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The map label for this gene is priA

Identifier: 15594360

GI number: 15594360

Start: 12746

End: 14728

Strand: Direct

Name: priA

Synonym: BB0014

Alternate gene names: 15594360

Gene position: 12746-14728 (Clockwise)

Preceding gene: 15594359

Following gene: 15594363

Centisome position: 1.4

GC content: 26.37

Gene sequence:

>1983_bases
ATGGTTGAGCATTATCATTCAACTTATTACTATGAAATAGCAATAAATATTCCTTTAAATAAACTTTTTTTTTACAAATT
TAACTTAAACCTAGAAATTGGAATAAGAGTAATGGTTAATTTTAATGGCTCCAATAAAATTGGAATAATTATTAAAAAAT
ATTTTGAAAACGAATTTAAAGAAAAATTTGAATTTAAAATAAAAGAAATTATTAAAATAATAGATACAACTAAAATAATA
ACAGAACATAACATTGATTTAGCACATTGGATTAGTAAAAAAACATTTTCGGGATTTGGAGAAACTTTATTCTTTGGGTT
GCCCCAAAATTCAAAAGCTAAAAAAAATCAAACATTACCTTCAATAAATGAGCATCCGGATCATAAAAAATGCCTTGAGC
TAAACAACGAACAACAAAATATTTACAAAGAAATTATCGGGTCAGAAAAAACCAATGTTTTTTACCTTTTTGGAATACCT
GGATCTGGAAAAACTGAAATATTTATCAAATTGTGTGAATACTATTTAGCACTAGAACAACAAGTGCTTTTTTTGATTCC
CGAAATATCATTAGGATATCAAATAATAAAAAGAATAAAATATGCATTAAATATGCACCATAAAATTTATGAATATAACT
CCAAAGTTCCAAATTCTGATAAAAATTTAATATGGAATAAAGTTAAAAATGGAGAAAGCCTGGTTGTCATTGGCATCAAA
AGCGTTTTAATGCTGCCCTTCACCAAATTGAAATTAATAATTATGGACGAAGAACACGAAACTACATACAAATCTGAAAA
CATTCCAAGATTTCACTCAAGACATATATCATTTTTTTTACAAAAAAAATTTAATGCTAAATTTGTAATGGGAAGCGCAA
CACCTTCTCTTGAAGCATACCACGCAATGAAAAATAACCAAATAAAAAAAATAATAATGCAAAACAAATTCTCTCAAAGC
AAAATAGAAGATATAAAAATAATAAATATGAAAAAAGAACCTAGCACAATTTCATCAGAACTATTATACAGTATACAAAA
AAGTTTAAATGAAAAAAGACAATCTTTGATTTTCATTAATAAAAGAGGATATCTAAAAAATCTCGAATGCAACGAATGTG
GGCATATAATTTGTTGTCCAAATTGCTCATTTGGTTTGATTTACCATAAAAAAGAAAACAAACTTTTATGCCACTATTGT
AGTTATAAAACAAAAACAGCAAGCCATTGCCCTCAATGCGAATCAAAAGATATTAAATACAAAACTTATGGAATTCAGCT
TGTTGAAAAAGAACTAAAAAAATTTTTACCAAATGCAAAAATTGCAAGAATAGATTCTGATATTACAAAAATAGAAAATA
TAGATTCAATAAATAAGTTCGAAAATAAAGAAATAGACATACTTATCGGGACACAAATCATTGCAAAAGGATTTAATTTT
GAAAATATAAAAACACTGGGTATAATCAACGCTGACATTGGAATGGGACTGCCTGATTTTAGAAGTGGTGAAAGAATTTT
TACAACACTCTCACAAATTATGGGAAGAGCCGCACGATTCAAGGATGACAACATAATTATTATACAAACAAAAAATCCCA
ATTATTATGCCATAAAATATGCTTATAAGAATCAATATGAACAATTTTACGAAGAAGAACTAGATATACGAAAAAAATTG
AACTATCCTCCGTTTAACAAAATTATTAGAATAATATTTAGAAGCAAAAATGAAGAATCTGCTAAACAAAAATGTTGGGA
ATTTTTTGAAAAATCTAAAGAATTTTTGCAAGAAGAAATTGAACACTTAGGCCCATCAGAAGCTATTATGAAGAAAATAT
CTAAAAATTACAGATACAATATAATATACTTGTCAAAATCCTACAGCCTGCTTGAAAAACTAGTTAACAAGACAAAAGAA
AAGGTAAAAATGACAAGCACTGTTTATATTGAGATAGATTATTACCCAATATCATTAATTTAG

Upstream 100 bases:

>100_bases
CCATTAGTTTTTACATATCTTCCTACAAATCAAATAAAAAATCCAGAATTAAAAAAAGAAATATGGCAAGATTTAAAAAT
AATAAAAGGCATAATAAAAT

Downstream 100 bases:

>100_bases
GTAAAATCTAACCCTTCTTTACTTAAAGACTTAAGAAACGTTGAAAGCACATAAAGCGCTTTATCATTATGACCTCCCTC
GGGAATAATAAGATCAGCAT

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MVEHYHSTYYYEIAINIPLNKLFFYKFNLNLEIGIRVMVNFNGSNKIGIIIKKYFENEFKEKFEFKIKEIIKIIDTTKII
TEHNIDLAHWISKKTFSGFGETLFFGLPQNSKAKKNQTLPSINEHPDHKKCLELNNEQQNIYKEIIGSEKTNVFYLFGIP
GSGKTEIFIKLCEYYLALEQQVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVPNSDKNLIWNKVKNGESLVVIGIK
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFVMGSATPSLEAYHAMKNNQIKKIIMQNKFSQS
KIEDIKIINMKKEPSTISSELLYSIQKSLNEKRQSLIFINKRGYLKNLECNECGHIICCPNCSFGLIYHKKENKLLCHYC
SYKTKTASHCPQCESKDIKYKTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSINKFENKEIDILIGTQIIAKGFNF
ENIKTLGIINADIGMGLPDFRSGERIFTTLSQIMGRAARFKDDNIIIIQTKNPNYYAIKYAYKNQYEQFYEEELDIRKKL
NYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEEIEHLGPSEAIMKKISKNYRYNIIYLSKSYSLLEKLVNKTKE
KVKMTSTVYIEIDYYPISLI

Sequences:

>Translated_660_residues
MVEHYHSTYYYEIAINIPLNKLFFYKFNLNLEIGIRVMVNFNGSNKIGIIIKKYFENEFKEKFEFKIKEIIKIIDTTKII
TEHNIDLAHWISKKTFSGFGETLFFGLPQNSKAKKNQTLPSINEHPDHKKCLELNNEQQNIYKEIIGSEKTNVFYLFGIP
GSGKTEIFIKLCEYYLALEQQVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVPNSDKNLIWNKVKNGESLVVIGIK
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFVMGSATPSLEAYHAMKNNQIKKIIMQNKFSQS
KIEDIKIINMKKEPSTISSELLYSIQKSLNEKRQSLIFINKRGYLKNLECNECGHIICCPNCSFGLIYHKKENKLLCHYC
SYKTKTASHCPQCESKDIKYKTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSINKFENKEIDILIGTQIIAKGFNF
ENIKTLGIINADIGMGLPDFRSGERIFTTLSQIMGRAARFKDDNIIIIQTKNPNYYAIKYAYKNQYEQFYEEELDIRKKL
NYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEEIEHLGPSEAIMKKISKNYRYNIIYLSKSYSLLEKLVNKTKE
KVKMTSTVYIEIDYYPISLI
>Mature_660_residues
MVEHYHSTYYYEIAINIPLNKLFFYKFNLNLEIGIRVMVNFNGSNKIGIIIKKYFENEFKEKFEFKIKEIIKIIDTTKII
TEHNIDLAHWISKKTFSGFGETLFFGLPQNSKAKKNQTLPSINEHPDHKKCLELNNEQQNIYKEIIGSEKTNVFYLFGIP
GSGKTEIFIKLCEYYLALEQQVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVPNSDKNLIWNKVKNGESLVVIGIK
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFVMGSATPSLEAYHAMKNNQIKKIIMQNKFSQS
KIEDIKIINMKKEPSTISSELLYSIQKSLNEKRQSLIFINKRGYLKNLECNECGHIICCPNCSFGLIYHKKENKLLCHYC
SYKTKTASHCPQCESKDIKYKTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSINKFENKEIDILIGTQIIAKGFNF
ENIKTLGIINADIGMGLPDFRSGERIFTTLSQIMGRAARFKDDNIIIIQTKNPNYYAIKYAYKNQYEQFYEEELDIRKKL
NYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEEIEHLGPSEAIMKKISKNYRYNIIYLSKSYSLLEKLVNKTKE
KVKMTSTVYIEIDYYPISLI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=518, Percent_Identity=28.3783783783784, Blast_Score=274, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_BORBU (Q45032)

Other databases:

- EMBL:   X97449
- EMBL:   AE000783
- PIR:   F70101
- RefSeq:   NP_212148.1
- ProteinModelPortal:   Q45032
- SMR:   Q45032
- EnsemblBacteria:   EBBORT00000008991
- GeneID:   1194850
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0014
- NMPDR:   fig|224326.1.peg.398
- TIGR:   BB_0014
- GeneTree:   EBGT00050000006757
- HOGENOM:   HBG725563
- OMA:   TEVYLHV
- ProtClustDB:   CLSK507294
- BioCyc:   BBUR224326:BB_0014-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- InterPro:   IPR006935
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00595

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII

EC number: NA

Molecular weight: Translated: 77552; Mature: 77552

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEHYHSTYYYEIAINIPLNKLFFYKFNLNLEIGIRVMVNFNGSNKIGIIIKKYFENEFK
CCCEEECEEEEEEEEECCCCEEEEEEEEEEEEEEEEEEEEECCCCEEEEEEEHHHHHHHH
EKFEFKIKEIIKIIDTTKIITEHNIDLAHWISKKTFSGFGETLFFGLPQNSKAKKNQTLP
HHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
SINEHPDHKKCLELNNEQQNIYKEIIGSEKTNVFYLFGIPGSGKTEIFIKLCEYYLALEQ
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
QVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVPNSDKNLIWNKVKNGESLVVIGIK
CEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCEEEEEEHH
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFVMGSATPSLEAY
HHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHEEEECCCCEEEEECCCCCHHHH
HAMKNNQIKKIIMQNKFSQSKIEDIKIINMKKEPSTISSELLYSIQKSLNEKRQSLIFIN
HHHCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
KRGYLKNLECNECGHIICCPNCSFGLIYHKKENKLLCHYCSYKTKTASHCPQCESKDIKY
CCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEE
KTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSINKFENKEIDILIGTQIIAKGFNF
EEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHHCCCCEEEEEECCHHHHCCCCC
ENIKTLGIINADIGMGLPDFRSGERIFTTLSQIMGRAARFKDDNIIIIQTKNPNYYAIKY
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEE
AYKNQYEQFYEEELDIRKKLNYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEEI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EHLGPSEAIMKKISKNYRYNIIYLSKSYSLLEKLVNKTKEKVKMTSTVYIEIDYYPISLI
HHCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEEC
>Mature Secondary Structure
MVEHYHSTYYYEIAINIPLNKLFFYKFNLNLEIGIRVMVNFNGSNKIGIIIKKYFENEFK
CCCEEECEEEEEEEEECCCCEEEEEEEEEEEEEEEEEEEEECCCCEEEEEEEHHHHHHHH
EKFEFKIKEIIKIIDTTKIITEHNIDLAHWISKKTFSGFGETLFFGLPQNSKAKKNQTLP
HHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
SINEHPDHKKCLELNNEQQNIYKEIIGSEKTNVFYLFGIPGSGKTEIFIKLCEYYLALEQ
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
QVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVPNSDKNLIWNKVKNGESLVVIGIK
CEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEECCCCCEEEEEEHH
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFVMGSATPSLEAY
HHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHEEEECCCCEEEEECCCCCHHHH
HAMKNNQIKKIIMQNKFSQSKIEDIKIINMKKEPSTISSELLYSIQKSLNEKRQSLIFIN
HHHCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
KRGYLKNLECNECGHIICCPNCSFGLIYHKKENKLLCHYCSYKTKTASHCPQCESKDIKY
CCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEE
KTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSINKFENKEIDILIGTQIIAKGFNF
EEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHHCCCCEEEEEECCHHHHCCCCC
ENIKTLGIINADIGMGLPDFRSGERIFTTLSQIMGRAARFKDDNIIIIQTKNPNYYAIKY
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEE
AYKNQYEQFYEEELDIRKKLNYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEEI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EHLGPSEAIMKKISKNYRYNIIYLSKSYSLLEKLVNKTKEKVKMTSTVYIEIDYYPISLI
HHCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9228761; 9403685