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The map label for this gene is trmH [C]

Identifier: 15594398

GI number: 15594398

Start: 48603

End: 49259

Strand: Reverse

Name: trmH [C]

Synonym: BB0052

Alternate gene names: 15594398

Gene position: 49259-48603 (Counterclockwise)

Preceding gene: 15594400

Following gene: 15594395

Centisome position: 5.41

GC content: 27.85

Gene sequence:

>657_bases
ATGGATGATGATGTATTAAAAAGAATAGGTGTTTTGTCTAAATTTATTACAGATGAAAAAAAAGCTAGGATTGAGAAAGT
ATTAAATCTTCGTACCAATTATTTAACATTTGTTCTAGAAGACATTTTTCAGTCTCAAAATGCAAGTGCTACTATTAGAA
CAGGCGAAATTTTAGGACTTAGTGATGTTCATGTCATTGAAAAAAAGAATAAGCATACTTTAAATCCAGATGTTACTTTA
GGATCTTCACAATGGATAAATTTAAATAAGTATAAAAATGCTAAGTTTGCAATTGATAGGTTGAAATCTGATGGATATAG
TATAGTGGCCACATCTTTGAATCCCAAATCAATAAATCTTGAAAATTTGACAATTAACAATAAAATGGCGATATTTTTTG
GGACTGAATTAACAGGGCTTAGTGCAGAGGTATTGGGAGCTGCTGATTTATATGTAAAAATACCTATGTATGGGTTTACT
CAAAGTTATAATATTTCTGTAGCTGTTGCAATAGTTATGTATTCTTTATTGATGCGCTTAAGAAAAAGTAGTATTGATTA
CTTGCTAAATGAAGCTGAGAAATCAAATTTGAGATTAAAATATTATAGACAAGTTGTTAAAAATTATCAATTTATTGAGA
ATCTTATTAATTCTTAA

Upstream 100 bases:

>100_bases
CTTCAATTTTTACTTTCATTTTTGTATTATATAAGAAATAGTTTGCAATTTGAAATATATTAAAGGACTGATTTGTTATT
TTTGAAATTTAATTTTTATT

Downstream 100 bases:

>100_bases
GCTTCTTTTATTTTTTTCCAAGAATTTATAAATAGGTAAAAAATGGTATCGATTGCAATTATTATTAATGTAATAATTAG
AGAGCCTTTTGTAAGTTCGA

Product: SpoU protein

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MDDDVLKRIGVLSKFITDEKKARIEKVLNLRTNYLTFVLEDIFQSQNASATIRTGEILGLSDVHVIEKKNKHTLNPDVTL
GSSQWINLNKYKNAKFAIDRLKSDGYSIVATSLNPKSINLENLTINNKMAIFFGTELTGLSAEVLGAADLYVKIPMYGFT
QSYNISVAVAIVMYSLLMRLRKSSIDYLLNEAEKSNLRLKYYRQVVKNYQFIENLINS

Sequences:

>Translated_218_residues
MDDDVLKRIGVLSKFITDEKKARIEKVLNLRTNYLTFVLEDIFQSQNASATIRTGEILGLSDVHVIEKKNKHTLNPDVTL
GSSQWINLNKYKNAKFAIDRLKSDGYSIVATSLNPKSINLENLTINNKMAIFFGTELTGLSAEVLGAADLYVKIPMYGFT
QSYNISVAVAIVMYSLLMRLRKSSIDYLLNEAEKSNLRLKYYRQVVKNYQFIENLINS
>Mature_218_residues
MDDDVLKRIGVLSKFITDEKKARIEKVLNLRTNYLTFVLEDIFQSQNASATIRTGEILGLSDVHVIEKKNKHTLNPDVTL
GSSQWINLNKYKNAKFAIDRLKSDGYSIVATSLNPKSINLENLTINNKMAIFFGTELTGLSAEVLGAADLYVKIPMYGFT
QSYNISVAVAIVMYSLLMRLRKSSIDYLLNEAEKSNLRLKYYRQVVKNYQFIENLINS

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Escherichia coli, GI1790083, Length=181, Percent_Identity=32.0441988950276, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y052_BORBU (O51081)

Other databases:

- EMBL:   AE000783
- PIR:   D70106
- RefSeq:   NP_212186.1
- ProteinModelPortal:   O51081
- SMR:   O51081
- EnsemblBacteria:   EBBORT00000008967
- GeneID:   1194888
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0052
- NMPDR:   fig|224326.1.peg.436
- TIGR:   BB_0052
- GeneTree:   EBGT00050000006967
- HOGENOM:   HBG354186
- OMA:   FTESLNI
- PhylomeDB:   O51081
- ProtClustDB:   CLSK785729
- BioCyc:   BBUR224326:BB_0052-MONOMER
- InterPro:   IPR001537

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 24773; Mature: 24773

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDDVLKRIGVLSKFITDEKKARIEKVLNLRTNYLTFVLEDIFQSQNASATIRTGEILGL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEECC
SDVHVIEKKNKHTLNPDVTLGSSQWINLNKYKNAKFAIDRLKSDGYSIVATSLNPKSINL
CCCCEEECCCCCCCCCCEEECCCCEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEE
ENLTINNKMAIFFGTELTGLSAEVLGAADLYVKIPMYGFTQSYNISVAVAIVMYSLLMRL
EEEEECCEEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RKSSIDYLLNEAEKSNLRLKYYRQVVKNYQFIENLINS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDDDVLKRIGVLSKFITDEKKARIEKVLNLRTNYLTFVLEDIFQSQNASATIRTGEILGL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEECC
SDVHVIEKKNKHTLNPDVTLGSSQWINLNKYKNAKFAIDRLKSDGYSIVATSLNPKSINL
CCCCEEECCCCCCCCCCEEECCCCEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEE
ENLTINNKMAIFFGTELTGLSAEVLGAADLYVKIPMYGFTQSYNISVAVAIVMYSLLMRL
EEEEECCEEEEEECCCCCCCCHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RKSSIDYLLNEAEKSNLRLKYYRQVVKNYQFIENLINS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685