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The map label for this gene is smpB [H]

Identifier: 15594379

GI number: 15594379

Start: 31027

End: 31479

Strand: Direct

Name: smpB [H]

Synonym: BB0033

Alternate gene names: 15594379

Gene position: 31027-31479 (Clockwise)

Preceding gene: 15594378

Following gene: 15594386

Centisome position: 3.41

GC content: 24.06

Gene sequence:

>453_bases
TTGAACACAAACAATTTGCTTCTTGAAAACAAAAAAGCAAAATTTAATTACTTTATTGAAGAGAAAATAAGTTGTGGAAT
AGTGCTAAAAGGAACTGAGGTAAAATCAATAAAGGCAAAAAAATTATCATTTAACAATAGCTTTGCAAGCATAAAAAAAG
AAGAATTGTGGCTTGAAAACTTGCACGTATCAAAATATAAAGAAGGTAATATCTTCAATCATGATGAATTAAGGCCCAGA
AAACTTTTAATCAAAAAAAGAGAATTGCAAAGACTAAAAAAATTTAAAGAAAAAGAGGGATATACTTTAATTCCTATTAG
TTTTTATTTAAAAAAATCAATAATTAAAGTAGAAGTTGGAATATGTAAAGGAAAAAAGTTGTATGATAAAAGAGAAATAT
TAAAGCAAAAAAGCATAAAAAAAGATCTTAGTAGAGAAATAAAATACAAGTAA

Upstream 100 bases:

>100_bases
ATTTACTCAATGCATCAACCAAAGCCTCTTTTTTGAAAAACTGGAGTTCTCCAAACATTGGCTTTAGGTTGATTGTATCA
AAGGAATAAAAGGAATAGAT

Downstream 100 bases:

>100_bases
AAGCTCTTTTAATTACAATTGAGCAATTGCAATACAGAATTGCAATCACCCATTTTTGTAATAAATAAAATTAATAGCTT
TTTTTGAAAGACAGTTCAAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MNTNNLLLENKKAKFNYFIEEKISCGIVLKGTEVKSIKAKKLSFNNSFASIKKEELWLENLHVSKYKEGNIFNHDELRPR
KLLIKKRELQRLKKFKEKEGYTLIPISFYLKKSIIKVEVGICKGKKLYDKREILKQKSIKKDLSREIKYK

Sequences:

>Translated_150_residues
MNTNNLLLENKKAKFNYFIEEKISCGIVLKGTEVKSIKAKKLSFNNSFASIKKEELWLENLHVSKYKEGNIFNHDELRPR
KLLIKKRELQRLKKFKEKEGYTLIPISFYLKKSIIKVEVGICKGKKLYDKREILKQKSIKKDLSREIKYK
>Mature_150_residues
MNTNNLLLENKKAKFNYFIEEKISCGIVLKGTEVKSIKAKKLSFNNSFASIKKEELWLENLHVSKYKEGNIFNHDELRPR
KLLIKKRELQRLKKFKEKEGYTLIPISFYLKKSIIKVEVGICKGKKLYDKREILKQKSIKKDLSREIKYK

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=142, Percent_Identity=36.6197183098592, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17825; Mature: 17825

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNNLLLENKKAKFNYFIEEKISCGIVLKGTEVKSIKAKKLSFNNSFASIKKEELWLEN
CCCCCEEEECCCCEEEEEEECCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
LHVSKYKEGNIFNHDELRPRKLLIKKRELQRLKKFKEKEGYTLIPISFYLKKSIIKVEVG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHEEEEEE
ICKGKKLYDKREILKQKSIKKDLSREIKYK
EECCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTNNLLLENKKAKFNYFIEEKISCGIVLKGTEVKSIKAKKLSFNNSFASIKKEELWLEN
CCCCCEEEECCCCEEEEEEECCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
LHVSKYKEGNIFNHDELRPRKLLIKKRELQRLKKFKEKEGYTLIPISFYLKKSIIKVEVG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHEEEEEE
ICKGKKLYDKREILKQKSIKKDLSREIKYK
EECCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA