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The map label for this gene is epsN

Identifier: 50812296

GI number: 50812296

Start: 3515062

End: 3516228

Strand: Reverse

Name: epsN

Synonym: BSU34230

Alternate gene names: 50812296

Gene position: 3516228-3515062 (Counterclockwise)

Preceding gene: 16080477

Following gene: 16080475

Centisome position: 83.41

GC content: 49.36

Gene sequence:

>1167_bases
ATGCATAAAAAAATCTACTTATCTCCCCCTCATATGAGCGGCAGGGAGCAGCACTATATTTCAGAAGCCTTTCGCTCAAA
CTGGATTGCGCCGCTTGGGCCGCTCGTGAATTCATTTGAAGAACAGCTGGCAGAACGAGTCGGTGTAAAAGCAGCAGCTG
CGGTCGGCTCAGGAACGGCGGCGATTCATCTCGCGCTGCGTTTGCTTGAGGTAAAAGAAGGTGACAGCGTGTTTTGCCAG
TCCTTCACATTTGTAGCAACCGCCAATCCGATTCTATATGAAAAAGCGGTGCCCGTCTTTATTGATTCTGAGCCTGATAC
GTGGAATATGTCGCCGACAGCCCTTGAAAGAGCATTGGAGGAAGCGAAAAGAAACGGAACACTGCCAAAAGCGGTAATTG
CCGTCAATCTATATGGGCAGAGCGCGAAAATGGATGAAATCGTAAGCCTGTGTGATGCATACGGAGTTCCTGTCATTGAG
GACGCAGCCGAATCTCTCGGCACAGTCTATAAAGGGAAGCAAAGCGGAACATTCGGGCGCTTCGGCATTTTTTCATTTAA
CGGGAACAAAATCATCACCACATCAGGGGGAGGGATGCTCGTTTCAAATGATGAAGCCGCAATTGAAAAAGCAAGATTTC
TCGCTTCGCAGGCACGAGAGCCGGCTGTTCATTATCAGCACAGTGAAATTGGACATAATTACAGGCTGAGCAATATCTTA
GCCGGCGTCGGCATTGCCCAGCTTGAGGTGCTGGATGAGCGAGTGGAGAAAAGAAGGACCATTTTCACGAGATACAAAAA
TGCGCTCGGTCACTTAGACGGCGTCCGCTTTATGCCAGAGTACGCAGCAGGCGTATCCAATCGCTGGCTCACCACGCTCA
CACTTGATAACGGGCTCAGCCCATATGACATAGTTCAACGTCTTGCTGAAGAAAACATTGAAGCCCGTCCGCTGTGGAAG
CCGCTCCATACCCAGCCGCTGTTCGATCCGGCTTTATTTTATTCTCATGAAGATACAGGAAGCGTATGCGAAGATTTGTT
CAAGCGAGGAATCTGTCTCCCATCGGGGTCCAATATGACAGAAGATGAGCAAGGCCGGGTCATTGAAGTGCTACTGCACT
TATTCCATACTGTCGAGGTGAAGAAATGGACAGCAAGCATTCGATGA

Upstream 100 bases:

>100_bases
GGCTCCGCGGTGATCCGTTCCATACCGGACAGGGTAACGGCGGCCGGTGCTCCGGCACGCATCATTTCTTCCATTCAAAC
ATCAAACAAAGGATGATTCT

Downstream 100 bases:

>100_bases
TCAGCCTGAAACAGAAACTGTCCGGGCTGCTCGACGTCATTCCGAAACAATCAGAGATTATATATGCTGACTATCCTCTA
TATGGGAATGTAGGGGATTT

Product: aminotransferase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MHKKIYLSPPHMSGREQHYISEAFRSNWIAPLGPLVNSFEEQLAERVGVKAAAAVGSGTAAIHLALRLLEVKEGDSVFCQ
SFTFVATANPILYEKAVPVFIDSEPDTWNMSPTALERALEEAKRNGTLPKAVIAVNLYGQSAKMDEIVSLCDAYGVPVIE
DAAESLGTVYKGKQSGTFGRFGIFSFNGNKIITTSGGGMLVSNDEAAIEKARFLASQAREPAVHYQHSEIGHNYRLSNIL
AGVGIAQLEVLDERVEKRRTIFTRYKNALGHLDGVRFMPEYAAGVSNRWLTTLTLDNGLSPYDIVQRLAEENIEARPLWK
PLHTQPLFDPALFYSHEDTGSVCEDLFKRGICLPSGSNMTEDEQGRVIEVLLHLFHTVEVKKWTASIR

Sequences:

>Translated_388_residues
MHKKIYLSPPHMSGREQHYISEAFRSNWIAPLGPLVNSFEEQLAERVGVKAAAAVGSGTAAIHLALRLLEVKEGDSVFCQ
SFTFVATANPILYEKAVPVFIDSEPDTWNMSPTALERALEEAKRNGTLPKAVIAVNLYGQSAKMDEIVSLCDAYGVPVIE
DAAESLGTVYKGKQSGTFGRFGIFSFNGNKIITTSGGGMLVSNDEAAIEKARFLASQAREPAVHYQHSEIGHNYRLSNIL
AGVGIAQLEVLDERVEKRRTIFTRYKNALGHLDGVRFMPEYAAGVSNRWLTTLTLDNGLSPYDIVQRLAEENIEARPLWK
PLHTQPLFDPALFYSHEDTGSVCEDLFKRGICLPSGSNMTEDEQGRVIEVLLHLFHTVEVKKWTASIR
>Mature_388_residues
MHKKIYLSPPHMSGREQHYISEAFRSNWIAPLGPLVNSFEEQLAERVGVKAAAAVGSGTAAIHLALRLLEVKEGDSVFCQ
SFTFVATANPILYEKAVPVFIDSEPDTWNMSPTALERALEEAKRNGTLPKAVIAVNLYGQSAKMDEIVSLCDAYGVPVIE
DAAESLGTVYKGKQSGTFGRFGIFSFNGNKIITTSGGGMLVSNDEAAIEKARFLASQAREPAVHYQHSEIGHNYRLSNIL
AGVGIAQLEVLDERVEKRRTIFTRYKNALGHLDGVRFMPEYAAGVSNRWLTTLTLDNGLSPYDIVQRLAEENIEARPLWK
PLHTQPLFDPALFYSHEDTGSVCEDLFKRGICLPSGSNMTEDEQGRVIEVLLHLFHTVEVKKWTASIR

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family

Homologues:

Organism=Escherichia coli, GI2367285, Length=382, Percent_Identity=30.1047120418848, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI145693159, Length=271, Percent_Identity=33.210332103321, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSN_BACSU (Q795J3)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   F70037
- RefSeq:   NP_391303.2
- HSSP:   Q8ZNF3
- ProteinModelPortal:   Q795J3
- SMR:   Q795J3
- EnsemblBacteria:   EBBACT00000003317
- GeneID:   936364
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34230
- GenoList:   BSU34230
- GeneTree:   EBGT00050000001120
- HOGENOM:   HBG660897
- OMA:   ESNYIAP
- PhylomeDB:   Q795J3
- ProtClustDB:   CLSK2503165
- BioCyc:   BSUB:BSU34230-MONOMER
- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11999
- PIRSF:   PIRSF000390

Pfam domain/function: PF01041 DegT_DnrJ_EryC1; SSF53383 PyrdxlP-dep_Trfase_major

EC number: NA

Molecular weight: Translated: 42924; Mature: 42924

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKIYLSPPHMSGREQHYISEAFRSNWIAPLGPLVNSFEEQLAERVGVKAAAAVGSGTA
CCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHH
AIHLALRLLEVKEGDSVFCQSFTFVATANPILYEKAVPVFIDSEPDTWNMSPTALERALE
HHHHHHHHHHHCCCCCCHHHCEEEEEECCCEEECCCCCEEECCCCCCCCCCHHHHHHHHH
EAKRNGTLPKAVIAVNLYGQSAKMDEIVSLCDAYGVPVIEDAAESLGTVYKGKQSGTFGR
HHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCE
FGIFSFNGNKIITTSGGGMLVSNDEAAIEKARFLASQAREPAVHYQHSEIGHNYRLSNIL
EEEEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEHHHHH
AGVGIAQLEVLDERVEKRRTIFTRYKNALGHLDGVRFMPEYAAGVSNRWLTTLTLDNGLS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCEEEEEEECCCCC
PYDIVQRLAEENIEARPLWKPLHTQPLFDPALFYSHEDTGSVCEDLFKRGICLPSGSNMT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCEECCCCCCCC
EDEQGRVIEVLLHLFHTVEVKKWTASIR
CCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHKKIYLSPPHMSGREQHYISEAFRSNWIAPLGPLVNSFEEQLAERVGVKAAAAVGSGTA
CCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHH
AIHLALRLLEVKEGDSVFCQSFTFVATANPILYEKAVPVFIDSEPDTWNMSPTALERALE
HHHHHHHHHHHCCCCCCHHHCEEEEEECCCEEECCCCCEEECCCCCCCCCCHHHHHHHHH
EAKRNGTLPKAVIAVNLYGQSAKMDEIVSLCDAYGVPVIEDAAESLGTVYKGKQSGTFGR
HHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCE
FGIFSFNGNKIITTSGGGMLVSNDEAAIEKARFLASQAREPAVHYQHSEIGHNYRLSNIL
EEEEEECCCEEEEECCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEHHHHH
AGVGIAQLEVLDERVEKRRTIFTRYKNALGHLDGVRFMPEYAAGVSNRWLTTLTLDNGLS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCEEEEEEECCCCC
PYDIVQRLAEENIEARPLWKPLHTQPLFDPALFYSHEDTGSVCEDLFKRGICLPSGSNMT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCEECCCCCCCC
EDEQGRVIEVLLHLFHTVEVKKWTASIR
CCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377