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The map label for this gene is yciL [C]

Identifier: 15594474

GI number: 15594474

Start: 124798

End: 125547

Strand: Reverse

Name: yciL [C]

Synonym: BB0129

Alternate gene names: 15594474

Gene position: 125547-124798 (Counterclockwise)

Preceding gene: 15594475

Following gene: 15594473

Centisome position: 13.79

GC content: 26.8

Gene sequence:

>750_bases
ATGATTGCACTTAAAGCCCCTAGAGTTCACGTTTTTTTAGCTGAAAAGGGAGTGGGGTCAAGAAGATTTTGCGAGGAACT
GATAAGAAAGAAACTTGTAAGAGTTAATAACACTATTGCTAAGCTTGGGGATAAAGTAACTTTAGGAGACAGAATAATTT
ATAAAAAACAGATTTTTGTTTTTAAAGATTTTCAAATTAATAATAGAATTTATCTAGCTCTTAACAAGCCTAGAAATTAT
TTATGTTCTAATTTTGATGTTGATGGAAGAAAGTTAGCAATATCTTTGGTTCAGCCCTTATTTAAAGAGCGTGTGTTTTC
AATTGGTAGGCTTGATTTTAAAAGCTCTGGACTTTTATTATTCACCAATGATGGTAAATTTGCAAACGATATTATTCATC
CAAGGCAAAAAGTTGAAAGAGAATATATTATTGAATCAAAAAAAGATATTGATGAAAATTTGCTTATTTCTTTTAAATCG
GGTATAAAGGTAAAAAAAGAATTTTTTAAATTAAAATCTTATGAAATTTTAAATAAAAATTCTGCTAGATTGATTTTAGA
TGAAGGGAAAAATAGAGAAATAAGAAAAGTGTTTTTGAGCAAGAATATTTTTTTAAAAAAAATTCATAGAATTAGAATTG
GCAATATTAATTTAGATAGTTTAAAGGAGGGTCAAGTAAAAATTGTTCCTTTGTCTAAGATAAATAGTTTAAAATCCAGG
CTGGAGAAATTAAATGATAATAGCAATTGA

Upstream 100 bases:

>100_bases
TTTTCTTATTTTTTAGTATCTCTTGAGTGTAAAATGCTTAATATTGTAAATTTAGGATATTTGAATAATAAATTAGTATT
GTATCGAGGTTGTAAAATTA

Downstream 100 bases:

>100_bases
TGGACCTTCAGCATCAGGAAAAAGTTCAATTGCAAGAGAGCTTGGCGTAAGGCTGAACTATAAATTTATCAGCTCTGGGC
ATCTTTACAGGATAATAACT

Product: hypothetical protein

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIALKAPRVHVFLAEKGVGSRRFCEELIRKKLVRVNNTIAKLGDKVTLGDRIIYKKQIFVFKDFQINNRIYLALNKPRNY
LCSNFDVDGRKLAISLVQPLFKERVFSIGRLDFKSSGLLLFTNDGKFANDIIHPRQKVEREYIIESKKDIDENLLISFKS
GIKVKKEFFKLKSYEILNKNSARLILDEGKNREIRKVFLSKNIFLKKIHRIRIGNINLDSLKEGQVKIVPLSKINSLKSR
LEKLNDNSN

Sequences:

>Translated_249_residues
MIALKAPRVHVFLAEKGVGSRRFCEELIRKKLVRVNNTIAKLGDKVTLGDRIIYKKQIFVFKDFQINNRIYLALNKPRNY
LCSNFDVDGRKLAISLVQPLFKERVFSIGRLDFKSSGLLLFTNDGKFANDIIHPRQKVEREYIIESKKDIDENLLISFKS
GIKVKKEFFKLKSYEILNKNSARLILDEGKNREIRKVFLSKNIFLKKIHRIRIGNINLDSLKEGQVKIVPLSKINSLKSR
LEKLNDNSN
>Mature_249_residues
MIALKAPRVHVFLAEKGVGSRRFCEELIRKKLVRVNNTIAKLGDKVTLGDRIIYKKQIFVFKDFQINNRIYLALNKPRNY
LCSNFDVDGRKLAISLVQPLFKERVFSIGRLDFKSSGLLLFTNDGKFANDIIHPRQKVEREYIIESKKDIDENLLISFKS
GIKVKKEFFKLKSYEILNKNSARLILDEGKNREIRKVFLSKNIFLKKIHRIRIGNINLDSLKEGQVKIVPLSKINSLKSR
LEKLNDNSN

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=31.8367346938775, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788510, Length=170, Percent_Identity=28.8235294117647, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790453, Length=227, Percent_Identity=28.1938325991189, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y129_BORBU (O51155)

Other databases:

- EMBL:   AE000783
- PIR:   A70116
- RefSeq:   NP_212263.1
- ProteinModelPortal:   O51155
- SMR:   O51155
- EnsemblBacteria:   EBBORT00000009115
- GeneID:   1194964
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0129
- NMPDR:   fig|224326.1.peg.513
- TIGR:   BB_0129
- GeneTree:   EBGT00050000007420
- HOGENOM:   HBG754478
- OMA:   ERVFSIG
- ProtClustDB:   CLSK507374
- BioCyc:   BBUR224326:BB_0129-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 28950; Mature: 28950

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALKAPRVHVFLAEKGVGSRRFCEELIRKKLVRVNNTIAKLGDKVTLGDRIIYKKQIFV
CCEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEEEEEE
FKDFQINNRIYLALNKPRNYLCSNFDVDGRKLAISLVQPLFKERVFSIGRLDFKSSGLLL
EEEEEECCEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEE
FTNDGKFANDIIHPRQKVEREYIIESKKDIDENLLISFKSGIKVKKEFFKLKSYEILNKN
EECCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCC
SARLILDEGKNREIRKVFLSKNIFLKKIHRIRIGNINLDSLKEGQVKIVPLSKINSLKSR
CCEEEEECCCCCHHHHHHHHCCHHHHHHHEEEECCCCCCCCCCCCEEEEEHHHHHHHHHH
LEKLNDNSN
HHHHCCCCC
>Mature Secondary Structure
MIALKAPRVHVFLAEKGVGSRRFCEELIRKKLVRVNNTIAKLGDKVTLGDRIIYKKQIFV
CCEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEEEEEE
FKDFQINNRIYLALNKPRNYLCSNFDVDGRKLAISLVQPLFKERVFSIGRLDFKSSGLLL
EEEEEECCEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEE
FTNDGKFANDIIHPRQKVEREYIIESKKDIDENLLISFKSGIKVKKEFFKLKSYEILNKN
EECCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCC
SARLILDEGKNREIRKVFLSKNIFLKKIHRIRIGNINLDSLKEGQVKIVPLSKINSLKSR
CCEEEEECCCCCHHHHHHHHCCHHHHHHHEEEECCCCCCCCCCCCEEEEEHHHHHHHHHH
LEKLNDNSN
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685