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The map label for this gene is engD [H]

Identifier: 15594580

GI number: 15594580

Start: 239144

End: 240250

Strand: Reverse

Name: engD [H]

Synonym: BB0235

Alternate gene names: 15594580

Gene position: 240250-239144 (Counterclockwise)

Preceding gene: 15594581

Following gene: 15594579

Centisome position: 26.38

GC content: 31.07

Gene sequence:

>1107_bases
ATGAGACTTAATGCGGGGATTGTGGGACTTCCCAATGTGGGTAAATCTACTTTATTTTCATCTTTGACATCATCTAATGT
TGAGATCGCGAATTATCCTTTTTGTACCATTGAGCCAAATATAGGAATAGTAGAGATTCCCGATGAAAGGCTTTTAAAAA
TCTCAGAGTGCATTATTCCACGCAAAATTATTCCTGCTGTTATGGAATTTGTGGATATTGCAGGTCTTGTCAAAGGAGCA
TCAACAGGCGAAGGGCTTGGCAATAAATTTTTGGCTAATATTCGCGAAGTTTCTCTTATTGTTCATGTTGTAAGATGTTT
TGAAGAAAAAGAGGTTATTCATATTAATGACGATATTAATCCTGAGAAAGATATAACTACAATTAATGTTGAGCTTTGTC
TATCTGATCTTGAAACTGTTCAAAAAAGCCTTCAAAAACAAGAAAAAAATGCCAAAAGCACTGATAAGAAAATTAGTGAA
TCTTCTAAGGCAATTGTTTTAATGTTAAAAAGACTTGAAGACCATTTAAGCAATATGAATCCTGCTGTTGAGTTTAAATT
TGATGATTTTGAATACAACTTTATTAGATCATTAAATCTTTTGACTTTTAAAAAAGTTTTGTATGTTTGCAATGTTGATG
AAAATTCACTTAGCGGCAATAAATATACAGATATTGTAAAAAATATTGCCTTGAAAGAAGGAAATGATTTTTTAATCTTA
TGTGCCAAAATTGAAGCTGAACTTGCCCAAATAAAGGATATATCTTATAAAAATGAAATGTTAAAATTATTTGGAATAAA
TGATAGCGGTCTTGGCAATTTAATTAAAAAAGCCTATTATACTTTGGGCCTTAGAACCTATTTTACAGCAGGTTTGCAAG
AAGTTAGAGCTTGGACATTCAAGCAGGGCATGAGAGCACCCAAAGCTGCTGGAATAATTCATAGTGATTTTGAGAGAGGT
TTTATTAAAGCAGAAGTTTATTCTTGTGAGGATTTATTTAAATTTCAAAGTGTTCAAAAATTAAAAGAGAAAGGACTTGT
TAGAATTGAAGGCAAAGAATATATTGTAAGAGATGGAGATGTAATCTTTTTTAAATTTAATGTGTAG

Upstream 100 bases:

>100_bases
ATTTATCTGTTGAAGCAAATATAAATCAACAGACAGGAAGAGGGGAGTTTGCATATTTTATTAATTAGATTTCTTTTTGA
TTTTATTAGAGGTTTAGGCT

Downstream 100 bases:

>100_bases
ATTTATTTTTATATTATGCGTGATTTATTTTTTAATAATGTAAAATTAATTAGTTTGTTTGTTTTTAATGTTGTGATTTG
TTTGCTTTATTTGGGTTTTT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRLNAGIVGLPNVGKSTLFSSLTSSNVEIANYPFCTIEPNIGIVEIPDERLLKISECIIPRKIIPAVMEFVDIAGLVKGA
STGEGLGNKFLANIREVSLIVHVVRCFEEKEVIHINDDINPEKDITTINVELCLSDLETVQKSLQKQEKNAKSTDKKISE
SSKAIVLMLKRLEDHLSNMNPAVEFKFDDFEYNFIRSLNLLTFKKVLYVCNVDENSLSGNKYTDIVKNIALKEGNDFLIL
CAKIEAELAQIKDISYKNEMLKLFGINDSGLGNLIKKAYYTLGLRTYFTAGLQEVRAWTFKQGMRAPKAAGIIHSDFERG
FIKAEVYSCEDLFKFQSVQKLKEKGLVRIEGKEYIVRDGDVIFFKFNV

Sequences:

>Translated_368_residues
MRLNAGIVGLPNVGKSTLFSSLTSSNVEIANYPFCTIEPNIGIVEIPDERLLKISECIIPRKIIPAVMEFVDIAGLVKGA
STGEGLGNKFLANIREVSLIVHVVRCFEEKEVIHINDDINPEKDITTINVELCLSDLETVQKSLQKQEKNAKSTDKKISE
SSKAIVLMLKRLEDHLSNMNPAVEFKFDDFEYNFIRSLNLLTFKKVLYVCNVDENSLSGNKYTDIVKNIALKEGNDFLIL
CAKIEAELAQIKDISYKNEMLKLFGINDSGLGNLIKKAYYTLGLRTYFTAGLQEVRAWTFKQGMRAPKAAGIIHSDFERG
FIKAEVYSCEDLFKFQSVQKLKEKGLVRIEGKEYIVRDGDVIFFKFNV
>Mature_368_residues
MRLNAGIVGLPNVGKSTLFSSLTSSNVEIANYPFCTIEPNIGIVEIPDERLLKISECIIPRKIIPAVMEFVDIAGLVKGA
STGEGLGNKFLANIREVSLIVHVVRCFEEKEVIHINDDINPEKDITTINVELCLSDLETVQKSLQKQEKNAKSTDKKISE
SSKAIVLMLKRLEDHLSNMNPAVEFKFDDFEYNFIRSLNLLTFKKVLYVCNVDENSLSGNKYTDIVKNIALKEGNDFLIL
CAKIEAELAQIKDISYKNEMLKLFGINDSGLGNLIKKAYYTLGLRTYFTAGLQEVRAWTFKQGMRAPKAAGIIHSDFERG
FIKAEVYSCEDLFKFQSVQKLKEKGLVRIEGKEYIVRDGDVIFFKFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=373, Percent_Identity=39.6782841823056, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI58761502, Length=185, Percent_Identity=37.2972972972973, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI24308117, Length=114, Percent_Identity=31.5789473684211, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787454, Length=368, Percent_Identity=48.3695652173913, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789574, Length=147, Percent_Identity=25.8503401360544, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=373, Percent_Identity=37.5335120643432, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17552324, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=376, Percent_Identity=39.3617021276596, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=35.6955380577428, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321649, Length=258, Percent_Identity=27.906976744186, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24640873, Length=377, Percent_Identity=38.7267904509284, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24640877, Length=377, Percent_Identity=38.7267904509284, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24640875, Length=377, Percent_Identity=38.7267904509284, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24640879, Length=336, Percent_Identity=36.3095238095238, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24585318, Length=104, Percent_Identity=36.5384615384615, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 41489; Mature: 41489

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNAGIVGLPNVGKSTLFSSLTSSNVEIANYPFCTIEPNIGIVEIPDERLLKISECIIP
CCCCCCEEECCCCCHHHHHHHHHCCCEEEECCCEEEECCCCCEEECCHHHHHHHHHHHCC
RKIIPAVMEFVDIAGLVKGASTGEGLGNKFLANIREVSLIVHVVRCFEEKEVIHINDDIN
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PEKDITTINVELCLSDLETVQKSLQKQEKNAKSTDKKISESSKAIVLMLKRLEDHLSNMN
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PAVEFKFDDFEYNFIRSLNLLTFKKVLYVCNVDENSLSGNKYTDIVKNIALKEGNDFLIL
CEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEE
CAKIEAELAQIKDISYKNEMLKLFGINDSGLGNLIKKAYYTLGLRTYFTAGLQEVRAWTF
EEEHHHHHHHHHCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQGMRAPKAAGIIHSDFERGFIKAEVYSCEDLFKFQSVQKLKEKGLVRIEGKEYIVRDGD
HHHCCCCHHCCCHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCEEEECCCEEEEECCC
VIFFKFNV
EEEEEECC
>Mature Secondary Structure
MRLNAGIVGLPNVGKSTLFSSLTSSNVEIANYPFCTIEPNIGIVEIPDERLLKISECIIP
CCCCCCEEECCCCCHHHHHHHHHCCCEEEECCCEEEECCCCCEEECCHHHHHHHHHHHCC
RKIIPAVMEFVDIAGLVKGASTGEGLGNKFLANIREVSLIVHVVRCFEEKEVIHINDDIN
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PEKDITTINVELCLSDLETVQKSLQKQEKNAKSTDKKISESSKAIVLMLKRLEDHLSNMN
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PAVEFKFDDFEYNFIRSLNLLTFKKVLYVCNVDENSLSGNKYTDIVKNIALKEGNDFLIL
CEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEE
CAKIEAELAQIKDISYKNEMLKLFGINDSGLGNLIKKAYYTLGLRTYFTAGLQEVRAWTF
EEEHHHHHHHHHCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQGMRAPKAAGIIHSDFERGFIKAEVYSCEDLFKFQSVQKLKEKGLVRIEGKEYIVRDGD
HHHCCCCHHCCCHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCEEEECCCEEEEECCC
VIFFKFNV
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]