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The map label for this gene is opuAB [H]

Identifier: 15594490

GI number: 15594490

Start: 145471

End: 146370

Strand: Reverse

Name: opuAB [H]

Synonym: BB0145

Alternate gene names: 15594490

Gene position: 146370-145471 (Counterclockwise)

Preceding gene: 15594491

Following gene: 15594489

Centisome position: 16.07

GC content: 28.0

Gene sequence:

>900_bases
ATGACTAAAGATTTTTTTATATTAAAAATAGATAATTTTTTTGATTTTTTGGTTGATAATTTTTCAATTTCTGATGGAGT
AGGTTTTTCTAAAAGTATAATTTTTTTATATGAAAATTTAAAAAATTTATTTCTTTTTGTTAACCCACTTCTTTTTATTT
TGACTGTATGTTTGCTAAGTTTTGTTTTTTTAAAAAAGAGGTTAATATTTTTAATTTTACCCGGATTCTTTTTTATTTTG
TATTTTAATCTTTGGGAAGCTTCAATGGATACAATAGCTATTATATTTGTTTCTGTATTAGTTTCTGTTATTTTGGGAAT
TCCTATAGGTATTTTGGGCGGATATTTCCCAAGATTTTATGTTTTTTTGAAACCTATTCTAGATTTAATGCAAGCCATGC
CCCCTTTTATTTACTTGATTCCCGCTATTCCTTTTTTTGGAATGGGTACAGCTTCTGCTATTTTTGCTACAATAGTTTTT
GCTATGCCCCCTGTTATTAGATATACAAGGCTGGGAATTGTTCAAGTTTCAGATGAGGTAATAGAGGCAGCTAAGTCCTT
TGGTAGTAGTAATTTGCGCATTCTTTTTCAAGTTCAACTTCCCCTTTCTCTTCAAAGTATAATTGAAGGTATTAATCAGT
CAATAATGATGGCTATATCTATGATAGTAATTGCTGCAATGGTTGGATCGTCTGGTTTGGGCAGAACTGTGATTTACTCT
ATAGAAAGATTAAATTTTGGTGAGGGTTTAATATCTGGATTAGCAGTTGTTATTATAGCTATTATTTTAGATAGGATTAT
GCAATCTATCTTTATTAAATTTAGCTATTTAAACACAGATCATTATGGTGGAAAGAAAGAGAATAAATTTAAAAGATTCT
TAGAAATCTATAATAAATAA

Upstream 100 bases:

>100_bases
TTTAAATAAGCAAGATTATTTAATAATAAAAGAAAAGGGAACTGTTGTCGGATATATTAGTTTAAATGAAATCTCTTATT
TATTATCAAGATAGGAATTT

Downstream 100 bases:

>100_bases
GGAGGATATTTTATGTATAAATTATTTTTATTTTTTATTATTTTTATGTTTTTGTCTTGTGATGAAAAAAAGAGTTCAAA
GAATTTAAAATCGGTAAAAA

Product: glycine/betaine ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTKDFFILKIDNFFDFLVDNFSISDGVGFSKSIIFLYENLKNLFLFVNPLLFILTVCLLSFVFLKKRLIFLILPGFFFIL
YFNLWEASMDTIAIIFVSVLVSVILGIPIGILGGYFPRFYVFLKPILDLMQAMPPFIYLIPAIPFFGMGTASAIFATIVF
AMPPVIRYTRLGIVQVSDEVIEAAKSFGSSNLRILFQVQLPLSLQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
IERLNFGEGLISGLAVVIIAIILDRIMQSIFIKFSYLNTDHYGGKKENKFKRFLEIYNK

Sequences:

>Translated_299_residues
MTKDFFILKIDNFFDFLVDNFSISDGVGFSKSIIFLYENLKNLFLFVNPLLFILTVCLLSFVFLKKRLIFLILPGFFFIL
YFNLWEASMDTIAIIFVSVLVSVILGIPIGILGGYFPRFYVFLKPILDLMQAMPPFIYLIPAIPFFGMGTASAIFATIVF
AMPPVIRYTRLGIVQVSDEVIEAAKSFGSSNLRILFQVQLPLSLQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
IERLNFGEGLISGLAVVIIAIILDRIMQSIFIKFSYLNTDHYGGKKENKFKRFLEIYNK
>Mature_298_residues
TKDFFILKIDNFFDFLVDNFSISDGVGFSKSIIFLYENLKNLFLFVNPLLFILTVCLLSFVFLKKRLIFLILPGFFFILY
FNLWEASMDTIAIIFVSVLVSVILGIPIGILGGYFPRFYVFLKPILDLMQAMPPFIYLIPAIPFFGMGTASAIFATIVFA
MPPVIRYTRLGIVQVSDEVIEAAKSFGSSNLRILFQVQLPLSLQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYSI
ERLNFGEGLISGLAVVIIAIILDRIMQSIFIKFSYLNTDHYGGKKENKFKRFLEIYNK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=186, Percent_Identity=44.0860215053763, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1788449, Length=184, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788451, Length=197, Percent_Identity=24.3654822335025, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786564, Length=153, Percent_Identity=30.718954248366, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33735; Mature: 33604

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDFFILKIDNFFDFLVDNFSISDGVGFSKSIIFLYENLKNLFLFVNPLLFILTVCLLS
CCCCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVFLKKRLIFLILPGFFFILYFNLWEASMDTIAIIFVSVLVSVILGIPIGILGGYFPRFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFLKPILDLMQAMPPFIYLIPAIPFFGMGTASAIFATIVFAMPPVIRYTRLGIVQVSDEV
HHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IEAAKSFGSSNLRILFQVQLPLSLQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
HHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IERLNFGEGLISGLAVVIIAIILDRIMQSIFIKFSYLNTDHYGGKKENKFKRFLEIYNK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKDFFILKIDNFFDFLVDNFSISDGVGFSKSIIFLYENLKNLFLFVNPLLFILTVCLLS
CCCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVFLKKRLIFLILPGFFFILYFNLWEASMDTIAIIFVSVLVSVILGIPIGILGGYFPRFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFLKPILDLMQAMPPFIYLIPAIPFFGMGTASAIFATIVFAMPPVIRYTRLGIVQVSDEV
HHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IEAAKSFGSSNLRILFQVQLPLSLQSIIEGINQSIMMAISMIVIAAMVGSSGLGRTVIYS
HHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IERLNFGEGLISGLAVVIIAIILDRIMQSIFIKFSYLNTDHYGGKKENKFKRFLEIYNK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]