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The map label for this gene is trmE [H]

Identifier: 15594524

GI number: 15594524

Start: 181408

End: 182802

Strand: Reverse

Name: trmE [H]

Synonym: BB0179

Alternate gene names: 15594524

Gene position: 182802-181408 (Counterclockwise)

Preceding gene: 15594530

Following gene: 15594523

Centisome position: 20.07

GC content: 29.46

Gene sequence:

>1395_bases
ATGAGTAAGTTTTTTGAGAGAGATGATGATATTGTAGCTCTTGCAACACCTTTTTTAAGTAGTGCTTTATGTGTGATTCG
TAGCAGTGGTGCTTCTTCTATATCTAAATTTTCTAAAATCTTTTCAAATCATTCAGCTCTCAATTCAGCATCTGGGAATA
CGATTCATTACGGTTATATATTAGATAGTGAGAATGGTTGCAAGGTAGATGAAGTTGTTGTTTGTTTATATCGAGCACCA
AAAAGCTTTACAGGGCAAGATGCTATTGAAGTTATGGCGCATGGTTCTGTGATTGGAATTAAAAAGATTATAGATTTGTT
TTTAAAAAGTGGGTTTAGGATGGCTGAACCTGGTGAATTTACTTTACGTGCATTTCTTGCTAAAAAAATTGATCTTACAA
AGGCAGAGGCAATTCATGAGATTATTTTTGCCAAAACCAATAAAACTTATTCTCTTGCAGTTAATAAACTTTCTGGAGCT
TTGTTTGTTAAAATAGATGCAATAAAAAAAAGTATTTTAAATTTTCTATCAGCTGTTAGTGTTTATCTTGATTATGAGGT
TGATGATCATGAGATTAGTATTCCTTTTGACTTAATTTTAAGTAGCAAGGCTGAACTTAAAAAATTAATTAATTCTTATA
AGGTTTATGAAAAAATTGATAATGGTGTTGCTTTGGTTTTAGCAGGTTCTGTTAATGCTGGAAAGTCGTCGTTATTTAAT
TTATTTCTCAAAAAAGATAGATCAATTGTTTCTTCATATCCAGGCACTACAAGAGATTATATTGAGGCAAGTTTTGAGCT
TGATGGTATTTTATTTAATCTTTTTGATACAGCAGGACTAAGAGATGCTGACAATTTTGTTGAGAGATTGGGAATTGAGA
AAAGTAACTCTTTAATAAAGGAAGCATCTTTAGTGATTTATGTGATCGATGTTAGTTCAAATTTAACAAAAGATGATTTC
TTATTTATTGATTCAAATAAATCTAATAGTAAAATATTGTTTGTTTTAAATAAGATAGATTTAAAGATAAATAAGTCCAC
TGAAGAATTTGTTCGCTCAAAGGTTTTAAATTCTTCAAATTTAATAATGATTAGTACTAAAAATTTAGAAGGAATAGATA
TTCTTTATGACAAAATAAGGGCTTTAATCTCTTATGAGAGAGTCGAGATTGGACTTGATGATATAATAATATCATCAAAT
CGTCAGATGCAACTTTTGGAGAAAGCTTATGCTTTGATTTTAGATTTACTTAGTAAAATTGATCGCCAAGTAAGTTATGA
TATGTTAGCATTTGATGCTTATGAGATTATCAACTGTTTAGGGGAAATAACAGGAGAAGTTAGTAGTGAAGATGTTCTTG
ACAATATGTTTAAGAATTTTTGTTTGGGGAAATAA

Upstream 100 bases:

>100_bases
TCAAGTTTTGTTTTCATTTTTATGAAATTAATTATAAGCTATTATAAACATATTTACAAATCAATTTTGTCTTTTTTATT
GTGTTATAATACATTAAAGT

Downstream 100 bases:

>100_bases
ATATGGATTTTGACGCAATTGTTATTGGAGGGGGGCATGCGGGGATTGAAGCTGCTCTTGCTCTTTCAAGGTTGAATTTT
AAAACTTTAATGATTACTCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSKFFERDDDIVALATPFLSSALCVIRSSGASSISKFSKIFSNHSALNSASGNTIHYGYILDSENGCKVDEVVVCLYRAP
KSFTGQDAIEVMAHGSVIGIKKIIDLFLKSGFRMAEPGEFTLRAFLAKKIDLTKAEAIHEIIFAKTNKTYSLAVNKLSGA
LFVKIDAIKKSILNFLSAVSVYLDYEVDDHEISIPFDLILSSKAELKKLINSYKVYEKIDNGVALVLAGSVNAGKSSLFN
LFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVERLGIEKSNSLIKEASLVIYVIDVSSNLTKDDF
LFIDSNKSNSKILFVLNKIDLKINKSTEEFVRSKVLNSSNLIMISTKNLEGIDILYDKIRALISYERVEIGLDDIIISSN
RQMQLLEKAYALILDLLSKIDRQVSYDMLAFDAYEIINCLGEITGEVSSEDVLDNMFKNFCLGK

Sequences:

>Translated_464_residues
MSKFFERDDDIVALATPFLSSALCVIRSSGASSISKFSKIFSNHSALNSASGNTIHYGYILDSENGCKVDEVVVCLYRAP
KSFTGQDAIEVMAHGSVIGIKKIIDLFLKSGFRMAEPGEFTLRAFLAKKIDLTKAEAIHEIIFAKTNKTYSLAVNKLSGA
LFVKIDAIKKSILNFLSAVSVYLDYEVDDHEISIPFDLILSSKAELKKLINSYKVYEKIDNGVALVLAGSVNAGKSSLFN
LFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVERLGIEKSNSLIKEASLVIYVIDVSSNLTKDDF
LFIDSNKSNSKILFVLNKIDLKINKSTEEFVRSKVLNSSNLIMISTKNLEGIDILYDKIRALISYERVEIGLDDIIISSN
RQMQLLEKAYALILDLLSKIDRQVSYDMLAFDAYEIINCLGEITGEVSSEDVLDNMFKNFCLGK
>Mature_463_residues
SKFFERDDDIVALATPFLSSALCVIRSSGASSISKFSKIFSNHSALNSASGNTIHYGYILDSENGCKVDEVVVCLYRAPK
SFTGQDAIEVMAHGSVIGIKKIIDLFLKSGFRMAEPGEFTLRAFLAKKIDLTKAEAIHEIIFAKTNKTYSLAVNKLSGAL
FVKIDAIKKSILNFLSAVSVYLDYEVDDHEISIPFDLILSSKAELKKLINSYKVYEKIDNGVALVLAGSVNAGKSSLFNL
FLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVERLGIEKSNSLIKEASLVIYVIDVSSNLTKDDFL
FIDSNKSNSKILFVLNKIDLKINKSTEEFVRSKVLNSSNLIMISTKNLEGIDILYDKIRALISYERVEIGLDDIIISSNR
QMQLLEKAYALILDLLSKIDRQVSYDMLAFDAYEIINCLGEITGEVSSEDVLDNMFKNFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=488, Percent_Identity=28.2786885245902, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI193082993, Length=478, Percent_Identity=28.0334728033473, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI193082988, Length=517, Percent_Identity=27.2727272727273, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI2367268, Length=477, Percent_Identity=29.3501048218029, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87082120, Length=171, Percent_Identity=29.2397660818713, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=479, Percent_Identity=27.7661795407098, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323665, Length=505, Percent_Identity=27.7227722772277, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=28.6902286902287, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51606; Mature: 51475

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFFERDDDIVALATPFLSSALCVIRSSGASSISKFSKIFSNHSALNSASGNTIHYGYI
CCCCCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
LDSENGCKVDEVVVCLYRAPKSFTGQDAIEVMAHGSVIGIKKIIDLFLKSGFRMAEPGEF
EECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCHH
TLRAFLAKKIDLTKAEAIHEIIFAKTNKTYSLAVNKLSGALFVKIDAIKKSILNFLSAVS
HHHHHHHHHCCHHHHHHHHHHHHEECCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHH
VYLDYEVDDHEISIPFDLILSSKAELKKLINSYKVYEKIDNGVALVLAGSVNAGKSSLFN
HEEEEEECCCEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
LFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVERLGIEKSNSLIK
HHHHHCHHHHHCCCCCCHHHHHEEEEECCEEEEHHHCCCCCCHHHHHHHHCCCCCCHHHH
EASLVIYVIDVSSNLTKDDFLFIDSNKSNSKILFVLNKIDLKINKSTEEFVRSKVLNSSN
CCCEEEEEEECCCCCCCCCEEEEECCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCCC
LIMISTKNLEGIDILYDKIRALISYERVEIGLDDIIISSNRQMQLLEKAYALILDLLSKI
EEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHEEECCCCHHHHHHHHHHHHHHHHHHH
DRQVSYDMLAFDAYEIINCLGEITGEVSSEDVLDNMFKNFCLGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKFFERDDDIVALATPFLSSALCVIRSSGASSISKFSKIFSNHSALNSASGNTIHYGYI
CCCCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
LDSENGCKVDEVVVCLYRAPKSFTGQDAIEVMAHGSVIGIKKIIDLFLKSGFRMAEPGEF
EECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCHH
TLRAFLAKKIDLTKAEAIHEIIFAKTNKTYSLAVNKLSGALFVKIDAIKKSILNFLSAVS
HHHHHHHHHCCHHHHHHHHHHHHEECCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHH
VYLDYEVDDHEISIPFDLILSSKAELKKLINSYKVYEKIDNGVALVLAGSVNAGKSSLFN
HEEEEEECCCEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
LFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVERLGIEKSNSLIK
HHHHHCHHHHHCCCCCCHHHHHEEEEECCEEEEHHHCCCCCCHHHHHHHHCCCCCCHHHH
EASLVIYVIDVSSNLTKDDFLFIDSNKSNSKILFVLNKIDLKINKSTEEFVRSKVLNSSN
CCCEEEEEEECCCCCCCCCEEEEECCCCCCEEEEEEEEEEEEECCCHHHHHHHHHCCCCC
LIMISTKNLEGIDILYDKIRALISYERVEIGLDDIIISSNRQMQLLEKAYALILDLLSKI
EEEEEECCCCCHHHHHHHHHHHHHHHHHEECHHHEEECCCCHHHHHHHHHHHHHHHHHHH
DRQVSYDMLAFDAYEIINCLGEITGEVSSEDVLDNMFKNFCLGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA