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The map label for this gene is tsf [H]

Identifier: 15594467

GI number: 15594467

Start: 118964

End: 119803

Strand: Reverse

Name: tsf [H]

Synonym: BB0122

Alternate gene names: 15594467

Gene position: 119803-118964 (Counterclockwise)

Preceding gene: 15594468

Following gene: 15594466

Centisome position: 13.15

GC content: 31.07

Gene sequence:

>840_bases
ATGAGCATTATTAGTCCTCAGGATGTAAAAAAGCTTCGAGAAGAAACCAATGCTGGTTTTGGAGATTGTAAAAAAGCTTT
GTCTGTTGCTGGTGGAGACTTTGAATTAGCTAAAAAAAAACTTAGAGAAATGGGAATCGCATCTGCTGAGAAAAGACTTG
ACAGAGATGCAAAAGAAGGTAGAGTATTCTCATATTCAAACAATATTTATGCTGGGCTTTTGCTTGTTTCTTGTGAGACG
GACTTTGTTGCTTTGAATCACAATTTTGTTAATTTTGGTAATTCCTTGATCAAGGAATTGGTAGAAAGTGGAATAGATTC
TTTAACTACTTCTCAAGAGTTAGAGCTTAAAAATTTAGCAGCTACAATAAAAGAAAATATTCAGGTTAAAAAAATTTTTA
TTACTAAAATTCAATCTAATGAGTTTGTAAAAATTTATTTGCATGGTGAACAATCCAAGATAGGTGTTTTGGTTAAATTA
AAAGTAAATGATTTTTCTAAAACGGAAGATAAAATTTTTAAAAATTTTGCTATGGATTTAGCTTTGCACGTGGCTGCGTT
TGCTCCTGTTTATTTGAGAAATGATGATGTTTGTCCAAATTATATTAAAGAGCAAGAAGAAATATTTACCAAACAATTAG
AATCTAGTGGTAAGCCGGAAAGCATAATCAAGGGTATAGTTGCGGGAAAAATCAAAAAACATCTTGCTGAGATTTCTCTT
CTTGAGCAAAGTTTTGTAAAGAATGATAAAATCACTGTAAAGGAAATGCTTGAAGAAATTTCAAAAGCAATTTCAAGCAA
GGTAGAAATGGTTGAGTTTAAATATTTAAGAATAGGGTAG

Upstream 100 bases:

>100_bases
TATTCAAATAATTGAAAATTTGAATGAAGAAGATTTGATGAAAGAAATTGAAATTAAAAACGATAAAAGTGATTCTATTG
AAGAAAGGGGAGAGTAATTT

Downstream 100 bases:

>100_bases
ATAATGTGCTCTTTATCAGCAAGGAGGAGTTTATATGGAAGATTATAAGGCTTTTCTAGATGAAAAGATGAACAAGGTTC
TTTTATCACTTGATAATGAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSIISPQDVKKLREETNAGFGDCKKALSVAGGDFELAKKKLREMGIASAEKRLDRDAKEGRVFSYSNNIYAGLLLVSCET
DFVALNHNFVNFGNSLIKELVESGIDSLTTSQELELKNLAATIKENIQVKKIFITKIQSNEFVKIYLHGEQSKIGVLVKL
KVNDFSKTEDKIFKNFAMDLALHVAAFAPVYLRNDDVCPNYIKEQEEIFTKQLESSGKPESIIKGIVAGKIKKHLAEISL
LEQSFVKNDKITVKEMLEEISKAISSKVEMVEFKYLRIG

Sequences:

>Translated_279_residues
MSIISPQDVKKLREETNAGFGDCKKALSVAGGDFELAKKKLREMGIASAEKRLDRDAKEGRVFSYSNNIYAGLLLVSCET
DFVALNHNFVNFGNSLIKELVESGIDSLTTSQELELKNLAATIKENIQVKKIFITKIQSNEFVKIYLHGEQSKIGVLVKL
KVNDFSKTEDKIFKNFAMDLALHVAAFAPVYLRNDDVCPNYIKEQEEIFTKQLESSGKPESIIKGIVAGKIKKHLAEISL
LEQSFVKNDKITVKEMLEEISKAISSKVEMVEFKYLRIG
>Mature_278_residues
SIISPQDVKKLREETNAGFGDCKKALSVAGGDFELAKKKLREMGIASAEKRLDRDAKEGRVFSYSNNIYAGLLLVSCETD
FVALNHNFVNFGNSLIKELVESGIDSLTTSQELELKNLAATIKENIQVKKIFITKIQSNEFVKIYLHGEQSKIGVLVKLK
VNDFSKTEDKIFKNFAMDLALHVAAFAPVYLRNDDVCPNYIKEQEEIFTKQLESSGKPESIIKGIVAGKIKKHLAEISLL
EQSFVKNDKITVKEMLEEISKAISSKVEMVEFKYLRIG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Escherichia coli, GI1786366, Length=262, Percent_Identity=33.587786259542, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 31305; Mature: 31173

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIISPQDVKKLREETNAGFGDCKKALSVAGGDFELAKKKLREMGIASAEKRLDRDAKEG
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCHHCC
RVFSYSNNIYAGLLLVSCETDFVALNHNFVNFGNSLIKELVESGIDSLTTSQELELKNLA
EEEEECCCEEEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ATIKENIQVKKIFITKIQSNEFVKIYLHGEQSKIGVLVKLKVNDFSKTEDKIFKNFAMDL
HHHHHCCEEEEEEEEEECCCCEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
ALHVAAFAPVYLRNDDVCPNYIKEQEEIFTKQLESSGKPESIIKGIVAGKIKKHLAEISL
HHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LEQSFVKNDKITVKEMLEEISKAISSKVEMVEFKYLRIG
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure 
SIISPQDVKKLREETNAGFGDCKKALSVAGGDFELAKKKLREMGIASAEKRLDRDAKEG
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCHHCC
RVFSYSNNIYAGLLLVSCETDFVALNHNFVNFGNSLIKELVESGIDSLTTSQELELKNLA
EEEEECCCEEEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ATIKENIQVKKIFITKIQSNEFVKIYLHGEQSKIGVLVKLKVNDFSKTEDKIFKNFAMDL
HHHHHCCEEEEEEEEEECCCCEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
ALHVAAFAPVYLRNDDVCPNYIKEQEEIFTKQLESSGKPESIIKGIVAGKIKKHLAEISL
HHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LEQSFVKNDKITVKEMLEEISKAISSKVEMVEFKYLRIG
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA