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The map label for this gene is hslU [H]

Identifier: 15594640

GI number: 15594640

Start: 303774

End: 305141

Strand: Reverse

Name: hslU [H]

Synonym: BB0295

Alternate gene names: 15594640

Gene position: 305141-303774 (Counterclockwise)

Preceding gene: 15594641

Following gene: 15594639

Centisome position: 33.51

GC content: 30.41

Gene sequence:

>1368_bases
TTGTGCTTGAGGAGATTGAAAATGAATAAATTAGAAGAGCACTATATAGTTCCCAAAGATGTAGTTGCAGAACTTGATAA
ATATATAATAGGTCAAGACGAAGCTAAAAAATTAGTATCAATTGCTCTTGTTAATAGATATATAAGGTCTAGGCTTCCAA
AAGAAATAAAAGATGAGGTAATGCCTAAAAACATTATTATGATTGGATCAACTGGCATTGGGAAGACCGAGATTGCAAGA
AGACTTTCTAAATTAATTAAAGCTCCTTTTATTAAAGTTGAGGCTACAAAATATACTGAGGTTGGTTATGTTGGTCGTGA
TGTTGAATCTATGGTTAGAGATTTAATGAGCATTGCAGTTAATATGGTAAAAGAAGAGATGTATAGTACTGTAAGAGATG
ATGCTTTAGTAAGAACAGAGGAGAGAATAGTTGATAGTCTTTTTAAGGGATCTAGTAATTCTGAGAATATGGATCCAAAT
GAAATAAAGGCGGAAGAAAAGGTAAAAGAGAAGCTTAGAAAAAAGCTTAGAGCAGGTGAGCTTGATGATACTACTATTGA
AATACAAATTTCTAGTAAAATGCCATTTTCTACAATAGAAATATTTACGGGTGGTAATTTTGAAGAGATTGATATGGGAA
TTGGCGGTTTGCTGGGTAATATATTTGATAGAAAAAAGAAAAGAGAATTGAAGATTAAAAAAGCAAAGGAAATAATATTA
GCAGAAGAGCTTGAGAAATTGGTCGATCACGAAAACATTTCAGATATTGCAAAATCTAAAGTTGAAAATATGGGAATTAT
TTTTATTGATGAGATCGATAAAATAGCTGCTAAGAATAGGAGTGGCAATGATGTGTCCAGAGAAGGTGTTCAAAGAGATA
TTTTGCCAATTATTGAAGGTTCTAAAGTTAATACAAAATATGGAATAGTTGATACTTCTCATATTTTATTTATTGCAGCA
GGAGCATTTAATTTAGCTAAACCTTCTGATTTAATACCCGAGCTTCAAGGAAGATTTCCTATTAAGGTTGAACTTAAGAG
CTTAAGCATAGACGATTTGAAAAAAATTTTAAAACAAACAAAAAATTCTTTAATAAAACAATATGTTGCGATGTTTAAGG
TTTATGATTTAGATTTAAAGTTTAGTGAGGAAGCTATAGATAGAATTGCAGAGCTTACTTTTAATATGAATCTTGAGAGT
GAAAATCTTGGTGCCAGAAGACTTCACGGTGTTATGGAAATAGTGCTTGCAGATCTTTTTTTTGAAGTGCCTGGCAGTAA
GTTGAAAAAATTTGAAATAAACTTGGACTATGTTAATAAAAAAATACAAATTAACGAACAAAAAGATTTGAATTATTATA
TAATTTAG

Upstream 100 bases:

>100_bases
GCTCTTGCTTACATGGAGAACAAAAAATTAAGCGCTTTTGAGGTTGCACTTAGATCTTTAAAAATAGCAGCAAGAGTGTG
TATATATACTAATTCTAATA

Downstream 100 bases:

>100_bases
TTAAAAGCAATTTTAAATGAGGGAGGTTTCAATTTGAATGATTTTGAAAGATCTGTAGATTTTTCACATAGGTATTTAGA
TGTTTTGAGTTTAAGACAAA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MCLRRLKMNKLEEHYIVPKDVVAELDKYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEVMPKNIIMIGSTGIGKTEIAR
RLSKLIKAPFIKVEATKYTEVGYVGRDVESMVRDLMSIAVNMVKEEMYSTVRDDALVRTEERIVDSLFKGSSNSENMDPN
EIKAEEKVKEKLRKKLRAGELDDTTIEIQISSKMPFSTIEIFTGGNFEEIDMGIGGLLGNIFDRKKKRELKIKKAKEIIL
AEELEKLVDHENISDIAKSKVENMGIIFIDEIDKIAAKNRSGNDVSREGVQRDILPIIEGSKVNTKYGIVDTSHILFIAA
GAFNLAKPSDLIPELQGRFPIKVELKSLSIDDLKKILKQTKNSLIKQYVAMFKVYDLDLKFSEEAIDRIAELTFNMNLES
ENLGARRLHGVMEIVLADLFFEVPGSKLKKFEINLDYVNKKIQINEQKDLNYYII

Sequences:

>Translated_455_residues
MCLRRLKMNKLEEHYIVPKDVVAELDKYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEVMPKNIIMIGSTGIGKTEIAR
RLSKLIKAPFIKVEATKYTEVGYVGRDVESMVRDLMSIAVNMVKEEMYSTVRDDALVRTEERIVDSLFKGSSNSENMDPN
EIKAEEKVKEKLRKKLRAGELDDTTIEIQISSKMPFSTIEIFTGGNFEEIDMGIGGLLGNIFDRKKKRELKIKKAKEIIL
AEELEKLVDHENISDIAKSKVENMGIIFIDEIDKIAAKNRSGNDVSREGVQRDILPIIEGSKVNTKYGIVDTSHILFIAA
GAFNLAKPSDLIPELQGRFPIKVELKSLSIDDLKKILKQTKNSLIKQYVAMFKVYDLDLKFSEEAIDRIAELTFNMNLES
ENLGARRLHGVMEIVLADLFFEVPGSKLKKFEINLDYVNKKIQINEQKDLNYYII
>Mature_455_residues
MCLRRLKMNKLEEHYIVPKDVVAELDKYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEVMPKNIIMIGSTGIGKTEIAR
RLSKLIKAPFIKVEATKYTEVGYVGRDVESMVRDLMSIAVNMVKEEMYSTVRDDALVRTEERIVDSLFKGSSNSENMDPN
EIKAEEKVKEKLRKKLRAGELDDTTIEIQISSKMPFSTIEIFTGGNFEEIDMGIGGLLGNIFDRKKKRELKIKKAKEIIL
AEELEKLVDHENISDIAKSKVENMGIIFIDEIDKIAAKNRSGNDVSREGVQRDILPIIEGSKVNTKYGIVDTSHILFIAA
GAFNLAKPSDLIPELQGRFPIKVELKSLSIDDLKKILKQTKNSLIKQYVAMFKVYDLDLKFSEEAIDRIAELTFNMNLES
ENLGARRLHGVMEIVLADLFFEVPGSKLKKFEINLDYVNKKIQINEQKDLNYYII

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=111, Percent_Identity=36.036036036036, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790366, Length=444, Percent_Identity=47.0720720720721, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1786642, Length=215, Percent_Identity=34.4186046511628, Blast_Score=99, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982908, Length=287, Percent_Identity=29.2682926829268, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982905, Length=287, Percent_Identity=29.2682926829268, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=34.7517730496454, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648289, Length=241, Percent_Identity=31.1203319502075, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648291, Length=241, Percent_Identity=31.1203319502075, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR011704
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07724 AAA_2; PF07728 AAA_5; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 51883; Mature: 51883

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLRRLKMNKLEEHYIVPKDVVAELDKYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEV
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
MPKNIIMIGSTGIGKTEIARRLSKLIKAPFIKVEATKYTEVGYVGRDVESMVRDLMSIAV
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
NMVKEEMYSTVRDDALVRTEERIVDSLFKGSSNSENMDPNEIKAEEKVKEKLRKKLRAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LDDTTIEIQISSKMPFSTIEIFTGGNFEEIDMGIGGLLGNIFDRKKKRELKIKKAKEIIL
CCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEELEKLVDHENISDIAKSKVENMGIIFIDEIDKIAAKNRSGNDVSREGVQRDILPIIEG
HHHHHHHHCCCCHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
SKVNTKYGIVDTSHILFIAAGAFNLAKPSDLIPELQGRFPIKVELKSLSIDDLKKILKQT
CCCCCEECCEECCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
KNSLIKQYVAMFKVYDLDLKFSEEAIDRIAELTFNMNLESENLGARRLHGVMEIVLADLF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FEVPGSKLKKFEINLDYVNKKIQINEQKDLNYYII
HHCCCCCCEEEEEEHHHCCCEEEECCCCCCCEEEC
>Mature Secondary Structure
MCLRRLKMNKLEEHYIVPKDVVAELDKYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEV
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHC
MPKNIIMIGSTGIGKTEIARRLSKLIKAPFIKVEATKYTEVGYVGRDVESMVRDLMSIAV
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
NMVKEEMYSTVRDDALVRTEERIVDSLFKGSSNSENMDPNEIKAEEKVKEKLRKKLRAGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LDDTTIEIQISSKMPFSTIEIFTGGNFEEIDMGIGGLLGNIFDRKKKRELKIKKAKEIIL
CCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEELEKLVDHENISDIAKSKVENMGIIFIDEIDKIAAKNRSGNDVSREGVQRDILPIIEG
HHHHHHHHCCCCHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
SKVNTKYGIVDTSHILFIAAGAFNLAKPSDLIPELQGRFPIKVELKSLSIDDLKKILKQT
CCCCCEECCEECCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
KNSLIKQYVAMFKVYDLDLKFSEEAIDRIAELTFNMNLESENLGARRLHGVMEIVLADLF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
FEVPGSKLKKFEINLDYVNKKIQINEQKDLNYYII
HHCCCCCCEEEEEEHHHCCCEEEECCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]