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Identifier: 15594451

GI number: 15594451

Start: 103016

End: 103771

Strand: Reverse

Name: map

Synonym: BB0105

Alternate gene names: 15594451

Gene position: 103771-103016 (Counterclockwise)

Preceding gene: 15594452

Following gene: 15594450

Centisome position: 11.39

GC content: 33.86

Gene sequence:

>756_bases
TTGACTAAACTAAGATTGAAATCTAAAGATGAGATTAAAAAAATAAAAGCTTCAGCAAGTTTGTTAGCGCTTACTTTGTT
AGAAGTTGAGAGAAATATTGTTCCTGGTATTAGCACCAAGGAGCTTGATTTGATAGCCTATGATTTTATTATTAAAAATA
GAGCTAAGCCTGCTTTTAAGGGATATCGTGGGTTTAAGGGCACTATTTGCGCATCTGTTAATGAAGAGGTTATTCACGGC
ATTCCCGGAAAAAGAAAATTAGCGGATGGGGATATTGTTAGTATTGACTGTGGGGTTATTCTTGATGGGTTTTATAGCGA
TATGGCAAAAACTTTTAAGGTTGGAAATGTAGATTCTAGTATCGATAAACTTTTAGAAGTTACAAATGCCTCTCTTTACA
AGGGTATCGCTGAGATGAAAGTTGGAAATAGAATTTTAAATATATCAAAGGCAATAGAAGATTATATAAAGCCGTTTGGT
TTTGGAATAGTTAGAGAATATACAGGCCATGGTGTTGGATTTGAACTTCACGAAGAACCAAGTGTTCCAAATTATTATGC
TCCTTTTTTTAAAAATATTAGAATTCAAGAGGGTATGGTTTTGGCTATTGAGCCTATGGTAAACTTAAGGGGGCACAAGG
TTTCAATAAAAAGTGATGGTTGGACTGTTTTTGCATCTGATCTTAGTTATTCTGCACATTTTGAACATACTGTTGCTGTT
GTTGATGGATTGCCTTTAATTTTAAGTGAAGTTTAA

Upstream 100 bases:

>100_bases
GGCAAAGAGTCTATTTTGGCTTCTTATTCTTTTTACGAAACCGATGAAGGAAGGCTTTATAAAAATATGATAGATACACT
TAGAGAAAGGAATTTGAATT

Downstream 100 bases:

>100_bases
ATTTTAAATTAATCAAATATTTAAATATTATTTATAAAATTGAGTAATTATGTGGAGGTTATAAAAGTGGAAAAAAAGTT
TTTTTCTGGATTTCTTCTTA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTKLRLKSKDEIKKIKASASLLALTLLEVERNIVPGISTKELDLIAYDFIIKNRAKPAFKGYRGFKGTICASVNEEVIHG
IPGKRKLADGDIVSIDCGVILDGFYSDMAKTFKVGNVDSSIDKLLEVTNASLYKGIAEMKVGNRILNISKAIEDYIKPFG
FGIVREYTGHGVGFELHEEPSVPNYYAPFFKNIRIQEGMVLAIEPMVNLRGHKVSIKSDGWTVFASDLSYSAHFEHTVAV
VDGLPLILSEV

Sequences:

>Translated_251_residues
MTKLRLKSKDEIKKIKASASLLALTLLEVERNIVPGISTKELDLIAYDFIIKNRAKPAFKGYRGFKGTICASVNEEVIHG
IPGKRKLADGDIVSIDCGVILDGFYSDMAKTFKVGNVDSSIDKLLEVTNASLYKGIAEMKVGNRILNISKAIEDYIKPFG
FGIVREYTGHGVGFELHEEPSVPNYYAPFFKNIRIQEGMVLAIEPMVNLRGHKVSIKSDGWTVFASDLSYSAHFEHTVAV
VDGLPLILSEV
>Mature_250_residues
TKLRLKSKDEIKKIKASASLLALTLLEVERNIVPGISTKELDLIAYDFIIKNRAKPAFKGYRGFKGTICASVNEEVIHGI
PGKRKLADGDIVSIDCGVILDGFYSDMAKTFKVGNVDSSIDKLLEVTNASLYKGIAEMKVGNRILNISKAIEDYIKPFGF
GIVREYTGHGVGFELHEEPSVPNYYAPFFKNIRIQEGMVLAIEPMVNLRGHKVSIKSDGWTVFASDLSYSAHFEHTVAVV
DGLPLILSEV

Specific function: Removes the amino-terminal methionine from nascent proteins

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family

Homologues:

Organism=Homo sapiens, GI164420681, Length=252, Percent_Identity=34.1269841269841, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI40385867, Length=249, Percent_Identity=32.1285140562249, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1786364, Length=248, Percent_Identity=40.7258064516129, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1788728, Length=214, Percent_Identity=24.2990654205607, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996291, Length=248, Percent_Identity=33.0645161290323, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323273, Length=244, Percent_Identity=34.0163934426229, Blast_Score=140, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355531, Length=242, Percent_Identity=35.9504132231405, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24583427, Length=244, Percent_Identity=34.8360655737705, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): AMPM_BORBU (O51132)

Other databases:

- EMBL:   AE000783
- PIR:   A70113
- RefSeq:   NP_212239.1
- ProteinModelPortal:   O51132
- SMR:   O51132
- MEROPS:   M24.001
- EnsemblBacteria:   EBBORT00000008780
- GeneID:   1194941
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0105
- NMPDR:   fig|224326.1.peg.489
- TIGR:   BB_0105
- GeneTree:   EBGT00050000006914
- HOGENOM:   HBG299384
- OMA:   EPMVCQK
- ProtClustDB:   CLSK507358
- BioCyc:   BBUR224326:BB_0105-MONOMER
- BRENDA:   3.4.11.18
- GO:   GO:0006508
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804:SF13
- PANTHER:   PTHR10804
- PRINTS:   PR00599
- TIGRFAMs:   TIGR00500

Pfam domain/function: PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: =3.4.11.18

Molecular weight: Translated: 27712; Mature: 27580

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS00680 MAP_1

Important sites: BINDING 79-79 BINDING 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLRLKSKDEIKKIKASASLLALTLLEVERNIVPGISTKELDLIAYDFIIKNRAKPAFK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHHHCCCCHHHH
GYRGFKGTICASVNEEVIHGIPGKRKLADGDIVSIDCGVILDGFYSDMAKTFKVGNVDSS
CCCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEECCEEEHHHHHHHHHHHCCCCCCHH
IDKLLEVTNASLYKGIAEMKVGNRILNISKAIEDYIKPFGFGIVREYTGHGVGFELHEEP
HHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCC
SVPNYYAPFFKNIRIQEGMVLAIEPMVNLRGHKVSIKSDGWTVFASDLSYSAHFEHTVAV
CCCCCHHHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCHHHEEHH
VDGLPLILSEV
HCCCHHHHHCC
>Mature Secondary Structure 
TKLRLKSKDEIKKIKASASLLALTLLEVERNIVPGISTKELDLIAYDFIIKNRAKPAFK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHHHCCCCHHHH
GYRGFKGTICASVNEEVIHGIPGKRKLADGDIVSIDCGVILDGFYSDMAKTFKVGNVDSS
CCCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEECCEEEHHHHHHHHHHHCCCCCCHH
IDKLLEVTNASLYKGIAEMKVGNRILNISKAIEDYIKPFGFGIVREYTGHGVGFELHEEP
HHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCC
SVPNYYAPFFKNIRIQEGMVLAIEPMVNLRGHKVSIKSDGWTVFASDLSYSAHFEHTVAV
CCCCCHHHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCHHHEEHH
VDGLPLILSEV
HCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685