSKALRTVAG
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCEEEEECCHHHHHHHHH
KVSEQGKDARGIQVLFLDNDSLVSVSKFIK
HHHHCCCCCCCEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
AVGENKEQILNVRIEDEIKTSYLNYAMSVIVSRALPDVRDGLKPVHRRILYSMYEMGLR
CCCCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
SDKAFKKAGRIVGDVLGKYHPHGDQSIYDALVRLAQDFSLRYPVIRGQGNFGSIDGDPPA
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCH
AMRYTEAKMEKITEYIVKDIDKETVNFKSNYDDSLSEPEIMPSSFPFLLVNGSSGIAVGM
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEE
ATNMAPHNLREICDAIVYMLDNENASIFDLLKIVKGPDFPTFGEIVYNDNLIKAYKTGKG
ECCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCC
SVVIRARYHIEERAEDRNAIIVTEIPYTVNKSALLMKVALLAKEEKLEGLLDIRDESDRE
CEEEEEEECHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCC
GIRIVLEVKRGFDPHVIMNLLYEYTEFKKHFSINNLALVNGIPKQLNLEELLFEFIEHRK
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
NIIERRIEFDLRKAKEKAHVLEGLNIALNNIDEVIKIIKSSKLAKDARERLVSNFGLSEI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QANSVLDMRLQKLTALEIFKLEEELNILLSLIKDYEDILLNPVRIINIIREETINLGLKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEE
GDERRTKIIYDEEVLKTSMSDLMQKENIVVMLTKKGFLKRLSQNEYKLQGTGGKGLSSFD
CCCCCEEEEECHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCEEEEECCCCCCCCCC
LNDGDEIVIALCVNTHDYLFMISNEGKLYLINAYEIKDSSRASKGQNISELINLGDQEEI
CCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCCCCCE
LTIKNSKDLTDDAYLLLTTASGKIARFESTDFKAVKSRGVIVIKLNDKDFVTSAEIVFKD
EEEECCCCCCCCEEEEEEECCCCEEEECCCCHHHHHCCCEEEEEECCCCCEEEEEEEEEC
EKVICLSKKGSAFIFNSRDVRLTNRGTQGVCGMKLKEGDLFVKVLSVKENPYLLIVSENG
CEEEEEECCCCEEEECCCCEEEECCCCCCCCCEEEECCCEEEEEEEECCCCEEEEEECCC
YGKRLNMSKISELKRGATGYTSYKKSDKKAGSVVDAIAVSEDDEILLVSKRSKALRTVAG
CCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCEEEEECCHHHHHHHHH
KVSEQGKDARGIQVLFLDNDSLVSVSKFIK
HHHHCCCCCCCEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 1490605