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The map label for this gene is mnmG [H]

Identifier: 15594523

GI number: 15594523

Start: 179540

End: 181423

Strand: Reverse

Name: mnmG [H]

Synonym: BB0178

Alternate gene names: 15594523

Gene position: 181423-179540 (Counterclockwise)

Preceding gene: 15594524

Following gene: 15594522

Centisome position: 19.92

GC content: 33.97

Gene sequence:

>1884_bases
TTGTTTGGGGAAATAAATATGGATTTTGACGCAATTGTTATTGGAGGGGGGCATGCGGGGATTGAAGCTGCTCTTGCTCT
TTCAAGGTTGAATTTTAAAACTTTAATGATTACTCAAAATTTAGATACAATAGGCAAGCTTTCTTGTAATCCTGCTATTG
GTGGGCTTGCTAAGGGTAATATGGTTAGAGAAATTGATGCTCTTGGTGGTGAAATGGGTCGTATTATTGACTTTAGCATG
ATTCAGTTTAGAGTTTTAAACAAAAGTCGTGGCCCAGCAGTTCAAGCTCCACGTGCTCAAGCTGATAAATTAATGTACCA
AACCAAGGCTAAAGAAACTTTAGAACGTCAAGACAATCTTGATCTTTTTCAGGATACAGTTGTGGATTTTCTTCTTAATT
CAATGAGAAATGAAATTGAAGGTGTTGTTACAGAGAGAGGCAATAAGTTTAGATCAAGCGTTGTGGTACTTACAACAGGG
ACGTTTCTTCGAGGTAAAATATTTATTGGCGAGTATAGAGCTGATATGGGTAGGCTTGCTGAATTTTCTGCTTATGGGCT
TGATAAAACTTTACTTGGTCTTGGATTTGAAATGGGTAGGCTTAAAACGGGCACTCCAGCAAGAATTCATAAAAAAAGTG
TGGATTTTTCAAAGACGGAGGTTCAATTTGGAGATTCAGATATTATTCCTTTCTCTTTTTCAAATGGCAAGTTAGATAAA
TCTCAACTTTCATGTTATGTAACCTATACTAATAAAAAAACTCACGAAATAATTAGCGAGAATATGCACCTATCACCTCT
TTATTCTGGTGAGATTGTGGGTAATGGTCCAAGATATTGTCCTTCTATTGAGGATAAAATAGTAAAATTTAAAGATAAAG
ATAGGCATCAAATTTTTATTGAGCCTGAAGGTTTTAATACTGAAGAAATGTATCTTAATGGTCTTAGCTCTTCTTTGCCT
GAAAATATTCAGCAAAAATTGATTAACAGCATTGAAGGCCTTGAGCATGCTGTTATTACAAGGCCCGGTTATGCTGTTGA
GTATGATTATATAAATCCAATTGAACTTTACCCAAATCTTGAGAGCAAAAGAGTCAAAGGGCTTTTTATAGCGGGGCAGA
CTAATGGCTCTTCAGGGTATGAAGAAGCAGCAGCTCAAGGGTTAATGGCTGGAATTAATGCTGCTCTAAGACTTCAAAAT
AAAAAGCCAATGATTTTAACAAGAACTAGCTCTTATATTGGAGTTCTTATTGACGATCTTGTTACCAAAGGCACTAAAGA
ACCTTACAGAATGTTTACCTCTAGAGCTGAGCACAGACTTAATTTAAGGCATGATACTAGTGACAAGCGTTTGATTAAGA
TTGGATATGATCTTGGGCTTGTTGATGAGAAGAGGTATTCAAGATATCTTTTTAAAGAGAGCAGGGTTGAAGAGATAAAG
GAGCTTTTAAAGCAAAGGCGCCTTAGTTTAAAAGATGTTGTTGATGAACAATTAAAAAAACATGTTAGTAAAGATTTTTA
CCATATTTTAAAAGATCCTTCTATTAGTTTAGATAATCTTATAAAGATTGATCCAAGTTTAAGTGATTCAAAAGTAATTT
TAGAGCAAGTTGAATTAGATGTTAAATATGAAGGCTATATTAATAGACAAAAGGATTTAATTAAAAGGCTTGATAATCTA
GAGCTTGTCAAGCTACCATTTGATTTTAATTATGAAATTATTGAAGGCCTTTCAAGAGAAGCTAGAGAAAAATTTTCTAA
GATTCAACCAGCTACCCTTGCTCAAGCAAGTCGAATTCCTGGAATAAGAAGTACAGATATTACTGTTTTGTTAATATATT
TTTCAAATCCTAAAAATAAGGTAGTTATAAATTTTTCTTTATGA

Upstream 100 bases:

>100_bases
ATATGTTAGCATTTGATGCTTATGAGATTATCAACTGTTTAGGGGAAATAACAGGAGAAGTTAGTAGTGAAGATGTTCTT
GACAATATGTTTAAGAATTT

Downstream 100 bases:

>100_bases
TAAGTGATATTGAATTTGCTTTGTCAGAACATAATTTTCAGTTTGCTTACAAAGATCTTCAGAAGATAAATTTATATATA
AAGAGAATTTTACTTTTAAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MFGEINMDFDAIVIGGGHAGIEAALALSRLNFKTLMITQNLDTIGKLSCNPAIGGLAKGNMVREIDALGGEMGRIIDFSM
IQFRVLNKSRGPAVQAPRAQADKLMYQTKAKETLERQDNLDLFQDTVVDFLLNSMRNEIEGVVTERGNKFRSSVVVLTTG
TFLRGKIFIGEYRADMGRLAEFSAYGLDKTLLGLGFEMGRLKTGTPARIHKKSVDFSKTEVQFGDSDIIPFSFSNGKLDK
SQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDRHQIFIEPEGFNTEEMYLNGLSSSLP
ENIQQKLINSIEGLEHAVITRPGYAVEYDYINPIELYPNLESKRVKGLFIAGQTNGSSGYEEAAAQGLMAGINAALRLQN
KKPMILTRTSSYIGVLIDDLVTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKIGYDLGLVDEKRYSRYLFKESRVEEIK
ELLKQRRLSLKDVVDEQLKKHVSKDFYHILKDPSISLDNLIKIDPSLSDSKVILEQVELDVKYEGYINRQKDLIKRLDNL
ELVKLPFDFNYEIIEGLSREAREKFSKIQPATLAQASRIPGIRSTDITVLLIYFSNPKNKVVINFSL

Sequences:

>Translated_627_residues
MFGEINMDFDAIVIGGGHAGIEAALALSRLNFKTLMITQNLDTIGKLSCNPAIGGLAKGNMVREIDALGGEMGRIIDFSM
IQFRVLNKSRGPAVQAPRAQADKLMYQTKAKETLERQDNLDLFQDTVVDFLLNSMRNEIEGVVTERGNKFRSSVVVLTTG
TFLRGKIFIGEYRADMGRLAEFSAYGLDKTLLGLGFEMGRLKTGTPARIHKKSVDFSKTEVQFGDSDIIPFSFSNGKLDK
SQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDRHQIFIEPEGFNTEEMYLNGLSSSLP
ENIQQKLINSIEGLEHAVITRPGYAVEYDYINPIELYPNLESKRVKGLFIAGQTNGSSGYEEAAAQGLMAGINAALRLQN
KKPMILTRTSSYIGVLIDDLVTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKIGYDLGLVDEKRYSRYLFKESRVEEIK
ELLKQRRLSLKDVVDEQLKKHVSKDFYHILKDPSISLDNLIKIDPSLSDSKVILEQVELDVKYEGYINRQKDLIKRLDNL
ELVKLPFDFNYEIIEGLSREAREKFSKIQPATLAQASRIPGIRSTDITVLLIYFSNPKNKVVINFSL
>Mature_627_residues
MFGEINMDFDAIVIGGGHAGIEAALALSRLNFKTLMITQNLDTIGKLSCNPAIGGLAKGNMVREIDALGGEMGRIIDFSM
IQFRVLNKSRGPAVQAPRAQADKLMYQTKAKETLERQDNLDLFQDTVVDFLLNSMRNEIEGVVTERGNKFRSSVVVLTTG
TFLRGKIFIGEYRADMGRLAEFSAYGLDKTLLGLGFEMGRLKTGTPARIHKKSVDFSKTEVQFGDSDIIPFSFSNGKLDK
SQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDRHQIFIEPEGFNTEEMYLNGLSSSLP
ENIQQKLINSIEGLEHAVITRPGYAVEYDYINPIELYPNLESKRVKGLFIAGQTNGSSGYEEAAAQGLMAGINAALRLQN
KKPMILTRTSSYIGVLIDDLVTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKIGYDLGLVDEKRYSRYLFKESRVEEIK
ELLKQRRLSLKDVVDEQLKKHVSKDFYHILKDPSISLDNLIKIDPSLSDSKVILEQVELDVKYEGYINRQKDLIKRLDNL
ELVKLPFDFNYEIIEGLSREAREKFSKIQPATLAQASRIPGIRSTDITVLLIYFSNPKNKVVINFSL

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=634, Percent_Identity=42.4290220820189, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI19882217, Length=659, Percent_Identity=40.8194233687405, Blast_Score=465, Evalue=1e-131,
Organism=Homo sapiens, GI183227703, Length=674, Percent_Identity=39.9109792284866, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI2367273, Length=616, Percent_Identity=47.5649350649351, Blast_Score=585, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17534255, Length=626, Percent_Identity=39.6166134185303, Blast_Score=437, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321202, Length=616, Percent_Identity=41.8831168831169, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24658174, Length=625, Percent_Identity=43.36, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70822; Mature: 70822

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGEINMDFDAIVIGGGHAGIEAALALSRLNFKTLMITQNLDTIGKLSCNPAIGGLAKGN
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCCCCCCCCCC
MVREIDALGGEMGRIIDFSMIQFRVLNKSRGPAVQAPRAQADKLMYQTKAKETLERQDNL
HHHHHHHCCCCCCHHEEHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DLFQDTVVDFLLNSMRNEIEGVVTERGNKFRSSVVVLTTGTFLRGKIFIGEYRADMGRLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEEEEECCEEEEEEEEEHHHHHHHHHH
EFSAYGLDKTLLGLGFEMGRLKTGTPARIHKKSVDFSKTEVQFGDSDIIPFSFSNGKLDK
HHHHCCCCHHHHHCCCHHCCCCCCCCCHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCH
SQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDRHQIFI
HHEEEEEEECCCHHHHHHHCCCEECCCCCCCEECCCCCCCCCHHHHHEECCCCCCCEEEE
EPEGFNTEEMYLNGLSSSLPENIQQKLINSIEGLEHAVITRPGYAVEYDYINPIELYPNL
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCEECCCC
ESKRVKGLFIAGQTNGSSGYEEAAAQGLMAGINAALRLQNKKPMILTRTSSYIGVLIDDL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHEEECCCCCEEEEECCCHHHHHHHHH
VTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKIGYDLGLVDEKRYSRYLFKESRVEEIK
HHCCCCCHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ELLKQRRLSLKDVVDEQLKKHVSKDFYHILKDPSISLDNLIKIDPSLSDSKVILEQVELD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCC
VKYEGYINRQKDLIKRLDNLELVKLPFDFNYEIIEGLSREAREKFSKIQPATLAQASRIP
EEECCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
GIRSTDITVLLIYFSNPKNKVVINFSL
CCCCCCEEEEEEEEECCCCEEEEEEEC
>Mature Secondary Structure
MFGEINMDFDAIVIGGGHAGIEAALALSRLNFKTLMITQNLDTIGKLSCNPAIGGLAKGN
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCHHCCCCCCCCCCCCCCC
MVREIDALGGEMGRIIDFSMIQFRVLNKSRGPAVQAPRAQADKLMYQTKAKETLERQDNL
HHHHHHHCCCCCCHHEEHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DLFQDTVVDFLLNSMRNEIEGVVTERGNKFRSSVVVLTTGTFLRGKIFIGEYRADMGRLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEEEEECCEEEEEEEEEHHHHHHHHHH
EFSAYGLDKTLLGLGFEMGRLKTGTPARIHKKSVDFSKTEVQFGDSDIIPFSFSNGKLDK
HHHHCCCCHHHHHCCCHHCCCCCCCCCHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCH
SQLSCYVTYTNKKTHEIISENMHLSPLYSGEIVGNGPRYCPSIEDKIVKFKDKDRHQIFI
HHEEEEEEECCCHHHHHHHCCCEECCCCCCCEECCCCCCCCCHHHHHEECCCCCCCEEEE
EPEGFNTEEMYLNGLSSSLPENIQQKLINSIEGLEHAVITRPGYAVEYDYINPIELYPNL
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCEECCCC
ESKRVKGLFIAGQTNGSSGYEEAAAQGLMAGINAALRLQNKKPMILTRTSSYIGVLIDDL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHEEECCCCCEEEEECCCHHHHHHHHH
VTKGTKEPYRMFTSRAEHRLNLRHDTSDKRLIKIGYDLGLVDEKRYSRYLFKESRVEEIK
HHCCCCCHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ELLKQRRLSLKDVVDEQLKKHVSKDFYHILKDPSISLDNLIKIDPSLSDSKVILEQVELD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCC
VKYEGYINRQKDLIKRLDNLELVKLPFDFNYEIIEGLSREAREKFSKIQPATLAQASRIP
EEECCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
GIRSTDITVLLIYFSNPKNKVVINFSL
CCCCCCEEEEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA