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The map label for this gene is murI

Identifier: 15594446

GI number: 15594446

Start: 97863

End: 98648

Strand: Reverse

Name: murI

Synonym: BB0100

Alternate gene names: 15594446

Gene position: 98648-97863 (Counterclockwise)

Preceding gene: 15594449

Following gene: 15594445

Centisome position: 10.83

GC content: 26.46

Gene sequence:

>786_bases
ATGAAAAATTTCAAAGAAGTAATAATTATTTTTGATTCAGGAATAGGAGGGCTTTCTTATTTTAAATATATTAAAAGTAG
AATAGGGGGATGCCAATATGTTTATGTTGCCGATAATAAAAATTTCCCTTATGGAGAAAAAAGTCCTGAATATCTTCTAG
AAGCAGTTTTGTTTTTGATTGAGAAGCTTAAAAAAATCTATAATATTGGTGCATTAGTTTTGGCTTGTAATACAATTTCT
GTTAGTGTATACAATAAATTAAATTTTGTTTTTCCAGTAGTCTATACTTTGCCAGATGTAAGTTCAGTTTCAGATCTTGT
TTTAAAAAGAGTTCTTTTGATTGCAACAAATACTACTCTTGAAAGCAAATTTGTTAAGGATCAAGTAAATATACATAATG
ATTTGATTGTAAAAGCTGCTGGAGAGCTTGTTAATTTTGTTGAATATGGAGAGAATTACAAAAAATATGCTCTTAGATGT
TTAGAAGCTTTAAAATTTGAAGTTGTAAATACTGGTAGAGAAATTGTTTTTCTTGGATGCACGCATTATTTGCATCTTAA
GGTAATGATAGAAGATTTTTTAAAAATTCCTGTTTATGAGAATCGTGAATTAGTGGTAAAAAATCTTATTAGATCAATGA
ATTTTTCTGAACACAAAGGTAATTATTATAAGAATGATTTTGATTTTGTAGATGATGAGTTTTATTTGACCGAAAATAAA
AATTTGACTTTTTATCAAAATTTTTGCAAAAAATATAATCTTCGCTTTAAGGGAATGATAGTTTGA

Upstream 100 bases:

>100_bases
TTTTTAAAAAAAACATTTAACAATTTTTATTATTTTAATTCTTGATATTAACTTTGTTAATATTTATACTTTACTTTTTA
GGGGTGATTATTATTTTGTA

Downstream 100 bases:

>100_bases
ATTATCTTGAATTTGAAGTTATTTGGGGAGTTAATAATATATATTCTATTTTAGAGCTTAACAGTAAGTTAATTTATGAA
GGAATTTTTAAGGGTAAGGT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKNFKEVIIIFDSGIGGLSYFKYIKSRIGGCQYVYVADNKNFPYGEKSPEYLLEAVLFLIEKLKKIYNIGALVLACNTIS
VSVYNKLNFVFPVVYTLPDVSSVSDLVLKRVLLIATNTTLESKFVKDQVNIHNDLIVKAAGELVNFVEYGENYKKYALRC
LEALKFEVVNTGREIVFLGCTHYLHLKVMIEDFLKIPVYENRELVVKNLIRSMNFSEHKGNYYKNDFDFVDDEFYLTENK
NLTFYQNFCKKYNLRFKGMIV

Sequences:

>Translated_261_residues
MKNFKEVIIIFDSGIGGLSYFKYIKSRIGGCQYVYVADNKNFPYGEKSPEYLLEAVLFLIEKLKKIYNIGALVLACNTIS
VSVYNKLNFVFPVVYTLPDVSSVSDLVLKRVLLIATNTTLESKFVKDQVNIHNDLIVKAAGELVNFVEYGENYKKYALRC
LEALKFEVVNTGREIVFLGCTHYLHLKVMIEDFLKIPVYENRELVVKNLIRSMNFSEHKGNYYKNDFDFVDDEFYLTENK
NLTFYQNFCKKYNLRFKGMIV
>Mature_261_residues
MKNFKEVIIIFDSGIGGLSYFKYIKSRIGGCQYVYVADNKNFPYGEKSPEYLLEAVLFLIEKLKKIYNIGALVLACNTIS
VSVYNKLNFVFPVVYTLPDVSSVSDLVLKRVLLIATNTTLESKFVKDQVNIHNDLIVKAAGELVNFVEYGENYKKYALRC
LEALKFEVVNTGREIVFLGCTHYLHLKVMIEDFLKIPVYENRELVVKNLIRSMNFSEHKGNYYKNDFDFVDDEFYLTENK
NLTFYQNFCKKYNLRFKGMIV

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=189, Percent_Identity=30.6878306878307, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_BORBU (O51127)

Other databases:

- EMBL:   AE000783
- PIR:   D70112
- RefSeq:   NP_212234.1
- ProteinModelPortal:   O51127
- SMR:   O51127
- EnsemblBacteria:   EBBORT00000009013
- GeneID:   1194936
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0100
- NMPDR:   fig|224326.1.peg.484
- TIGR:   BB_0100
- GeneTree:   EBGT00050000007002
- HOGENOM:   HBG645102
- OMA:   GCTHYLH
- PhylomeDB:   O51127
- ProtClustDB:   CLSK507354
- BioCyc:   BBUR224326:BB_0100-MONOMER
- BRENDA:   5.1.1.3
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 30331; Mature: 30331

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFKEVIIIFDSGIGGLSYFKYIKSRIGGCQYVYVADNKNFPYGEKSPEYLLEAVLFLI
CCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EKLKKIYNIGALVLACNTISVSVYNKLNFVFPVVYTLPDVSSVSDLVLKRVLLIATNTTL
HHHHHHHCCCEEEEEECEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCH
ESKFVKDQVNIHNDLIVKAAGELVNFVEYGENYKKYALRCLEALKFEVVNTGREIVFLGC
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
THYLHLKVMIEDFLKIPVYENRELVVKNLIRSMNFSEHKGNYYKNDFDFVDDEFYLTENK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCC
NLTFYQNFCKKYNLRFKGMIV
CCHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKNFKEVIIIFDSGIGGLSYFKYIKSRIGGCQYVYVADNKNFPYGEKSPEYLLEAVLFLI
CCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EKLKKIYNIGALVLACNTISVSVYNKLNFVFPVVYTLPDVSSVSDLVLKRVLLIATNTTL
HHHHHHHCCCEEEEEECEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCH
ESKFVKDQVNIHNDLIVKAAGELVNFVEYGENYKKYALRCLEALKFEVVNTGREIVFLGC
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEC
THYLHLKVMIEDFLKIPVYENRELVVKNLIRSMNFSEHKGNYYKNDFDFVDDEFYLTENK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCC
NLTFYQNFCKKYNLRFKGMIV
CCHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685