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The map label for this gene is fliF

Identifier: 15594636

GI number: 15594636

Start: 300780

End: 302489

Strand: Reverse

Name: fliF

Synonym: BB0291

Alternate gene names: 15594636

Gene position: 302489-300780 (Counterclockwise)

Preceding gene: 15594637

Following gene: 15594635

Centisome position: 33.21

GC content: 33.86

Gene sequence:

>1710_bases
TTGAGCAAATTTTTTACTAATTTTTTTGTTTCAGCAAAAGGAATCTTCAAAAAAGCTAGTACGGTTCAGAAAATAGCCTT
AGGATTGATTATTTTTTTTGTGATTCTTGCGCTTGTTTTTTTAATAGGGTTTTCTACTAAAAGTCAAAGCATTGCTCTTT
TTGGGGTTGAAATTAAAGATCAATATCTCTTAGATAGGATATCGCAAAGACTTGATAGGGAAAATGTTAAGTATTTTTTG
AGTTCTGATGGAAGAATTTATTTAGATGATGAAAAGCTTGCAAAAAAAATGAGAGCAATTCTTGTCAGAGAAGAGCTTGT
GCCCGTTCATATGGATCCATGGGCTTTGTTTGATATTGATAGGTGGACTATTACTGATTTTGAAAGAAGCATTAATCTTA
GAAGATCTATTACAAGAGCGGTTGAACAGCATATTGTAGCTTTAGATGATGTTGATGCTGTTAGTGTGAATCTTGTTATG
CCCGAGAAAGCTCTTTTTAAAGAGTCTCAAGAGCCTGTTAAGGCATCTGTTAGAATTACCCCAAGACCCGGTTCTGATAT
TATTACCAATAGAAAAAAAGTTGAAGGACTTGTTAAACTTATTCAGTATGCCATTGAAGGTCTTGAATCTGACAATATTG
CTATTGTTGATAATAGTGGAACTATTTTAAATGATTTTTCTAATTTGGATGGGATAGATAGAATAGACTTAGCAGAAAAA
GAGCGTAAGTTAAAGCTTAAGTATGAAGCTATGCTTAGGGGTGAAATCGATTCTGCATTAAGTAAGGTTTTGTCTGTTGA
TAGATTTATGATAGCAAGAGTAAATGTAAAGCTTGATACTTCAAAAGAAACTACAGAGTCTAAAGAGTATGCCCCTATTG
AGCTTCAATCTCAAGATCCAAAAGCTTCTTATAACACTAGGAAAGTAAGCGATTCAACTATTATATCTTCTCAGACTCAA
AAAAAAGAATATCAGGGTCAAGGATATAGTCCCTGGGGACCTCCTGGGCAGGAAGGCAATACCCCTCCTGAATATCAGGA
TTTAAGTGATATTACTGGTAAATATAATGAGTCTCAAGAGATCAAAAATGTTGCTTTGAATGAAAAAAAATCTACAAGCG
AAAAAGAGCCCGCTAGGATTGTAGGTGTTTCTCTTGGTATTTTCGTAGATGGTATTTGGAATTTTGTGTACGATGAGAAG
GGAGATTTCGTAATAGAAAATGGAATGAGAAAAAGAGAATATAAGCCTATGGCATTAGAAGAAATAAAAAATATTGAAGA
TGTTTTGCAAAGTTCTTTTGAATATAAGCCAGAAAGAGGCGATTCAATAACGGTTAGAAATATATCTTTTGATCGTATGA
ATGAATTTAGAGAAATAGATGAAAATTATTTTGCAAGTGAAAGGTTTAAATATTTCTTATTTATTGCAAGTATAGTATTT
TCATTATTAATTTTAGTATTTACGATATTTTTTGCTATTTCTAGAGAACTTGAGAGACGAAGACGTCTTAGAGAAGAGGA
ATTAGCAAAGCAAGCACACTTAAGGCGCCAACAAGCCTTAATGGATGGTGGTGACGATATTGGCGTTGACGATGTTGTTG
GTGGGATTAGAGAAGGCGATGAGCTTCAAAGCAATGCTGAGCTTTTAGCCAGAGAAAAGCCAGAAGATGTTGCTAAGCTT
ATAAGAACATGGCTTTTGAAAAACGCGTAA

Upstream 100 bases:

>100_bases
CTAAGGCGAATATGAATTTAAGCATTTTAAAGGCTGTTGTTGAGAGAGGTGTGAAGGCTTATCAAGATATAATCAATATT
CGTTAAGGAGCCATAAGATT

Downstream 100 bases:

>100_bases
AAGGTAGTAATTGATATGGAAGAAAAAAAAGAAAAGGAGATTCTGGATGTTTCTGCTTTAACAGGCAAGCAAAAGGCCGC
TATTTTGTTGGTTTCAATAG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MSKFFTNFFVSAKGIFKKASTVQKIALGLIIFFVILALVFLIGFSTKSQSIALFGVEIKDQYLLDRISQRLDRENVKYFL
SSDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDIDRWTITDFERSINLRRSITRAVEQHIVALDDVDAVSVNLVM
PEKALFKESQEPVKASVRITPRPGSDIITNRKKVEGLVKLIQYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEK
ERKLKLKYEAMLRGEIDSALSKVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDPKASYNTRKVSDSTIISSQTQ
KKEYQGQGYSPWGPPGQEGNTPPEYQDLSDITGKYNESQEIKNVALNEKKSTSEKEPARIVGVSLGIFVDGIWNFVYDEK
GDFVIENGMRKREYKPMALEEIKNIEDVLQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASERFKYFLFIASIVF
SLLILVFTIFFAISRELERRRRLREEELAKQAHLRRQQALMDGGDDIGVDDVVGGIREGDELQSNAELLAREKPEDVAKL
IRTWLLKNA

Sequences:

>Translated_569_residues
MSKFFTNFFVSAKGIFKKASTVQKIALGLIIFFVILALVFLIGFSTKSQSIALFGVEIKDQYLLDRISQRLDRENVKYFL
SSDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDIDRWTITDFERSINLRRSITRAVEQHIVALDDVDAVSVNLVM
PEKALFKESQEPVKASVRITPRPGSDIITNRKKVEGLVKLIQYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEK
ERKLKLKYEAMLRGEIDSALSKVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDPKASYNTRKVSDSTIISSQTQ
KKEYQGQGYSPWGPPGQEGNTPPEYQDLSDITGKYNESQEIKNVALNEKKSTSEKEPARIVGVSLGIFVDGIWNFVYDEK
GDFVIENGMRKREYKPMALEEIKNIEDVLQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASERFKYFLFIASIVF
SLLILVFTIFFAISRELERRRRLREEELAKQAHLRRQQALMDGGDDIGVDDVVGGIREGDELQSNAELLAREKPEDVAKL
IRTWLLKNA
>Mature_568_residues
SKFFTNFFVSAKGIFKKASTVQKIALGLIIFFVILALVFLIGFSTKSQSIALFGVEIKDQYLLDRISQRLDRENVKYFLS
SDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDIDRWTITDFERSINLRRSITRAVEQHIVALDDVDAVSVNLVMP
EKALFKESQEPVKASVRITPRPGSDIITNRKKVEGLVKLIQYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEKE
RKLKLKYEAMLRGEIDSALSKVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDPKASYNTRKVSDSTIISSQTQK
KEYQGQGYSPWGPPGQEGNTPPEYQDLSDITGKYNESQEIKNVALNEKKSTSEKEPARIVGVSLGIFVDGIWNFVYDEKG
DFVIENGMRKREYKPMALEEIKNIEDVLQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASERFKYFLFIASIVFS
LLILVFTIFFAISRELERRRRLREEELAKQAHLRRQQALMDGGDDIGVDDVVGGIREGDELQSNAELLAREKPEDVAKLI
RTWLLKNA

Specific function: The M ring may be actively involved in energy transduction

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family

Homologues:

Organism=Escherichia coli, GI1788248, Length=448, Percent_Identity=25.8928571428571, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLIF_BORBU (Q44912)

Other databases:

- EMBL:   U43739
- EMBL:   L76303
- EMBL:   L40501
- EMBL:   AE000783
- PIR:   C70136
- RefSeq:   NP_212425.1
- ProteinModelPortal:   Q44912
- EnsemblBacteria:   EBBORT00000008725
- GeneID:   1195128
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0291
- NMPDR:   fig|224326.1.peg.675
- TIGR:   BB_0291
- GeneTree:   EBGT00050000007131
- HOGENOM:   HBG352882
- OMA:   LGPENFT
- PhylomeDB:   Q44912
- ProtClustDB:   PRK06007
- BioCyc:   BBUR224326:BB_0291-MONOMER
- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182
- PRINTS:   PR01009
- TIGRFAMs:   TIGR00206

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C

EC number: NA

Molecular weight: Translated: 65134; Mature: 65003

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc0b676c)-; HASH(0xc883438)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFFTNFFVSAKGIFKKASTVQKIALGLIIFFVILALVFLIGFSTKSQSIALFGVEIKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHH
QYLLDRISQRLDRENVKYFLSSDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDID
HHHHHHHHHHHCHHHHEEEECCCCEEEECHHHHHHHHHHHHHHHHCCCEECCCEEEEECC
RWTITDFERSINLRRSITRAVEQHIVALDDVDAVSVNLVMPEKALFKESQEPVKASVRIT
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCCCCCCEEEEEEC
PRPGSDIITNRKKVEGLVKLIQYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEK
CCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCCCHHHH
ERKLKLKYEAMLRGEIDSALSKVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCEEECCCCC
KASYNTRKVSDSTIISSQTQKKEYQGQGYSPWGPPGQEGNTPPEYQDLSDITGKYNESQE
CCCCCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHH
IKNVALNEKKSTSEKEPARIVGVSLGIFVDGIWNFVYDEKGDFVIENGMRKREYKPMALE
HHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCHHHH
EIKNIEDVLQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASERFKYFLFIASIVF
HHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLILVFTIFFAISRELERRRRLREEELAKQAHLRRQQALMDGGDDIGVDDVVGGIREGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCH
ELQSNAELLAREKPEDVAKLIRTWLLKNA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
SKFFTNFFVSAKGIFKKASTVQKIALGLIIFFVILALVFLIGFSTKSQSIALFGVEIKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHH
QYLLDRISQRLDRENVKYFLSSDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDID
HHHHHHHHHHHCHHHHEEEECCCCEEEECHHHHHHHHHHHHHHHHCCCEECCCEEEEECC
RWTITDFERSINLRRSITRAVEQHIVALDDVDAVSVNLVMPEKALFKESQEPVKASVRIT
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCCCCCCEEEEEEC
PRPGSDIITNRKKVEGLVKLIQYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEK
CCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCCCCCCCCCHHHH
ERKLKLKYEAMLRGEIDSALSKVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCEEECCCCC
KASYNTRKVSDSTIISSQTQKKEYQGQGYSPWGPPGQEGNTPPEYQDLSDITGKYNESQE
CCCCCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHH
IKNVALNEKKSTSEKEPARIVGVSLGIFVDGIWNFVYDEKGDFVIENGMRKREYKPMALE
HHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCHHHH
EIKNIEDVLQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASERFKYFLFIASIVF
HHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLILVFTIFFAISRELERRRRLREEELAKQAHLRRQQALMDGGDDIGVDDVVGGIREGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCH
ELQSNAELLAREKPEDVAKLIRTWLLKNA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685